FastQCFastQC Report
Thu 26 May 2016
SRR935985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102174
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC135111.2258500019053253No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT119651.0855817683959157No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG108100.9807888772553154No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT106760.9686310872874881No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT81420.7387218352093227No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA78250.7099604962555821No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT48630.441218900101073No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC44840.40683231504281536No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC39460.3580196956197479No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG36790.33379484545997273No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG35610.32308873190621445No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG32440.29432739295247395No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG31550.28625244289921553No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC26700.24224850159775135No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA26430.23979879764901005No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA26000.23589741728619984No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA25190.2285483054399759No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA23030.20895067385004545No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC22850.2073175378842179No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC19450.17646941408525332No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT19390.17592503542997748No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG17160.15569229540889187No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG17090.15505718697773674No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC17020.15442207854658158No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT16740.15188164482196095No Hit
GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA16690.15142799594256443No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT16500.1497041302008576No Hit
GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA16270.1476173453556335No Hit
GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA15930.14453253297573704No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT15210.1379999891124269No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG14910.13527809583604766No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT14340.13010649861092713No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG12660.11486389626320344No Hit
GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG12600.1143195176079276No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA12060.10942010971044498No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT11910.10805916307225537No Hit
GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG11750.10660748665818646No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA11600.10524654001999685No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG11520.10452070181296239No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC11220.10179880853658314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAG13550.095.8475341
AGTTAGG3900.090.015812
AGTAGTA19250.089.948452
GAGTAGT23250.089.938971
GAGTCGT8200.089.362441
GAGTCCG7250.087.10271
AGTTAGT9300.087.013642
GAGTACG6300.086.998361
GAGTCAG56850.084.987321
GAGTAAG10600.083.180141
GTTAGTG10100.080.121473
AGTCCGT3050.079.986262
AGTCGTA5000.078.54262
GAGTTAA3650.078.345151
GAGTCGA4200.078.014831
AGTAAGG5900.077.655112
GAGTAGG11250.076.255951
GAGTAGA6050.075.8226851
GTGGGGT71600.073.9451757
GAGTAAC9050.073.728131
Produced by FastQC (version 0.11.5)