##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935987_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 548919 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33554130937351 33.0 31.0 34.0 31.0 34.0 2 32.44346980155542 34.0 31.0 34.0 31.0 34.0 3 32.43274872977616 34.0 31.0 34.0 30.0 34.0 4 35.921465644293605 37.0 35.0 37.0 35.0 37.0 5 35.91705880102529 37.0 35.0 37.0 35.0 37.0 6 36.003849383971044 37.0 35.0 37.0 35.0 37.0 7 35.98181152410465 37.0 35.0 37.0 35.0 37.0 8 36.00733988074743 37.0 35.0 37.0 35.0 37.0 9 37.68327749631548 39.0 37.0 39.0 35.0 39.0 10-11 37.61509894902527 39.0 37.0 39.0 35.0 39.0 12-13 37.57972761008455 39.0 37.0 39.0 35.0 39.0 14-15 38.68329753570199 40.0 38.0 41.0 34.0 41.0 16-17 38.643073021702655 40.0 38.0 41.0 34.5 41.0 18-19 38.73765072806735 40.0 38.0 41.0 34.5 41.0 20-21 38.83525073826922 40.0 38.0 41.0 35.0 41.0 22-23 38.66894204791599 40.0 38.0 41.0 34.0 41.0 24-25 38.348368338498034 40.0 38.0 41.0 34.0 41.0 26-27 38.189697387046174 40.0 37.5 41.0 33.0 41.0 28-29 38.09293720931504 40.0 37.0 41.0 33.0 41.0 30-31 37.89798585948018 40.0 37.0 41.0 33.0 41.0 32-33 37.494110242130446 40.0 35.5 41.0 33.0 41.0 34-35 37.219946840972895 40.0 35.0 41.0 33.0 41.0 36-37 37.06237987754113 40.0 35.0 41.0 32.5 41.0 38-39 36.593171305784644 39.0 35.0 41.0 31.5 41.0 40-41 36.25330877597605 39.0 35.0 41.0 31.0 41.0 42-43 35.85070201614445 39.0 35.0 41.0 30.0 41.0 44-45 35.30032026583157 38.5 35.0 41.0 28.5 41.0 46-47 34.637564923057866 38.0 35.0 40.5 26.0 41.0 48-49 33.779599540187164 37.0 34.5 40.0 20.5 41.0 50-51 32.914166753200384 36.5 34.0 39.5 4.5 41.0 52-53 32.400020768091466 36.0 33.5 39.0 2.0 41.0 54-55 31.898117026373654 35.0 33.0 39.0 2.0 41.0 56-57 31.55244216359791 35.0 33.0 37.5 2.0 40.0 58-59 31.258517194704503 35.0 33.0 37.0 2.0 39.5 60-61 30.854338253913603 35.0 33.0 36.0 2.0 39.0 62-63 30.54126929474112 35.0 32.0 35.5 2.0 38.5 64-65 30.265367932245013 35.0 32.0 35.0 2.0 37.0 66-67 29.823115067979064 35.0 32.0 35.0 2.0 37.0 68-69 29.35001703347853 35.0 31.0 35.0 2.0 36.0 70-71 28.882664837617206 35.0 31.0 35.0 2.0 36.0 72-73 28.599370763263796 35.0 30.0 35.0 2.0 35.0 74-75 28.501062087484677 35.0 30.0 35.0 2.0 35.0 76-77 28.400510822179594 35.0 30.0 35.0 2.0 35.0 78-79 28.453527751817663 35.0 30.5 35.0 2.0 35.0 80-81 28.42970638655248 35.0 31.0 35.0 2.0 35.0 82-83 28.347894680271587 35.0 30.0 35.0 2.0 35.0 84-85 28.302097395061928 35.0 30.0 35.0 2.0 35.0 86-87 28.274002175184314 35.0 30.0 35.0 2.0 35.0 88-89 28.199411024213045 35.0 30.0 35.0 2.0 35.0 90-91 28.09992275727384 35.0 29.5 35.0 2.0 35.0 92-93 28.02898150728978 35.0 29.0 35.0 2.0 35.0 94-95 27.987413443513525 35.0 29.0 35.0 2.0 35.0 96-97 27.953066845928088 35.0 29.0 35.0 2.0 35.0 98-99 27.881089013133085 35.0 29.0 35.0 2.0 35.0 100-101 27.783490824693622 35.0 29.0 35.0 2.0 35.0 102-103 27.071733716632146 33.5 27.0 35.0 2.0 35.0 104-105 27.472885799179842 34.0 29.0 35.0 2.0 35.0 106-107 27.33322220582636 35.0 29.0 35.0 2.0 35.0 108-109 27.06084686447363 35.0 27.0 35.0 2.0 35.0 110-111 26.680842710855337 34.0 25.0 35.0 2.0 35.0 112-113 26.185899012422595 34.0 22.0 35.0 2.0 35.0 114-115 25.540055090095258 34.0 8.5 35.0 2.0 35.0 116-117 24.89136102047843 34.0 2.0 35.0 2.0 35.0 118-119 24.348084143562165 34.0 2.0 35.0 2.0 35.0 120-121 23.754175024001718 34.0 2.0 35.0 2.0 35.0 122-123 23.07721904324682 33.5 2.0 35.0 2.0 35.0 124-125 20.915152326663858 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06275908182052348 1101 2 0.13401599603584913 1101 3 0.23441939294361447 1101 4 0.18682043777183566 1101 5 0.23795148450584236 1101 6 0.16495278984592687 1101 7 0.30701827415467875 1101 8 0.18418014720003129 1101 9 0.331994026475094 1101 10-11 0.12813559009479292 1101 12-13 0.26110927057860067 1101 14-15 0.3579387981413049 1101 16-17 0.21442836977286817 1101 18-19 0.34724599458596117 1101 20-21 0.33368279495385167 1101 22-23 0.4189524767474495 1101 24-25 0.41895377668601697 1101 26-27 0.41812226316766044 1101 28-29 0.4962481541586854 1101 30-31 0.5103159785884159 1101 32-33 0.6164523796340262 1101 34-35 0.3170441334875136 1101 36-37 0.2470341498453763 1101 38-39 0.2087993010988285 1101 40-41 0.3475211444205826 1101 42-43 0.20364514309394366 1101 44-45 0.25058646202685964 1101 46-47 0.4102595863666352 1101 48-49 0.09638067762219293 1101 50-51 0.2614980036764223 1101 52-53 0.24702201013106162 1101 54-55 0.32167106962188186 1101 56-57 0.07777527429050934 1101 58-59 0.33751890778387406 1101 60-61 0.1669072285601949 1101 62-63 0.19575066201907987 1101 64-65 0.23285745840206928 1101 66-67 -0.007104477011203869 1101 68-69 0.3037078876780548 1101 70-71 0.20371048927424695 1101 72-73 0.23155885436046475 1101 74-75 0.0982843313504489 1101 76-77 0.16232613642531746 1101 78-79 0.2023196480376832 1101 80-81 0.27283638017547673 1101 82-83 0.17219945637276268 1101 84-85 0.19598817889941955 1101 86-87 0.15606176632494595 1101 88-89 -0.0948807161332077 1101 90-91 0.03189117535831798 1101 92-93 0.03222155790998116 1101 94-95 0.1761011613568293 1101 96-97 -5.685910080615031E-4 1101 98-99 0.03288348655083695 1101 100-101 -0.03890162834564137 1101 102-103 0.07640528006860237 1101 104-105 0.20907488445341116 1101 106-107 0.2575893195255503 1101 108-109 0.06792595465798712 1101 110-111 -0.015997453761027458 1101 112-113 0.02690019488687767 1101 114-115 -0.2727471643124133 1101 116-117 -0.3944016069386258 1101 118-119 -0.6234190404905142 1101 120-121 -0.45221017436425015 1101 122-123 -0.5596860283275085 1101 124-125 -0.937905906439056 1102 1 0.16897031118413963 1102 2 0.18020582103535077 1102 3 0.23033670867780387 1102 4 0.23531717757008153 1102 5 0.29167643842699675 1102 6 0.30428727267288735 1102 7 0.3599861477896553 1102 8 0.28601735511964677 1102 9 0.4045310381880398 1102 10-11 0.41231617950757027 1102 12-13 0.31621262591086463 1102 14-15 0.440775669140379 1102 16-17 0.34808772328179316 1102 18-19 0.39582200980684235 1102 20-21 0.47692159234569687 1102 22-23 0.5087764853091912 1102 24-25 0.4591127239083974 1102 26-27 0.6289205666736137 1102 28-29 0.577925622101688 1102 30-31 0.49809567218890294 1102 32-33 0.6272368099410244 1102 34-35 0.5330419137756834 1102 36-37 0.7683909487038179 1102 38-39 0.7329287578639949 1102 40-41 0.5258710264983932 1102 42-43 0.37061702013775033 1102 44-45 0.3322817669399498 1102 46-47 0.3296969290544709 1102 48-49 0.14692965992395557 1102 50-51 -0.2626899187129368 1102 52-53 -0.30867491992819396 1102 54-55 -0.2199108714368414 1102 56-57 -0.32657298264113166 1102 58-59 -0.32306634302374704 1102 60-61 -0.34314390408740536 1102 62-63 -0.25053132065923833 1102 64-65 -0.19837408528859513 1102 66-67 -0.2123673741315244 1102 68-69 0.04674255085660306 1102 70-71 0.062321782058202047 1102 72-73 0.05183948935659899 1102 74-75 0.0010599620855273884 1102 76-77 0.013945336932678742 1102 78-79 -0.0015717842710145646 1102 80-81 0.008421135511703426 1102 82-83 0.08852622966487544 1102 84-85 0.04863895084384495 1102 86-87 -0.03835526203583939 1102 88-89 0.034215345860172164 1102 90-91 0.08957197286906649 1102 92-93 0.033592031778820086 1102 94-95 -0.0258955883508456 1102 96-97 0.12045426013251515 1102 98-99 -0.01801926232444373 1102 100-101 0.03088452620179183 1102 102-103 0.23482135712430718 1102 104-105 0.06903088659384693 1102 106-107 0.29634707365282864 1102 108-109 0.15468596813481028 1102 110-111 0.10594301039657594 1102 112-113 0.10320972577456189 1102 114-115 0.10596837565230999 1102 116-117 0.06872640305007849 1102 118-119 0.1087558807295359 1102 120-121 0.4979008112274421 1102 122-123 0.18084452243849114 1102 124-125 0.20293877318914255 1103 1 0.045580392339672926 1103 2 0.12752173198762762 1103 3 0.06761985959731476 1103 4 0.10235611733629213 1103 5 -0.01509148652274206 1103 6 0.1656527704020192 1103 7 0.1464019023872325 1103 8 0.1966048020707305 1103 9 0.2847745047951449 1103 10-11 0.33843113744070763 1103 12-13 0.23818976834255068 1103 14-15 0.2539187709198458 1103 16-17 0.36896852143532044 1103 18-19 0.39292944782337 1103 20-21 0.36139035863264013 1103 22-23 0.3695649597340278 1103 24-25 0.3242716845219107 1103 26-27 0.1512643981083528 1103 28-29 0.2457426126459552 1103 30-31 0.3925636939296595 1103 32-33 0.367055140668441 1103 34-35 0.5242432390679141 1103 36-37 0.6024930259877053 1103 38-39 0.6294535884797341 1103 40-41 0.45997496514778646 1103 42-43 0.43302488254561666 1103 44-45 0.3423033587797235 1103 46-47 0.22669454650663567 1103 48-49 0.35775536165875366 1103 50-51 0.34464013861712317 1103 52-53 0.5124701921260097 1103 54-55 0.5671600838159314 1103 56-57 0.5277509694100857 1103 58-59 0.5930847254000469 1103 60-61 0.7461848875141115 1103 62-63 0.9169785445779013 1103 64-65 0.8044644970954415 1103 66-67 0.7280987117891087 1103 68-69 0.7631430422570951 1103 70-71 0.7401678099042321 1103 72-73 0.7533360272167755 1103 74-75 0.7322285273450007 1103 76-77 0.7273173866683642 1103 78-79 0.8682685105692798 1103 80-81 0.8336277470819553 1103 82-83 0.8607956064797087 1103 84-85 0.6465374691969075 1103 86-87 0.5919552137291788 1103 88-89 0.5741937540203992 1103 90-91 0.6188977862857925 1103 92-93 0.6564924114973785 1103 94-95 0.6962304633206635 1103 96-97 0.7613894101585714 1103 98-99 0.6883763895257538 1103 100-101 0.7803186710904839 1103 102-103 0.7339446539748806 1103 104-105 0.6595658430378961 1103 106-107 0.4744034358000988 1103 108-109 0.35515130950924245 1103 110-111 0.3977250817241007 1103 112-113 0.35905069070643236 1103 114-115 0.5714828570758783 1103 116-117 0.8245561997889617 1103 118-119 0.7629216167134523 1103 120-121 0.7290507628319425 1103 122-123 0.6074732172490016 1103 124-125 0.5182578112354541 1104 1 0.02341787314941257 1104 2 0.07201814385523875 1104 3 0.10109302893574323 1104 4 0.07508631120255416 1104 5 0.2485309205606967 1104 6 0.27501047685351665 1104 7 0.20914423520882508 1104 8 -0.020750333778458696 1104 9 0.10520900799054544 1104 10-11 0.13755463345984253 1104 12-13 0.20696141317099404 1104 14-15 0.1601569443640969 1104 16-17 0.19140773740161876 1104 18-19 0.3089796459739631 1104 20-21 0.2068436058031935 1104 22-23 0.20815255657790033 1104 24-25 0.16837358034110395 1104 26-27 0.23923076606151028 1104 28-29 0.2533098231854183 1104 30-31 0.2372064873174935 1104 32-33 0.10609437805642585 1104 34-35 0.3852459524814691 1104 36-37 0.39764190670462085 1104 38-39 0.4862576514594892 1104 40-41 0.5225066513914953 1104 42-43 0.3369914647156307 1104 44-45 0.35312488043347656 1104 46-47 0.26365855807434 1104 48-49 0.4859409448641543 1104 50-51 0.34786410234306686 1104 52-53 0.13028232394078287 1104 54-55 0.07876885242601972 1104 56-57 0.08753264672809635 1104 58-59 -0.0524015468695751 1104 60-61 0.053176390245376126 1104 62-63 -0.17215084240485368 1104 64-65 -0.033520614832870166 1104 66-67 0.22665810341322867 1104 68-69 0.21571096685276103 1104 70-71 0.23700454095496326 1104 72-73 0.06055156507960646 1104 74-75 0.03597285952394458 1104 76-77 0.10228793185027385 1104 78-79 0.10846184128440584 1104 80-81 0.07279551458525901 1104 82-83 0.13191738876397352 1104 84-85 0.17161423023800282 1104 86-87 0.20587216899356164 1104 88-89 0.2954065784696809 1104 90-91 0.23499886094036526 1104 92-93 0.21714880765575728 1104 94-95 0.3375287959994431 1104 96-97 0.30488078793000284 1104 98-99 0.3262642112973282 1104 100-101 0.12864063902935996 1104 102-103 0.10615447938814171 1104 104-105 0.19342906557350759 1104 106-107 0.20115108834035667 1104 108-109 0.3849345934536892 1104 110-111 0.33282362147917866 1104 112-113 0.21884397143762868 1104 114-115 0.49527788724475386 1104 116-117 0.5124172444531787 1104 118-119 0.41174342398968733 1104 120-121 0.35698553379841513 1104 122-123 0.30061827776526684 1104 124-125 0.19420304312520287 1105 1 0.10089593806442565 1105 2 0.13168292643015178 1105 3 0.1323291980918242 1105 4 0.17832902118643545 1105 5 0.0742428606121166 1105 6 0.09411064003636227 1105 7 0.23184117797995896 1105 8 0.21739694971709866 1105 9 0.36683367393871436 1105 10-11 0.23303264431842763 1105 12-13 0.2299624233505 1105 14-15 0.3728774354667479 1105 16-17 0.4207311536327296 1105 18-19 0.3471493966724779 1105 20-21 0.30112561818886974 1105 22-23 0.3124003782727698 1105 24-25 0.302936922650062 1105 26-27 0.2505800820787698 1105 28-29 0.3582814942385397 1105 30-31 0.34544265985209677 1105 32-33 0.23192000553331127 1105 34-35 0.18132314506087255 1105 36-37 0.1711302693691934 1105 38-39 0.33422479190822685 1105 40-41 0.2751647073196253 1105 42-43 0.2634907704283833 1105 44-45 0.09400032428742833 1105 46-47 0.08815338386060745 1105 48-49 0.01655124193801072 1105 50-51 0.008661704940479353 1105 52-53 0.1441249927066437 1105 54-55 0.1205367341235295 1105 56-57 0.00309312742488288 1105 58-59 0.13256941469292371 1105 60-61 -0.06704003817884541 1105 62-63 0.004365355941693849 1105 64-65 0.11224254368422848 1105 66-67 0.06995949840692717 1105 68-69 0.05575208352342642 1105 70-71 0.027058664953653278 1105 72-73 0.17323303419818004 1105 74-75 0.17530414772241443 1105 76-77 0.14768097276084902 1105 78-79 0.07416641614749153 1105 80-81 0.05459961408962499 1105 82-83 0.12294740021717843 1105 84-85 0.3079479205689992 1105 86-87 0.29632377666552046 1105 88-89 0.29999039465079846 1105 90-91 0.29520420779681444 1105 92-93 0.2660538271329642 1105 94-95 0.3385457325540919 1105 96-97 0.2012318101032733 1105 98-99 0.04135558355353908 1105 100-101 0.14127463984696575 1105 102-103 0.23981491042058067 1105 104-105 0.2569892895022612 1105 106-107 0.170447881780607 1105 108-109 0.13534761835005327 1105 110-111 0.2653738686184113 1105 112-113 0.09910391528651274 1105 114-115 0.03681602912260473 1105 116-117 0.320332794798297 1105 118-119 0.34447273304717996 1105 120-121 0.478979911929887 1105 122-123 0.572559275173937 1105 124-125 0.5333023954616216 1106 1 0.1443544241033976 1106 2 0.11565213042729994 1106 3 0.20249029704507393 1106 4 0.14054920733350684 1106 5 0.1670065909361611 1106 6 0.14398935440870986 1106 7 0.16920769787343914 1106 8 0.14055854963272907 1106 9 0.2089316516327031 1106 10-11 0.1379470193019472 1106 12-13 0.21764435179197505 1106 14-15 0.14266215347765865 1106 16-17 0.15456687752336506 1106 18-19 0.15530058184398854 1106 20-21 0.0902633037427023 1106 22-23 0.07386978001822797 1106 24-25 0.2702211133321626 1106 26-27 0.29614458757617257 1106 28-29 0.42128245661363195 1106 30-31 0.40626179925146744 1106 32-33 0.2325643354302187 1106 34-35 0.26780342296315496 1106 36-37 0.3478991279659809 1106 38-39 0.4376801139743023 1106 40-41 0.5200578573183066 1106 42-43 0.6210856236991376 1106 44-45 0.543971650783071 1106 46-47 0.4523876365038433 1106 48-49 0.5751836146540157 1106 50-51 0.44609249303339027 1106 52-53 0.18436347716362178 1106 54-55 0.16766509914053174 1106 56-57 0.18391912209886385 1106 58-59 0.32233033699102265 1106 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-0.8226905950203616 2216 28-29 -1.0596169842390708 2216 30-31 -0.8916151883424419 2216 32-33 -0.9126774520938028 2216 34-35 -1.0167009890585064 2216 36-37 -0.7591251599948876 2216 38-39 -1.237381042761676 2216 40-41 -0.7809807151087398 2216 42-43 -0.9312416085217237 2216 44-45 -0.7956175070013103 2216 46-47 -1.1596602404837455 2216 48-49 -1.4341537084282834 2216 50-51 -1.3171326511217707 2216 52-53 -1.3458927258767375 2216 54-55 -1.50651146909291 2216 56-57 -1.2772736526696136 2216 58-59 -0.9806412251267496 2216 60-61 -1.1036977281524045 2216 62-63 -1.1875250055824296 2216 64-65 -1.2286464740451954 2216 66-67 -1.2831157866075955 2216 68-69 -1.093713920638006 2216 70-71 -0.9528778840948533 2216 72-73 -0.7066788605523513 2216 74-75 -0.8766554625771263 2216 76-77 -1.1871609688057525 2216 78-79 -1.0830914298796444 2216 80-81 -0.9342747952909249 2216 82-83 -1.0471808021322957 2216 84-85 -1.0671306480729044 2216 86-87 -1.32454730573944 2216 88-89 -1.2981608039273809 2216 90-91 -1.3231973809153104 2216 92-93 -1.606216589876876 2216 94-95 -1.5574199031475011 2216 96-97 -1.579102458829059 2216 98-99 -1.758050545884231 2216 100-101 -1.5929262899722723 2216 102-103 -1.6710828311373511 2216 104-105 -1.6941274689181292 2216 106-107 -1.9156647043739738 2216 108-109 -1.6384383606694115 2216 110-111 -1.5038266819039343 2216 112-113 -1.412811425647206 2216 114-115 -1.4526124246465209 2216 116-117 -1.1123826458363233 2216 118-119 -1.1725685344546655 2216 120-121 -1.1982167589271064 2216 122-123 -0.9592325775288231 2216 124-125 -1.5005065039271663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 36.0 5 118.0 6 338.0 7 834.0 8 1371.0 9 1953.0 10 3031.0 11 5804.0 12 8336.0 13 9050.0 14 11964.0 15 15882.0 16 6864.0 17 2739.0 18 2586.0 19 3238.0 20 4538.0 21 7754.0 22 7497.0 23 2519.0 24 3130.0 25 4095.0 26 5333.0 27 6351.0 28 7928.0 29 10048.0 30 12605.0 31 17184.0 32 23011.0 33 31183.0 34 44112.0 35 67326.0 36 152520.0 37 62306.0 38 5319.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.858592533501756 20.387472230183256 12.24135507578399 15.51258016053099 2 19.740797283363147 21.167796776908588 37.48547131244603 21.605934627282235 3 28.612782578121728 23.091202891501297 28.698041058881184 19.597973471495795 4 15.496895769345814 22.95361527967258 27.61999255860102 33.92949639238059 5 14.88204688966441 34.814619078425835 28.03976418489799 22.263569847011762 6 31.226783407753956 35.80697640948284 9.8503025102613 23.1159376725019 7 27.110557295338655 40.28135298650621 12.164818488702341 20.443271229452797 8 35.43163927646884 27.711921066678325 12.449924305771889 24.406515351080944 9 33.35483012976413 13.677063464737055 17.643404582461166 35.32470182303764 10-11 30.005246675739045 24.804752613773616 18.591540828428236 26.5984598820591 12-13 33.513141283140136 25.895806120757342 19.776870540097903 20.814182056004622 14-15 34.89030257651858 19.301299463126618 24.077505059945093 21.730892900409714 16-17 30.724023034363906 21.071961436933318 25.089585166481758 23.114430362221018 18-19 29.520657874841277 21.076880195438672 25.62718725349277 23.775274676227276 20-21 34.792747199495736 18.05120609780314 25.484907609319407 21.67113909338172 22-23 30.79816876442608 21.12142228634826 25.242704296990997 22.837704652234663 24-25 25.71217246989082 22.38080663995963 27.77249466679055 24.134526223359 26-27 23.18930479724695 21.351784143015635 30.318771986395078 25.140139073342333 28-29 23.95080148437201 19.125772654981883 32.57365840861767 24.34976745202844 30-31 25.46978698132147 20.133662707976953 28.563412816827256 25.833137493874325 32-33 23.80979707388522 19.7425303186809 30.833602043288717 25.614070564145162 34-35 22.951929155303425 21.202672889807054 30.77266409069462 25.072733864194902 36-37 23.23585082680687 20.962382428008507 29.003186262454022 26.798580482730607 38-39 24.34657936781201 17.807545375547214 30.408584873178008 27.43729038346277 40-41 23.28039291771646 17.99172555513655 31.94214446940259 26.785737057744402 42-43 24.2638713544257 18.096567981796948 32.518459007613146 25.121101656164207 44-45 23.34861791994812 18.542717595856583 31.945241465498555 26.163423018696747 46-47 22.308755936668252 18.11159752167442 30.624828071172615 28.954818470484717 48-49 21.75029467006972 19.05290215860628 30.786145132524105 28.410658038799895 50-51 21.745193735323426 19.488667726932388 29.648636684100932 29.11750185364325 52-53 21.642537423554295 19.276523494358912 29.011839633898628 30.06909944818816 54-55 19.96660709503588 19.91623536441624 27.471266252397896 32.64589128814998 56-57 21.123972753721404 20.533903909319953 26.473304804534003 31.868818532424637 58-59 20.710523774910325 20.577352942783907 26.39305617040037 32.3190671119054 60-61 20.998180059353018 22.266035608168053 23.931764067193882 32.80402026528505 62-63 19.560809518344236 23.220548022567993 24.223519317057708 32.99512314203007 64-65 18.950883463680434 22.427534845760487 24.630318863074514 33.99126282748456 66-67 19.59806455961626 23.32384195118041 24.36142673144854 32.71666675775479 68-69 19.54751065275569 23.03172234883471 24.717399106243363 32.70336789216624 70-71 19.112655965634275 22.26148120214458 25.581916457619432 33.04394637460172 72-73 19.344110879747284 20.932232260133098 26.17389815255074 33.54975870756888 74-75 19.196639212707154 20.23722990095078 26.186741577536942 34.37938930880512 76-77 19.30248360869272 20.137670585277608 26.57268194396623 33.98716386206343 78-79 19.702087193192437 20.859270675637028 26.869447040455878 32.56919509071466 80-81 19.46279870071905 21.44660687642439 26.24175880230052 32.84883562055604 82-83 18.204507404553315 20.918386865821734 26.90825057977589 33.96885514984906 84-85 18.682446772656807 19.99921664216396 27.206655262433983 34.111681322745255 86-87 19.37808674868241 20.02772722387092 26.82536039014864 33.768825637298036 88-89 19.15610499909823 20.149147688456768 26.472940452052125 34.22180686039288 90-91 19.224421089450356 19.758379651642592 26.24968346878137 34.767515790125685 92-93 19.103091712984977 19.710285124034694 26.218349155339858 34.968274007640474 94-95 18.773717069367247 19.313140918787653 26.29058203487218 35.62255997697292 96-97 18.688276412366854 19.449135482648625 26.103304859186878 35.759283245797654 98-99 19.358593890901936 19.98837715582809 25.954011429737356 34.69901752353262 100-101 19.603803111205842 20.649676910436696 25.448472361131607 34.298047617225855 102-103 19.148817949460668 21.276818619869236 25.076741741495557 34.49762168917454 104-105 18.975750520568607 22.809558423009587 24.807211993026293 33.40747906339551 106-107 18.52850784906334 24.50980927969336 24.38520073089108 32.57648214035222 108-109 18.103581767073102 25.419141986340428 24.260501093968326 32.21677515261814 110-111 18.186066254700208 26.819963855772876 23.754354067682982 31.23961582184394 112-113 18.38336362021615 27.565799960832 23.52302486257042 30.527811556381423 114-115 17.97066571448351 27.95610970024448 23.67055677209909 30.40266781317292 116-117 17.483414390197023 28.519688209665063 23.931283698415633 30.06561370172228 118-119 17.370017042913346 28.916476972027215 24.274446469675727 29.439059515383715 120-121 17.587176203324002 29.35052111703591 24.284272485639903 28.778030194000188 122-123 17.711239669270924 29.773632581750874 24.05810804532996 28.45701970364824 124-125 16.83417840888901 30.69669759962097 24.317584405053143 28.15153958643688 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 1.0 3 4.5 4 3.5 5 1.0 6 1.5 7 5.5 8 17.0 9 21.5 10 17.5 11 14.0 12 13.0 13 15.5 14 19.0 15 24.5 16 25.5 17 28.5 18 51.5 19 83.5 20 120.0 21 186.0 22 260.5 23 351.0 24 496.5 25 725.5 26 1019.0 27 1297.0 28 1665.0 29 2122.5 30 2626.0 31 3179.0 32 3726.0 33 4149.0 34 4497.0 35 4717.0 36 4698.0 37 4561.5 38 4292.5 39 4037.0 40 3798.0 41 3654.0 42 3680.0 43 3731.5 44 4041.5 45 4772.5 46 5822.5 47 6754.5 48 7846.0 49 10657.5 50 16074.5 51 24824.5 52 37403.0 53 50258.5 54 55937.5 55 53251.0 56 47316.5 57 39415.0 58 29992.5 59 21733.5 60 16046.5 61 11944.5 62 8713.5 63 6679.5 64 5289.0 65 4168.0 66 3541.0 67 3010.0 68 2531.5 69 2075.0 70 1499.0 71 1056.0 72 757.5 73 646.5 74 460.0 75 208.5 76 107.0 77 62.5 78 32.0 79 20.0 80 12.5 81 7.5 82 3.5 83 2.5 84 2.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20257997992417826 2 9.108812046950462E-4 3 0.0 4 0.11568191299627084 5 0.03352042833277769 6 0.0021861148912681104 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0012752336865730646 112-113 2.732643614085138E-4 114-115 9.108812046950462E-4 116-117 0.004463317903005725 118-119 0.0019128505298595967 120-121 3.643524818780184E-4 122-123 0.0019128505298595967 124-125 0.027599700502259898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 548919.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.12427633445993 #Duplication Level Percentage of deduplicated Percentage of total 1 79.92047866090724 40.85876635842529 2 9.429796461232481 9.641830401235236 3 3.405487488873693 5.223092503041742 4 1.8052082216652154 3.6915985586260596 5 1.1507097362083563 2.9414601267334755 6 0.8090734872804647 2.481797792316698 7 0.5748223979330929 2.0571165380617833 8 0.43903516471173326 1.7956284065014225 9 0.35264243486361874 1.6225730358501986 >10 1.9821654453447362 18.05799833661686 >50 0.08752807443025402 2.955920881802668 >100 0.03664793334349585 3.0608787935372788 >500 0.0032022466028297347 1.0061045110271996 >1k 0.002490636246645349 2.0222136772897086 >5k 7.116103561843854E-4 2.583020078934412 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 9168 1.6701917769288366 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 5032 0.9167108444050944 RNA PCR Primer, Index 26 (100% over 22bp) ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 2996 0.5458000178532716 RNA PCR Primer, Index 26 (100% over 24bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1674 0.30496302733190145 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1585 0.2887493418883296 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1454 0.26488425432531937 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 1150 0.20950267707986062 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 1137 0.2071343859476535 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 1121 0.20421956609262934 No Hit TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 904 0.16468732180886433 RNA PCR Primer, Index 26 (95% over 22bp) CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG 703 0.12806989738012348 RNA PCR Primer, Index 26 (96% over 27bp) GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 690 0.12570160624791638 No Hit ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA 576 0.1049335147808693 No Hit TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT 575 0.10475133853993031 RNA PCR Primer, Index 26 (100% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 1.821762409390092E-4 0.0 0.0 0.0 0.0 4 1.821762409390092E-4 0.0 0.0 0.0 0.0 5 0.0010930574456340552 0.0 0.0 0.0 0.0 6 0.0010930574456340552 0.0 0.0 0.0 0.0 7 0.0010930574456340552 0.0 0.0 0.0 0.0 8 0.0012752336865730644 0.0 0.0 0.0 0.0 9 0.0012752336865730644 0.0 0.0 0.0 0.0 10-11 0.003279172336902166 0.0 0.0 2.732643614085138E-4 0.0 12-13 0.005100934746292258 0.0 0.0 3.643524818780184E-4 0.0 14-15 0.005920727830517799 0.0 0.0 3.643524818780184E-4 0.0 16-17 0.006193992191926313 0.0 0.0 6.376168432865322E-4 0.0 18-19 0.007651402119438388 0.0 0.0 8.197930842255414E-4 0.0 20-21 0.008562283324133432 0.0 0.0 0.003279172336902166 0.0 22-23 0.009382076408358975 0.0 0.0 0.018855240937187455 0.0 24-25 0.009928605131176 0.0 0.0 0.043357945343484194 0.0 26-27 0.012023631901974608 0.0 0.0 0.11404232682781978 0.0 28-29 0.014209746793242719 0.0 0.0 0.357247608481397 0.0 30-31 0.016304773564041326 0.0 0.0 0.8731707228206712 0.0 32-33 0.017306742889205876 0.0 0.0 1.5720898711831799 0.0 34-35 0.018217624093900923 0.0 0.0 2.5662256179873535 0.0 36-37 0.022134413274089618 0.0 0.0 3.9453908500161226 0.0 38-39 0.024775968767705256 0.0 0.0 5.838293081492898 0.0 40-41 0.02923928667071098 0.0 0.0 8.07186488352562 0.0 42-43 0.032245194646204636 0.0 0.0 9.770293977800003 0.0 44-45 0.03306498773043017 0.0 0.0 11.26568765154786 0.0 46-47 0.03315607585089968 0.0 0.0 13.048008904774658 0.0 48-49 0.03315607585089968 0.0 0.0 15.003215410652572 0.0 50-51 0.03315607585089968 0.0 0.0 16.95468730359124 0.0 52-53 0.03315607585089968 0.0 0.0 18.7690715752233 0.0 54-55 0.03315607585089968 0.0 0.0 20.461488853546697 0.0 56-57 0.03315607585089968 0.0 0.0 22.16019121218249 0.0 58-59 0.03315607585089968 0.0 0.0 23.846869938916306 0.0 60-61 0.03315607585089968 0.0 0.0 25.680473803967434 0.0 62-63 0.03315607585089968 0.0 0.0 27.433100329921174 0.0 64-65 0.03324716397136918 0.0 0.0 29.131893776677433 0.0 66-67 0.03333825209183869 0.0 0.0 30.93425441640752 0.0 68-69 0.033429340212308194 0.0 0.0 32.827794264727586 0.0 70-71 0.0335204283327777 0.0 0.0 34.885565994254165 0.0 72-73 0.0335204283327777 0.0 0.0 36.90225698144899 0.0 74-75 0.0335204283327777 0.0 0.0 38.69924342207138 0.0 76-77 0.0335204283327777 0.0 0.0 40.59323871099379 0.0 78-79 0.0335204283327777 0.0 0.0 42.47293316500249 0.0 80-81 0.0335204283327777 0.0 0.0 44.30735682313784 0.0 82-83 0.0335204283327777 0.0 0.0 46.265569236991254 0.0 84-85 0.0335204283327777 0.0 0.0 48.35950295034422 0.0 86-87 0.0335204283327777 0.0 0.0 50.03616198382639 0.0 88-89 0.0335204283327777 0.0 0.0 51.57454925043586 0.0 90-91 0.0335204283327777 0.0 0.0 53.51436186395442 0.0 92-93 0.0335204283327777 0.0 0.0 55.15285497495988 0.0 94-95 0.0335204283327777 0.0 0.0 56.625385530469885 0.0 96-97 0.0335204283327777 0.0 0.0 58.12405837655464 0.0 98-99 0.0335204283327777 0.0 0.0 59.421062123919924 0.0 100-101 0.0335204283327777 0.0 0.0 60.69611363425204 0.0 102-103 0.0336115164532472 0.0 0.0 61.92589434871083 0.0 104-105 0.033884780814655714 0.0 0.0 63.08435306484199 0.0 106-107 0.033884780814655714 0.0 0.0 64.12038934706213 0.0 108-109 0.033884780814655714 0.0 0.0 65.12600219704547 0.0 110-111 0.034066957055594725 0.0 0.0 66.1411792996781 0.0 112-113 0.034066957055594725 0.0 0.0 67.14542582785438 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 95 0.0 81.70515 1 TATATTT 25 0.0020450247 71.4988 3 CTATACA 85 1.2732926E-11 63.21982 1 CTTAACA 40 1.740917E-4 59.707607 1 TATTTGG 30 0.0042122453 59.582325 2 TATCGAG 50 7.4501677E-6 59.495495 7 TCTAGAC 15 0.0042377734 59.495487 92-93 TAGTGTT 30 0.0042366465 59.495487 9 GTAGTGT 30 0.0042366465 59.495487 8 GCTTATA 55 1.2991191E-5 54.165756 2 TATTAGG 35 0.007751603 51.070568 2 TATATCG 60 2.1869577E-5 49.602165 5 GGCTTAT 65 3.446124E-5 45.92893 1 TTATAGG 65 3.4891542E-5 45.83256 2 CATCCGA 40 2.294999E-6 44.62162 26-27 GAGATGT 110 9.498763E-9 43.332603 3 GTAAAAT 80 3.6379788E-12 40.903152 14-15 GTTATAG 75 8.016785E-5 39.80507 1 TCTACGG 90 5.1018596E-6 39.721554 2 TGTTAGG 75 8.116642E-5 39.721554 2 >>END_MODULE