FastQCFastQC Report
Thu 26 May 2016
SRR935984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463978
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT73811.5908081848708344No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT41650.8976718723732591RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT23650.5097224437365565RNA PCR Primer, Index 46 (95% over 23bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA16690.35971533133036476No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT14450.3114371802111307No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT13830.29807447766919987No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC11670.2515205462327955No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA11290.24333050273935403No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC9780.21078585622594176No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC7140.15388660669255871No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA6550.14117048653168898No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT5570.12004879541702407No Hit
GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT5230.11272086176499747No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA5040.10862584001827673No Hit
GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.10862584001827673No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT4880.10517740065261715No Hit
GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT4840.10431529081120225No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG4840.10431529081120225TruSeq Adapter, Index 4 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATT300.004207457459.597972
TATAACG300.004207457459.597972
TAGGGTC300.00423089259.5144425
CTCGGAG453.1433144E-452.9759753
CTATACA708.9059176E-751.1834341
CTACGGG709.026244E-751.0839733
TCTACGG751.4523448E-647.6783752
GTTGTAG505.331413E-447.596168
GTTCAAG853.391593E-642.1510661
GTCTATG600.001284821139.8093381
AGTTGTA758.184984E-539.663477
GAATGGT300.001308900439.66346750-51
GTCTACG957.2809944E-637.7141151
CTATGGG650.001922118436.675683
TCGGAGA650.001922118436.675684
TGCGAGG650.001922118436.675682
CGTAATG654.2042302E-836.61243426-27
AGTAGCG650.001938476236.6124347
CTTTACA1407.7980076E-834.1222881
TCTATGG700.00276488634.0559842
Produced by FastQC (version 0.11.5)