##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935984_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 463978 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.463554306454185 31.0 30.0 34.0 12.0 34.0 2 28.420093194073857 31.0 30.0 34.0 10.0 34.0 3 28.403116096021794 31.0 30.0 34.0 10.0 34.0 4 31.443141700684084 37.0 33.0 37.0 10.0 37.0 5 31.691754781476707 37.0 33.0 37.0 10.0 37.0 6 31.853189159830855 37.0 35.0 37.0 10.0 37.0 7 31.840078624417536 37.0 35.0 37.0 8.0 37.0 8 31.554493963075835 37.0 35.0 37.0 8.0 37.0 9 32.693425119294446 39.0 35.0 39.0 7.0 39.0 10-11 32.77922121307476 39.0 34.0 39.0 7.0 39.0 12-13 32.78104780830125 39.0 34.0 39.0 7.0 39.0 14-15 33.80653177521348 40.0 34.0 41.0 8.0 41.0 16-17 33.94673777636008 39.5 34.0 41.0 9.0 41.0 18-19 34.181566798425784 40.0 34.0 41.0 11.5 41.0 20-21 34.37270301609128 40.0 34.0 41.0 13.5 41.0 22-23 34.14750376095418 40.0 33.5 41.0 9.0 41.0 24-25 33.887155209945306 40.0 33.0 41.0 5.0 41.0 26-27 33.70801309544849 39.0 33.0 41.0 2.0 41.0 28-29 33.45672424123558 39.0 33.0 41.0 2.0 41.0 30-31 33.18025639146684 39.0 33.0 41.0 2.0 41.0 32-33 32.827540314411465 39.0 32.0 41.0 2.0 41.0 34-35 32.456602468220474 39.0 32.0 41.0 2.0 41.0 36-37 32.234522973072 39.0 32.0 41.0 2.0 41.0 38-39 32.04018293970835 39.0 32.0 41.0 2.0 41.0 40-41 31.78054886223054 38.5 32.0 41.0 2.0 41.0 42-43 31.491944661169278 38.0 31.5 41.0 2.0 41.0 44-45 31.137576566130292 37.0 31.0 40.5 2.0 41.0 46-47 30.773210152205493 37.0 31.0 40.0 2.0 41.0 48-49 30.386146541430843 36.0 31.0 40.0 2.0 41.0 50-51 29.997704632547233 35.5 30.0 39.0 2.0 41.0 52-53 29.66488712826901 35.0 30.0 39.0 2.0 41.0 54-55 29.307411127251726 35.0 30.0 37.5 2.0 41.0 56-57 28.974692765605266 35.0 29.0 37.0 2.0 40.0 58-59 28.653365030238504 35.0 29.0 36.5 2.0 39.5 60-61 28.378036243097732 35.0 29.0 36.0 2.0 39.0 62-63 28.10021811378988 35.0 28.0 35.5 2.0 39.0 64-65 27.910149834690436 35.0 28.0 35.0 2.0 37.0 66-67 27.79799365487157 35.0 28.5 35.0 2.0 37.0 68-69 27.705900926337023 35.0 29.0 35.0 2.0 36.0 70-71 27.543190625417587 35.0 28.5 35.0 2.0 36.0 72-73 27.391521796292068 35.0 27.5 35.0 2.0 35.5 74-75 27.23350568345913 35.0 27.0 35.0 2.0 35.0 76-77 27.073119846199603 35.0 27.0 35.0 2.0 35.0 78-79 26.91937656526818 35.0 25.5 35.0 2.0 35.0 80-81 26.740338981589645 35.0 25.0 35.0 2.0 35.0 82-83 26.58961092982857 34.0 25.0 35.0 2.0 35.0 84-85 26.46218786235554 34.0 24.0 35.0 2.0 35.0 86-87 26.335319778092927 34.0 24.0 35.0 2.0 35.0 88-89 26.110609770290832 34.0 21.5 35.0 2.0 35.0 90-91 25.848947579411096 34.0 19.5 35.0 2.0 35.0 92-93 25.5782343128338 34.0 18.0 35.0 2.0 35.0 94-95 25.349339839388936 34.0 15.0 35.0 2.0 35.0 96-97 25.139401006082185 33.5 11.5 35.0 2.0 35.0 98-99 24.868116591734953 33.0 7.0 35.0 2.0 35.0 100-101 24.640339628172025 33.0 3.5 35.0 2.0 35.0 102-103 23.987835630137635 31.5 2.0 34.5 2.0 35.0 104-105 24.413157520399672 33.0 2.0 35.0 2.0 35.0 106-107 24.411524899887496 33.0 2.0 35.0 2.0 35.0 108-109 24.203843716727945 33.0 2.0 35.0 2.0 35.0 110-111 23.861291914702853 33.0 2.0 35.0 2.0 35.0 112-113 23.41874183689744 33.0 2.0 35.0 2.0 35.0 114-115 22.873763411196222 32.0 2.0 35.0 2.0 35.0 116-117 22.272203638965642 32.0 2.0 35.0 2.0 35.0 118-119 21.488889559418766 31.0 2.0 35.0 2.0 35.0 120-121 20.691197642991696 30.5 2.0 35.0 2.0 35.0 122-123 19.873893805309734 29.5 2.0 35.0 2.0 35.0 124-125 17.440497178745545 22.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6793186990731428 1101 2 0.9186340498346013 1101 3 0.5629386993915055 1101 4 0.8052959767772592 1101 5 0.408337370111969 1101 6 0.3612177598035835 1101 7 0.8469176694359817 1101 8 0.7709653391609095 1101 9 0.5010494875347575 1101 10-11 0.7347453123516701 1101 12-13 0.5168564248182719 1101 14-15 0.7370205577867353 1101 16-17 0.5695204190097627 1101 18-19 0.44550229082619097 1101 20-21 0.4221172855086408 1101 22-23 0.34176978671041525 1101 24-25 0.5563874860261606 1101 26-27 0.5160633969836539 1101 28-29 0.6094306844207722 1101 30-31 0.6075438354404099 1101 32-33 0.4803632748810287 1101 34-35 0.4563456462452251 1101 36-37 0.5173491330995859 1101 38-39 0.5992907648596244 1101 40-41 0.5775151500671676 1101 42-43 0.7335344297801285 1101 44-45 0.8852790232495629 1101 46-47 0.7694470024579694 1101 48-49 0.6961823624402221 1101 50-51 0.9982650248382008 1101 52-53 0.9973572420274159 1101 54-55 0.7550926106283242 1101 56-57 0.7039521242381497 1101 58-59 0.5229441004937385 1101 60-61 0.7704872861035597 1101 62-63 0.629137027168742 1101 64-65 0.5358360237630464 1101 66-67 0.38424160909636385 1101 68-69 0.5713132322283734 1101 70-71 0.3476474435825665 1101 72-73 0.48891852981212836 1101 74-75 0.6568540796303921 1101 76-77 0.8022885296397 1101 78-79 0.8455799101228862 1101 80-81 0.5986141203371034 1101 82-83 0.7071491739458367 1101 84-85 0.5983933954631517 1101 86-87 0.6551338884465601 1101 88-89 0.7704884333244735 1101 90-91 0.5712290355923315 1101 92-93 0.3343439915791606 1101 94-95 0.28962242212547196 1101 96-97 0.33187451659212286 1101 98-99 0.03788372820285346 1101 100-101 0.10287816460913746 1101 102-103 0.13290888743989626 1101 104-105 -0.22029984253876833 1101 106-107 0.04637280868501037 1101 108-109 0.11900105572189545 1101 110-111 0.09959403093688124 1101 112-113 0.13196796059562033 1101 114-115 0.035832715829517525 1101 116-117 -0.04707479379711188 1101 118-119 0.1825783576618747 1101 120-121 -0.4728600239294174 1101 122-123 -0.3530619859211761 1101 124-125 -0.18269953565091868 1102 1 0.5803588460445575 1102 2 0.5383968422915082 1102 3 0.46355468253302234 1102 4 0.3892696083761109 1102 5 0.3581374457590236 1102 6 0.009956075035660206 1102 7 0.3075379752662073 1102 8 0.20636005467395435 1102 9 0.10227064709600597 1102 10-11 0.5128698057865861 1102 12-13 0.4252978253694124 1102 14-15 0.39802044971951744 1102 16-17 0.532320980959291 1102 18-19 0.4524969414989073 1102 20-21 0.23009399702319655 1102 22-23 0.35919427474197363 1102 24-25 0.42440499291544853 1102 26-27 0.5559915322838052 1102 28-29 0.4253300738409962 1102 30-31 0.6251979663098055 1102 32-33 0.6777615566122677 1102 34-35 0.7477191805835801 1102 36-37 0.5114932407345378 1102 38-39 0.4886650556685055 1102 40-41 0.7099582796937973 1102 42-43 0.600321321226609 1102 44-45 0.6891761972918182 1102 46-47 0.554578304127606 1102 48-49 0.7332966975566642 1102 50-51 0.6868409690755151 1102 52-53 0.9633106650565431 1102 54-55 0.9025354700869066 1102 56-57 0.9533118259726265 1102 58-59 0.9180540588878578 1102 60-61 0.8255245692937017 1102 62-63 0.7931020133901718 1102 64-65 0.6120085530762829 1102 66-67 0.6107423925836883 1102 68-69 0.667534932125708 1102 70-71 0.6850224908619751 1102 72-73 0.6750832242520701 1102 74-75 0.7569445859253072 1102 76-77 0.8829944787400414 1102 78-79 0.8067202894388217 1102 80-81 0.7699857634991538 1102 82-83 0.5663956752696606 1102 84-85 0.7035617101548866 1102 86-87 0.5216897862149708 1102 88-89 0.4221634752005201 1102 90-91 0.472217850635289 1102 92-93 0.7141475794107919 1102 94-95 0.6883026512279748 1102 96-97 0.47392752356245893 1102 98-99 0.7204173641243976 1102 100-101 0.8813592798628278 1102 102-103 0.6136310466229062 1102 104-105 0.43057955444615814 1102 106-107 0.29633633599899767 1102 108-109 0.5942523120033023 1102 110-111 0.6270079824147032 1102 112-113 0.41319263234144543 1102 114-115 0.6751327104261584 1102 116-117 0.757588306651364 1102 118-119 -0.013128672110642725 1102 120-121 -0.1712065954969333 1102 122-123 -0.4837152522528321 1102 124-125 -0.5157843143832928 1103 1 0.35185065432785834 1103 2 0.18727747727675492 1103 3 0.3537856787678848 1103 4 0.42711432192042764 1103 5 0.20763881952517593 1103 6 0.4029854838074094 1103 7 0.30807730568241 1103 8 0.4126437628249704 1103 9 0.465321425563495 1103 10-11 0.4990558238999512 1103 12-13 0.17346359315205717 1103 14-15 0.4488116372640363 1103 16-17 0.46032037039655904 1103 18-19 0.43980741264982015 1103 20-21 0.6447195185455357 1103 22-23 0.8128449310969188 1103 24-25 0.8739928630691125 1103 26-27 0.8949861389796467 1103 28-29 0.7561685799975066 1103 30-31 0.9342470148495465 1103 32-33 0.8119581578130379 1103 34-35 0.6705898430441408 1103 36-37 0.7583768267730022 1103 38-39 0.6614390456959853 1103 40-41 0.6948341319376148 1103 42-43 0.5724516763705978 1103 44-45 0.6769674865967907 1103 46-47 0.6593737656112744 1103 48-49 0.4082865215110765 1103 50-51 0.34800663509770047 1103 52-53 0.586094831129607 1103 54-55 0.5852489126431308 1103 56-57 0.6102620651096977 1103 58-59 0.52670369763824 1103 60-61 0.4707036676960641 1103 62-63 0.2794152320733936 1103 64-65 0.2713934111204459 1103 66-67 0.3789218980278761 1103 68-69 0.3386171545740666 1103 70-71 0.34129602296739847 1103 72-73 0.278461671387511 1103 74-75 0.2956262792192952 1103 76-77 0.34291357917760834 1103 78-79 0.3441096249430444 1103 80-81 0.49120536256295466 1103 82-83 0.49734357391624684 1103 84-85 0.5228249644542657 1103 86-87 0.5874677449002235 1103 88-89 0.5221634752005215 1103 90-91 0.4036720075499147 1103 92-93 0.5260662728065384 1103 94-95 0.6687010434127707 1103 96-97 0.45820807713933576 1103 98-99 0.6875080326905909 1103 100-101 0.5417999026368179 1103 102-103 0.6808972519755088 1103 104-105 0.40747229892565073 1103 106-107 0.27073594930926603 1103 108-109 0.34748575048910624 1103 110-111 0.35081992909222137 1103 112-113 0.529670194160925 1103 114-115 0.27887495119139416 1103 116-117 0.2569624798476653 1103 118-119 0.6179082669348439 1103 120-121 0.5487028376973271 1103 122-123 0.35303553639070273 1103 124-125 0.3944527874789223 1104 1 0.19846260184132802 1104 2 0.1788926890020015 1104 3 0.2587796736149137 1104 4 0.18851539138584528 1104 5 0.08129139775413208 1104 6 0.28194302912015345 1104 7 0.10153991176929367 1104 8 0.07153612357269523 1104 9 0.0281637317415786 1104 10-11 0.0905210313891942 1104 12-13 0.20518749565428607 1104 14-15 0.38861184756897416 1104 16-17 0.3861430265180701 1104 18-19 0.1352900923932694 1104 20-21 0.1307195384796529 1104 22-23 0.042842289826310775 1104 24-25 -0.09539829913979503 1104 26-27 0.1015222870103969 1104 28-29 0.21090567390215398 1104 30-31 0.13605130168156876 1104 32-33 -4.094671863370536E-4 1104 34-35 -0.005124421312153515 1104 36-37 0.1156248719835844 1104 38-39 0.009541068510586115 1104 40-41 0.07893218786278666 1104 42-43 0.10994650595880984 1104 44-45 -0.04188047090367064 1104 46-47 0.114465935988175 1104 48-49 0.05351404454744113 1104 50-51 0.03363936891244279 1104 52-53 -0.14625973702264972 1104 54-55 -0.07106820926894741 1104 56-57 0.04190174596447349 1104 58-59 -0.04781252624183452 1104 60-61 0.05759025751670421 1104 62-63 0.014067207295287432 1104 64-65 -0.050353216276008084 1104 66-67 -0.047931102804376735 1104 68-69 0.15879646196314567 1104 70-71 0.03562127799951753 1104 72-73 -0.0401565454059849 1104 74-75 0.11186610658983298 1104 76-77 0.10367301922824268 1104 78-79 0.16704108646272076 1104 80-81 0.22719541543819943 1104 82-83 0.376074368639685 1104 84-85 0.24495955961725713 1104 86-87 0.2068220289913043 1104 88-89 0.08732935197777181 1104 90-91 0.3087158376912953 1104 92-93 0.09124460840737925 1104 94-95 0.32929154181555376 1104 96-97 0.17500406639484822 1104 98-99 0.022748811915253242 1104 100-101 0.3874923889694877 1104 102-103 0.29512597604246693 1104 104-105 0.3133994596594256 1104 106-107 0.47186123025574034 1104 108-109 0.4006899107394801 1104 110-111 0.29132281704752927 1104 112-113 0.3212278461211966 1104 114-115 0.25790675109272243 1104 116-117 0.04232560471995939 1104 118-119 -0.13448881072349295 1104 120-121 0.03664643411118007 1104 122-123 0.0463005455354768 1104 124-125 -0.03934849926837458 1105 1 0.022016579208965936 1105 2 -0.011193840877773198 1105 3 0.2826171520779006 1105 4 0.2970953563538892 1105 5 -0.03045528399441011 1105 6 -0.026989641756077987 1105 7 -0.03683391831141236 1105 8 0.12683076902928292 1105 9 0.0924860257140736 1105 10-11 -0.041169761507042324 1105 12-13 0.06881941195941721 1105 14-15 0.23805797042899712 1105 16-17 0.3345186225146932 1105 18-19 0.2695128268901996 1105 20-21 0.12906339571728864 1105 22-23 0.26303649284936625 1105 24-25 0.14210307302460734 1105 26-27 0.1349458252377076 1105 28-29 0.06997484530576514 1105 30-31 0.21328148643455336 1105 32-33 0.3344714289443971 1105 34-35 0.3297433497678668 1105 36-37 0.25027357403538986 1105 38-39 0.38914259197101586 1105 40-41 0.46861971620095844 1105 42-43 0.3842970545859288 1105 44-45 0.39201461460006826 1105 46-47 0.41911148802781284 1105 48-49 0.23832788360090973 1105 50-51 0.32360395513925155 1105 52-53 0.46260897906102727 1105 54-55 0.6283979591230633 1105 56-57 0.49563713539665954 1105 58-59 0.4439628397084405 1105 60-61 0.27436240186363037 1105 62-63 0.3142013214498185 1105 64-65 0.16057350775906087 1105 66-67 0.24303456683571056 1105 68-69 0.42795545644107946 1105 70-71 0.3568234849171361 1105 72-73 0.28478988052339105 1105 74-75 0.28348926967932897 1105 76-77 0.39019406942320956 1105 78-79 0.4843857216579046 1105 80-81 0.4372964544649456 1105 82-83 0.35289552908507815 1105 84-85 0.5249192776434377 1105 86-87 0.4923992687215488 1105 88-89 0.47155924559326934 1105 90-91 0.5275438422540404 1105 92-93 0.6873641275055178 1105 94-95 0.6350061888948701 1105 96-97 0.583597633688175 1105 98-99 0.6092488620290837 1105 100-101 0.6791811295850749 1105 102-103 0.7351421079229752 1105 104-105 0.6036158082225924 1105 106-107 0.6321491222771414 1105 108-109 0.6851133391936344 1105 110-111 0.6378694968869745 1105 112-113 0.36869160904937104 1105 114-115 0.4898120789087592 1105 116-117 0.30941561393135686 1105 118-119 0.3648656754188373 1105 120-121 0.5995905977590823 1105 122-123 0.5827500045447529 1105 124-125 0.5933863317525621 1106 1 -0.4669320351172921 1106 2 -0.3983694380231597 1106 3 -0.15964971163911557 1106 4 -0.8124899224156792 1106 5 -0.22858461354368131 1106 6 -0.46487518689237817 1106 7 -0.3448057383678247 1106 8 -0.4252393495060929 1106 9 -0.17749474879842353 1106 10-11 -0.26927188764542365 1106 12-13 -0.08597736897029051 1106 14-15 -0.1900317310746047 1106 16-17 -0.14097651008013656 1106 18-19 -0.34401890824274517 1106 20-21 -0.35187895833684735 1106 22-23 -0.12829699742671608 1106 24-25 -0.11467707197306964 1106 26-27 -0.1533623754571991 1106 28-29 -0.2994825687135787 1106 30-31 -0.2256154675681401 1106 32-33 -0.10435686260292698 1106 34-35 -0.13835585549145435 1106 36-37 -0.24327215515988598 1106 38-39 -0.26434197314497254 1106 40-41 -0.3331746626326897 1106 42-43 -0.5715878627793529 1106 44-45 -0.38534900868500443 1106 46-47 -0.3021374711365574 1106 48-49 -0.35839789817018897 1106 50-51 -0.46775917987947224 1106 52-53 -0.5910392860676019 1106 54-55 -0.4674559184528668 1106 56-57 -0.5144813127882522 1106 58-59 -0.44233322408007325 1106 60-61 -0.29907045933358134 1106 62-63 -0.24590557400451374 1106 64-65 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-1.6088947090524677 2216 64-65 -1.3016278105600811 2216 66-67 -1.2907756339817738 2216 68-69 -1.6149646366167438 2216 70-71 -1.4754139417755958 2216 72-73 -1.7614744780077203 2216 74-75 -1.8507619864397107 2216 76-77 -1.8488280156536128 2216 78-79 -2.0113450892053066 2216 80-81 -1.8310061288589665 2216 82-83 -2.0233403951115676 2216 84-85 -1.9647106721117993 2216 86-87 -2.1649972933089217 2216 88-89 -2.199093209291455 2216 90-91 -2.167548407774234 2216 92-93 -2.0974618737546393 2216 94-95 -1.9911004883269676 2216 96-97 -2.007610340850171 2216 98-99 -2.072712703151783 2216 100-101 -2.146517207975709 2216 102-103 -2.4928636920350193 2216 104-105 -2.825169108655448 2216 106-107 -2.684615018149696 2216 108-109 -2.6048118591196925 2216 110-111 -2.875208681376918 2216 112-113 -3.1212432827870664 2216 114-115 -3.130741017364077 2216 116-117 -3.1071736391826867 2216 118-119 -3.006452805282972 2216 120-121 -2.623069628100005 2216 122-123 -2.172886375247483 2216 124-125 -1.9190920597688361 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35333.0 3 3910.0 4 1975.0 5 4230.0 6 11511.0 7 9631.0 8 5664.0 9 5665.0 10 4139.0 11 2692.0 12 1932.0 13 1709.0 14 1776.0 15 1858.0 16 1562.0 17 1577.0 18 1670.0 19 1907.0 20 2233.0 21 2685.0 22 3123.0 23 3683.0 24 4020.0 25 4474.0 26 5014.0 27 5779.0 28 6671.0 29 8344.0 30 10490.0 31 14092.0 32 19322.0 33 26458.0 34 39159.0 35 63406.0 36 104886.0 37 36769.0 38 4627.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.41877663087822 18.9942108194216 11.724062010283243 13.862950539416937 2 20.45893016889234 24.59792414395189 34.0423146980524 20.900830989103373 3 30.991061286338446 24.463235309966656 24.29079810405292 20.254905299641976 4 16.475495532394184 23.419092682611367 28.371506483148128 31.73390530184632 5 16.02444176222088 36.06129838779205 24.741572549357706 23.172687300629363 6 30.917845242662366 37.162968933871866 9.913401066429874 22.005784757035894 7 28.629159141166177 41.69270956812608 11.965869071378386 17.712262219329364 8 34.0024052968257 30.739305481317995 10.48351638877873 24.774772833077574 9 35.44543060231304 18.840764001741462 14.724189508985338 30.98961588696016 10-11 30.346913000185356 28.41233851605033 15.015905926574105 26.224842557190208 12-13 32.27200427606481 30.067804939027283 16.854031872200835 20.80615891270707 14-15 35.05090758613555 24.273133639957067 20.13964024156318 20.536318532344204 16-17 29.800443124458486 24.770678782183637 21.358771321054014 24.07010677230386 18-19 28.470099875425127 25.614037734547757 21.1161951644259 24.799667225601212 20-21 37.420200957804035 23.21812672152559 17.950312299290054 21.411360021380325 22-23 33.44253391324589 26.268702395372195 16.851337778946416 23.437425912435504 24-25 26.799977585144124 28.560513645043518 19.152093418222414 25.48741535158995 26-27 23.853548143757745 28.712646155504068 21.222371895037448 26.21143380570074 28-29 24.884037728691393 27.827604193609574 22.735394789026262 24.55296328867277 30-31 26.041644215889548 28.884990236606043 19.62011129838053 25.45325424912388 32-33 23.318346990590072 29.12153162434426 20.786222622624347 26.773898762441323 34-35 22.77414015319692 29.691601756979857 21.474617330994143 26.059640758829083 36-37 22.894404476074296 30.445840104487715 19.910750078667522 26.749005340770466 38-39 23.356064296151974 28.530339800593996 21.09690545672424 27.01669044652979 40-41 22.33564953510727 28.30759217031842 22.66282021992422 26.69393807465009 42-43 21.67214824840833 28.717740927371555 24.159658432080832 25.450452392139283 44-45 21.97604196750708 28.298001198332678 24.117953868502386 25.608002965657857 46-47 19.96614063597843 28.04378655884546 24.145649147157837 27.84442365801827 48-49 19.417084430727318 28.732935613326493 25.110673350891638 26.73930660505455 50-51 20.048364362103378 28.167714848548854 24.081637491432783 27.70228329791499 52-53 20.68686446340128 26.808059864907385 23.204225200332772 29.300850471358558 54-55 19.877666613503227 26.543176616132662 23.061653785308785 30.517502985055327 56-57 20.618114534064837 25.935528923402206 24.097069780624192 29.349286761908765 58-59 22.106005026100377 24.194897171848666 23.705111018194827 29.99398678385613 60-61 22.11441059705417 23.988098573639267 23.39572134885706 30.501769480449504 62-63 22.469707615447284 23.449064395294606 23.578273107776663 30.502954881481447 64-65 22.899100800055177 22.763100041382163 23.77868732326367 30.559111835298985 66-67 23.77811831189908 24.073391568978636 23.059117154514126 29.089372964608156 68-69 23.46690462488172 24.107063953425715 23.236490374978178 29.18954104671439 70-71 23.189168098402853 23.42597043689331 24.19684450374982 29.188016960954023 72-73 24.55132474239025 22.408761641202045 24.751657948562105 28.2882556678456 74-75 24.90152229556783 21.2017782567695 25.303408325474873 28.593291122187797 76-77 24.788512312085782 20.97246618891104 25.942346031574974 28.2966754674282 78-79 25.56460903309794 21.56037601447503 26.63417903901425 26.240835913412784 80-81 26.48235996491175 22.420270145029992 25.62778437538391 25.469585514674346 82-83 25.626215036057744 21.813426498670196 26.25339994568708 26.306958519584978 84-85 25.476511774292295 21.733830288084295 26.810658515344088 25.978999422279326 86-87 26.06171572916981 21.837699314173882 26.34568050245061 25.754904454205697 88-89 26.38081113357793 21.553091870125503 25.95722298628566 26.108874010010908 90-91 27.121064190681864 21.578548074581285 25.505790157701785 25.794597577035066 92-93 27.305070405837245 22.504863472492413 25.19779917765551 24.99226694401483 94-95 26.408347101826497 23.215242233869308 24.64425554754414 25.73215511676005 96-97 25.71171769250002 23.920784225818412 24.016592376731875 26.350905704949696 98-99 25.181641639527292 25.309022486706915 23.587225705498067 25.922110168267725 100-101 24.93623019418135 26.93009406787241 23.35596752831789 24.77770820962835 102-103 24.28983701813448 28.56234562845652 23.357572988374447 23.79024436503455 104-105 23.274422113260414 30.408211649334554 23.068592057761734 23.2487741796433 106-107 22.791495634987776 31.301356650756993 22.816604827646405 23.090542886608826 108-109 22.574669488639547 32.76135937479794 22.714115755488407 21.9498553810741 110-111 22.64385380341309 34.68591183202652 21.425369306303317 21.244865058257073 112-113 22.670416888931996 35.577647382118556 21.029694647850896 20.722241081098556 114-115 22.434505405210253 36.00222437522969 21.496150990245727 20.067119229314333 116-117 22.301488432641204 36.513800223286445 21.007784851867978 20.176926492204373 118-119 22.82229852433064 36.76340547045858 20.735828872391913 19.67846713281887 120-121 23.04316761518243 36.17374380483242 20.976225859187167 19.806862720797984 122-123 23.785763243769857 36.50426550908064 20.621333149407054 19.08863809774245 124-125 24.764051837840935 37.7338822317018 19.254255816741978 18.247810113715293 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.0 3 2.5 4 2.5 5 3.0 6 5.0 7 4.5 8 8.0 9 10.0 10 11.5 11 14.0 12 17.5 13 28.0 14 37.5 15 52.5 16 76.0 17 108.0 18 142.5 19 183.5 20 269.0 21 379.0 22 530.0 23 775.5 24 1012.5 25 1260.0 26 1611.0 27 2008.5 28 2380.0 29 2703.5 30 3081.0 31 3496.5 32 3793.0 33 3939.0 34 4103.0 35 4249.0 36 4312.0 37 4302.5 38 4113.5 39 3860.5 40 3662.0 41 3437.5 42 3227.5 43 3147.5 44 3160.5 45 3452.0 46 4222.0 47 5385.5 48 6692.5 49 9410.0 50 15108.5 51 24000.5 52 34890.0 53 43527.0 54 45860.0 55 42408.5 56 35968.5 57 28211.5 58 20994.0 59 15337.0 60 11127.0 61 8085.0 62 6229.5 63 5290.0 64 4530.0 65 3890.5 66 3520.0 67 3417.0 68 3147.5 69 2487.5 70 1663.0 71 1200.0 72 1069.5 73 926.0 74 677.0 75 428.5 76 300.0 77 242.5 78 179.5 79 140.5 80 109.0 81 75.0 82 63.5 83 44.5 84 25.5 85 21.5 86 21.0 87 16.5 88 17.0 89 13.0 90 8.5 91 6.5 92 5.0 93 3.0 94 0.5 95 1.5 96 2.0 97 1.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15086922224760657 2 0.01573350460582183 3 0.00883662587450267 4 0.017888779209359065 5 0.003879494286367026 6 0.0 7 0.0 8 4.3105492070744735E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.46582381061171E-4 28-29 0.007327933652026604 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.388186508843092E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0021552746035372367 66-67 2.1552746035372367E-4 68-69 0.006681351270965434 70-71 0.019181943971481406 72-73 2.1552746035372367E-4 74-75 0.008728862144325809 76-77 6.46582381061171E-4 78-79 0.0014009284922992039 80-81 0.0010776373017686183 82-83 0.0 84-85 0.018535361590420237 86-87 0.002586329524244684 88-89 0.01810430666971279 90-91 2.1552746035372367E-4 92-93 0.013039411351400281 94-95 0.012177301509985387 96-97 0.0064658238106117105 98-99 0.0032329119053058552 100-101 9.698735715917565E-4 102-103 0.0 104-105 6.46582381061171E-4 106-107 9.698735715917565E-4 108-109 0.0 110-111 0.0 112-113 0.00118540103194548 114-115 0.0059270051597274005 116-117 0.0 118-119 0.06983089715460647 120-121 0.0016164559526529276 122-123 0.005172659048489368 124-125 0.23955877218316385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 463978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.76593898254177 #Duplication Level Percentage of deduplicated Percentage of total 1 84.5369854670503 52.2150628612585 2 7.442583531295052 9.193963205328808 3 2.6092825622676132 4.834943625876949 4 1.4332739399062417 3.5411004287006462 5 0.9018449419662514 2.7851649828600706 6 0.611651719546483 2.2667545672844893 7 0.4393962866244657 1.899780696016153 8 0.33203162476551895 1.6406596060432999 9 0.26073407855780756 1.4494036668183714 >10 1.366012364220807 14.229952778682172 >50 0.04773619625682267 2.003238533633016 >100 0.0153313330542797 1.8900344604317105 >500 0.0010453181627917978 0.37469255244951133 >1k 0.0020906363255835956 1.6752480346163101 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1608 0.3465681562487877 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1597 0.34419735418489666 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1394 0.30044527973309076 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 1110 0.23923548099263328 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 1068 0.23018332765777685 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 1007 0.21703615257619974 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 604 0.1301785860536491 No Hit GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT 589 0.12694567414834323 No Hit GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT 548 0.11810904827384058 No Hit ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA 480 0.10345318096978737 No Hit GTGGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCC 464 0.10000474160412778 No Hit GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT 464 0.10000474160412778 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.1552746035372367E-4 0.0 0.0 0.0 0.0 3 2.1552746035372367E-4 0.0 0.0 2.1552746035372367E-4 0.0 4 2.1552746035372367E-4 0.0 0.0 2.1552746035372367E-4 0.0 5 0.0028018569845984077 0.0 0.0 2.1552746035372367E-4 0.0 6 0.0036639668260133023 0.0 0.0 2.1552746035372367E-4 0.0 7 0.004526076667428197 0.0 0.0 2.1552746035372367E-4 0.0 8 0.004957131588135644 0.0 0.0 2.1552746035372367E-4 0.0 9 0.004957131588135644 0.0 0.0 2.1552746035372367E-4 0.0 10-11 0.00883662587450267 0.0 0.0 4.3105492070744735E-4 0.0 12-13 0.01465586730405321 0.0 0.0 4.3105492070744735E-4 0.0 14-15 0.01584126833599869 0.0 0.0 0.0010776373017686183 0.0 16-17 0.017565488018828478 0.0 0.0 0.0016164559526529276 0.0 18-19 0.02058287246378061 0.0 0.0 0.0019397471431835131 0.0 20-21 0.023600256908732742 0.0 0.0 0.003340675635482717 0.0 22-23 0.02888067968739897 0.0 0.0 0.016703378177413585 0.0 24-25 0.03222135532288169 0.0 0.0 0.04030363508614633 0.0 26-27 0.03685519572048675 0.0 0.0 0.1106733508916371 0.0 28-29 0.04310549207074473 0.0 0.0 0.3693063033161055 0.0 30-31 0.04946355215117958 0.0 0.0 0.9145907780110263 0.0 32-33 0.051403299294363096 0.0 0.0 1.697817568936458 0.0 34-35 0.05334304643754661 0.0 0.0 2.845285767859683 0.0 36-37 0.06304178215346418 0.0 0.0 4.3741298078788216 0.0 38-39 0.06821444120195355 0.0 0.0 6.448797141243766 0.0 40-41 0.07403368263150409 0.0 0.0 8.753647802266487 0.0 42-43 0.08718085771308122 0.0 0.0 10.607291725038687 0.0 44-45 0.09203022557104001 0.0 0.0 12.221269111897547 0.0 46-47 0.09300009914263177 0.0 0.0 14.165326804288135 0.0 48-49 0.09321562660298549 0.0 0.0 16.310902672109453 0.0 50-51 0.09353891779351607 0.0 0.0 18.310027630620418 0.0 52-53 0.09353891779351607 0.0 0.0 20.1567746746613 0.0 54-55 0.09418550017457725 0.0 0.0 21.933367530357042 0.0 56-57 0.09558642866687644 0.0 1.0776373017686184E-4 23.768476091538822 0.0 58-59 0.09644853850829134 0.0 2.1552746035372367E-4 25.613714443357228 0.0 60-61 0.09709512088935252 0.0 2.1552746035372367E-4 27.500549595023905 0.0 62-63 0.09752617581005996 0.0 2.1552746035372367E-4 29.220889783567323 0.0 64-65 0.09828052192129799 0.0 2.1552746035372367E-4 30.875278569242507 0.0 66-67 0.09838828565147485 0.0 2.1552746035372367E-4 32.70252037812138 0.0 68-69 0.09881934057218231 0.0 2.1552746035372367E-4 34.626641780429246 0.0 70-71 0.09935815922306661 0.0 2.1552746035372367E-4 36.71596498109824 0.0 72-73 0.09978921414377406 0.0 2.1552746035372367E-4 38.79796024811522 0.0 74-75 0.1002202690644815 0.0 2.1552746035372367E-4 40.60138627262499 0.0 76-77 0.10043579652483522 0.0 2.1552746035372367E-4 42.513330373422875 0.0 78-79 0.10043579652483522 0.0 2.1552746035372367E-4 44.38173792722931 0.0 80-81 0.10065132398518896 0.0 2.1552746035372367E-4 46.166197535227965 0.0 82-83 0.10065132398518896 0.0 2.1552746035372367E-4 48.062084840229495 0.0 84-85 0.10065132398518896 2.1552746035372367E-4 2.1552746035372367E-4 50.1141217902573 0.0 86-87 0.10065132398518896 2.1552746035372367E-4 2.1552746035372367E-4 51.77605403704486 0.0 88-89 0.10065132398518896 2.1552746035372367E-4 2.1552746035372367E-4 53.24929199229274 0.0 90-91 0.10065132398518896 2.1552746035372367E-4 2.1552746035372367E-4 55.11532874403528 0.0 92-93 0.10065132398518896 2.1552746035372367E-4 2.1552746035372367E-4 56.7215471423214 0.0 94-95 0.10065132398518896 2.1552746035372367E-4 2.1552746035372367E-4 58.13573057343236 0.0 96-97 0.10065132398518896 2.1552746035372367E-4 2.1552746035372367E-4 59.48816538715198 0.0 98-99 0.10065132398518896 2.1552746035372367E-4 2.1552746035372367E-4 60.702123807594326 0.0 100-101 0.10075908771536582 2.1552746035372367E-4 2.1552746035372367E-4 61.86457116501214 0.0 102-103 0.10086685144554268 2.1552746035372367E-4 2.1552746035372367E-4 62.892313859708864 0.0 104-105 0.10086685144554268 2.1552746035372367E-4 2.1552746035372367E-4 63.88460228717741 0.0 106-107 0.10086685144554268 2.1552746035372367E-4 2.1552746035372367E-4 64.79488251598136 0.0 108-109 0.10086685144554268 2.1552746035372367E-4 2.1552746035372367E-4 65.66205725271456 0.0 110-111 0.10086685144554268 2.1552746035372367E-4 2.1552746035372367E-4 66.54550431270448 0.0 112-113 0.10086685144554268 2.1552746035372367E-4 2.1552746035372367E-4 67.41203246705663 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAG 60 3.1089257E-7 59.610474 1 ATCAAGG 35 0.007799341 50.99007 8 TAACAAC 275 0.0 47.68838 1 AGTCGTT 50 5.334415E-4 47.590733 7 GTGGAAT 215 0.0 47.133865 1 TCCTAGG 65 3.5164925E-5 45.770187 2 TGTATGG 110 9.607902E-9 43.27363 2 ACCACGT 55 8.531323E-4 43.2643 6 GATTGTG 55 8.531323E-4 43.2643 9 TGGAATC 250 0.0 42.840893 2 GTCCACG 105 3.2110438E-7 39.74032 1 AGTCGTG 80 1.19774064E-4 37.18026 7 GTGTAAG 130 4.125468E-8 36.68337 1 TATATGG 130 4.1927706E-8 36.61615 2 GCAGAGA 640 0.0 36.25857 3 ACAAAGT 115 6.656246E-7 36.210342 9 GAGTATA 250 0.0 35.766285 1 CCTAGGG 100 1.0606724E-5 35.700745 3 TAGGGCA 85 1.7097879E-4 34.996956 5 AGTATAT 120 9.2710616E-7 34.709057 2 >>END_MODULE