FastQCFastQC Report
Thu 26 May 2016
SRR935987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548919
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT16970.30915308087349863No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA15920.2900245755749027No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT15030.27381089013133086No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA11370.2071343859476535No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC11180.2036730373698123No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC10870.19802557390070302No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA6410.11677497044190492No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT6220.11331362186406374No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA5530.1007434612392721No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT250.002047406371.477821
TCTACAA300.00423850859.4888882
TATGGGA701.3418685E-859.4888844
CTATGGG655.4832526E-754.9128153
GTCCAGT952.6029738E-950.1598781
TCGTAGG602.1942978E-549.5740742
GGAAACA5250.045.3827481
TATGGGT802.2946406E-644.6166654
GGTATTC355.4563574E-542.49399628-29
TCATAGG1707.2759576E-1238.4928052
TAACAAC2550.037.3740271
GTTGTAG801.1890049E-437.2280351
TTAGGGA801.1979707E-437.1805534
TTGTAGG801.1979707E-437.1805532
TGCTAGG801.1979707E-437.1805532
AGTAATT801.1986133E-437.177177
GTATAGG2100.036.873481
TGATAGA650.001939796836.6085433
ATAAGGG1156.6587927E-736.2106253
GTCTATG1001.0532822E-535.738911