##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR935987_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548919
Sequences flagged as poor quality	0
Sequence length	125
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.029725697234017	31.0	30.0	34.0	15.0	34.0
2	28.900028966022308	31.0	30.0	34.0	12.0	34.0
3	28.90579848757285	31.0	30.0	34.0	12.0	34.0
4	32.01626287302862	37.0	35.0	37.0	12.0	37.0
5	32.190784068323374	37.0	35.0	37.0	12.0	37.0
6	32.3625835505785	37.0	35.0	37.0	16.0	37.0
7	32.31711053907772	37.0	35.0	37.0	13.0	37.0
8	32.06317325507042	37.0	35.0	37.0	10.0	37.0
9	33.24919887998047	39.0	35.0	39.0	8.0	39.0
10-11	33.32909591396909	39.0	35.0	39.0	9.0	39.0
12-13	33.32577575197798	39.0	35.0	39.0	8.0	39.0
14-15	34.33782944295971	40.0	35.0	41.0	10.0	41.0
16-17	34.46291620439445	40.0	35.0	41.0	14.5	41.0
18-19	34.69343928703506	40.0	35.0	41.0	17.5	41.0
20-21	34.8670659969868	40.0	35.0	41.0	20.0	41.0
22-23	34.65506112923765	40.0	34.5	41.0	18.5	41.0
24-25	34.386065157154334	40.0	34.0	41.0	16.5	41.0
26-27	34.20990255392873	40.0	34.0	41.0	12.0	41.0
28-29	34.0010675527719	40.0	34.0	41.0	4.0	41.0
30-31	33.767302643923784	40.0	34.0	41.0	2.0	41.0
32-33	33.436578074360696	39.0	33.0	41.0	2.0	41.0
34-35	33.10908804395548	39.0	33.0	41.0	2.0	41.0
36-37	32.91000858050094	39.0	33.0	41.0	2.0	41.0
38-39	32.7415411016926	39.0	33.0	41.0	2.0	41.0
40-41	32.481180283429794	39.0	33.0	41.0	2.0	41.0
42-43	32.193881064419344	38.5	33.0	41.0	2.0	41.0
44-45	31.82811580579284	38.0	32.5	40.5	2.0	41.0
46-47	31.46332427917416	37.0	32.0	40.0	2.0	41.0
48-49	31.068680442834008	36.5	32.0	40.0	2.0	41.0
50-51	30.653892468652025	36.0	31.0	39.0	2.0	41.0
52-53	30.31032356322153	35.0	31.0	39.0	2.0	41.0
54-55	29.9221050828993	35.0	31.0	37.5	2.0	41.0
56-57	29.573299521423017	35.0	30.5	37.0	2.0	40.0
58-59	29.20950632060468	35.0	30.0	36.5	2.0	39.5
60-61	28.906580934527682	35.0	30.0	36.0	2.0	39.0
62-63	28.613513104847893	35.0	29.5	35.5	2.0	38.5
64-65	28.413890756195357	35.0	29.0	35.0	2.0	37.0
66-67	28.30536290418076	35.0	29.5	35.0	2.0	37.0
68-69	28.220738396739772	35.0	30.0	35.0	2.0	36.0
70-71	28.057650582326353	35.0	29.5	35.0	2.0	36.0
72-73	27.91867379340121	35.0	29.0	35.0	2.0	35.5
74-75	27.778702322200544	35.0	29.0	35.0	2.0	35.0
76-77	27.623285949293063	35.0	29.0	35.0	2.0	35.0
78-79	27.479661844461567	35.0	29.0	35.0	2.0	35.0
80-81	27.31970563962989	35.0	27.5	35.0	2.0	35.0
82-83	27.172706719935	35.0	27.0	35.0	2.0	35.0
84-85	27.04937978098772	34.5	27.0	35.0	2.0	35.0
86-87	26.93333442639078	34.0	25.5	35.0	2.0	35.0
88-89	26.732235539305435	34.0	25.0	35.0	2.0	35.0
90-91	26.48400583692676	34.0	24.5	35.0	2.0	35.0
92-93	26.226527957676815	34.0	23.5	35.0	2.0	35.0
94-95	26.009951377161293	34.0	21.5	35.0	2.0	35.0
96-97	25.811152465117804	34.0	19.5	35.0	2.0	35.0
98-99	25.55819528928676	34.0	19.0	35.0	2.0	35.0
100-101	25.33764726671877	33.0	16.5	35.0	2.0	35.0
102-103	24.665413294129007	32.5	15.0	34.5	2.0	35.0
104-105	25.100835460240944	33.0	15.0	35.0	2.0	35.0
106-107	25.100939300698283	33.0	10.5	35.0	2.0	35.0
108-109	24.90865865455559	33.0	4.5	35.0	2.0	35.0
110-111	24.585975344267553	33.0	2.0	35.0	2.0	35.0
112-113	24.16778340702362	33.0	2.0	35.0	2.0	35.0
114-115	23.651175310018417	33.0	2.0	35.0	2.0	35.0
116-117	23.09223583078742	32.5	2.0	35.0	2.0	35.0
118-119	22.337047906886077	32.0	2.0	35.0	2.0	35.0
120-121	21.550847210608488	31.0	2.0	35.0	2.0	35.0
122-123	20.749810992150024	31.0	2.0	35.0	2.0	35.0
124-125	18.219853931090014	24.5	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3403881532473356
1101	2	0.5110987769211306
1101	3	0.2202207843740993
1101	4	0.4233825234044346
1101	5	0.24453467921254202
1101	6	0.38509640236247833
1101	7	0.21730646453587354
1101	8	0.3222119083111039
1101	9	0.19419364316815546
1101	10-11	0.3099572831829889
1101	12-13	0.2214191901105238
1101	14-15	0.30895027349052384
1101	16-17	0.40394773446449506
1101	18-19	0.5300407778232241
1101	20-21	0.4403534419346187
1101	22-23	0.33781824929325666
1101	24-25	0.6193452160471509
1101	26-27	0.5688269785271487
1101	28-29	0.45705971931312916
1101	30-31	0.3496050236130017
1101	32-33	0.182701336740152
1101	34-35	-0.1303304867669013
1101	36-37	-0.10575063092561265
1101	38-39	-0.07567175068312793
1101	40-41	-0.06018155539506864
1101	42-43	-0.09856306643690971
1101	44-45	-0.032621418138738534
1101	46-47	-0.12703434333627683
1101	48-49	0.03345339994389818
1101	50-51	0.007978227525065051
1101	52-53	0.023808303005029785
1101	54-55	-0.1553398639581296
1101	56-57	-0.11542221361521143
1101	58-59	-0.342346425288774
1101	60-61	-0.2403601444773642
1101	62-63	0.07183069646373852
1101	64-65	0.019787876185294095
1101	66-67	0.04128311235761828
1101	68-69	0.14051239787324832
1101	70-71	0.05010719015903575
1101	72-73	0.17202312794260521
1101	74-75	0.04684484662336175
1101	76-77	0.10252621264578465
1101	78-79	0.32392575056697837
1101	80-81	0.3296370149891992
1101	82-83	0.12592567057253135
1101	84-85	0.1303156587517833
1101	86-87	-0.030850102164919235
1101	88-89	-0.15812825230223737
1101	90-91	-0.2791765751406068
1101	92-93	-0.5052564207980907
1101	94-95	-0.511267303062862
1101	96-97	-0.5765274039737527
1101	98-99	-0.7071393233283487
1101	100-101	-0.4905309605410544
1101	102-103	-0.5895750553855414
1101	104-105	-0.6731574883255824
1101	106-107	-0.48770141733560024
1101	108-109	-0.5346897704932587
1101	110-111	-0.8529371814942337
1101	112-113	-0.5833151611983958
1101	114-115	-0.8075588183448055
1101	116-117	-0.9704876544170169
1101	118-119	-1.4203927276525725
1101	120-121	-1.9673328245308568
1101	122-123	-1.6998150691039946
1101	124-125	-1.2745406067358473
1102	1	0.12223209957787518
1102	2	0.12060786386645006
1102	3	0.21700616598616307
1102	4	-0.032645381544334384
1102	5	0.12334039497051208
1102	6	0.006496802703360061
1102	7	0.06900989517978928
1102	8	-0.024560591044782143
1102	9	-0.03912383508065176
1102	10-11	0.1606449974743711
1102	12-13	-0.027569057308063805
1102	14-15	0.07242313156769598
1102	16-17	0.2697701178800713
1102	18-19	0.32444195109948026
1102	20-21	0.3831492879024765
1102	22-23	0.39818648363141307
1102	24-25	0.4363983503991733
1102	26-27	0.4901857592711494
1102	28-29	0.39895777111961905
1102	30-31	0.1994989927352222
1102	32-33	0.0725402094436447
1102	34-35	-0.016839791719633013
1102	36-37	-0.09046930226953265
1102	38-39	-0.09234039923536841
1102	40-41	-0.10026221813759406
1102	42-43	-0.13218080034895507
1102	44-45	-0.11693083011329009
1102	46-47	-0.16147257787261537
1102	48-49	-0.10974774162218992
1102	50-51	-0.023693294761237382
1102	52-53	0.06464109131983165
1102	54-55	-0.023219444591145333
1102	56-57	-0.06649837747751164
1102	58-59	-0.20355597004965986
1102	60-61	-0.35051334423160796
1102	62-63	-0.3587725895025322
1102	64-65	-0.3681553229743848
1102	66-67	-0.21484388686944556
1102	68-69	-0.0407607654143014
1102	70-71	-0.08048222260827131
1102	72-73	-0.07790948794815478
1102	74-75	0.01058625019472359
1102	76-77	-0.07001971755220637
1102	78-79	-0.02082423456888094
1102	80-81	-0.04397021492934883
1102	82-83	0.04072540103609512
1102	84-85	0.16844416421187702
1102	86-87	0.08251474314891283
1102	88-89	0.22406054211677429
1102	90-91	0.28600367735161214
1102	92-93	0.3838124893630379
1102	94-95	0.45068544878524364
1102	96-97	0.21327084007563712
1102	98-99	0.23989676680780292
1102	100-101	0.469086139167608
1102	102-103	0.42523953921976343
1102	104-105	0.29100704781409803
1102	106-107	0.4448629443981531
1102	108-109	0.6226729343841626
1102	110-111	0.5709613856025015
1102	112-113	0.5176975823257912
1102	114-115	0.1744476426021393
1102	116-117	0.10497164756288768
1102	118-119	-0.03779764669254959
1102	120-121	0.060321415713310955
1102	122-123	0.28524944127117635
1102	124-125	0.5105912877485643
1103	1	0.33170644996131315
1103	2	0.4123917965594721
1103	3	0.37941775112502185
1103	4	0.3897542908553362
1103	5	0.3610814418024688
1103	6	0.11396087834925339
1103	7	0.425900670252382
1103	8	0.47556598180906207
1103	9	0.45589887357842684
1103	10-11	0.5919959765526173
1103	12-13	0.5761294759359217
1103	14-15	0.6662806254251947
1103	16-17	0.551590161063757
1103	18-19	0.4885231810897963
1103	20-21	0.7015191039995656
1103	22-23	0.6973749282743924
1103	24-25	0.6174980451352354
1103	26-27	0.6115598192815739
1103	28-29	0.7675788715589036
1103	30-31	0.6899073763254293
1103	32-33	0.7925982840471733
1103	34-35	0.7935466277576921
1103	36-37	0.7073156727881695
1103	38-39	0.7161083387588292
1103	40-41	0.8412815984062192
1103	42-43	0.8350554441600195
1103	44-45	0.8788699293238302
1103	46-47	0.8130006556915248
1103	48-49	0.805167380111115
1103	50-51	0.5959813385497554
1103	52-53	0.5961930978718399
1103	54-55	0.6147505501970336
1103	56-57	0.4671122993149872
1103	58-59	0.6469205395177582
1103	60-61	0.6949840126293836
1103	62-63	0.335635868542294
1103	64-65	0.36581645872466595
1103	66-67	0.7662539465011129
1103	68-69	0.7476094229558861
1103	70-71	0.7491544388465705
1103	72-73	0.739337924753805
1103	74-75	0.8034460521454356
1103	76-77	0.7502561368145564
1103	78-79	0.6903607107069725
1103	80-81	0.7847264569491408
1103	82-83	0.8591212762501534
1103	84-85	0.8207541187945573
1103	86-87	0.6484275567889988
1103	88-89	0.6783193463755772
1103	90-91	0.47886347930232276
1103	92-93	0.5291106801157746
1103	94-95	0.6738240832875135
1103	96-97	0.5395235390556081
1103	98-99	0.8942411938795054
1103	100-101	0.6955715088347958
1103	102-103	0.7312833929908926
1103	104-105	0.8542729997164109
1103	106-107	0.5716219347935052
1103	108-109	0.5779536283012199
1103	110-111	0.6786786069560904
1103	112-113	0.8623294042303407
1103	114-115	0.5091434954798117
1103	116-117	0.496132022814173
1103	118-119	0.3464068056937215
1103	120-121	0.3518242822161781
1103	122-123	0.35966869523588585
1103	124-125	0.08063633279360971
1104	1	0.08310600010591074
1104	2	0.21388238886908795
1104	3	0.45447755333145423
1104	4	0.15105423016056108
1104	5	0.3172241102304696
1104	6	0.25732015572438627
1104	7	0.05439151248166496
1104	8	0.37173150003534516
1104	9	0.34376170338313017
1104	10-11	0.1635881433561508
1104	12-13	0.26034794142915985
1104	14-15	0.33215052438623616
1104	16-17	0.3638871013329492
1104	18-19	0.299486621412548
1104	20-21	0.45744666299844994
1104	22-23	0.42983235267369935
1104	24-25	0.14095836146212548
1104	26-27	0.4411030018300366
1104	28-29	0.2696567750899135
1104	30-31	0.13764471075364781
1104	32-33	0.2336208125201651
1104	34-35	0.43015628893802926
1104	36-37	0.27494750541339386
1104	38-39	0.10635879396964754
1104	40-41	0.41445896996535936
1104	42-43	0.2900591929550558
1104	44-45	0.08968074013464289
1104	46-47	0.25869311168781195
1104	48-49	0.1907479077986416
1104	50-51	0.2972952019508064
1104	52-53	0.21111240952166455
1104	54-55	0.20706284267444985
1104	56-57	0.19836564645340715
1104	58-59	0.20854358925177152
1104	60-61	0.16394309720934075
1104	62-63	-0.08634099603694878
1104	64-65	0.04383870157518288
1104	66-67	0.09095761317968254
1104	68-69	0.2531826315674053
1104	70-71	0.2507467841641464
1104	72-73	0.07902159785887619
1104	74-75	0.3458574199200761
1104	76-77	0.3204433980520136
1104	78-79	0.4083683154054647
1104	80-81	0.37458061097106565
1104	82-83	0.4182495921486158
1104	84-85	0.5488923957769565
1104	86-87	0.48910466057706614
1104	88-89	0.4223712582610446
1104	90-91	0.46275437151554044
1104	92-93	0.5415441836324995
1104	94-95	0.6092814362811936
1104	96-97	0.46216090561635426
1104	98-99	0.7137277874744186
1104	100-101	0.5172154447125479
1104	102-103	0.6799240085073315
1104	104-105	0.6676178174535714
1104	106-107	0.6372436992263673
1104	108-109	0.6284418642253868
1104	110-111	0.633193227256708
1104	112-113	0.49352830458380126
1104	114-115	0.5060123590593761
1104	116-117	0.4080683647042811
1104	118-119	0.42376586885199075
1104	120-121	0.6094887829123827
1104	122-123	0.6737829085244691
1104	124-125	0.34304395177322533
1105	1	0.28341378389371386
1105	2	0.2455189134461797
1105	3	-0.03396788515064131
1105	4	0.07260146027539349
1105	5	0.14087683822190655
1105	6	0.2687649151226026
1105	7	0.14112842998142128
1105	8	0.1140259251283382
1105	9	0.18756058644218143
1105	10-11	0.2786742788958847
1105	12-13	0.3873256465310675
1105	14-15	0.2735972771152433
1105	16-17	0.45415289003312864
1105	18-19	0.2920639981216624
1105	20-21	0.2167485917993872
1105	22-23	0.24741640197316173
1105	24-25	0.25296496986047146
1105	26-27	0.6024200529167416
1105	28-29	0.5058867446493807
1105	30-31	0.398487247756961
1105	32-33	0.4847804918304064
1105	34-35	0.20533898277255958
1105	36-37	0.22467587186191906
1105	38-39	0.13976735583216282
1105	40-41	0.16440491353720432
1105	42-43	0.14661623769012522
1105	44-45	0.23993649461035105
1105	46-47	0.2227227819098161
1105	48-49	0.10591563475962573
1105	50-51	0.13389866764355673
1105	52-53	0.21412183223279868
1105	54-55	0.2822680037119305
1105	56-57	0.3337415244480475
1105	58-59	0.47919398943826863
1105	60-61	0.320422942607955
1105	62-63	0.15203180411981165
1105	64-65	0.15173786606551687
1105	66-67	0.2014599325383024
1105	68-69	0.29053815731684907
1105	70-71	0.3616207414663286
1105	72-73	0.20412675450427287
1105	74-75	0.1696207946628867
1105	76-77	0.2918940023015253
1105	78-79	0.254888601769391
1105	80-81	0.1177205123856524
1105	82-83	0.19411643765196374
1105	84-85	0.1036583145504153
1105	86-87	0.10310544439527547
1105	88-89	0.2094902871373101
1105	90-91	0.40603566427502
1105	92-93	0.23302303236572186
1105	94-95	-7.335305923490409E-4
1105	96-97	0.025557270997268233
1105	98-99	0.20519025035285665
1105	100-101	0.27202308829149047
1105	102-103	0.2863518245938472
1105	104-105	0.36551755629345095
1105	106-107	0.04678955765933779
1105	108-109	0.23537223616740022
1105	110-111	0.08994217231172641
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2114	22-23	-0.12141808834470424
2114	24-25	-0.09108569388328647
2114	26-27	-0.19267189690666697
2114	28-29	-0.35694969427401446
2114	30-31	-0.24330425145141987
2114	32-33	-0.24871725509444786
2114	34-35	-0.24165285744179243
2114	36-37	-0.16148293948870673
2114	38-39	-0.0443149727514367
2114	40-41	-0.11037891303690373
2114	42-43	-0.10828572309419116
2114	44-45	-0.11580580230841875
2114	46-47	0.0316985461285455
2114	48-49	-0.09829213530492353
2114	50-51	-0.19130532699777802
2114	52-53	-0.14028079838466567
2114	54-55	-0.08973297059083407
2114	56-57	0.00509702316927374
2114	58-59	-0.01066717241777937
2114	60-61	-0.033644771434182985
2114	62-63	-0.03993628746464495
2114	64-65	0.15627446331311035
2114	66-67	0.17165235733430606
2114	68-69	0.11211896355238338
2114	70-71	0.23188623896967542
2114	72-73	0.27887404472470934
2114	74-75	0.1833828212126356
2114	76-77	0.10832927206160292
2114	78-79	0.08487276934947019
2114	80-81	0.25908575413452795
2114	82-83	0.3495177286031286
2114	84-85	0.2480986994000105
2114	86-87	0.36047360905244474
2114	88-89	0.4403695953823501
2114	90-91	0.5799182607918851
2114	92-93	0.6270067127944152
2114	94-95	0.37817890394668296
2114	96-97	0.26136898698801403
2114	98-99	0.20193242548378265
2114	100-101	0.196149260499503
2114	102-103	0.4346589735403832
2114	104-105	0.39386367735056993
2114	106-107	0.3354571986287702
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2114	112-113	0.7857108602204903
2114	114-115	0.6175845452252062
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2114	118-119	0.34313334589852573
2114	120-121	0.5666737590221764
2114	122-123	0.7152153909999726
2114	124-125	0.4521026749121262
2115	1	-0.3389635547414436
2115	2	-0.4324738982038667
2115	3	-0.26147549626771394
2115	4	-0.3249750239251554
2115	5	-0.4702389266724545
2115	6	-0.259815712981073
2115	7	-0.5827745351474896
2115	8	-0.34109008059930446
2115	9	-0.4045633798725703
2115	10-11	-0.3627406689977377
2115	12-13	-0.49522035393489006
2115	14-15	-0.6628750911921415
2115	16-17	-0.4926399697835748
2115	18-19	-0.46763674034647806
2115	20-21	-0.3926944288782508
2115	22-23	-0.32002804276213936
2115	24-25	-0.3894565040915836
2115	26-27	-0.4668607456026663
2115	28-29	-0.5631578016547394
2115	30-31	-0.6456572312709099
2115	32-33	-0.41900922930035733
2115	34-35	-0.3571546364543181
2115	36-37	-0.38027181234281926
2115	38-39	-0.42884657009065563
2115	40-41	-0.5073137984235672
2115	42-43	-0.41087943647291425
2115	44-45	-0.6641575403157169
2115	46-47	-0.5101230821712122
2115	48-49	-0.44884448743708916
2115	50-51	-0.4747924034624731
2115	52-53	-0.5149780271938589
2115	54-55	-0.5419322072789683
2115	56-57	-0.5697995101403244
2115	58-59	-0.7967708489023906
2115	60-61	-0.7479998873401854
2115	62-63	-0.712557719108819
2115	64-65	-0.7907376644875939
2115	66-67	-0.8526117205989436
2115	68-69	-1.1535054073298774
2115	70-71	-1.2285876840337302
2115	72-73	-1.172950105343844
2115	74-75	-1.09916812805319
2115	76-77	-1.0846390193446744
2115	78-79	-1.0433061957081406
2115	80-81	-0.9471271212964503
2115	82-83	-0.8739660843960628
2115	84-85	-0.9430450181110288
2115	86-87	-0.8826376179046314
2115	88-89	-1.1221135839959402
2115	90-91	-0.9245695340590423
2115	92-93	-0.9728050021990882
2115	94-95	-1.0510860110442763
2115	96-97	-1.0842378374693311
2115	98-99	-1.1144727401159038
2115	100-101	-1.2144280762159667
2115	102-103	-1.1175236279502165
2115	104-105	-0.9564693444701469
2115	106-107	-1.249009341313716
2115	108-109	-1.3210442692893096
2115	110-111	-1.1725759229064252
2115	112-113	-0.8059670908317642
2115	114-115	-0.8474555805665531
2115	116-117	-0.9123460816977094
2115	118-119	-0.6114827624824386
2115	120-121	-0.6540562400665948
2115	122-123	-0.5909654855160049
2115	124-125	-0.3662425573554948
2116	1	-0.5761780734799196
2116	2	-0.509076550038948
2116	3	-0.9820145969358371
2116	4	-0.7871939893707776
2116	5	-0.8101984749509157
2116	6	-0.7540946381453857
2116	7	-0.6813018496452479
2116	8	-0.8645330141692291
2116	9	-0.8433122522195902
2116	10-11	-1.0791398054312324
2116	12-13	-1.0691422005272244
2116	14-15	-1.142433551811358
2116	16-17	-1.3065636483127392
2116	18-19	-1.47537773856034
2116	20-21	-1.3774787023395518
2116	22-23	-1.7426734447604488
2116	24-25	-1.4762865823642812
2116	26-27	-1.372275290542099
2116	28-29	-1.3828387677245004
2116	30-31	-1.4097980262675875
2116	32-33	-1.5283146163662487
2116	34-35	-1.6571550624861402
2116	36-37	-1.473678224334762
2116	38-39	-1.5570917343632011
2116	40-41	-1.4093547038690062
2116	42-43	-1.4925284352448784
2116	44-45	-1.3520466271910614
2116	46-47	-1.3960945998137184
2116	48-49	-1.6048243233509325
2116	50-51	-1.4788056232299205
2116	52-53	-1.5422866670127142
2116	54-55	-1.6121534349535693
2116	56-57	-1.4186689814454816
2116	58-59	-1.5612663153912614
2116	60-61	-1.8018668184306037
2116	62-63	-1.690560098496551
2116	64-65	-1.5645509794731538
2116	66-67	-1.6464320225063958
2116	68-69	-1.4986486978079583
2116	70-71	-1.455663177937744
2116	72-73	-1.4986152208996515
2116	74-75	-1.463598411673221
2116	76-77	-2.351499494987902
2116	78-79	-2.144797626610572
2116	80-81	-2.238941878399732
2116	82-83	-2.433925803087252
2116	84-85	-2.275386584864357
2116	86-87	-2.1090090942220563
2116	88-89	-2.187221897261402
2116	90-91	-2.0836024205204957
2116	92-93	-2.088622818210858
2116	94-95	-2.222808787877092
2116	96-97	-2.1621681109711126
2116	98-99	-2.3200485381001315
2116	100-101	-2.38164183874796
2116	102-103	-2.5319088054510495
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2116	108-109	-2.5725617413868846
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2116	114-115	-2.6758861751898166
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2116	118-119	-1.7188475745970777
2116	120-121	-1.668283572777213
2116	122-123	-1.3191055264363598
2116	124-125	-0.8144173837741526
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2201	2	0.7286831897165378
2201	3	0.7553886338123235
2201	4	1.017105862236761
2201	5	0.8262103427522618
2201	6	0.91246284028405
2201	7	1.1685534994723739
2201	8	0.9025600043254727
2201	9	1.0790662064472514
2201	10-11	1.150972286648333
2201	12-13	0.944964966786344
2201	14-15	0.9262223174564355
2201	16-17	1.0688120980618123
2201	18-19	0.9684738576822625
2201	20-21	0.5833363690257869
2201	22-23	0.7445127037703827
2201	24-25	0.9447964425828843
2201	26-27	0.9360555837027036
2201	28-29	1.1272070207392915
2201	30-31	1.1174505572268174
2201	32-33	1.0746988377895192
2201	34-35	1.091955076912413
2201	36-37	1.2303748242055264
2201	38-39	1.227660834266544
2201	40-41	1.306906483807218
2201	42-43	1.323896252403813
2201	44-45	1.516410726566125
2201	46-47	1.5159563929756246
2201	48-49	1.6570587219278288
2201	50-51	1.6041077824970955
2201	52-53	1.4061155372718908
2201	54-55	1.530335096751731
2201	56-57	1.650752146760805
2201	58-59	1.7043117163119206
2201	60-61	1.468429133462564
2201	62-63	1.491099948633238
2201	64-65	1.5016382710390204
2201	66-67	1.488067177642705
2201	68-69	1.5483391893572964
2201	70-71	1.6353292160661255
2201	72-73	1.7022701921636845
2201	74-75	1.5395529867598299
2201	76-77	1.4610953681761742
2201	78-79	1.4729774183236835
2201	80-81	1.4354919782370494
2201	82-83	1.3488027827227036
2201	84-85	1.3938563594341105
2201	86-87	1.3708972109750732
2201	88-89	1.388013504577195
2201	90-91	1.1989595590998938
2201	92-93	1.3412875109493214
2201	94-95	1.2367242629787398
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2202	3	0.49606839629823796
2202	4	0.3802800423249977
2202	5	0.45601988229494594
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2202	7	0.6338424013131316
2202	8	0.4168570676965899
2202	9	0.39683317313105704
2202	10-11	0.5357670047627678
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2202	56-57	0.04580956703414074
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2202	68-69	0.0812804400742344
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2203	3	0.03946444703559138
2203	4	0.13338666668666832
2203	5	0.06474636326843353
2203	6	0.1218567323184061
2203	7	0.08731286779023151
2203	8	0.1018005933839845
2203	9	0.27894259793104936
2203	10-11	0.19510549968831725
2203	12-13	-0.06150672046990735
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2203	70-71	0.6779679382098109
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2204	1	0.6777657350528585
2204	2	0.7712148131244234
2204	3	0.5952590763211845
2204	4	0.7512451365397297
2204	5	0.5623717643831156
2204	6	0.7185380361522178
2204	7	0.6255868080030353
2204	8	0.8616688588796819
2204	9	0.8848875396638647
2204	10-11	0.6441276243294283
2204	12-13	0.6309202344363598
2204	14-15	0.7457662400721006
2204	16-17	0.35385258477778336
2204	18-19	0.39891986809939084
2204	20-21	0.4122819636333901
2204	22-23	0.499990447018952
2204	24-25	0.5735573302267767
2204	26-27	0.6890358436930839
2204	28-29	0.887317319684449
2204	30-31	0.9064766875721588
2204	32-33	1.0040891297315682
2204	34-35	1.135935468211052
2204	36-37	1.1340637111491105
2204	38-39	1.0481528025774836
2204	40-41	1.0592196977959318
2204	42-43	1.009615165171354
2204	44-45	0.9380678369088322
2204	46-47	0.9309605510707826
2204	48-49	0.8184847297187865
2204	50-51	0.8914128991426082
2204	52-53	0.8575052164420214
2204	54-55	0.6401254412170871
2204	56-57	0.6845360133516003
2204	58-59	0.8873913328917773
2204	60-61	0.781858467729645
2204	62-63	0.7977021283504833
2204	64-65	0.8176342442521332
2204	66-67	0.7758572594915272
2204	68-69	0.8739215415260695
2204	70-71	0.7324325556730749
2204	72-73	0.7903754138558092
2204	74-75	0.8925306205848393
2204	76-77	0.849998944138008
2204	78-79	0.8862325502884936
2204	80-81	0.8825773723807053
2204	82-83	0.9415580678804893
2204	84-85	0.8561638833978691
2204	86-87	0.68214595260417
2204	88-89	0.6040996428010317
2204	90-91	0.6318190154836643
2204	92-93	0.588421926846376
2204	94-95	0.20736811118638343
2204	96-97	0.5148766340861215
2204	98-99	0.46667362631193754
2204	100-101	0.42437272941020865
2204	102-103	0.5425344827903658
2204	104-105	0.3379216483650609
2204	106-107	0.353644602622623
2204	108-109	0.20354647921665148
2204	110-111	0.3344937769648091
2204	112-113	0.12685425165841124
2204	114-115	-0.044439765200223746
2204	116-117	0.1967205145316946
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2211	74-75	0.12887007681596074
2211	76-77	0.25028159284108753
2211	78-79	0.16756592991972852
2211	80-81	0.0697706777038043
2211	82-83	0.07020628483246938
2211	84-85	0.16014041036061144
2211	86-87	0.2899899749660584
2211	88-89	0.2710971079907978
2211	90-91	0.3676890109696167
2211	92-93	0.27003378836934644
2211	94-95	-0.0043441010432090366
2211	96-97	0.16153936543095782
2211	98-99	0.20988137658104478
2211	100-101	0.3306986435044479
2211	102-103	0.17561688148476762
2211	104-105	0.23271174524342442
2211	106-107	0.23563147716687993
2211	108-109	0.13953175648322258
2211	110-111	-0.0032519780961308697
2211	112-113	-0.12073142839992101
2211	114-115	0.03751169144757682
2211	116-117	-0.04018354463702423
2211	118-119	-0.0027989662784655422
2211	120-121	-0.49799316170783214
2211	122-123	-0.6654840409233103
2211	124-125	-0.974518148921689
2212	1	0.027704538081589902
2212	2	-0.05406195622868637
2212	3	0.11772631692554825
2212	4	-0.04472761870695763
2212	5	-0.033130776847052346
2212	6	0.3623647484573027
2212	7	0.06981596818697255
2212	8	0.13470969143508427
2212	9	-0.16180194621242805
2212	10-11	0.10133884031670704
2212	12-13	0.029852572022363688
2212	14-15	0.10630698619110035
2212	16-17	0.2767900393986764
2212	18-19	0.07260171299791551
2212	20-21	0.06198012772425443
2212	22-23	0.16221082526462993
2212	24-25	0.02523246766065057
2212	26-27	0.11938331802146962
2212	28-29	0.05558617146331102
2212	30-31	-0.06828111763477551
2212	32-33	-0.38416658371447454
2212	34-35	-0.2410786727840062
2212	36-37	-0.17673461544218583
2212	38-39	-0.19483789686586306
2212	40-41	-0.22783212946956866
2212	42-43	-0.29434984537388686
2212	44-45	-0.21149825236267716
2212	46-47	-0.2099100363509976
2212	48-49	-0.14762874398725856
2212	50-51	-0.14532395388093988
2212	52-53	0.011099241958049788
2212	54-55	0.016800751523764035
2212	56-57	0.17178699008292497
2212	58-59	0.11052240504888644
2212	60-61	0.03792526577964139
2212	62-63	0.22903843509598332
2212	64-65	0.17898887551444886
2212	66-67	0.17445837279235477
2212	68-69	0.2080943451771411
2212	70-71	0.30492918465347074
2212	72-73	0.10448667479001728
2212	74-75	0.2832293724432233
2212	76-77	0.41389203959836607
2212	78-79	0.3369548128477007
2212	80-81	0.28899994265349704
2212	82-83	0.25951886032940763
2212	84-85	0.4037709780975902
2212	86-87	0.2914050197825411
2212	88-89	0.27117152331135586
2212	90-91	0.12241957559153605
2212	92-93	0.27496827404410595
2212	94-95	0.34406900666748896
2212	96-97	0.44408453091563516
2212	98-99	0.3808001000525607
2212	100-101	0.25124269448990333
2212	102-103	0.20094751048458903
2212	104-105	0.2087898155676342
2212	106-107	0.1961982796270867
2212	108-109	0.11148756416634598
2212	110-111	0.20544492311147167
2212	112-113	0.36096067215421
2212	114-115	0.1583483273212991
2212	116-117	0.3593696173218639
2212	118-119	0.36294022013710503
2212	120-121	0.017327164519706173
2212	122-123	-0.18948327023440825
2212	124-125	-0.03174526101885178
2213	1	-0.10489335739320893
2213	2	-0.29195689175147876
2213	3	-0.09939974561700282
2213	4	-0.12164862149097289
2213	5	-0.1016019963526702
2213	6	-0.0450651287899575
2213	7	-0.03277120917421428
2213	8	0.3949778465039415
2213	9	0.055233113478699636
2213	10-11	0.031155419013948915
2213	12-13	0.14216862829583476
2213	14-15	0.16838272752729466
2213	16-17	0.2206294834049629
2213	18-19	0.3322126252509392
2213	20-21	0.23140889945540266
2213	22-23	0.2885783958929622
2213	24-25	0.208571193755553
2213	26-27	0.19722614598563126
2213	28-29	0.16683378685155503
2213	30-31	0.08027935207476133
2213	32-33	-0.08767255282743491
2213	34-35	-0.10980200389282402
2213	36-37	-0.07914248348131281
2213	38-39	0.09817106185928992
2213	40-41	-0.0336050807257422
2213	42-43	-0.006836447731870976
2213	44-45	-0.11681821164732753
2213	46-47	-0.035976623474432756
2213	48-49	0.051114742567911264
2213	50-51	0.11740464865231104
2213	52-53	0.18447147058417457
2213	54-55	0.32608405351167136
2213	56-57	0.10554365519917397
2213	58-59	0.2192050882174037
2213	60-61	0.22513161306000384
2213	62-63	0.09140673374711739
2213	64-65	0.26255140121432774
2213	66-67	0.26260643106114756
2213	68-69	0.21319387533279155
2213	70-71	0.21903091910041184
2213	72-73	0.20361068808317384
2213	74-75	-0.02353281351267711
2213	76-77	0.20094031155533543
2213	78-79	0.13454593366578038
2213	80-81	0.27339295363450944
2213	82-83	0.19644208762756676
2213	84-85	0.16804999958100453
2213	86-87	0.19054184088441772
2213	88-89	0.11314001610190871
2213	90-91	0.11928503293142256
2213	92-93	0.3056985887791548
2213	94-95	0.3235478636716742
2213	96-97	0.2538784600143025
2213	98-99	0.05570926572493562
2213	100-101	0.15766142894358381
2213	102-103	0.1382313787124616
2213	104-105	0.12907468819734547
2213	106-107	0.19821550950028666
2213	108-109	0.09534129899077826
2213	110-111	0.37210316358819284
2213	112-113	0.29160301746621897
2213	114-115	0.495193696624348
2213	116-117	0.8057487149780336
2213	118-119	0.5888851330777101
2213	120-121	0.1781982977411367
2213	122-123	0.28203115203230666
2213	124-125	-0.06645273740866742
2214	1	-0.47458330553400074
2214	2	-0.36764926734455017
2214	3	-0.5024671625871022
2214	4	-0.3837031288284969
2214	5	-0.4921459663405301
2214	6	-0.25062620128251467
2214	7	-0.20352040921834913
2214	8	-0.30876263677973625
2214	9	-0.5008189664065483
2214	10-11	-0.5598740414296159
2214	12-13	-0.3578589563891512
2214	14-15	-0.6419173938453184
2214	16-17	-0.5816159099526459
2214	18-19	-0.48804556740049776
2214	20-21	-0.566147097455115
2214	22-23	-0.5388793995390202
2214	24-25	-0.6451472217801495
2214	26-27	-0.8245381704658783
2214	28-29	-0.8069883470996686
2214	30-31	-0.4633596195681804
2214	32-33	-0.532448013491674
2214	34-35	-0.611126068772009
2214	36-37	-0.568274117598051
2214	38-39	-0.24393420129860743
2214	40-41	-0.3252855397348995
2214	42-43	-0.3978493573644428
2214	44-45	-0.4465655359328835
2214	46-47	-0.4779384462704144
2214	48-49	-0.5593895054735789
2214	50-51	-0.5584753027986409
2214	52-53	-0.4973943575080675
2214	54-55	-0.6245117756839598
2214	56-57	-0.5100361748791684
2214	58-59	-0.4667432732523089
2214	60-61	-0.6725292056534471
2214	62-63	-0.5381033622475861
2214	64-65	-0.5666166446865866
2214	66-67	-0.5792229852469184
2214	68-69	-0.5510674068033659
2214	70-71	-0.5481267549589539
2214	72-73	-0.4509463018760016
2214	74-75	-0.584798398859693
2214	76-77	-0.6907346305168289
2214	78-79	-0.635535717001769
2214	80-81	-0.8336080394141518
2214	82-83	-0.7339065827906168
2214	84-85	-0.6171756431421542
2214	86-87	-0.6489428772001027
2214	88-89	-0.7873896098497646
2214	90-91	-0.658934099806082
2214	92-93	-0.5737839225305663
2214	94-95	-0.6253928844249828
2214	96-97	-0.6706498418724891
2214	98-99	-0.6182121730922319
2214	100-101	-0.5993726657270528
2214	102-103	-0.6787994057959814
2214	104-105	-0.7275401192615512
2214	106-107	-0.7811787308174871
2214	108-109	-0.7333302225804488
2214	110-111	-0.7677236045901132
2214	112-113	-0.9542316951013667
2214	114-115	-1.0012754636978372
2214	116-117	-1.108187067875324
2214	118-119	-1.0901118877763132
2214	120-121	-0.9178239906843295
2214	122-123	-0.8828425101104749
2214	124-125	-0.7293954716026434
2215	1	-0.298402301009677
2215	2	-0.36881651780047875
2215	3	-0.3468311439544003
2215	4	-0.17890480431515599
2215	5	-0.20423074848494593
2215	6	-0.41243801806946223
2215	7	-0.332656102575946
2215	8	-0.25183604856621145
2215	9	-0.10002885791917748
2215	10-11	-0.3940398362671331
2215	12-13	-0.41238805547060764
2215	14-15	-0.4436368738381944
2215	16-17	-0.25789139680729534
2215	18-19	-0.28688530470025597
2215	20-21	-0.39937889508971125
2215	22-23	-0.2919562264680522
2215	24-25	-0.46092586936400437
2215	26-27	-0.5494110376010823
2215	28-29	-0.5761143757022325
2215	30-31	-0.47996981007802475
2215	32-33	-0.5601539726694043
2215	34-35	-0.5409001083738758
2215	36-37	-0.47489309693298765
2215	38-39	-0.4898106873039083
2215	40-41	-0.5105619448397007
2215	42-43	-0.5095619184950024
2215	44-45	-0.6065531548108503
2215	46-47	-0.4309979514388935
2215	48-49	-0.5907738782929783
2215	50-51	-0.6277028014872066
2215	52-53	-0.6230184575884756
2215	54-55	-0.5251183325608473
2215	56-57	-0.6490590504292086
2215	58-59	-0.5848181367204219
2215	60-61	-0.4660055944206789
2215	62-63	-0.3986622055476552
2215	64-65	-0.5144550169135051
2215	66-67	-0.5501116014055647
2215	68-69	-0.6412502990059146
2215	70-71	-0.6255756926724594
2215	72-73	-0.5709398455834318
2215	74-75	-0.6918985491456482
2215	76-77	-0.7423141093483565
2215	78-79	-0.7726753431863642
2215	80-81	-0.6816484660412421
2215	82-83	-0.7587603566215684
2215	84-85	-0.6771773359505744
2215	86-87	-0.7175375411136606
2215	88-89	-0.8107774312742251
2215	90-91	-0.8327348385398032
2215	92-93	-0.6451520365156256
2215	94-95	-0.45539604367790076
2215	96-97	-0.5993201113341264
2215	98-99	-0.6966167602093698
2215	100-101	-0.6605283224136969
2215	102-103	-0.6478028042072985
2215	104-105	-0.6809485201185872
2215	106-107	-0.6844116012202051
2215	108-109	-0.5852498378011575
2215	110-111	-0.7229901579435989
2215	112-113	-0.7080707779141413
2215	114-115	-0.6534579563188672
2215	116-117	-0.6738188249612556
2215	118-119	-0.4317452313780379
2215	120-121	-0.47036453196966477
2215	122-123	-0.7168473897291605
2215	124-125	-0.41144373042124016
2216	1	0.0761063583442727
2216	2	-0.14190847829165065
2216	3	-0.19807881323594856
2216	4	-0.04493993727109569
2216	5	-0.22832533785854991
2216	6	-0.261806414367193
2216	7	-0.2423082166005699
2216	8	-0.07340905254732633
2216	9	-0.270429573512736
2216	10-11	-0.19524635052021466
2216	12-13	-0.29257757857621414
2216	14-15	-0.4049458977081173
2216	16-17	-0.4967091976169655
2216	18-19	-0.4793879503806622
2216	20-21	-0.485614427837362
2216	22-23	-0.5063928225272534
2216	24-25	-0.4824876397021427
2216	26-27	-0.6860352333982291
2216	28-29	-0.7781036753948314
2216	30-31	-0.5254041216131853
2216	32-33	-0.25745611357078246
2216	34-35	-0.1429341326637399
2216	36-37	-0.36432885309365304
2216	38-39	-0.6281341028353111
2216	40-41	-0.7601556439208572
2216	42-43	-0.8574720409522101
2216	44-45	-0.803857395458575
2216	46-47	-0.9119249054628469
2216	48-49	-1.0310471182855885
2216	50-51	-1.0245322894850588
2216	52-53	-1.071257085362241
2216	54-55	-0.9672781946495057
2216	56-57	-0.8292802797047116
2216	58-59	-1.1806390115587426
2216	60-61	-1.077765643806714
2216	62-63	-1.067688112218132
2216	64-65	-0.9221559416417975
2216	66-67	-0.8649874644013131
2216	68-69	-1.3020441501087028
2216	70-71	-1.2196327805762408
2216	72-73	-1.4453162209172916
2216	74-75	-1.3440487943960235
2216	76-77	-1.4747639044131162
2216	78-79	-1.8574347542495069
2216	80-81	-1.8945795439670299
2216	82-83	-2.113737175421857
2216	84-85	-2.0914194954977
2216	86-87	-2.134717770741922
2216	88-89	-2.0891938235740355
2216	90-91	-2.1729745875781177
2216	92-93	-2.1107770890092823
2216	94-95	-1.9234274054893987
2216	96-97	-2.1143558700144816
2216	98-99	-1.9069068821626374
2216	100-101	-1.7331218031212288
2216	102-103	-1.778302039899625
2216	104-105	-2.348137673668834
2216	106-107	-2.3218016794987513
2216	108-109	-2.5103566099030843
2216	110-111	-2.6123057036256796
2216	112-113	-2.8979345673683774
2216	114-115	-2.6883599401592164
2216	116-117	-3.0801076702687418
2216	118-119	-2.835023565355293
2216	120-121	-2.113086391169073
2216	122-123	-1.5969907184150536
2216	124-125	-1.5992250964322245
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36974.0
3	4289.0
4	2121.0
5	4216.0
6	11523.0
7	10334.0
8	6396.0
9	6235.0
10	4755.0
11	3149.0
12	2527.0
13	1935.0
14	2110.0
15	2044.0
16	1815.0
17	1794.0
18	1972.0
19	2267.0
20	2618.0
21	3030.0
22	3608.0
23	4135.0
24	4662.0
25	5265.0
26	5897.0
27	6609.0
28	7792.0
29	9706.0
30	12338.0
31	16608.0
32	22468.0
33	31092.0
34	46864.0
35	76940.0
36	134061.0
37	45179.0
38	3590.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	56.40926929750461	18.741299314527385	11.325350444909494	13.524080943058516
2	20.331864776127016	23.858952915067835	34.82703457122138	20.982147737583766
3	30.60158868969538	24.541065442355343	24.692829033668563	20.164516834280715
4	16.326802053763714	23.126341450971672	28.1078845432052	32.43897195205941
5	15.874455964307252	36.41145039141421	24.869422324728962	22.84467131954958
6	32.234446247989226	36.61013737910329	9.250362986160072	21.905053386747408
7	29.328242105378216	41.37448580662321	11.213332410305364	18.083939677693206
8	35.32574141445792	29.882659855679456	9.948134235230462	24.84346449463216
9	36.29060025249627	17.787505989043918	14.482646802169356	31.439246956290457
10-11	30.912757619976716	27.99028636283313	14.485197269542502	26.611758747647652
12-13	33.11599707789309	29.710576606020194	16.589150676147117	20.58427563993959
14-15	35.95111482750642	23.30216297850867	20.285324428558678	20.461397765426227
16-17	30.29973456921695	24.347399160896234	21.317443921598635	24.03542234828818
18-19	29.501984810145032	25.025550217791697	20.930137233362302	24.542327738700976
20-21	38.55505092736816	22.591129110123717	17.496752708505262	21.357067254002867
22-23	34.37720319391386	25.52188938623003	16.570933052053217	23.5299743678029
24-25	27.490850198298837	27.81812981514577	19.047892311980455	25.643127674574938
26-27	24.700181266680634	27.524935554685154	21.19544920433947	26.579433974294748
28-29	26.10670055431817	26.52874756887984	22.56853823064555	24.79601364615644
30-31	26.87682517821391	27.748538491107066	19.677675576906612	25.696960753772412
32-33	24.16276354070455	27.936817636117535	20.809081121258327	27.091337701919592
34-35	23.63818705492067	28.75342263612664	21.107394715795955	26.500995593156734
36-37	23.830656253472736	29.344402361732786	19.78251800356701	27.042423381227465
38-39	24.527116022582568	26.950879820155617	20.75142234100113	27.770581816260687
40-41	23.49189953344665	27.004530723112154	22.424164585303114	27.079405158138087
42-43	23.16225162546751	27.36378227024388	23.663327376170255	25.810638728118356
44-45	22.99310098575564	26.915082188811102	24.02230565894057	26.06951116649269
46-47	21.033340073854248	27.11201470526617	23.69511713021411	28.15952809066547
48-49	20.557586820642026	27.48921061213039	24.745909687950316	27.207292879277272
50-51	20.988433630462783	27.33845977275336	23.868093470985702	27.805013125798162
52-53	21.424017022547954	25.979698279709755	22.920230489380035	29.676054208362252
54-55	20.27220773921107	25.84898682683602	23.081729726972465	30.797075706980447
56-57	21.414218517155568	25.145285816825098	24.00495155924929	29.435544106770045
58-59	22.765927213304696	23.83138495843649	23.443167388995462	29.959520439263354
60-61	22.509969594785385	23.891685294187305	23.170449556309766	30.427895554717544
62-63	22.846722376161146	23.195589877559346	23.526057578622712	30.43163016765679
64-65	23.075507687038517	22.379037767506937	23.70366052055798	30.84179402489657
66-67	24.127239173721442	23.293782871425474	22.90328809897271	29.67568985588038
68-69	23.932547320630693	23.45139313357813	23.089844294424093	29.526215251367084
70-71	23.719367869813105	22.833179364116244	24.052907102809527	29.394545663261123
72-73	24.75458560788168	21.95517185155902	24.563937998081684	28.726304542477614
74-75	25.132065709009982	20.92636841390883	24.93803228242522	29.003533594655973
76-77	24.91326094223803	20.57352985381201	25.684052128146995	28.829157075802968
78-79	25.470336937412895	21.234253026424856	26.321197270980022	26.97421276518222
80-81	26.420618090928304	22.028324916745003	25.361621814631018	26.189435177695675
82-83	25.552336093609522	21.48943786737011	26.03218874933164	26.926037289688725
84-85	25.47947227301426	21.492456813102613	26.4812825649842	26.546788348898932
86-87	26.094703176964302	21.367195932990164	26.187797695227495	26.350303194818043
88-89	26.09071561585528	20.949538459295862	25.95906809957472	27.00067782527414
90-91	26.79885374709201	21.136451826225727	25.530816022036042	26.53387840464622
92-93	27.207059310121156	21.86684352597454	25.24645362772634	25.67964353617796
94-95	26.475046779885837	22.531962459483243	24.68830111123865	26.30468964939227
96-97	25.70528317905311	23.2226499518121	24.086206236916848	26.98586063221794
98-99	25.35800789024705	24.30636967565455	23.85729419727075	26.478328236827654
100-101	25.01794445780411	25.721604034132728	23.61955198973978	25.640899518323383
102-103	24.508898398488665	27.118208697458098	23.63171979836734	24.741173105685903
104-105	23.87662991250962	28.79713253813187	23.334380849581223	23.99185669977729
106-107	23.1526950007697	29.64450747794055	23.21891660723118	23.983880914058563
108-109	22.866488498303028	30.994372575917396	23.28348991381242	22.85564901196716
110-111	22.97916450332381	33.04913839746848	21.743371972914037	22.22832512629368
112-113	22.94703724080639	33.82223809194154	21.41810921200771	21.812615455244355
114-115	22.68253379766311	34.25267106522874	21.863428382476318	21.201366754631835
116-117	22.699888325964306	34.87481759603876	21.290390749819192	21.134903328177746
118-119	22.926211202771068	35.08108108108108	21.22583291554624	20.766874800601613
120-121	23.256116878586074	34.51294061064489	21.44945041231601	20.78149209845303
122-123	24.060175717467448	35.05659122687958	20.784215488460855	20.099017567192117
124-125	25.05382372115021	36.04874539142948	19.56818675689939	19.329244130520927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	10.5
2	11.0
3	10.5
4	11.0
5	8.0
6	7.5
7	11.5
8	11.5
9	12.5
10	20.0
11	26.5
12	26.5
13	28.0
14	35.0
15	39.0
16	51.0
17	74.5
18	101.5
19	148.5
20	226.0
21	310.5
22	412.5
23	579.5
24	805.5
25	1080.5
26	1369.0
27	1682.5
28	2035.0
29	2380.0
30	2752.0
31	3117.5
32	3306.5
33	3490.0
34	3735.5
35	3874.0
36	3931.5
37	3913.0
38	3754.0
39	3584.0
40	3407.0
41	3154.5
42	2996.0
43	2845.5
44	2824.0
45	3315.5
46	4313.0
47	5466.5
48	7150.5
49	10690.0
50	17999.5
51	28984.5
52	42109.0
53	52402.5
54	55276.0
55	51739.0
56	44876.0
57	36971.5
58	28302.0
59	21226.5
60	16444.5
61	12386.0
62	9199.5
63	7188.0
64	5902.5
65	4783.5
66	3999.5
67	3411.0
68	2980.5
69	2422.5
70	1773.0
71	1371.5
72	1080.5
73	881.5
74	618.5
75	400.0
76	269.0
77	175.0
78	120.0
79	92.0
80	75.5
81	60.5
82	50.0
83	46.5
84	36.0
85	24.5
86	20.5
87	18.0
88	13.0
89	8.0
90	6.0
91	4.0
92	3.0
93	4.0
94	3.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15120627997937766
2	0.014756275516059748
3	0.00710487339662136
4	0.013298865588547672
5	0.0021861148912681104
6	0.0
7	1.821762409390092E-4
8	3.643524818780184E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	7.287049637560368E-4
28-29	0.008471195203663929
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.821762409390092E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0014574099275120737
66-67	0.0
68-69	0.005556375348639781
70-71	0.019675034021412997
72-73	9.10881204695046E-5
74-75	0.00810684272178591
76-77	4.554406023475231E-4
78-79	7.287049637560368E-4
80-81	5.465287228170276E-4
82-83	9.10881204695046E-5
84-85	0.020494827105638536
86-87	0.002641555493615634
88-89	0.019128505298595968
90-91	0.0
92-93	0.015211716118407268
94-95	0.013116689347608663
96-97	0.004736582264414239
98-99	0.0024593792526766247
100-101	5.465287228170276E-4
102-103	0.0
104-105	2.732643614085138E-4
106-107	8.197930842255414E-4
108-109	0.0
110-111	0.0
112-113	9.108812046950462E-4
114-115	0.005009846625822754
116-117	0.0
118-119	0.07177743892996963
120-121	0.001366321807042569
122-123	0.004827670384883745
124-125	0.236464760738834
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	548919.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.41474044539014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72117207902711	49.7427170885924
2	7.786440289067317	9.252586575369264
3	2.8052011878071386	5.000109014119838
4	1.469379557446063	3.492112200856803
5	0.9188682811718939	2.729716021466492
6	0.6304074394358951	2.2473296633356026
7	0.4337953768811574	1.8041687806662912
8	0.3627967545037316	1.7244380002615312
9	0.2691403557501252	1.4391813942246288
>10	1.5214418137804364	15.967873008597069
>50	0.05138328460418083	2.0385539740952434
>100	0.026915051899975048	2.6558624174913903
>500	0.0012234114499988277	0.4272276800220416
>1k	0.0018351171749982419	1.4781241809014407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT	1697	0.30915308087349863	No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA	1592	0.2900245755749027	No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT	1503	0.27381089013133086	No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA	1137	0.2071343859476535	No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC	1118	0.2036730373698123	No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC	1087	0.19802557390070302	No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA	641	0.11677497044190492	No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT	622	0.11331362186406374	No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA	553	0.1007434612392721	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.821762409390092E-4	0.0	0.0	0.0	0.0
2	1.821762409390092E-4	0.0	0.0	0.0	0.0
3	1.821762409390092E-4	0.0	0.0	0.0	0.0
4	1.821762409390092E-4	0.0	0.0	0.0	0.0
5	9.108812046950461E-4	0.0	0.0	0.0	0.0
6	0.0012752336865730644	0.0	0.0	0.0	0.0
7	0.0014574099275120737	0.0	0.0	0.0	0.0
8	0.001639586168451083	0.0	0.0	0.0	0.0
9	0.001639586168451083	0.0	0.0	1.821762409390092E-4	0.0
10-11	0.004098965421127707	0.0	0.0	0.0010930574456340552	0.0
12-13	0.006285080312395818	0.0	0.0	0.0014574099275120737	0.0
14-15	0.007560313998968882	0.0	0.0	0.0014574099275120737	0.0
16-17	0.009473164528828478	0.0	0.0	0.001639586168451083	0.0
18-19	0.01420974679324272	0.0	0.0	0.0017306742889205875	0.0
20-21	0.019037417178126462	0.0	0.0	0.004098965421127707	0.0
22-23	0.02095026770798606	0.0	0.0	0.019219593419065473	0.0
24-25	0.023045294478784666	0.0	0.0	0.043813385945831715	0.0
26-27	0.028510581706954943	0.0	0.0	0.11504429615298431	0.0
28-29	0.035433278862637294	0.0	0.0	0.35852284216797015	0.0
30-31	0.040078773006582025	0.0	0.0	0.8745370446277136	0.0
32-33	0.043540121584423205	0.0	0.0	1.5730918405083445	0.0
34-35	0.04654602955991685	0.0	0.0	2.5671364991920482	0.0
36-37	0.05228458114949565	0.0	0.0	3.9445710569318972	0.0
38-39	0.05966271890752552	0.0	0.0	5.833829763589892	0.0
40-41	0.06667650418367738	0.0	0.0	8.06621742005651	0.0
42-43	0.07496552314640229	0.0	0.0	9.761002989512114	0.0
44-45	0.07660510931485337	0.0	0.0	11.254119460248234	0.0
46-47	0.0770605499172009	0.0	0.0	13.029518016319347	0.0
48-49	0.0770605499172009	0.0	0.0	14.978712706246277	0.0
50-51	0.0770605499172009	0.0	0.0	16.920529258415176	0.0
52-53	0.0772427261581399	0.0	0.0	18.723345338747613	0.0
54-55	0.0772427261581399	0.0	0.0	20.401917222759643	0.0
56-57	0.07742490239907891	0.0	0.0	22.093059267396466	0.0
58-59	0.07742490239907891	0.0	0.0	23.770447005842392	0.0
60-61	0.07751599051954841	0.0	0.0	25.58519562995633	0.0
62-63	0.07760707864001792	0.0	0.0	27.307034371191378	0.0
64-65	0.07778925488095693	0.0	0.0	28.984331021516837	0.0
66-67	0.07788034300142643	0.0	0.0	30.76747206782786	0.0
68-69	0.07824469548330446	0.0	0.0	32.630952836392986	0.0
70-71	0.07851795984471296	0.0	0.0	34.65884766240556	0.0
72-73	0.07888231232659099	0.0	0.0	36.66196651965044	0.0
74-75	0.07888231232659099	0.0	0.0	38.44428777287723	0.0
76-77	0.07888231232659099	0.0	0.0	40.3184258515373	0.0
78-79	0.0789734004470605	0.0	0.0	42.17990268145209	0.0
80-81	0.07906448856753	0.0	0.0	43.99082560450631	0.0
82-83	0.07906448856753	0.0	0.0	45.92216702282121	0.0
84-85	0.07906448856753	0.0	0.0	47.99232673673165	0.0
86-87	0.07906448856753	0.0	0.0	49.647944414385364	0.0
88-89	0.07906448856753	0.0	0.0	51.166656646973415	0.0
90-91	0.07906448856753	0.0	0.0	53.08533681654306	0.0
92-93	0.07906448856753	0.0	0.0	54.702515307358645	0.0
94-95	0.07906448856753	0.0	0.0	56.156463886292876	0.0
96-97	0.07906448856753	0.0	0.0	57.637283460765616	0.0
98-99	0.07906448856753	0.0	0.0	58.91953093261483	0.0
100-101	0.07906448856753	0.0	0.0	60.175180673286945	0.0
102-103	0.07924666480846901	0.0	0.0	61.383646767555874	0.0
104-105	0.07924666480846901	0.0	0.0	62.524161123954535	0.0
106-107	0.07924666480846901	0.0	0.0	63.54516786629721	0.0
108-109	0.07924666480846901	0.0	0.0	64.52983044857257	0.0
110-111	0.07924666480846901	0.0	0.0	65.52924930636397	0.0
112-113	0.07924666480846901	0.0	0.0	66.51628017977151	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAT	25	0.0020474063	71.47782	1
TCTACAA	30	0.004238508	59.488888	2
TATGGGA	70	1.3418685E-8	59.488884	4
CTATGGG	65	5.4832526E-7	54.912815	3
GTCCAGT	95	2.6029738E-9	50.159878	1
TCGTAGG	60	2.1942978E-5	49.574074	2
GGAAACA	525	0.0	45.382748	1
TATGGGT	80	2.2946406E-6	44.616665	4
GGTATTC	35	5.4563574E-5	42.493996	28-29
TCATAGG	170	7.2759576E-12	38.492805	2
TAACAAC	255	0.0	37.374027	1
GTTGTAG	80	1.1890049E-4	37.228035	1
TTAGGGA	80	1.1979707E-4	37.180553	4
TTGTAGG	80	1.1979707E-4	37.180553	2
TGCTAGG	80	1.1979707E-4	37.180553	2
AGTAATT	80	1.1986133E-4	37.17717	7
GTATAGG	210	0.0	36.87348	1
TGATAGA	65	0.0019397968	36.608543	3
ATAAGGG	115	6.6587927E-7	36.210625	3
GTCTATG	100	1.0532822E-5	35.73891	1
>>END_MODULE