##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935986_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1228309 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.229774429724117 33.0 31.0 34.0 30.0 34.0 2 31.238666329075176 33.0 31.0 34.0 28.0 34.0 3 31.460411020353998 34.0 31.0 34.0 28.0 34.0 4 34.60244368477313 37.0 35.0 37.0 32.0 37.0 5 34.75382090337204 37.0 35.0 37.0 32.0 37.0 6 34.87744044861676 37.0 35.0 37.0 32.0 37.0 7 34.852828563496644 37.0 35.0 37.0 32.0 37.0 8 34.80625803441968 37.0 35.0 37.0 32.0 37.0 9 36.42623151014932 39.0 38.0 39.0 32.0 39.0 10-11 36.09814509215515 39.0 37.0 39.0 31.5 39.0 12-13 35.91652792579066 39.0 37.0 39.0 31.0 39.0 14-15 37.420412941694636 40.0 38.0 41.0 31.5 41.0 16-17 37.60923228601273 40.5 38.0 41.0 32.0 41.0 18-19 37.66567288849956 40.5 38.0 41.0 32.0 41.0 20-21 37.799458849524015 41.0 38.0 41.0 32.0 41.0 22-23 37.68570571411591 40.0 38.0 41.0 32.0 41.0 24-25 37.649205126723004 40.0 38.0 41.0 32.0 41.0 26-27 37.625961382681396 40.0 38.0 41.0 31.5 41.0 28-29 37.51359470621806 40.0 37.5 41.0 31.0 41.0 30-31 37.440087551259495 40.0 37.0 41.0 31.0 41.0 32-33 37.363838008188495 40.0 37.0 41.0 30.5 41.0 34-35 37.103728377794184 40.0 37.0 41.0 30.0 41.0 36-37 37.13349165397307 40.0 36.5 41.0 30.5 41.0 38-39 37.269011706337736 40.0 37.0 41.0 31.0 41.0 40-41 37.25956172266099 40.0 36.0 41.0 31.0 41.0 42-43 37.10331927878083 40.0 36.0 41.0 30.0 41.0 44-45 36.91106350275053 40.0 35.0 41.0 30.0 41.0 46-47 36.74855838392457 40.0 35.0 41.0 30.0 41.0 48-49 36.63173965183028 40.0 35.0 41.0 30.0 41.0 50-51 36.41336951858205 39.0 35.0 41.0 30.0 41.0 52-53 36.13940710358713 39.0 35.0 41.0 30.0 41.0 54-55 35.848120057737916 39.0 35.0 41.0 30.0 41.0 56-57 35.56174667774965 38.0 35.0 40.0 30.0 41.0 58-59 35.16894242409687 37.0 35.0 39.5 29.0 41.0 60-61 34.804725846672135 37.0 35.0 39.0 29.0 41.0 62-63 34.322961079011876 36.0 34.0 39.0 28.0 41.0 64-65 33.766395507970714 35.5 34.0 37.5 27.5 40.0 66-67 33.33675606056782 35.0 33.5 37.0 27.5 39.0 68-69 33.1284196403348 35.0 34.0 36.0 28.0 39.0 70-71 33.01365820815446 35.0 34.0 36.0 28.0 38.0 72-73 32.77590533001061 35.0 34.0 35.5 28.0 37.0 74-75 32.47526558870773 35.0 34.0 35.0 27.0 36.5 76-77 32.36537793014624 35.0 34.0 35.0 27.0 36.0 78-79 32.246994038145125 35.0 34.0 35.0 27.0 36.0 80-81 32.09223167785956 35.0 33.0 35.0 27.0 35.5 82-83 31.995513751018677 35.0 33.0 35.0 27.0 35.0 84-85 31.780448567909215 35.0 33.0 35.0 25.5 35.0 86-87 31.641142823182115 35.0 33.0 35.0 25.0 35.0 88-89 31.57933793532409 35.0 33.0 35.0 25.0 35.0 90-91 31.598838728691234 35.0 33.0 35.0 25.0 35.0 92-93 31.571132345362606 35.0 33.0 35.0 25.0 36.0 94-95 31.403877200281038 35.0 33.0 35.0 24.5 35.0 96-97 31.308131341543536 35.0 33.0 35.0 24.0 35.0 98-99 31.158005843806407 35.0 33.0 35.0 24.0 35.0 100-101 31.055873969823555 35.0 33.0 35.0 23.0 35.0 102-103 30.36153606299392 34.0 31.5 35.0 20.0 35.0 104-105 30.858560020320617 35.0 32.0 35.0 21.5 35.0 106-107 30.920617287669472 35.0 33.0 35.0 21.5 35.0 108-109 30.876267698111796 35.0 33.0 35.0 20.5 35.0 110-111 30.804959094169302 35.0 33.0 35.0 20.0 35.0 112-113 30.71695517984481 35.0 32.5 35.0 19.5 35.0 114-115 30.52009388517059 35.0 32.0 35.0 17.5 35.0 116-117 30.31980307886696 35.0 32.0 35.0 12.5 35.0 118-119 30.064747551308344 35.0 31.5 35.0 2.0 35.0 120-121 29.784041312080266 35.0 31.0 35.0 2.0 35.0 122-123 29.24476617854302 35.0 31.0 35.0 2.0 35.0 124-125 26.379242926657703 32.0 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2984822047174447 1101 2 0.39963573000976993 1101 3 0.39978068355311436 1101 4 0.38332218200039136 1101 5 0.31960872791863437 1101 6 0.3096470662516069 1101 7 0.18616357387712412 1101 8 0.1759570728980151 1101 9 0.2523348258399025 1101 10-11 0.457320821684263 1101 12-13 0.293794151301185 1101 14-15 0.44422366542100633 1101 16-17 0.4295736640909098 1101 18-19 0.46995674155912326 1101 20-21 0.2999705848407075 1101 22-23 0.22664491944838971 1101 24-25 0.3114608419548688 1101 26-27 0.33968998172159104 1101 28-29 0.28794750636107835 1101 30-31 0.243753197281805 1101 32-33 0.15757057074305436 1101 34-35 -0.07374984656595984 1101 36-37 0.09919292408002178 1101 38-39 0.18603276893809806 1101 40-41 0.10963775411453724 1101 42-43 -0.01319540160726973 1101 44-45 0.17633997691824277 1101 46-47 0.2480297646298837 1101 48-49 0.2547991681743298 1101 50-51 0.21581637842560042 1101 52-53 0.2457361906549238 1101 54-55 0.22403219621435966 1101 56-57 0.09101141808751834 1101 58-59 0.10563373239856588 1101 60-61 0.15703018236224153 1101 62-63 0.31979583789578214 1101 64-65 0.35204826128730815 1101 66-67 0.15243159768922965 1101 68-69 0.3450642330125717 1101 70-71 0.2859265850915591 1101 72-73 0.32267581177270444 1101 74-75 0.4297065058823648 1101 76-77 0.41061619493187607 1101 78-79 0.41793255117758576 1101 80-81 0.4428781499601584 1101 82-83 0.4095051973871122 1101 84-85 0.27189431597894753 1101 86-87 0.17053805420540158 1101 88-89 0.31592359667768477 1101 90-91 0.2791803967498545 1101 92-93 0.04525281922402513 1101 94-95 0.032518596449257586 1101 96-97 0.10258829061676522 1101 98-99 -0.01715802515948539 1101 100-101 0.057855226485852285 1101 102-103 0.19594928517414445 1101 104-105 0.11303325312891488 1101 106-107 0.19764729806236403 1101 108-109 0.1344932964672978 1101 110-111 0.07305995593087289 1101 112-113 0.10366468763028891 1101 114-115 0.16641672135024166 1101 116-117 0.07578332242403718 1101 118-119 0.06342699816489628 1101 120-121 -0.18895925183204199 1101 122-123 -0.13568452573271017 1101 124-125 -0.04682618563091978 1102 1 0.2663540492143106 1102 2 0.27336469301476285 1102 3 0.33312710140728896 1102 4 0.3727871751273284 1102 5 0.3843896495066872 1102 6 0.3821964301889409 1102 7 0.44063881203631183 1102 8 0.42092291418228456 1102 9 0.4586030737659996 1102 10-11 0.544353790987536 1102 12-13 0.550212330127728 1102 14-15 0.6680894256156833 1102 16-17 0.6487765880386007 1102 18-19 0.4609103448812064 1102 20-21 0.4536148944391627 1102 22-23 0.6410644057432222 1102 24-25 0.5705682956308706 1102 26-27 0.5738946654389565 1102 28-29 0.5557056852318922 1102 30-31 0.4650470006052245 1102 32-33 0.5907878650363116 1102 34-35 0.6332232315405193 1102 36-37 0.6522295044159065 1102 38-39 0.482626452508633 1102 40-41 0.5373333560184719 1102 42-43 0.4486434211990513 1102 44-45 0.4633023340799127 1102 46-47 0.5357699990149669 1102 48-49 0.45442825152247934 1102 50-51 0.4547174705157886 1102 52-53 0.5942679819823979 1102 54-55 0.5502763386162428 1102 56-57 0.41367791554083055 1102 58-59 0.3767600071248083 1102 60-61 0.425797000706055 1102 62-63 0.49852701075829486 1102 64-65 0.4556239878286945 1102 66-67 0.3057468266695693 1102 68-69 0.4934469419658498 1102 70-71 0.5078167548041819 1102 72-73 0.4757054932211915 1102 74-75 0.4141135446659092 1102 76-77 0.3849113831977178 1102 78-79 0.4418285262855193 1102 80-81 0.4260551945948521 1102 82-83 0.34544902790026555 1102 84-85 0.34877153275379413 1102 86-87 0.3194970231648355 1102 88-89 0.4230601821047273 1102 90-91 0.4056058286312698 1102 92-93 0.44846548720455104 1102 94-95 0.35917150715256696 1102 96-97 0.3511441358190126 1102 98-99 0.31466720646973556 1102 100-101 0.4483713591835503 1102 102-103 0.47755153885988477 1102 104-105 0.27420085477313805 1102 106-107 0.06235827157284746 1102 108-109 0.2468699004586199 1102 110-111 0.3003257239467487 1102 112-113 0.26564392308599594 1102 114-115 0.39392671735847884 1102 116-117 0.5009968005626817 1102 118-119 0.29831345428391387 1102 120-121 0.22257653642302344 1102 122-123 0.2122577243080528 1102 124-125 -0.1025686424169514 1103 1 0.050284443802052436 1103 2 0.08803074635198627 1103 3 0.15306348717654927 1103 4 0.1997932111866092 1103 5 0.2893041854145082 1103 6 0.15724944204789182 1103 7 0.17118991098055858 1103 8 0.14036536405943423 1103 9 0.21681850947298642 1103 10-11 0.3266405374618202 1103 12-13 0.21466759821627335 1103 14-15 0.19244332605435943 1103 16-17 0.2650236709839362 1103 18-19 0.20073255881351315 1103 20-21 0.11647805372156483 1103 22-23 0.16444361724090584 1103 24-25 0.21081691844447903 1103 26-27 0.15816178350113574 1103 28-29 0.08857408960298585 1103 30-31 0.22750163199049211 1103 32-33 0.22497413300143876 1103 34-35 0.2795699711709503 1103 36-37 0.252252924999091 1103 38-39 0.15921800034271882 1103 40-41 0.32899394548791605 1103 42-43 0.138239353029725 1103 44-45 0.21786794537115384 1103 46-47 0.2353424681895504 1103 48-49 0.17357724289472998 1103 50-51 0.06375676380319817 1103 52-53 0.1517553460869152 1103 54-55 0.2161831218903032 1103 56-57 0.17694034668150493 1103 58-59 0.08636090355814474 1103 60-61 0.01643991675097567 1103 62-63 -0.10686631479622832 1103 64-65 -0.07974004096038811 1103 66-67 2.025295114549408E-4 1103 68-69 0.10363833160582914 1103 70-71 0.04197539489572932 1103 72-73 -0.017097235346867024 1103 74-75 -0.026883765473094456 1103 76-77 -0.004366407357757396 1103 78-79 0.07042190288849781 1103 80-81 0.09224912543822228 1103 82-83 0.17135976602729386 1103 84-85 0.15645102978625758 1103 86-87 0.1046843457574198 1103 88-89 0.11314100415317085 1103 90-91 -0.06035193297804042 1103 92-93 -0.042484631160551345 1103 94-95 0.04884106656511733 1103 96-97 -0.03732164769065349 1103 98-99 0.1655832738312597 1103 100-101 0.09117941698611176 1103 102-103 0.14864661240331856 1103 104-105 0.09716403537248297 1103 106-107 0.0063389012078758356 1103 108-109 0.012086617986753367 1103 110-111 0.09713743928708851 1103 112-113 -0.0016645189824195938 1103 114-115 0.0022016862245273217 1103 116-117 -0.10783037516742411 1103 118-119 -0.20710273088174347 1103 120-121 -0.07898766402351143 1103 122-123 -0.18608888068908414 1103 124-125 -0.2686268881451497 1104 1 0.028359025522092907 1104 2 0.12447445708308535 1104 3 0.09038665833871917 1104 4 0.03391554280211295 1104 5 0.15411090233088487 1104 6 0.08535171903312744 1104 7 -0.08887270204056108 1104 8 0.02654529096565028 1104 9 0.04643832407762716 1104 10-11 0.09320203452666931 1104 12-13 0.08819085680858763 1104 14-15 0.12094372247678109 1104 16-17 0.18670733267812523 1104 18-19 0.008571710415218092 1104 20-21 0.050098171073464925 1104 22-23 0.007426057899721172 1104 24-25 -0.021771719980257842 1104 26-27 0.18660203775453255 1104 28-29 0.11767533073100367 1104 30-31 0.13336708866368951 1104 32-33 0.04550932363718374 1104 34-35 0.2489801095386639 1104 36-37 0.19811037855521363 1104 38-39 0.16766624413707376 1104 40-41 0.22882668780790283 1104 42-43 0.09537753611171951 1104 44-45 -0.06263180482841335 1104 46-47 0.11535901068006638 1104 48-49 0.15126235994794968 1104 50-51 0.057446417992508714 1104 52-53 0.09697760737816452 1104 54-55 0.28521662501552214 1104 56-57 0.19627764921698088 1104 58-59 0.16021528348287717 1104 60-61 0.10358717546277063 1104 62-63 0.11564375867767751 1104 64-65 0.03540726662539839 1104 66-67 0.15637650329326647 1104 68-69 0.18985223272829188 1104 70-71 0.10763710130420634 1104 72-73 0.07102665480610426 1104 74-75 0.1363704892798978 1104 76-77 0.24099989646473574 1104 78-79 0.2482966103233153 1104 80-81 0.25740098102098585 1104 82-83 0.2811443403076197 1104 84-85 0.2192159336402426 1104 86-87 0.1832925704366204 1104 88-89 0.13189996438602947 1104 90-91 0.1346867114201835 1104 92-93 0.18339129948181565 1104 94-95 0.17283881021557335 1104 96-97 0.11266501378089089 1104 98-99 0.19475154700432284 1104 100-101 0.3103677556503328 1104 102-103 0.38770630384548355 1104 104-105 0.36004216398712074 1104 106-107 0.2469730820525271 1104 108-109 0.3351313526280961 1104 110-111 0.3390834117981818 1104 112-113 0.34736760616759454 1104 114-115 0.23130856143835032 1104 116-117 0.1409158710380538 1104 118-119 0.20444755231591927 1104 120-121 0.23377723983047716 1104 122-123 0.21483690821869317 1104 124-125 0.39249812339454593 1105 1 0.48051687970083634 1105 2 0.406896846033451 1105 3 0.44495135866924684 1105 4 0.3989945084543578 1105 5 0.40747289855433877 1105 6 0.38818465224942145 1105 7 0.4883082735216888 1105 8 0.45017871098919926 1105 9 0.4149935039538164 1105 10-11 0.6325181906056443 1105 12-13 0.4963206947334129 1105 14-15 0.5985891845743794 1105 16-17 0.6490294450869385 1105 18-19 0.6142882314689118 1105 20-21 0.6217695578793467 1105 22-23 0.5340538849994871 1105 24-25 0.5894687692061282 1105 26-27 0.6101312072997942 1105 28-29 0.4536530531554348 1105 30-31 0.49172696392913906 1105 32-33 0.5520463841920034 1105 34-35 0.5349705446389876 1105 36-37 0.4650491204512974 1105 38-39 0.47152910018187555 1105 40-41 0.48496831551408803 1105 42-43 0.46963528251050235 1105 44-45 0.4705335730321849 1105 46-47 0.40373135678643024 1105 48-49 0.3403590017753402 1105 50-51 0.2821815270045249 1105 52-53 0.3168294475134914 1105 54-55 0.31699798790588574 1105 56-57 0.43681079073815 1105 58-59 0.26367146462118995 1105 60-61 0.22014623646334996 1105 62-63 0.30074080966367944 1105 64-65 0.2987202814642558 1105 66-67 0.3186425361344618 1105 68-69 0.3599620519468587 1105 70-71 0.34214103569764376 1105 72-73 0.2754687055927576 1105 74-75 0.2656982730454587 1105 76-77 0.3005752865543343 1105 78-79 0.2508103867462381 1105 80-81 0.25300644753697554 1105 82-83 0.3228714929492753 1105 84-85 0.38093636719150936 1105 86-87 0.39719739465201087 1105 88-89 0.4912346960047742 1105 90-91 0.467404563873405 1105 92-93 0.4515375821842724 1105 94-95 0.5127148142391817 1105 96-97 0.5228198927359529 1105 98-99 0.6311567944309076 1105 100-101 0.6094412989704914 1105 102-103 0.5244955133000531 1105 104-105 0.5855576809681828 1105 106-107 0.49085171619478984 1105 108-109 0.5047037426569148 1105 110-111 0.5335000960577965 1105 112-113 0.47331754371534984 1105 114-115 0.5648084558806623 1105 116-117 0.5177846180518806 1105 118-119 0.49661245892514216 1105 120-121 0.6134391806933301 1105 122-123 0.7014912129791142 1105 124-125 0.7370939942912749 1106 1 -0.1125142766395193 1106 2 0.11336579412634862 1106 3 0.16842027908104384 1106 4 -0.5704115808851498 1106 5 0.17820919074600283 1106 6 0.11686076572181747 1106 7 0.31056664894288133 1106 8 0.2583612452193975 1106 9 0.3138203284531045 1106 10-11 0.3465683009525975 1106 12-13 0.29572328618800015 1106 14-15 0.3285754320988161 1106 16-17 0.44682433062557436 1106 18-19 0.24977119123317237 1106 20-21 0.43118578856862655 1106 22-23 0.3403528459515144 1106 24-25 0.25865661820822083 1106 26-27 0.28467629352643087 1106 28-29 0.20967935523680126 1106 30-31 0.22641899906903973 1106 32-33 0.44087152266043006 1106 34-35 0.40316980077223263 1106 36-37 0.33206721131074346 1106 38-39 0.2835652844172287 1106 40-41 0.41947513890612953 1106 42-43 0.328759225910197 1106 44-45 0.2051755934507753 1106 46-47 0.20897183008296594 1106 48-49 0.14659505112823012 1106 50-51 0.06708960057586921 1106 52-53 0.1312663383719297 1106 54-55 0.12581561472937608 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-0.9644336625783048 2216 74-75 -0.9002632348524777 2216 76-77 -0.9879400893528967 2216 78-79 -1.238797908101681 2216 80-81 -1.1983309110133682 2216 82-83 -1.345678040210931 2216 84-85 -1.3021813037992054 2216 86-87 -1.2893735483834021 2216 88-89 -1.1966196514199083 2216 90-91 -1.3229279717201408 2216 92-93 -1.2009245843247456 2216 94-95 -1.0630801095434919 2216 96-97 -1.1905581417998512 2216 98-99 -1.1134476608763784 2216 100-101 -1.0474942024045966 2216 102-103 -1.1124507171865687 2216 104-105 -1.4579699284921581 2216 106-107 -1.240603791167203 2216 108-109 -1.4740692296515903 2216 110-111 -1.6547914395167176 2216 112-113 -1.8296106396444465 2216 114-115 -1.730682385541943 2216 116-117 -1.7317780652135326 2216 118-119 -1.8065251889923246 2216 120-121 -1.5112139619436746 2216 122-123 -1.3219518442930465 2216 124-125 -1.3397456805847554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16056.0 3 3574.0 4 2360.0 5 1707.0 6 2067.0 7 2245.0 8 2541.0 9 2970.0 10 3737.0 11 4343.0 12 4134.0 13 3677.0 14 3587.0 15 3412.0 16 3393.0 17 3320.0 18 3213.0 19 3154.0 20 3226.0 21 3479.0 22 4210.0 23 5700.0 24 8621.0 25 12572.0 26 15061.0 27 13004.0 28 14627.0 29 18994.0 30 21878.0 31 27568.0 32 37141.0 33 50486.0 34 85248.0 35 121899.0 36 270337.0 37 411879.0 38 32822.0 39 67.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 77.81916507115389 7.765771113771206 4.95370023060188 9.461363584473025 2 9.401855552244202 36.17724318417586 44.65311400230274 9.767787261277194 3 43.523470042435946 19.512800805077983 15.639747142982763 21.323982009503307 4 15.366908419664965 14.802709522657931 51.31376347290518 18.516618584771923 5 16.93962555403218 32.05595195836739 26.961461179482004 24.042961308118425 6 30.33471219375581 39.89834805411342 13.52794777209969 16.238991980031084 7 49.57119666010476 20.149686314929063 15.278164490491752 15.000952534474422 8 46.97506405157668 20.57376535344991 14.804035147564901 17.64713544740851 9 64.90158421048775 8.674934401685569 7.833859395315022 18.589621992511656 10-11 43.16605186479949 25.66740128094803 17.608598487839785 13.557948366412687 12-13 28.899324192853754 31.09445587388841 24.338745380844724 15.667474552413113 14-15 24.9359892339794 31.086680957316116 22.185500554013686 21.791829254690796 16-17 21.89774722809977 37.74160247950638 20.46622633229912 19.89442396009473 18-19 22.99665637881022 27.038147567102417 24.60402879080101 25.361167263286355 20-21 23.284735355680045 28.945200271267247 21.245794014372603 26.524270358680102 22-23 24.40778338349715 24.91445556452 22.081780724557095 28.595980327425753 24-25 20.89209636988738 22.151551441860313 36.64289686064337 20.31345532760893 26-27 15.532615916684577 22.993114037658675 32.88649478627302 28.58777525938373 28-29 20.385579268590558 23.959619086080163 21.230186516246487 34.424615129082795 30-31 20.389169174857464 30.311998039581244 19.64986009220807 29.64897269335322 32-33 31.81206846160046 25.65885294335546 17.10807296860969 25.42100562643439 34-35 30.892226630269743 16.550802770312682 25.802098657585347 26.754871941832224 36-37 25.36682544864525 25.20432562164732 30.20884809929749 19.220000830409937 38-39 22.58234695015668 31.37850492017888 23.718909492643952 22.32023863702049 40-41 19.59160927747008 30.78752984794543 26.07141199812099 23.549448876463497 42-43 21.162671607877172 21.829238408250692 23.56911005292642 33.43897993094572 44-45 22.465804614311217 22.748876707733967 22.85577163401066 31.929547043944158 46-47 24.36894950700516 21.593182171587117 22.780912620521384 31.25695570088634 48-49 15.71803186331778 21.502773324953246 25.20851837770463 37.570676434024335 50-51 21.975505339253125 24.68883020837192 23.878121823629815 29.45754262874514 52-53 24.964117335295924 21.37426331647818 28.91817124192691 24.743448106298985 54-55 19.23343393234113 23.9092117700025 30.460209930888727 26.397144366767645 56-57 19.81550162480201 26.92677339110407 26.145085477652714 27.112639506441198 58-59 24.333412846441735 22.34087676635114 31.880373749602096 21.445336637605035 60-61 25.354410005951273 29.934772113531693 27.99918424435545 16.711633636161586 62-63 27.1711759825907 25.074268771131692 25.684335130655235 22.070220115622373 64-65 33.533246382758385 17.111392804921337 29.057001475228855 20.29835933709142 66-67 33.57716468507898 23.23146963139538 23.010555984329663 20.18080969919597 68-69 23.170034191576228 29.04118255061261 24.981182216797958 22.8076010410132 70-71 25.304139720240315 28.849179270618393 21.41432705727924 24.43235395186205 72-73 23.739780144629908 28.53894484890795 22.31757927066309 25.403695735799058 74-75 19.891922215586437 28.76946106443672 27.797339091402428 23.541277628574417 76-77 22.418432244786306 29.056406231682057 23.096073936093998 25.42908758743764 78-79 23.2721496804721 31.57906091892308 17.598153214635065 27.550636185969758 80-81 21.637836110551394 21.566273642989586 24.893236369853394 31.902653876605626 82-83 24.59869625639802 16.43487103000955 29.46074644083858 29.505686272753845 84-85 26.690886852941965 17.862787745340334 33.85344439386697 21.59288100785073 86-87 30.012440220993035 21.95617590211956 24.669007078225217 23.362376798662186 88-89 24.124224179067046 26.635392254737983 26.578961511464477 22.661422054730494 90-91 19.114301950325164 29.148796555912686 25.698847520328776 26.03805397343337 92-93 24.38735129589369 21.81384403677195 27.05519049596535 26.74361417136901 94-95 22.031650130484522 20.370730754041762 30.362710332501436 27.234908782972276 96-97 22.14006682798308 19.92223756501236 29.328430877654636 28.609264729349924 98-99 20.807712958993967 21.71756954188837 32.35537285964289 25.11934463947477 100-101 18.792192461125133 27.333428315558088 29.0812912155011 24.79308800781568 102-103 20.066693315769893 26.704233218188584 26.635073096427693 26.594000369613834 104-105 20.32092244340362 22.224524404808914 25.75207420318928 31.70247894859819 106-107 19.616191237602724 24.11267172191674 23.113236733225108 33.15790030725543 108-109 22.329804633850276 25.186374112702907 25.236809304499115 27.247011948947698 110-111 23.691391986869753 27.221000578844574 22.561342463500633 26.52626497078504 112-113 20.524955324250282 28.10204389010783 24.340610358260843 27.032390427381042 114-115 23.84108494469881 22.207431271949805 26.863527429952978 27.08795635339841 116-117 26.25927189331023 20.797413354457227 26.5256950816122 26.41761967062034 118-119 21.752132939478415 22.5960204683973 28.58474989520151 27.067096696922775 120-121 20.49795405955942 23.942998617585808 28.677889351502827 26.88115797135195 122-123 21.705080173956866 25.966559344685937 29.704604695109367 22.62375578624783 124-125 21.16282884937305 28.989021554513926 24.461382005992398 25.386767590120623 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19.0 1 27.0 2 38.5 3 42.5 4 48.5 5 45.5 6 44.0 7 55.5 8 59.5 9 67.5 10 90.0 11 98.5 12 103.5 13 146.5 14 185.0 15 216.0 16 276.5 17 355.0 18 433.5 19 491.5 20 577.5 21 684.0 22 807.0 23 995.5 24 1188.5 25 1413.5 26 1732.0 27 2040.5 28 2280.5 29 2603.5 30 3100.0 31 3621.0 32 4159.5 33 4803.5 34 5546.0 35 6040.5 36 6667.5 37 7538.5 38 7843.5 39 7908.5 40 7943.5 41 8204.0 42 8607.5 43 9163.5 44 10359.0 45 15276.0 46 34101.5 47 77857.0 48 121057.0 49 149438.5 50 153659.5 51 127293.0 52 93031.5 53 63376.5 54 45660.5 55 37600.0 56 32778.0 57 27957.0 58 23477.5 59 18591.0 60 15563.0 61 13347.0 62 10102.0 63 7247.0 64 5456.0 65 4705.5 66 4254.5 67 3865.5 68 3877.0 69 3789.5 70 3277.0 71 2550.0 72 1887.0 73 1462.0 74 1189.0 75 1091.5 76 931.0 77 793.0 78 758.0 79 616.5 80 540.0 81 478.5 82 311.0 83 160.0 84 72.0 85 46.5 86 38.5 87 26.5 88 13.5 89 10.0 90 9.0 91 8.5 92 5.5 93 2.0 94 1.0 95 2.0 96 2.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15899907922192216 2 0.01717808792412984 3 0.00854833759257646 4 0.01595689683947606 5 0.0033379222980536656 6 0.0 7 5.698891728384307E-4 8 1.6282547795383733E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0010583656066999428 28-29 0.00854833759257646 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.070636948845933E-5 52-53 0.0 54-55 0.0 56-57 4.4777006437305274E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.002035318474422967 66-67 8.141273897691866E-5 68-69 0.006716550965595791 70-71 0.020760248439114264 72-73 1.2211910846537802E-4 74-75 0.009728822307741782 76-77 4.070636948845934E-4 78-79 0.0010176592372114835 80-81 9.362464982345648E-4 82-83 0.0 84-85 0.02198143952376804 86-87 0.0028494458641921535 88-89 0.020760248439114264 90-91 8.141273897691866E-5 92-93 0.016201135056406815 94-95 0.01579407136152222 96-97 0.006594431857130412 98-99 0.0031343904506113693 100-101 7.32714650792268E-4 102-103 0.0 104-105 4.884764338615121E-4 106-107 0.00122119108465378 108-109 0.0 110-111 0.0 112-113 0.0011397783456768614 114-115 0.0065537254876419536 116-117 0.0 118-119 0.07762704661449196 120-121 0.0018724929964691296 122-123 0.005983836314803523 124-125 0.2515653634386787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1228309.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.859924775823522 #Duplication Level Percentage of deduplicated Percentage of total 1 79.57599692880297 24.55709279184023 2 10.051972905100632 6.204062554000434 3 3.1837224611637365 2.947483069758379 4 1.5852855408868272 1.9568717015987274 5 0.9883703945007932 1.525051801247275 6 0.6905917195227608 1.2786965109167407 7 0.47672042199174364 1.0298089453234838 8 0.3808475174016194 0.9402340590458491 9 0.2911144754756843 0.8085393732899644 >10 2.2337624830402096 13.774651333482089 >50 0.28200919595120744 6.14676377655162 >100 0.21216527381826988 13.331003343384296 >500 0.03004576548434753 6.424589723773173 >1k 0.014232204703111987 8.290396639374597 >5k 0.001581356078123554 2.9877310914430146 >10k+ 0.001581356078123554 7.797023284970141 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 20324 1.6546325069668952 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 18275 1.4878178048031887 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 17438 1.4196753422795079 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 15408 1.254407482156363 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 12859 1.0468864105041973 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 11560 0.9411312625731799 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 6902 0.5619107244186927 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 6480 0.527554548570433 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 6058 0.4931983727221733 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 6039 0.49165153068161194 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 5663 0.4610403408262904 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 5592 0.4552600363589292 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 4408 0.35886735341025755 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 4303 0.35031901581768105 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 4104 0.33411788076127424 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 3886 0.31636990366430595 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 3807 0.3099382972851294 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 3468 0.282339378771954 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 3220 0.26214901950567815 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2900 0.23609694303306414 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 2545 0.20719542069625804 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2540 0.20678835700137346 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 2518 0.20499727674388124 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 2417 0.19677459010721243 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2388 0.19441362067688178 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2345 0.19091287290087428 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 2221 0.1808176932677364 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 2204 0.17943367670512878 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2142 0.1743860868885598 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 2129 0.17332772128185986 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1950 0.15875484100499143 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1922 0.1564752843136377 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1896 0.1543585531002378 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1850 0.15061356710729953 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 1840 0.14979943971753035 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1787 0.14548456455175368 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGT 1669 0.13587786135247726 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1552 0.12635257089217777 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 1485 0.12089791738072422 No Hit GAGTCGGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1479 0.12040944094686272 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1382 0.11251240526610161 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 1381 0.1124309925271247 No Hit GAGTCTGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1380 0.11234957978814776 No Hit GTACAGGGGATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1362 0.11088415048656323 No Hit GAGTCCGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1362 0.11088415048656323 No Hit TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA 1356 0.11039567405270172 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1355 0.1103142613137248 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1326 0.10795329188339417 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1302 0.10599938614794813 No Hit GAGTAAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1287 0.10477819506329433 No Hit TAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT 1255 0.10217298741603292 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.920082813038087E-4 0.0 22-23 0.0 0.0 0.0 0.0017096675185152921 0.0 24-25 0.0 0.0 0.0 0.0029715649726575317 0.0 26-27 0.0 0.0 0.0 0.005088296186057417 0.0 28-29 0.0 0.0 0.0 0.012293323585514718 0.0 30-31 0.0 0.0 0.0 0.03981082935971323 0.0 32-33 0.0 0.0 0.0 0.09846870779258314 0.0 34-35 4.070636948845934E-5 0.0 0.0 0.1627847715843489 0.0 36-37 8.141273897691868E-5 0.0 0.0 0.23984192902600243 0.0 38-39 8.141273897691868E-5 0.0 0.0 0.368392643870557 0.0 40-41 8.141273897691868E-5 0.0 0.0 0.5898352938877758 0.0 42-43 1.6282547795383736E-4 0.0 0.0 1.1814209616635554 0.0 44-45 1.6282547795383736E-4 0.0 0.0 1.9973394316902344 0.0 46-47 1.6282547795383736E-4 0.0 0.0 2.7904216284338874 0.0 48-49 1.6282547795383736E-4 0.0 0.0 3.040684388048936 0.0 50-51 1.6282547795383736E-4 0.0 0.0 3.39434132616467 0.0 52-53 1.6282547795383736E-4 0.0 0.0 3.666829763520417 0.0 54-55 1.6282547795383736E-4 0.0 0.0 3.9218551683656147 0.0 56-57 1.6282547795383736E-4 0.0 0.0 4.411105023247408 0.0 58-59 1.6282547795383736E-4 0.0 0.0 4.989583240047903 0.0 60-61 1.6282547795383736E-4 0.0 0.0 5.900266138243715 0.0 62-63 1.6282547795383736E-4 0.0 0.0 6.980206120772541 0.0 64-65 1.6282547795383736E-4 0.0 0.0 7.843873162209183 0.0 66-67 1.6282547795383736E-4 0.0 0.0 8.490534547902849 0.0 68-69 1.6282547795383736E-4 0.0 0.0 9.000544651223755 0.0 70-71 1.6282547795383736E-4 0.0 0.0 9.621683143248157 0.0 72-73 1.6282547795383736E-4 0.0 0.0 10.221125140335207 0.0 74-75 1.6282547795383736E-4 0.0 0.0 10.87132798017437 0.0 76-77 1.6282547795383736E-4 0.0 0.0 11.666404789022957 0.0 78-79 1.6282547795383736E-4 0.0 0.0 12.76470334419108 0.0 80-81 1.6282547795383736E-4 0.0 0.0 15.881060873119061 0.0 82-83 1.6282547795383736E-4 0.0 0.0 17.885320387622333 0.0 84-85 1.6282547795383736E-4 0.0 0.0 18.572077547262133 0.0 86-87 1.6282547795383736E-4 0.0 0.0 19.139320806083813 0.0 88-89 1.6282547795383736E-4 0.0 0.0 19.906147394507407 0.0 90-91 1.6282547795383736E-4 0.0 0.0 21.053130767583724 0.0 92-93 1.6282547795383736E-4 0.0 0.0 22.62272766868923 0.0 94-95 1.6282547795383736E-4 0.0 0.0 24.589048846829257 0.0 96-97 1.6282547795383736E-4 0.0 0.0 26.608451130782235 0.0 98-99 1.6282547795383736E-4 0.0 0.0 28.51603301775042 0.0 100-101 1.6282547795383736E-4 0.0 0.0 30.1700142228055 0.0 102-103 1.6282547795383736E-4 0.0 0.0 31.580123568255217 0.0 104-105 1.6282547795383736E-4 0.0 0.0 33.04205212206375 0.0 106-107 1.6282547795383736E-4 0.0 0.0 34.44597409935122 0.0 108-109 1.6282547795383736E-4 0.0 0.0 35.46977999835546 0.0 110-111 1.6282547795383736E-4 0.0 0.0 36.58240719558352 0.0 112-113 1.6282547795383736E-4 0.0 0.0 41.17929608917626 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAAG 1210 0.0 98.45969 1 GAGTAGT 3185 0.0 98.376236 1 AGTAGTA 2725 0.0 97.14965 2 GAGTCCG 975 0.0 97.141846 1 GAGTCGA 420 0.0 96.44361 1 AGTCGAG 145 0.0 94.364265 2 GAGTTAG 1620 0.0 92.29381 1 AGTCCGT 465 0.0 92.11435 2 AGTACGG 480 0.0 91.71454 2 GAGTCAG 7465 0.0 91.526634 1 GAGTCGT 970 0.0 91.50154 1 GAGTACG 775 0.0 91.46588 1 AGTCGAC 140 0.0 89.23578 2 GAGTAAC 1065 0.0 88.37336 1 AGTCGAT 115 0.0 87.9425 2 GAGTAGG 1155 0.0 87.67601 1 GAGTCGG 950 0.0 86.530525 1 AGTAAGG 560 0.0 84.98645 2 AGTCGTA 735 0.0 84.98644 2 GAGTTCG 535 0.0 82.39328 1 >>END_MODULE