##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935984_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 463978 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.311751850303246 33.0 31.0 34.0 30.0 34.0 2 32.41511019919048 34.0 31.0 34.0 31.0 34.0 3 32.40934699490062 34.0 31.0 34.0 30.0 34.0 4 35.893014323955015 37.0 35.0 37.0 35.0 37.0 5 35.88797960248115 37.0 35.0 37.0 35.0 37.0 6 35.979117544366325 37.0 35.0 37.0 35.0 37.0 7 35.96330429460018 37.0 35.0 37.0 35.0 37.0 8 35.98743690433598 37.0 35.0 37.0 35.0 37.0 9 37.65584144075797 39.0 37.0 39.0 35.0 39.0 10-11 37.59325334390854 39.0 37.0 39.0 35.0 39.0 12-13 37.55898447771231 39.0 37.0 39.0 35.0 39.0 14-15 38.65953342615383 40.0 38.0 41.0 34.0 41.0 16-17 38.62852010224623 40.0 38.0 41.0 34.5 41.0 18-19 38.722014836910375 40.0 38.0 41.0 34.5 41.0 20-21 38.82608658169137 40.0 38.0 41.0 35.0 41.0 22-23 38.656360861937415 40.0 38.0 41.0 34.5 41.0 24-25 38.352681592661725 40.0 38.0 41.0 34.0 41.0 26-27 38.19242830479031 40.0 37.0 41.0 33.0 41.0 28-29 38.04154938380699 40.0 37.0 41.0 33.0 41.0 30-31 37.820412821297566 40.0 36.5 41.0 33.0 41.0 32-33 37.36051709348288 40.0 35.0 41.0 33.0 41.0 34-35 37.023847035850835 39.0 35.0 41.0 32.0 41.0 36-37 36.75383100060779 39.0 35.0 41.0 32.0 41.0 38-39 36.19474630262642 39.0 35.0 41.0 30.5 41.0 40-41 35.84354969416654 39.0 35.0 41.0 30.0 41.0 42-43 35.411910694041524 38.5 35.0 41.0 28.5 41.0 44-45 34.80448103142821 38.0 35.0 41.0 26.5 41.0 46-47 34.07699071938756 37.5 34.5 40.0 22.0 41.0 48-49 33.15397820586321 37.0 34.0 40.0 7.5 41.0 50-51 32.17131954532327 36.0 33.0 39.0 2.0 41.0 52-53 31.619424843419303 35.5 33.0 39.0 2.0 41.0 54-55 31.17667432507576 35.0 33.0 38.5 2.0 41.0 56-57 30.822309462948674 35.0 32.0 37.0 2.0 40.0 58-59 30.548308324963685 35.0 32.0 36.5 2.0 39.5 60-61 30.207277069171383 35.0 32.0 36.0 2.0 39.0 62-63 29.92020419071594 35.0 32.0 35.5 2.0 39.0 64-65 29.668579113664872 35.0 31.5 35.0 2.0 37.0 66-67 29.276226459013145 35.0 31.0 35.0 2.0 37.0 68-69 28.81084016914595 35.0 31.0 35.0 2.0 36.0 70-71 28.3562022337266 35.0 29.5 35.0 2.0 36.0 72-73 28.057054429304838 35.0 29.0 35.0 2.0 35.5 74-75 27.919823784748417 35.0 29.0 35.0 2.0 35.0 76-77 27.783016651651586 35.0 29.0 35.0 2.0 35.0 78-79 27.794185284647117 35.0 29.0 35.0 2.0 35.0 80-81 27.72431774782425 35.0 29.0 35.0 2.0 35.0 82-83 27.600648091073282 35.0 29.0 35.0 2.0 35.0 84-85 27.545088344706 35.0 29.0 35.0 2.0 35.0 86-87 27.516028777226506 35.0 29.0 35.0 2.0 35.0 88-89 27.430731629516917 35.0 28.0 35.0 2.0 35.0 90-91 27.308234442150273 35.0 27.0 35.0 2.0 35.0 92-93 27.221008323670517 35.0 27.0 35.0 2.0 35.0 94-95 27.194564181922416 34.5 27.0 35.0 2.0 35.0 96-97 27.145875450991213 34.0 27.0 35.0 2.0 35.0 98-99 27.05820319066852 34.0 27.0 35.0 2.0 35.0 100-101 26.94437559539461 34.0 25.5 35.0 2.0 35.0 102-103 26.201086042872724 33.0 24.5 35.0 2.0 35.0 104-105 26.618032536025414 34.0 25.0 35.0 2.0 35.0 106-107 26.49060515800318 34.0 24.0 35.0 2.0 35.0 108-109 26.17744806865843 34.0 21.5 35.0 2.0 35.0 110-111 25.74350939053145 34.0 17.0 35.0 2.0 35.0 112-113 25.179502045355598 34.0 2.0 35.0 2.0 35.0 114-115 24.477868562733576 34.0 2.0 35.0 2.0 35.0 116-117 23.80918168533853 33.5 2.0 35.0 2.0 35.0 118-119 23.221611800559508 33.0 2.0 35.0 2.0 35.0 120-121 22.571199496527854 33.0 2.0 35.0 2.0 35.0 122-123 21.84820077676097 33.0 2.0 35.0 2.0 35.0 124-125 19.738241899400403 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03361584080085578 1101 2 0.11893445749972642 1101 3 0.15829970537505034 1101 4 0.16775037653575708 1101 5 0.17468799415782144 1101 6 0.29650846895437155 1101 7 0.24721424398299519 1101 8 0.1625938063044785 1101 9 0.24313707512327198 1101 10-11 0.2089602969088702 1101 12-13 0.17113738615403662 1101 14-15 0.4098689931764241 1101 16-17 0.3433298918800318 1101 18-19 0.3231453876179984 1101 20-21 0.38575053911293367 1101 22-23 0.14007606434824282 1101 24-25 0.18215897082544785 1101 26-27 0.27396495802477716 1101 28-29 0.2517616908302358 1101 30-31 0.35034691237971316 1101 32-33 0.4369254707067043 1101 34-35 0.5912271740047927 1101 36-37 0.3947550029929374 1101 38-39 0.34370733840965784 1101 40-41 0.4356721092411888 1101 42-43 0.5013850785160514 1101 44-45 0.6112127779800645 1101 46-47 0.7243860220684368 1101 48-49 0.4370610274955098 1101 50-51 0.6780468350296616 1101 52-53 1.0611057811671287 1101 54-55 0.941415057204253 1101 56-57 0.6992213562176275 1101 58-59 0.8626949443253231 1101 60-61 0.8485109809609526 1101 62-63 0.7691145962499952 1101 64-65 0.8102707325139775 1101 66-67 0.4659384924296752 1101 68-69 0.84308253531605 1101 70-71 0.7182793226546345 1101 72-73 0.6201267679218212 1101 74-75 0.6462333244891454 1101 76-77 0.7519650571155694 1101 78-79 0.7024833463463693 1101 80-81 0.8338783310348248 1101 82-83 0.7706910575815868 1101 84-85 0.787592574487654 1101 86-87 0.7139890266987585 1101 88-89 0.6366072502310658 1101 90-91 0.6155298906238968 1101 92-93 0.6816067637694943 1101 94-95 0.600757871080905 1101 96-97 0.8212624088550875 1101 98-99 0.5298769069617393 1101 100-101 0.6698274544648122 1101 102-103 0.3794060494291429 1101 104-105 0.568580042609053 1101 106-107 0.5374984938976013 1101 108-109 0.7474225604086548 1101 110-111 0.554011330706075 1101 112-113 0.4686652707171213 1101 114-115 0.3894399648185711 1101 116-117 0.36947434919955313 1101 118-119 -0.11639772651541946 1101 120-121 0.0778634341392177 1101 122-123 -0.12781555237964426 1101 124-125 -0.2513129861806185 1102 1 0.09798607908906831 1102 2 0.06772140297981366 1102 3 0.09505066447160715 1102 4 0.10868894032243048 1102 5 0.19912469378498798 1102 6 0.22130449208075476 1102 7 0.13882552620053445 1102 8 0.07313036004090634 1102 9 0.21764672012246677 1102 10-11 0.09889113388946669 1102 12-13 0.19640215083767032 1102 14-15 0.19227835179749064 1102 16-17 0.23328234230420009 1102 18-19 0.18092757814049776 1102 20-21 0.25378965944577914 1102 22-23 0.18462947657064177 1102 24-25 0.08423386140739098 1102 26-27 0.1796114701553222 1102 28-29 0.2143974502726067 1102 30-31 0.3075617500087233 1102 32-33 0.3436350940924555 1102 34-35 0.38476610522000954 1102 36-37 0.4196807067806887 1102 38-39 0.303317215753367 1102 40-41 0.2263783285095755 1102 42-43 0.07427776776877693 1102 44-45 0.009198675208132556 1102 46-47 0.17920176746207517 1102 48-49 0.2705172872891026 1102 50-51 0.3479946925970694 1102 52-53 0.5269052084108807 1102 54-55 0.5309784656443028 1102 56-57 0.7047017150007946 1102 58-59 0.6807462222201721 1102 60-61 0.7072157953555092 1102 62-63 0.7031841375046568 1102 64-65 0.8749057354858252 1102 66-67 0.7810827621654504 1102 68-69 0.38056564981327057 1102 70-71 0.3061703908990836 1102 72-73 0.25196337028849314 1102 74-75 0.3176860180645491 1102 76-77 0.11991367115350471 1102 78-79 0.2233808445902774 1102 80-81 0.23178858121043433 1102 82-83 0.04699326755618927 1102 84-85 0.20881022186431863 1102 86-87 0.09563516059404265 1102 88-89 0.1977300686241037 1102 90-91 0.2987470516980828 1102 92-93 0.3169947250814289 1102 94-95 0.06301404442072212 1102 96-97 0.19210047012920128 1102 98-99 0.22632824969691967 1102 100-101 0.2455569081850264 1102 102-103 0.22247488824688588 1102 104-105 0.27699577719596746 1102 106-107 0.22073997009579216 1102 108-109 0.1827173137452327 1102 110-111 -0.044774263933788916 1102 112-113 0.20738980737892732 1102 114-115 0.1749562559516562 1102 116-117 0.2465019603737062 1102 118-119 -0.035952489578040314 1102 120-121 0.37983160834357577 1102 122-123 0.21958813271248445 1102 124-125 0.08034800695711297 1103 1 0.1282292865786374 1103 2 0.05187729999823176 1103 3 0.09322406428844232 1103 4 0.23777716663768445 1103 5 0.2031187916785484 1103 6 0.2986220864635811 1103 7 0.34127286516461197 1103 8 0.33991574723943785 1103 9 0.4900340204175677 1103 10-11 0.5762290803634684 1103 12-13 0.35002736707863846 1103 14-15 0.343209459968854 1103 16-17 0.4304584896533399 1103 18-19 0.3879872097676511 1103 20-21 0.24025043079003439 1103 22-23 0.5232577456252869 1103 24-25 0.4803415239697202 1103 26-27 0.44242819957427315 1103 28-29 0.5726960154501768 1103 30-31 0.613308671754929 1103 32-33 0.6552027139153935 1103 34-35 0.4600917386788481 1103 36-37 0.4502546357518966 1103 38-39 0.4252771221337497 1103 40-41 0.3967405957325312 1103 42-43 0.5062992392249654 1103 44-45 0.5452956529225972 1103 46-47 0.4685550797649114 1103 48-49 0.35212662889895086 1103 50-51 0.31400924434225885 1103 52-53 0.011279890409454651 1103 54-55 0.04819633478035712 1103 56-57 0.159324183709451 1103 58-59 0.24992552414344615 1103 60-61 0.32311586658782687 1103 62-63 0.3707505362021237 1103 64-65 0.4867710046422289 1103 66-67 0.4653836678335104 1103 68-69 0.23334879828177435 1103 70-71 0.19028049571235428 1103 72-73 0.2307310511677052 1103 74-75 0.14188719848583986 1103 76-77 0.2042858091406785 1103 78-79 0.17346734097778338 1103 80-81 0.06982409560953684 1103 82-83 0.13455559583542254 1103 84-85 0.23720393306814458 1103 86-87 0.21480937449452142 1103 88-89 0.23454700156396768 1103 90-91 0.07064590180493013 1103 92-93 0.3350699260583312 1103 94-95 0.28225949064032463 1103 96-97 0.373554627043827 1103 98-99 0.39019056780010786 1103 100-101 0.12499366406169088 1103 102-103 0.19956097759256863 1103 104-105 0.3090859369598853 1103 106-107 0.34119890975184575 1103 108-109 0.5261334122493544 1103 110-111 0.6885853575173115 1103 112-113 0.621445470348295 1103 114-115 0.7984883613754867 1103 116-117 0.7343772020547874 1103 118-119 0.7341655525507882 1103 120-121 0.6589325547158147 1103 122-123 0.30980895290176136 1103 124-125 0.08130964326524293 1104 1 0.051525293276505124 1104 2 0.11425862970355638 1104 3 -0.011816277382337148 1104 4 0.03736702216517074 1104 5 0.17485470448671947 1104 6 0.2501074274117485 1104 7 0.07479607096942686 1104 8 -0.0165959814970833 1104 9 0.06689250313219475 1104 10-11 0.11838101820889335 1104 12-13 0.13266699311459007 1104 14-15 0.19075208160435153 1104 16-17 0.11300766463003953 1104 18-19 0.3518354413622262 1104 20-21 0.23245245310628349 1104 22-23 0.3387114212274227 1104 24-25 0.2186526556794206 1104 26-27 0.36920021805320147 1104 28-29 0.3477868402741393 1104 30-31 0.3383636151669549 1104 32-33 0.22717609190114985 1104 34-35 0.2666024707094863 1104 36-37 0.5675581974803805 1104 38-39 0.6672523938606929 1104 40-41 0.5812221342477386 1104 42-43 0.2906195605629449 1104 44-45 0.41085871699583976 1104 46-47 0.6188147216484907 1104 48-49 0.3886197690706936 1104 50-51 0.3220289890858865 1104 52-53 0.4595633136954973 1104 54-55 0.40498091175345863 1104 56-57 0.19821315565716446 1104 58-59 0.33823615750020153 1104 60-61 0.3280969042583237 1104 62-63 0.485917409685257 1104 64-65 0.5669609767843014 1104 66-67 0.49362034561344714 1104 68-69 0.3931631119750172 1104 70-71 0.4622678275805292 1104 72-73 0.5015123689125573 1104 74-75 0.5149965720063534 1104 76-77 0.5030546281937092 1104 78-79 0.4706289717879528 1104 80-81 0.6123560275743962 1104 82-83 0.45899831265632685 1104 84-85 0.6091172595242114 1104 86-87 0.6695726319286983 1104 88-89 0.555713047781218 1104 90-91 0.5121853638827645 1104 92-93 0.6284341074388706 1104 94-95 0.5865438308957778 1104 96-97 0.594159278670201 1104 98-99 0.47481089761008377 1104 100-101 0.47522489982748795 1104 102-103 0.550351614537469 1104 104-105 0.536698676854531 1104 106-107 0.49199054340262904 1104 108-109 0.3757815750712261 1104 110-111 0.5615053569192909 1104 112-113 0.6165690867959377 1104 114-115 0.5605522402557881 1104 116-117 0.4495111842436543 1104 118-119 0.5723540894363808 1104 120-121 0.5078331881224045 1104 122-123 0.5883085118594025 1104 124-125 0.726582018066523 1105 1 0.23925885951982906 1105 2 0.16570405574270808 1105 3 0.25898692799368206 1105 4 0.26658290777199767 1105 5 0.24081166386587682 1105 6 0.06731365298125525 1105 7 0.1766727145837308 1105 8 0.16138686525481916 1105 9 0.21021567051716517 1105 10-11 0.38574426711235077 1105 12-13 0.23521659387441218 1105 14-15 0.3615698438547952 1105 16-17 0.3330240828752906 1105 18-19 0.40574241100279096 1105 20-21 0.4679110901055594 1105 22-23 0.5850387934013597 1105 24-25 0.4957093511257469 1105 26-27 0.5072208649511509 1105 28-29 0.2536480106468417 1105 30-31 0.27739656143061353 1105 32-33 0.4993777117710394 1105 34-35 0.3851729856410273 1105 36-37 0.21673752385505196 1105 38-39 0.2247186386166362 1105 40-41 0.4129780751229646 1105 42-43 0.5262488232214579 1105 44-45 0.6219727713001362 1105 46-47 0.611754637552707 1105 48-49 0.6583133110684614 1105 50-51 0.5751241146806905 1105 52-53 0.26373397753669536 1105 54-55 0.4278793524974347 1105 56-57 0.5652635510791484 1105 58-59 0.550956240736884 1105 60-61 0.4457987794701168 1105 62-63 0.42178125279556156 1105 64-65 0.522591146264503 1105 66-67 0.6678506210486042 1105 68-69 0.7128821881623573 1105 70-71 0.5449580334290509 1105 72-73 0.5482112993402417 1105 74-75 0.6683365394562273 1105 76-77 0.6349828651891727 1105 78-79 0.4840523191054338 1105 80-81 0.6289842736380713 1105 82-83 0.5621647907995424 1105 84-85 0.4142555975587001 1105 86-87 0.5528396240966238 1105 88-89 0.5353823442307757 1105 90-91 0.6026014518540137 1105 92-93 0.44596607290328194 1105 94-95 0.5826588158854911 1105 96-97 0.6167618091799767 1105 98-99 0.5150988373589414 1105 100-101 0.5910081312627007 1105 102-103 0.6604170966086471 1105 104-105 0.6191943933152544 1105 106-107 0.5252911834278287 1105 108-109 0.8846469502329022 1105 110-111 0.9527332889363009 1105 112-113 0.9476222408649413 1105 114-115 0.9324457793899157 1105 116-117 0.8914244825450339 1105 118-119 0.6759274441516148 1105 120-121 0.5767349316064134 1105 122-123 0.6714008215342346 1105 124-125 0.8640776629360616 1106 1 -0.022327657353699237 1106 2 0.023370055402665457 1106 3 0.02531878344940708 1106 4 0.17387769422086308 1106 5 0.18663807645966557 1106 6 0.17001742789046403 1106 7 0.18991475954783255 1106 8 0.1821142077344291 1106 9 0.20008632352980982 1106 10-11 0.14874217890179864 1106 12-13 0.11735476453221594 1106 14-15 0.17701279298353967 1106 16-17 0.16038051295075206 1106 18-19 0.33987767821963644 1106 20-21 0.17059829883506694 1106 22-23 0.2076034559962281 1106 24-25 0.13228115461597412 1106 26-27 0.1319598386327243 1106 28-29 0.14655310823471268 1106 30-31 0.43996527837999366 1106 32-33 0.1999505528272678 1106 34-35 0.24399316847932795 1106 36-37 0.1992645637839061 1106 38-39 0.32058668463574236 1106 40-41 0.3652369609165973 1106 42-43 0.32879412954319776 1106 44-45 0.47360192428880765 1106 46-47 0.3693532826135879 1106 48-49 0.2945374427608769 1106 50-51 8.585849770952336E-4 1106 52-53 0.012663715137129827 1106 54-55 0.04482895207543436 1106 56-57 0.04621244441474914 1106 58-59 -0.041758781736444206 1106 60-61 -0.0593182082752719 1106 62-63 -0.06884141758541773 1106 64-65 0.03459944679244131 1106 66-67 0.09517413674069886 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-1.5270558624564394 2216 56-57 -1.4700955939521165 2216 58-59 -1.1426840003087229 2216 60-61 -1.2797472889984718 2216 62-63 -1.2832226246136784 2216 64-65 -1.457304890700133 2216 66-67 -1.5762141155299076 2216 68-69 -1.3903417160656346 2216 70-71 -1.4382620741690566 2216 72-73 -1.155126503784306 2216 74-75 -1.2816670956149387 2216 76-77 -1.4945852075768755 2216 78-79 -1.1497647479575903 2216 80-81 -1.166391511941196 2216 82-83 -1.1942349539376806 2216 84-85 -1.577648291159658 2216 86-87 -1.7078192123574851 2216 88-89 -1.5401645320314046 2216 90-91 -1.580919154960533 2216 92-93 -1.8076387060036119 2216 94-95 -1.899915056838548 2216 96-97 -1.8407139166225654 2216 98-99 -2.0829006618090276 2216 100-101 -1.9042662762206994 2216 102-103 -1.8475699625379995 2216 104-105 -1.753735635603757 2216 106-107 -2.0644506457399814 2216 108-109 -1.861314081804192 2216 110-111 -1.8510576863936876 2216 112-113 -1.6200122971579027 2216 114-115 -1.7029202318868855 2216 116-117 -1.4501126678822942 2216 118-119 -1.5176542318585327 2216 120-121 -1.5621470012820957 2216 122-123 -1.3955188191591716 2216 124-125 -1.4583228572830151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 31.0 5 118.0 6 357.0 7 798.0 8 1310.0 9 2019.0 10 2946.0 11 5897.0 12 8444.0 13 8964.0 14 11619.0 15 15031.0 16 6770.0 17 2586.0 18 2599.0 19 3237.0 20 5193.0 21 6132.0 22 5299.0 23 2271.0 24 2794.0 25 3637.0 26 4639.0 27 5422.0 28 6759.0 29 8468.0 30 11050.0 31 15230.0 32 20250.0 33 27545.0 34 37726.0 35 58131.0 36 119567.0 37 45335.0 38 5788.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.30990787888211 20.73934431185568 12.157784947072466 15.792962862189745 2 20.16337087127539 21.345762163909693 36.99013955493292 21.500727409881996 3 28.902016905973994 22.622408821107896 28.89037842311489 19.585195849803224 4 15.623752419631549 22.727380626115405 28.38689072221011 33.26197623204293 5 14.896644503660777 34.76465819815625 28.080614700023283 22.258082598159696 6 30.67086523208497 36.26715635561073 10.132379819297881 22.929598593006414 7 26.80450366181155 40.57498415873167 12.58658815719711 20.033924022259676 8 34.71931858838135 27.92610856549233 12.917638336300428 24.436934509825896 9 32.64659100215959 14.082564259512303 18.519197030893704 34.7516477074344 10-11 29.70970606364957 24.548254443098593 19.54025837431947 26.201781118932367 12-13 33.03055317277974 25.834845617680145 20.47780282685817 20.656798382681938 14-15 34.094181189625374 19.71138717784033 24.79158494583795 21.402846686696353 16-17 30.097547728556094 20.83600946596606 26.208246942742974 22.85819586273487 18-19 28.416649065257403 20.849587695968346 26.962377526520655 23.771385712253597 20-21 33.39371694347577 17.848906629193625 27.359810163412924 21.397566263917685 22-23 29.646664281496104 20.916508972408174 27.07509838828565 22.36172835781007 24-25 24.920254839669123 22.409790981468948 29.316691739694555 23.353262439167374 26-27 22.585661389117586 22.028738431563568 31.509252593872983 23.876347585445863 28-29 23.16144299945256 19.543814577415308 33.808822832117045 23.48591959101509 30-31 24.93997560229149 21.57871709434499 29.91337951368384 23.567927789679683 32-33 23.9870209363375 19.658044131402782 31.54136618546569 24.81356874679403 34-35 22.3215324864541 20.106341248938527 32.709848311773406 24.86227795283397 36-37 22.341684303997173 21.451448129006117 30.683674656988046 25.523192910008664 38-39 23.366086323058422 17.891257775153132 32.13611421231179 26.606541689476654 40-41 22.297932229545367 17.619262120186733 33.586721784222526 26.49608386604537 42-43 22.988913267439404 17.980809434930105 34.48935078818392 24.540926509446567 44-45 22.505808465056532 18.559716193440206 33.207501217730155 25.72697412377311 46-47 21.472569820120782 17.83565169038187 31.992465159986033 28.699313329511313 48-49 20.805404566595833 19.086142015354177 32.000116384828594 28.108337033221403 50-51 20.8050812754053 19.552974494480342 30.6852911129407 28.95665311717366 52-53 21.005306286073907 19.254037906969725 29.74257400135351 29.99808180560285 54-55 19.432602407872785 20.027565962179242 27.992167732090746 32.54766389785723 56-57 20.15709796585183 20.90293074240589 26.936621995008387 32.003349296733894 58-59 19.924759363590518 20.58222588139955 26.826918517688338 32.6660962373216 60-61 20.23738194483359 22.227239222549343 24.37981973283216 33.1555590997849 62-63 18.96242925311114 23.204871782713834 24.53499950428684 33.297699459888186 64-65 18.403458784683757 22.967144993943677 24.988361517140902 33.641034704231664 66-67 19.121165227661656 24.1099793524693 24.670889568039865 32.09796585182918 68-69 19.07331813146313 24.326153395204084 24.87499407299484 31.725534400337946 70-71 18.773950489031808 23.796602425114983 25.572333170969312 31.857113914883893 72-73 19.28550491618137 23.102172947855287 25.898533982214673 31.713788153748666 74-75 19.53821086344611 22.68103229032411 25.720292772502145 32.06046407372764 76-77 19.77529106983521 22.378647263447835 25.827517684028123 32.01854398268883 78-79 20.04879541702408 22.76821314803719 26.268702395372195 30.914289039566533 80-81 19.715051144666344 23.855980240442435 25.834737853949974 30.594230760941247 82-83 18.627715107181807 23.670626624538233 26.252968890766372 31.44868937751359 84-85 19.223971826250384 22.548806193397102 26.390583174202227 31.83663880615029 86-87 19.959782575897993 22.53609007323623 25.808874558707522 31.69525279215825 88-89 19.90611623826992 22.876623460595113 25.50250227381471 31.71475802732026 90-91 19.79307208531439 22.310001767325176 25.40001896641651 32.49690718094392 92-93 19.743608533163208 22.32390328851799 25.38838048355741 32.54410769476139 94-95 19.614723111871683 22.26075374263435 25.275120803141526 32.84940234235244 96-97 19.781218074994936 22.414424821866554 25.026186586432974 32.778170516705536 98-99 20.32779571445198 23.100772019362985 24.68295910581967 31.88847316036536 100-101 20.518429753134846 23.97495139855769 24.241666630745424 31.26495221756204 102-103 20.105802430287643 25.214018768131247 23.889925815448144 30.790252986132966 104-105 19.830681627146117 26.819913875226842 23.70575760057589 29.643646897051152 106-107 19.88986534907404 28.864653997230473 23.00607249273941 28.23940816095608 108-109 19.595972222820908 29.826953002081996 22.617882744440468 27.959192030656627 110-111 19.31364466644036 30.837711286768073 22.466741024521927 27.38190302226964 112-113 19.518187843160497 32.04799801714523 22.288257512487135 26.14555662720714 114-115 19.125060617490167 32.34700145481976 22.476426531601916 26.051511396088152 116-117 18.4319401321894 32.5761845694253 23.027208428394534 25.964666869990765 118-119 18.570892820194494 33.30520639354438 23.113770777294988 25.010130008966136 120-121 18.902203782531387 33.45600517269249 22.978608761247912 24.663182283528208 122-123 18.74538626089112 33.22204680284718 23.048381621557546 24.984185314704156 124-125 17.55091349475747 34.90100797563008 23.522772962409224 24.025305567203226 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 1.5 4 2.5 5 1.5 6 3.0 7 5.0 8 7.0 9 13.5 10 18.5 11 22.5 12 30.0 13 32.0 14 34.0 15 48.0 16 58.0 17 61.0 18 73.5 19 107.0 20 153.0 21 232.0 22 324.0 23 464.0 24 678.0 25 941.5 26 1330.0 27 1769.0 28 2342.0 29 2976.5 30 3525.5 31 4184.5 32 4674.5 33 4949.0 34 5325.0 35 5426.0 36 5237.0 37 4913.0 38 4504.0 39 4116.0 40 3881.0 41 3779.0 42 3759.5 43 3900.5 44 4127.0 45 4560.5 46 5319.5 47 6545.5 48 7844.5 49 10471.5 50 16330.5 51 26161.0 52 37740.5 53 45186.0 54 44587.5 55 39185.0 56 33132.0 57 25664.5 58 18526.0 59 13529.0 60 9900.0 61 7091.0 62 5359.5 63 4625.5 64 3953.5 65 3445.0 66 3114.5 67 2978.5 68 2818.0 69 2242.0 70 1418.5 71 970.0 72 908.0 73 839.5 74 586.5 75 329.5 76 216.0 77 129.5 78 80.0 79 54.5 80 46.0 81 23.0 82 11.5 83 9.5 84 4.5 85 1.0 86 2.0 87 2.0 88 0.5 89 1.0 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19225049463552152 2 6.46582381061171E-4 3 0.0 4 0.12522145446551344 5 0.030604899370228762 6 0.0021552746035372367 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 1.0776373017686184E-4 108-109 0.0 110-111 0.00118540103194548 112-113 1.0776373017686184E-4 114-115 6.46582381061171E-4 116-117 0.004849367857958782 118-119 0.0021552746035372367 120-121 6.46582381061171E-4 122-123 0.0022630383337140987 124-125 0.027479751195099766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 463978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.05192540319591 #Duplication Level Percentage of deduplicated Percentage of total 1 80.8660662710216 42.90100515460134 2 8.976408086882252 9.524314644278434 3 3.270646290603159 5.2054224908795454 4 1.7567320331819645 3.72792067111097 5 1.102324093121609 2.924020777921659 6 0.7551484745778216 2.403724832498386 7 0.5714989496796216 2.122338375248566 8 0.4273810437922912 1.813870980320691 9 0.3325469918375725 1.5878032383621687 >10 1.8291838867916532 16.800212924068557 >50 0.0791755663421098 2.8233259306326772 >100 0.02679788398904885 2.750949527448252 >500 0.002842199817020333 0.9553254368673554 >1k 0.002842199817020333 2.869853617899386 >5k 4.0602854528861896E-4 1.5899113978619956 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 7381 1.5908081848708344 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 4165 0.8976718723732591 RNA PCR Primer, Index 46 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 2365 0.5097224437365565 RNA PCR Primer, Index 46 (95% over 23bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1669 0.35971533133036476 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1445 0.3114371802111307 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1383 0.29807447766919987 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 1167 0.2515205462327955 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 1129 0.24333050273935403 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 978 0.21078585622594176 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 714 0.15388660669255871 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 655 0.14117048653168898 No Hit GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT 557 0.12004879541702407 No Hit GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT 523 0.11272086176499747 No Hit ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA 504 0.10862584001827673 No Hit GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 504 0.10862584001827673 No Hit TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT 488 0.10517740065261715 No Hit GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT 484 0.10431529081120225 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 484 0.10431529081120225 TruSeq Adapter, Index 4 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.1552746035372367E-4 0.0 4 0.0 0.0 0.0 2.1552746035372367E-4 0.0 5 2.1552746035372367E-4 0.0 0.0 2.1552746035372367E-4 0.0 6 2.1552746035372367E-4 0.0 0.0 2.1552746035372367E-4 0.0 7 4.3105492070744735E-4 0.0 0.0 2.1552746035372367E-4 0.0 8 6.46582381061171E-4 0.0 0.0 2.1552746035372367E-4 0.0 9 6.46582381061171E-4 0.0 0.0 2.1552746035372367E-4 0.0 10-11 0.00237080206389096 0.0 0.0 2.1552746035372367E-4 0.0 12-13 0.004095021746720749 0.0 0.0 2.1552746035372367E-4 0.0 14-15 0.004526076667428197 0.0 0.0 4.3105492070744735E-4 0.0 16-17 0.0054959502390199534 0.0 0.0 6.46582381061171E-4 0.0 18-19 0.0062502963502579865 0.0 0.0 8.621098414148947E-4 0.0 20-21 0.0065735875407885724 0.0 0.0 0.0021552746035372367 0.0 22-23 0.007220169921849743 0.0 0.0 0.015517977145468103 0.0 24-25 0.008405570953795223 0.0 0.0 0.039441525244731435 0.0 26-27 0.009698735715917565 0.0 0.0 0.11002676851057594 0.0 28-29 0.012500592700515973 0.0 0.0 0.36887524839539804 0.0 30-31 0.01465586730405321 0.0 0.0 0.9145907780110263 0.0 32-33 0.014979158494583795 0.0 0.0 1.6993262611589341 0.0 34-35 0.01584126833599869 0.0 0.0 2.847656569923574 0.0 36-37 0.020259581273250026 0.0 0.0 4.37660837367289 0.0 38-39 0.022199328416433538 0.0 0.0 6.455262965054377 0.0 40-41 0.02424683928979391 0.0 0.0 8.764639702744526 0.0 42-43 0.029527262068460142 0.0 0.0 10.62367181202557 0.0 44-45 0.030820426830582483 0.0 0.0 12.23764919888443 0.0 46-47 0.031035954290936207 0.0 0.0 14.183862165878555 0.0 48-49 0.031035954290936207 0.0 0.0 16.335580566319955 0.0 50-51 0.03146700921164366 0.0 0.0 18.34440426054684 0.0 52-53 0.03146700921164366 0.0 0.0 20.19697054601727 0.0 54-55 0.031682536671997386 0.0 0.0 21.977873950920085 0.0 56-57 0.032005827862527966 0.0 0.0 23.817831879959826 0.0 58-59 0.03232911905305855 0.0 0.0 25.677726099082285 0.0 60-61 0.03232911905305855 0.0 0.0 27.579324881783187 0.0 62-63 0.03286793770394286 0.0 0.0 29.326713766601003 0.0 64-65 0.03351452008500403 0.0 0.0 31.00675032005828 0.0 66-67 0.03373004754535776 0.0 0.0 32.851880908146505 0.0 68-69 0.03437662992641892 0.0 0.0 34.803589825379646 0.0 70-71 0.034915448577303235 0.0 0.0 36.915866700576316 0.0 72-73 0.034915448577303235 0.0 0.0 39.00626753854709 0.0 74-75 0.034915448577303235 0.0 0.0 40.82467272155145 0.0 76-77 0.034915448577303235 0.0 0.0 42.75191927203445 0.0 78-79 0.034915448577303235 0.0 0.0 44.64478919259103 0.0 80-81 0.034915448577303235 0.0 0.0 46.45856053519779 0.0 82-83 0.034915448577303235 0.0 0.0 48.38084995409265 0.0 84-85 0.034915448577303235 0.0 0.0 50.44915922737716 0.0 86-87 0.034915448577303235 0.0 0.0 52.132320929009566 0.0 88-89 0.035023212307480095 0.0 0.0 53.62527964687981 0.0 90-91 0.035130976037656955 0.0 0.0 55.51567100164232 0.0 92-93 0.035130976037656955 0.0 0.0 57.145058601916475 0.0 94-95 0.035130976037656955 0.0 0.0 58.57928608684033 0.0 96-97 0.035130976037656955 0.0 0.0 59.945299130562226 0.0 98-99 0.035130976037656955 0.0 0.0 61.17229696235597 0.0 100-101 0.035130976037656955 0.0 0.0 62.356189302078974 0.0 102-103 0.035130976037656955 0.0 0.0 63.39848010034959 0.0 104-105 0.035130976037656955 0.0 0.0 64.40553215885237 0.0 106-107 0.035130976037656955 0.0 0.0 65.33003720003965 0.0 108-109 0.035130976037656955 0.0 0.0 66.20874265590179 0.0 110-111 0.035130976037656955 0.0 0.0 67.10749216557682 0.0 112-113 0.035130976037656955 0.0 0.0 67.9882451323123 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGATT 30 0.0042074574 59.59797 2 TATAACG 30 0.0042074574 59.59797 2 TAGGGTC 30 0.004230892 59.514442 5 CTCGGAG 45 3.1433144E-4 52.975975 3 CTATACA 70 8.9059176E-7 51.183434 1 CTACGGG 70 9.026244E-7 51.083973 3 TCTACGG 75 1.4523448E-6 47.678375 2 GTTGTAG 50 5.331413E-4 47.59616 8 GTTCAAG 85 3.391593E-6 42.151066 1 GTCTATG 60 0.0012848211 39.809338 1 AGTTGTA 75 8.184984E-5 39.66347 7 GAATGGT 30 0.0013089004 39.663467 50-51 GTCTACG 95 7.2809944E-6 37.714115 1 CTATGGG 65 0.0019221184 36.67568 3 TCGGAGA 65 0.0019221184 36.67568 4 TGCGAGG 65 0.0019221184 36.67568 2 CGTAATG 65 4.2042302E-8 36.612434 26-27 AGTAGCG 65 0.0019384762 36.612434 7 CTTTACA 140 7.7980076E-8 34.122288 1 TCTATGG 70 0.002764886 34.055984 2 >>END_MODULE