FastQCFastQC Report
Thu 26 May 2016
SRR935983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673478
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT75990.45408424849325774No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC74510.445240391567741No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT65130.38918946051277636No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT56810.339472643201763No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG55920.3341543778884455No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT42660.25491820029901796No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA38670.23107564007414497No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC22920.13696027076543582No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC21910.13092493597167099No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC19710.11777866216347033No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA18780.1122213736900037No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG18420.11007016524866176No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG18050.10785920101728257No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG16750.10009094831243673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT17250.064.59931
AGTAGTA12850.064.363182
GAGTACG7600.061.158421
GAGTCAG41050.060.969331
GAGTAAG13000.058.673491
GGGGTAA53300.057.3647239
AGGGTAA45000.056.180486
GTGGGGT46100.056.0012637
TGGGGTA46350.055.6992038
GATAGCG13150.053.8307239
AGTACGG5200.053.7799382
GATTGTG41050.053.0367559
GAGTCCG8700.050.685971
GTTAACG1300.050.3426673
GTAGTAG26900.049.8431551
GAGTCGT7800.049.6585461
TCGTAGG8650.049.527022
GAGTTAG12300.049.4163131
GTAAGGG9500.048.8492473
GGTATCA36700.048.7113841
Produced by FastQC (version 0.11.5)