FastQCFastQC Report
Thu 26 May 2016
SRR935984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463978
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA16080.3465681562487877No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT15970.34419735418489666No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT13940.30044527973309076No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA11100.23923548099263328No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC10680.23018332765777685No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC10070.21703615257619974No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA6040.1301785860536491No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT5890.12694567414834323No Hit
GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT5480.11810904827384058No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA4800.10345318096978737No Hit
GTGGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCC4640.10000474160412778No Hit
GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT4640.10000474160412778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG603.1089257E-759.6104741
ATCAAGG350.00779934150.990078
TAACAAC2750.047.688381
AGTCGTT505.334415E-447.5907337
GTGGAAT2150.047.1338651
TCCTAGG653.5164925E-545.7701872
TGTATGG1109.607902E-943.273632
ACCACGT558.531323E-443.26436
GATTGTG558.531323E-443.26439
TGGAATC2500.042.8408932
GTCCACG1053.2110438E-739.740321
AGTCGTG801.19774064E-437.180267
GTGTAAG1304.125468E-836.683371
TATATGG1304.1927706E-836.616152
GCAGAGA6400.036.258573
ACAAAGT1156.656246E-736.2103429
GAGTATA2500.035.7662851
CCTAGGG1001.0606724E-535.7007453
TAGGGCA851.7097879E-434.9969565
AGTATAT1209.2710616E-734.7090572
Produced by FastQC (version 0.11.5)