FastQCFastQC Report
Thu 26 May 2016
SRR935987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548919
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT91681.6701917769288366No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT50320.9167108444050944RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT29960.5458000178532716RNA PCR Primer, Index 26 (100% over 24bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA16740.30496302733190145No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT15850.2887493418883296No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT14540.26488425432531937No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC11500.20950267707986062No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC11370.2071343859476535No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA11210.20421956609262934No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC9040.16468732180886433RNA PCR Primer, Index 26 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG7030.12806989738012348RNA PCR Primer, Index 26 (96% over 27bp)
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA6900.12570160624791638No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA5760.1049335147808693No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT5750.10475133853993031RNA PCR Primer, Index 26 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA950.081.705151
TATATTT250.002045024771.49883
CTATACA851.2732926E-1163.219821
CTTAACA401.740917E-459.7076071
TATTTGG300.004212245359.5823252
TATCGAG507.4501677E-659.4954957
TCTAGAC150.004237773459.49548792-93
TAGTGTT300.004236646559.4954879
GTAGTGT300.004236646559.4954878
GCTTATA551.2991191E-554.1657562
TATTAGG350.00775160351.0705682
TATATCG602.1869577E-549.6021655
GGCTTAT653.446124E-545.928931
TTATAGG653.4891542E-545.832562
CATCCGA402.294999E-644.6216226-27
GAGATGT1109.498763E-943.3326033
GTAAAAT803.6379788E-1240.90315214-15
GTTATAG758.016785E-539.805071
TCTACGG905.1018596E-639.7215542
TGTTAGG758.116642E-539.7215542
Produced by FastQC (version 0.11.5)