FastQCFastQC Report
Thu 26 May 2016
SRR935986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228309
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC203241.6546325069668952No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG182751.4878178048031887No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT174381.4196753422795079No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT154081.254407482156363No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA128591.0468864105041973No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT115600.9411312625731799No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC69020.5619107244186927No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT64800.527554548570433No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG60580.4931983727221733No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC60390.49165153068161194No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG56630.4610403408262904No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG55920.4552600363589292No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG44080.35886735341025755No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA43030.35031901581768105No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC41040.33411788076127424No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA38860.31636990366430595No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA38070.3099382972851294No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA34680.282339378771954No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC32200.26214901950567815No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG29000.23609694303306414No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT25450.20719542069625804No Hit
GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA25400.20678835700137346No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG25180.20499727674388124No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT24170.19677459010721243No Hit
GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA23880.19441362067688178No Hit
GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA23450.19091287290087428No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG22210.1808176932677364No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC22040.17943367670512878No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT21420.1743860868885598No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT21290.17332772128185986No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG19500.15875484100499143No Hit
GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG19220.1564752843136377No Hit
GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG18960.1543585531002378No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC18500.15061356710729953No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA18400.14979943971753035No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG17870.14548456455175368No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGT16690.13587786135247726No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG15520.12635257089217777No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC14850.12089791738072422No Hit
GAGTCGGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC14790.12040944094686272No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT13820.11251240526610161No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC13810.1124309925271247No Hit
GAGTCTGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC13800.11234957978814776No Hit
GTACAGGGGATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA13620.11088415048656323No Hit
GAGTCCGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC13620.11088415048656323No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA13560.11039567405270172No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA13550.1103142613137248No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT13260.10795329188339417No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA13020.10599938614794813No Hit
GAGTAAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC12870.10477819506329433No Hit
TAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT12550.10217298741603292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAG12100.098.459691
GAGTAGT31850.098.3762361
AGTAGTA27250.097.149652
GAGTCCG9750.097.1418461
GAGTCGA4200.096.443611
AGTCGAG1450.094.3642652
GAGTTAG16200.092.293811
AGTCCGT4650.092.114352
AGTACGG4800.091.714542
GAGTCAG74650.091.5266341
GAGTCGT9700.091.501541
GAGTACG7750.091.465881
AGTCGAC1400.089.235782
GAGTAAC10650.088.373361
AGTCGAT1150.087.94252
GAGTAGG11550.087.676011
GAGTCGG9500.086.5305251
AGTAAGG5600.084.986452
AGTCGTA7350.084.986442
GAGTTCG5350.082.393281
Produced by FastQC (version 0.11.5)