##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935983_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1673478 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.9164745518017 31.0 31.0 34.0 28.0 34.0 2 31.038066828485345 33.0 31.0 34.0 27.0 34.0 3 31.123545095902067 34.0 31.0 34.0 27.0 34.0 4 34.39573690242716 37.0 35.0 37.0 30.0 37.0 5 34.47247230020353 37.0 35.0 37.0 31.0 37.0 6 34.566467560374264 37.0 35.0 37.0 31.0 37.0 7 34.458731456284454 37.0 35.0 37.0 31.0 37.0 8 34.437822905350416 37.0 35.0 37.0 31.0 37.0 9 36.00356323775992 39.0 37.0 39.0 31.0 39.0 10-11 35.8812954218699 39.0 37.0 39.0 30.0 39.0 12-13 35.75272576036255 39.0 37.0 39.0 30.0 39.0 14-15 37.16773211240303 40.0 38.0 41.0 30.0 41.0 16-17 37.30406016690988 40.0 38.0 41.0 30.5 41.0 18-19 37.38846940324282 40.0 38.0 41.0 30.5 41.0 20-21 37.49080955949226 40.0 38.0 41.0 31.0 41.0 22-23 37.417623058086214 40.0 38.0 41.0 30.0 41.0 24-25 37.395414519939905 40.0 37.5 41.0 30.0 41.0 26-27 37.36135760374502 40.0 37.0 41.0 30.0 41.0 28-29 37.269511161784024 40.0 37.0 41.0 30.0 41.0 30-31 37.19541547603255 40.0 37.0 41.0 30.0 41.0 32-33 37.11780794250059 40.0 37.0 41.0 30.0 41.0 34-35 36.95786051564467 40.0 36.0 41.0 30.0 41.0 36-37 36.9484663078929 40.0 36.0 41.0 30.0 41.0 38-39 37.008476657595736 40.0 36.0 41.0 30.0 41.0 40-41 36.9543131131691 40.0 36.0 41.0 30.0 41.0 42-43 36.811278367567425 40.0 35.5 41.0 30.0 41.0 44-45 36.66446257435114 40.0 35.0 41.0 30.0 41.0 46-47 36.51933398586656 40.0 35.0 41.0 30.0 41.0 48-49 36.341338218966726 40.0 35.0 41.0 30.0 41.0 50-51 36.129040835911795 39.0 35.0 41.0 30.0 41.0 52-53 35.923842141934344 39.0 35.0 41.0 29.5 41.0 54-55 35.69200969477937 39.0 35.0 41.0 29.0 41.0 56-57 35.44268224619624 38.0 35.0 41.0 29.0 41.0 58-59 35.12202759761407 37.5 34.5 40.0 28.5 41.0 60-61 34.84789372791276 37.0 34.0 40.0 28.0 41.0 62-63 34.49830801480509 36.5 34.0 40.0 28.0 41.0 64-65 34.15649115196017 36.0 34.0 39.0 27.0 41.0 66-67 33.892652308545436 35.0 34.0 39.0 27.5 41.0 68-69 33.679544636977596 35.0 34.0 38.5 28.0 40.5 70-71 33.40321354687663 35.0 34.0 37.0 28.0 40.0 72-73 33.10537455526753 35.0 34.0 37.0 27.0 39.0 74-75 32.78422214095435 35.0 33.5 36.0 27.0 39.0 76-77 32.51960916127968 35.0 33.0 36.0 26.5 38.5 78-79 32.261275618801086 35.0 33.0 35.5 26.0 37.0 80-81 32.018333375162385 35.0 33.0 35.0 26.0 37.0 82-83 31.827839684776258 35.0 33.0 35.0 25.0 36.5 84-85 31.617022452640548 35.0 33.0 35.0 25.0 36.0 86-87 31.43624415737763 35.0 33.0 35.0 24.0 36.0 88-89 31.285502707534846 35.0 33.0 35.0 24.0 36.0 90-91 31.156632175624658 35.0 33.0 35.0 23.5 35.5 92-93 31.005939426750754 35.0 33.0 35.0 21.5 35.0 94-95 30.84507415095986 35.0 32.0 35.0 20.0 35.0 96-97 30.715609048938795 35.0 32.0 35.0 19.0 35.0 98-99 30.55868885040616 35.0 32.0 35.0 18.0 35.0 100-101 30.44142349047911 35.0 32.0 35.0 16.0 35.0 102-103 29.743769562551762 34.0 30.5 35.0 15.0 35.0 104-105 30.226654010390334 35.0 31.5 35.0 13.0 35.0 106-107 30.24435666318888 35.0 32.0 35.0 8.5 35.0 108-109 30.145919755144675 35.0 32.0 35.0 6.0 35.0 110-111 30.02185299119558 35.0 31.0 35.0 2.0 35.0 112-113 29.900345866512737 35.0 31.0 35.0 2.0 35.0 114-115 29.710640056218246 35.0 31.0 35.0 2.0 35.0 116-117 29.5125884535082 35.0 31.0 35.0 2.0 35.0 118-119 29.279184727854204 35.0 31.0 35.0 2.0 35.0 120-121 29.05472943175829 35.0 30.5 35.0 2.0 35.0 122-123 28.694523322087292 35.0 30.0 35.0 2.0 35.0 124-125 26.036690055082886 32.0 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24285440840815653 1101 2 0.39929709032991667 1101 3 0.39756222449818424 1101 4 0.44324023469708607 1101 5 0.36537794433070303 1101 6 0.4430251686542235 1101 7 0.35445581506833435 1101 8 0.18299460299719783 1101 9 0.33162039336158244 1101 10-11 0.44137024027448035 1101 12-13 0.3501722508629044 1101 14-15 0.40112382865333984 1101 16-17 0.3018928412149151 1101 18-19 0.30909165493655166 1101 20-21 0.23509458566629604 1101 22-23 0.325596876058583 1101 24-25 0.42691114133815944 1101 26-27 0.434479958856123 1101 28-29 0.40836640119424317 1101 30-31 0.2994147180472595 1101 32-33 0.2991548815228029 1101 34-35 0.11387504844984875 1101 36-37 0.1618703487424753 1101 38-39 0.2659055741412004 1101 40-41 0.17779648210074583 1101 42-43 0.037823463221187126 1101 44-45 0.1358883717149908 1101 46-47 0.07592173968949822 1101 48-49 0.022934882889352082 1101 50-51 0.13450920876071848 1101 52-53 0.056627941941044924 1101 54-55 -0.019347894570515223 1101 56-57 -0.10747633794844802 1101 58-59 -0.16822167359995177 1101 60-61 -0.01750201547625352 1101 62-63 0.03493142223899781 1101 64-65 -0.04157418875184504 1101 66-67 -0.008331718031264757 1101 68-69 0.13316496792746335 1101 70-71 0.07058816617066554 1101 72-73 0.08153535612015617 1101 74-75 0.21488156490997312 1101 76-77 0.2999103177363054 1101 78-79 0.3530711676851297 1101 80-81 0.3764693870717011 1101 82-83 0.27146611992068514 1101 84-85 0.12837680860397072 1101 86-87 0.05563803539901713 1101 88-89 0.05682372263809299 1101 90-91 0.037742072000138904 1101 92-93 -0.13107822789848456 1101 94-95 -0.11898390722948093 1101 96-97 -0.0016015794009085482 1101 98-99 -0.07054777558216685 1101 100-101 -0.10812127458683563 1101 102-103 -0.152372740151403 1101 104-105 -0.11915852777161362 1101 106-107 -0.006740879079199402 1101 108-109 0.16626665128562834 1101 110-111 0.12221918807463794 1101 112-113 0.11962377353942699 1101 114-115 0.15680621289568464 1101 116-117 0.18450393142879307 1101 118-119 0.13239293760471682 1101 120-121 -0.1515310787961397 1101 122-123 -0.05664609141497223 1101 124-125 0.13091336601418746 1102 1 0.22609275261238082 1102 2 0.20439408884444532 1102 3 0.24100952923994612 1102 4 0.3213408874169801 1102 5 0.3292989820908261 1102 6 0.3416940382602718 1102 7 0.2399111293680818 1102 8 0.24391629293309336 1102 9 0.24980342699721803 1102 10-11 0.2655527080174309 1102 12-13 0.2812165171962633 1102 14-15 0.3825895800974166 1102 16-17 0.3560080781086228 1102 18-19 0.32610312359130944 1102 20-21 0.2554390300848297 1102 22-23 0.2758034696411471 1102 24-25 0.3366529100481799 1102 26-27 0.285971412555142 1102 28-29 0.1312937220732735 1102 30-31 0.199325753187793 1102 32-33 0.33154601908370296 1102 34-35 0.3579926406386633 1102 36-37 0.2758786801130597 1102 38-39 0.21003882943109886 1102 40-41 0.21675145010216568 1102 42-43 0.12832412278154237 1102 44-45 0.14892440539804142 1102 46-47 0.20398673261972533 1102 48-49 0.19963953743440754 1102 50-51 0.16050529259678825 1102 52-53 0.27246625209843245 1102 54-55 0.2890915047748166 1102 56-57 0.2427237844025214 1102 58-59 0.1292103162823821 1102 60-61 0.0685741095269492 1102 62-63 0.14619210365773938 1102 64-65 0.12385487916538551 1102 66-67 0.13230233051891105 1102 68-69 0.3289030109182036 1102 70-71 0.4094030018868935 1102 72-73 0.32568530446690147 1102 74-75 0.34147721904071915 1102 76-77 0.3826524953193342 1102 78-79 0.3846563162669341 1102 80-81 0.3975512215278769 1102 82-83 0.32367535563131256 1102 84-85 0.30553814165435966 1102 86-87 0.31781084954135963 1102 88-89 0.2866722491794711 1102 90-91 0.3998265237099794 1102 92-93 0.47412184392635837 1102 94-95 0.4011666917383856 1102 96-97 0.29066634824256 1102 98-99 0.28750643973533485 1102 100-101 0.43126685783516194 1102 102-103 0.37060839105874166 1102 104-105 0.24546233578994858 1102 106-107 0.16733252995005543 1102 108-109 0.3784423026646415 1102 110-111 0.352340458565898 1102 112-113 0.38943050330934526 1102 114-115 0.4207049802912515 1102 116-117 0.27821863601027275 1102 118-119 0.23727974740802793 1102 120-121 0.08977337379985428 1102 122-123 0.16772812300083473 1102 124-125 0.04946553191402003 1103 1 0.14316152207141997 1103 2 0.2008192505977071 1103 3 0.22644489482663488 1103 4 0.13401191412451396 1103 5 0.12430180973239402 1103 6 0.18272813414468203 1103 7 0.11654386433597352 1103 8 0.15753260325611507 1103 9 0.06865041814335626 1103 10-11 0.1747101620454501 1103 12-13 0.2571819439572991 1103 14-15 0.21195851435018653 1103 16-17 0.23573943695762267 1103 18-19 0.18629069048175495 1103 20-21 0.24729359809417417 1103 22-23 0.2869525106753912 1103 24-25 0.332501141708029 1103 26-27 0.35355660665904765 1103 28-29 0.19543329810947085 1103 30-31 0.26505283712921823 1103 32-33 0.27843424514907866 1103 34-35 0.24625063100643985 1103 36-37 0.2604247068205936 1103 38-39 0.17426156245308277 1103 40-41 0.22968562990368468 1103 42-43 0.19533884780100408 1103 44-45 0.11823309264264026 1103 46-47 -0.008468344365098801 1103 48-49 0.09486819634178545 1103 50-51 0.07054790485111795 1103 52-53 0.014649191386226335 1103 54-55 0.09823520065437208 1103 56-57 0.0537609359641138 1103 58-59 -0.039881509097071444 1103 60-61 0.06903740187468088 1103 62-63 -0.24824945233564222 1103 64-65 -0.2581632967929792 1103 66-67 -0.06623079241357033 1103 68-69 0.02559398618090114 1103 70-71 -0.01365512286986359 1103 72-73 0.0011780894202004788 1103 74-75 -0.03696839902700333 1103 76-77 0.025179692366428696 1103 78-79 0.026530571350605214 1103 80-81 -0.035560233156886056 1103 82-83 0.06363038700900958 1103 84-85 0.11299627431635528 1103 86-87 0.12486459905488445 1103 88-89 0.08902124399850209 1103 90-91 0.024343848335394824 1103 92-93 0.1878079331460505 1103 94-95 0.09695502604467165 1103 96-97 0.015270022352307677 1103 98-99 0.2267554728643475 1103 100-101 0.23149968219301442 1103 102-103 0.23580818065788733 1103 104-105 0.19669464022661387 1103 106-107 0.17481247801874744 1103 108-109 0.12266882107775601 1103 110-111 0.10627141242365212 1103 112-113 0.19951522613966333 1103 114-115 0.06847433595383379 1103 116-117 0.1780042065249603 1103 118-119 0.1285025807656872 1103 120-121 -0.01785601360528588 1103 122-123 -0.07522519658955318 1103 124-125 -0.18807422229399506 1104 1 0.21454697398907285 1104 2 0.2670228074222969 1104 3 0.3174783656388165 1104 4 0.3131243823986267 1104 5 0.28181423570531905 1104 6 0.22281511595517145 1104 7 0.15532876281313435 1104 8 0.1668807243785011 1104 9 0.31092604561091974 1104 10-11 0.40950156432970886 1104 12-13 0.36983638734421476 1104 14-15 0.40118691430788544 1104 16-17 0.4243685701358473 1104 18-19 0.3887040611811301 1104 20-21 0.3907739280434086 1104 22-23 0.41423391049208647 1104 24-25 0.3240629366882857 1104 26-27 0.462098802090523 1104 28-29 0.4069326107152236 1104 30-31 0.48030748144675783 1104 32-33 0.47728285978529783 1104 34-35 0.5160660920893534 1104 36-37 0.46859280115223356 1104 38-39 0.37936061772663265 1104 40-41 0.505753734810618 1104 42-43 0.43611080035317684 1104 44-45 0.3296134331671752 1104 46-47 0.3657946896873554 1104 48-49 0.4424175917873612 1104 50-51 0.48176278605529177 1104 52-53 0.46026573114934877 1104 54-55 0.4634012768670033 1104 56-57 0.4423931536037031 1104 58-59 0.4181907466135897 1104 60-61 0.5232144235187945 1104 62-63 0.5331745994128099 1104 64-65 0.45901583109760224 1104 66-67 0.4351021355334126 1104 68-69 0.4233209584736244 1104 70-71 0.4673963700094248 1104 72-73 0.3658043848939698 1104 74-75 0.4864624914129365 1104 76-77 0.479746678054525 1104 78-79 0.42146390292138847 1104 80-81 0.43707949294294224 1104 82-83 0.45943067254200187 1104 84-85 0.4199772916598441 1104 86-87 0.41692563613892375 1104 88-89 0.3981140157836087 1104 90-91 0.4080971090516954 1104 92-93 0.3803532306643831 1104 94-95 0.4493415178664435 1104 96-97 0.34352060399359274 1104 98-99 0.3706786433381204 1104 100-101 0.36927265702381007 1104 102-103 0.4552874354520213 1104 104-105 0.46566407762086115 1104 106-107 0.4653169637068437 1104 108-109 0.5209091358690721 1104 110-111 0.44211527698936237 1104 112-113 0.46609751075613204 1104 114-115 0.39864169001474536 1104 116-117 0.31371940498802076 1104 118-119 0.49532104699524027 1104 120-121 0.5745819887212811 1104 122-123 0.4092434388447401 1104 124-125 0.5961379622096707 1105 1 0.15547420481995644 1105 2 0.16913522281992854 1105 3 0.09507153956347736 1105 4 0.15683263780872636 1105 5 0.06543393692537336 1105 6 0.13975938414468203 1105 7 0.14313377661667914 1105 8 0.15476853161868576 1105 9 0.12982549124276943 1105 10-11 0.22302430678229257 1105 12-13 0.21194025901578328 1105 14-15 0.28148291128001546 1105 16-17 0.2928369333026595 1105 18-19 0.2626795793706407 1105 20-21 0.23447835686609864 1105 22-23 0.23451510204967008 1105 24-25 0.3004744604214764 1105 26-27 0.3818597864836093 1105 28-29 0.30158735512700474 1105 30-31 0.2890956916613874 1105 32-33 0.2939907144473253 1105 34-35 0.35089701258538497 1105 36-37 0.32847569366269624 1105 38-39 0.34787267356419704 1105 40-41 0.3019169895528009 1105 42-43 0.29615893186532816 1105 44-45 0.22705984702860604 1105 46-47 0.23142337712613426 1105 48-49 0.185408675142952 1105 50-51 0.15037475061135552 1105 52-53 0.15068263436869245 1105 54-55 0.2592709279935548 1105 56-57 0.2944750258763875 1105 58-59 0.266484993095915 1105 60-61 0.21972499324895267 1105 62-63 0.16503636637780517 1105 64-65 0.24846133770994783 1105 66-67 0.30927176606596873 1105 68-69 0.3053020453914286 1105 70-71 0.28437347727633266 1105 72-73 0.2674541310868648 1105 74-75 0.26600583342914064 1105 76-77 0.3071721083021046 1105 78-79 0.3408237913798473 1105 80-81 0.2784817076325936 1105 82-83 0.3264456282370851 1105 84-85 0.39959166905319776 1105 86-87 0.4064800961309061 1105 88-89 0.4894803997002555 1105 90-91 0.44969107055761626 1105 92-93 0.4093448483507274 1105 94-95 0.3780222775066626 1105 96-97 0.3399684872645885 1105 98-99 0.4437236746187345 1105 100-101 0.3474878949123088 1105 102-103 0.3764103370906362 1105 104-105 0.46451262268275073 1105 106-107 0.38457353430529295 1105 108-109 0.3711497238555346 1105 110-111 0.34552351403183934 1105 112-113 0.3363139286250423 1105 114-115 0.42551929209418304 1105 116-117 0.3785821487764238 1105 118-119 0.26939028178907876 1105 120-121 0.4383186026227861 1105 122-123 0.5093987982934891 1105 124-125 0.6248940708346602 1106 1 0.05676425294273457 1106 2 0.09660896664200536 1106 3 0.05303184910999548 1106 4 -0.435315979203331 1106 5 0.01609589608604267 1106 6 0.1191019500425412 1106 7 0.13920483358364066 1106 8 0.11129613176608899 1106 9 0.21779723536832307 1106 10-11 0.20572205454956816 1106 12-13 0.17318326820679886 1106 14-15 0.20851024588506561 1106 16-17 0.19438845935832205 1106 18-19 0.12271741915647283 1106 20-21 0.2087244739865426 1106 22-23 0.2516886475278568 1106 24-25 0.2647942384497455 1106 26-27 0.23422399408354266 1106 28-29 0.18289940533077242 1106 30-31 0.09983376393225996 1106 32-33 0.1921470917992778 1106 34-35 0.19676288388946972 1106 36-37 0.17964191233954097 1106 38-39 0.23705615791345735 1106 40-41 0.16150537180373448 1106 42-43 0.07786855045979735 1106 44-45 -0.03341993110804253 1106 46-47 0.07162479642293107 1106 48-49 -0.0385939152498338 1106 50-51 -0.17556765379340078 1106 52-53 -0.1416093890640724 1106 54-55 -0.12012474637565163 1106 56-57 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-1.4211798561325963 2216 82-83 -1.5413322901164968 2216 84-85 -1.5129367975459935 2216 86-87 -1.479975074246962 2216 88-89 -1.3512120766830087 2216 90-91 -1.40813935285669 2216 92-93 -1.3402123378840898 2216 94-95 -1.1503599762045411 2216 96-97 -1.2152198140174626 2216 98-99 -1.1370845659345754 2216 100-101 -1.2176742592712237 2216 102-103 -1.1783825947582187 2216 104-105 -1.5063143847396496 2216 106-107 -1.2174686806069843 2216 108-109 -1.488720805834074 2216 110-111 -1.6466692575606032 2216 112-113 -1.7099970109835958 2216 114-115 -1.7238091070286252 2216 116-117 -1.7249519267971252 2216 118-119 -1.774079269492976 2216 120-121 -1.6105490593205367 2216 122-123 -1.5185037405054338 2216 124-125 -1.4420709679642627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24466.0 3 4860.0 4 3440.0 5 2770.0 6 3265.0 7 3624.0 8 4035.0 9 4591.0 10 5811.0 11 6443.0 12 6693.0 13 6442.0 14 6660.0 15 6641.0 16 6402.0 17 6155.0 18 5737.0 19 5494.0 20 5282.0 21 5556.0 22 6388.0 23 8175.0 24 11814.0 25 18266.0 26 23900.0 27 20547.0 28 21916.0 29 28582.0 30 31170.0 31 37543.0 32 50541.0 33 68883.0 34 109811.0 35 159311.0 36 312055.0 37 506299.0 38 133442.0 39 468.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.611074137204575 14.242887443980543 9.681730474585361 19.464307944229517 2 14.435891781015956 25.253188123332876 40.12974229050009 20.181177805151084 3 29.172680164391846 20.92302782673238 23.440061339170835 26.464230669704943 4 13.970738529395943 15.755234014068934 40.6923302195208 29.581697237014325 5 16.434885288535547 34.63914531712378 30.105425070633963 18.820544323706716 6 33.580961327247806 36.25700487248712 15.110745405676083 15.051288394588994 7 36.91312344709641 28.325678616629556 17.22197722348307 17.539220712790964 8 34.80490285477993 29.793292277023724 16.33135620870118 19.070448659495156 9 40.341193609954836 14.151246685047546 14.867419828644296 30.640139876353317 10-11 33.343252794479525 25.15070410247401 22.04086339945909 19.465179703587378 12-13 28.647822080720513 25.26319437721918 24.36981543826689 21.719168103793418 14-15 25.254649299243848 25.68079771589468 22.923336906729578 26.14121607813189 16-17 25.1101299210387 29.07552414791231 22.77824984851907 23.036096082529916 18-19 25.044846720422974 25.712647551984553 24.81242657507299 24.430079152519482 20-21 25.851848664876382 26.015340506418372 23.60264072787333 24.53017010083192 22-23 25.925378164517248 24.63217323442555 23.565203725414975 25.877244875642226 24-25 23.956843173319278 24.96689529231935 27.78877284314464 23.287488691216733 26-27 22.669375620905758 24.711249878619928 27.099710928688275 25.519663571786044 28-29 23.641708379348756 25.167030009226988 24.557863497937074 26.63339811348718 30-31 24.103693027335886 26.355231440150394 24.097090012536764 25.44398551997696 32-33 26.580002844375606 25.088707470310332 23.256565069872444 25.074724615441614 34-35 26.06102380790187 23.2277926569695 25.56000138633433 25.15118214879431 36-37 24.83719535004344 25.65513260407367 26.098401054570182 23.409270991312702 38-39 23.922065303517584 27.045649838241076 24.903524276984818 24.12876058125652 40-41 23.278077154285864 26.686816319067237 25.686205614893055 24.348900911753844 42-43 23.378018713123208 24.13757456028702 24.732323938528022 27.75208278806175 44-45 23.65525570100115 25.037795537198576 24.401157350141442 26.905791411658832 46-47 24.418904819782515 24.242894140227715 24.85945438183233 26.478746658157444 48-49 22.021054355061732 24.610571516327074 25.64593021240793 27.72244391620326 50-51 23.617848576437815 25.433558134615453 25.3877851994469 25.56080808949983 52-53 24.231331394855506 24.262015993039647 26.546449968269677 24.960202643835174 54-55 22.956710515465396 25.11864512111901 26.303154269132907 25.621490094282684 56-57 22.89370668065753 26.04147183731079 25.31926120110692 25.745560280924764 58-59 23.572673199169632 24.591091128774924 27.09671116082793 24.739524511227515 60-61 24.8069887981796 26.487441125607862 26.044620843536638 22.6609492326759 62-63 25.226623833716367 25.610973075236128 25.16582231735344 23.996580773694067 64-65 26.46683235202901 23.528237721645006 26.01256204984544 23.992367876480547 66-67 26.283868855084354 25.39712227894378 24.416439544732317 23.90256932123955 68-69 23.82419897925188 26.84195256249251 25.23959733007526 24.094251128180353 70-71 24.027577641681912 26.66404489211179 24.75138464958967 24.55699281661663 72-73 23.467560933051924 26.80187824623717 24.638058747182512 25.092502073528394 74-75 22.67663357709774 26.86613634910362 25.301269260166958 25.155960813631683 76-77 23.14771104352035 26.6078567288329 24.549230042964616 25.695202184682124 78-79 23.39708645647746 27.100370758676405 23.579522791246042 25.92301999360009 80-81 22.871428029353496 24.812402541196747 25.239331482282108 27.076837947167654 82-83 23.991860066281124 23.243598063434355 26.23488327901532 26.5296585912692 84-85 24.49586471602869 23.73748029180677 27.535797490021885 24.23085750214265 86-87 25.037064481142078 24.529665030311904 25.433643971830396 24.99962651671562 88-89 23.147804487667965 25.431174368546277 26.268361181785636 25.15265996200012 90-91 22.278996879252933 26.409826245049604 25.990908153829377 25.320268721868082 92-93 23.59943092426398 24.31988965118263 26.347632840367442 25.73304658418595 94-95 22.969671814446833 24.344245957930262 26.70274624972845 25.983335977894455 96-97 22.81043064414056 23.677210813821393 26.95062140808676 26.561737133951286 98-99 22.271389251084894 24.831872664234144 27.253031789721017 25.643706294959944 100-101 22.04868035979187 26.66478434747015 25.737010130163867 25.549525162574117 102-103 22.738930538674545 25.87491439983077 25.84357248795622 25.542582573538464 104-105 22.86727149684264 24.550986048471067 25.745687485154374 26.836054969531915 106-107 22.226229218231428 25.533833454240373 24.918850861268435 27.321086466259764 108-109 23.209447629428055 25.70667197298082 25.63087175331854 25.453008644272586 110-111 23.589404820380068 26.22681624736029 24.9772330439958 25.206545888263843 112-113 22.23543834142584 26.637392016487983 25.76007873500526 25.367090907080918 114-115 22.520243338373003 25.510162006465915 26.100460741369314 25.869133913791764 116-117 23.429438570450284 25.02261756652911 25.50780470373677 26.040139159283836 118-119 22.4619053698919 25.483402100665327 26.00015129909409 26.054541230348683 120-121 21.615727504339088 25.61384397088531 26.831716862853128 25.938711661922476 122-123 22.199291439819742 26.213469814481773 27.207766732690192 24.379472013008296 124-125 22.72032609262176 27.056994917982603 25.09535896727751 25.127320022118127 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13.0 1 18.5 2 22.0 3 34.0 4 50.5 5 55.5 6 57.5 7 65.0 8 76.0 9 111.0 10 138.0 11 147.5 12 196.0 13 261.0 14 340.0 15 409.0 16 488.5 17 612.5 18 778.5 19 1010.0 20 1258.5 21 1555.5 22 1930.0 23 2377.0 24 2846.5 25 3486.5 26 4419.5 27 5414.0 28 6170.0 29 7262.0 30 8831.0 31 10133.5 32 11691.5 33 13414.0 34 15537.5 35 17395.5 36 19321.0 37 22047.5 38 23778.0 39 24837.0 40 26043.0 41 27937.0 42 30139.5 43 33654.0 44 37229.5 45 41724.0 46 55040.0 47 76107.0 48 94066.0 49 108550.5 50 111955.5 51 98667.5 52 83012.5 53 73116.0 54 67725.0 55 63074.5 56 57751.5 57 53303.5 58 50172.0 59 45874.5 60 41462.0 61 35454.5 62 27867.5 63 21729.5 64 17424.0 65 15195.0 66 13099.0 67 11132.5 68 9436.0 69 8083.0 70 7058.0 71 5342.5 72 3758.5 73 2815.5 74 2256.5 75 1781.0 76 1278.5 77 1026.5 78 803.0 79 525.0 80 528.5 81 593.5 82 497.0 83 268.5 84 92.5 85 53.0 86 42.5 87 31.5 88 23.5 89 20.0 90 13.5 91 11.0 92 13.0 93 11.5 94 8.0 95 4.5 96 1.5 97 4.0 98 3.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.157157727798035 2 0.018823073861741833 3 0.00950117061592683 4 0.01720966753073539 5 0.0037048589823110915 6 0.0 7 0.0 8 2.3902316014910266E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.262147455777729E-4 28-29 0.007529229544696733 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.987789501863783E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0014938947509318917 66-67 5.9755790037275664E-5 68-69 0.005826189528634377 70-71 0.01858405070159273 72-73 1.1951158007455133E-4 74-75 0.008425566395255869 76-77 4.780463202982053E-4 78-79 0.0010457263256523242 80-81 8.664589555404971E-4 82-83 0.0 84-85 0.01888282965177911 86-87 0.0025694989716028536 88-89 0.018823073861741833 90-91 2.9877895018637832E-5 92-93 0.014251755923890247 94-95 0.013893221183666591 96-97 0.006035334793764842 98-99 0.002629254761640129 100-101 6.274357953913944E-4 102-103 0.0 104-105 3.2865684520501614E-4 106-107 9.560926405964106E-4 108-109 0.0 110-111 0.0 112-113 9.859705356150485E-4 114-115 0.005258509523280258 116-117 0.0 118-119 0.07729411441321607 120-121 0.0016432842260250807 122-123 0.005049364258149793 124-125 0.254499909768757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1673478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.04224101848772 #Duplication Level Percentage of deduplicated Percentage of total 1 79.49274377390371 41.36980530702387 2 11.75909182286006 12.239389816076226 3 3.401001491450869 5.309872179669669 4 1.5419155823659894 3.209789694706107 5 0.8730486585099885 2.2717704353522103 6 0.5395337541839944 1.6847127403711768 7 0.3849800678430602 1.4024657834601606 8 0.2818815062897244 1.1735796231187359 9 0.2087026370689757 0.9775217645583845 >10 1.3141342743466549 12.93400547968668 >50 0.12183315156471977 4.423890794989827 >100 0.07329675531345113 7.061289034540996 >500 0.0049554491890328204 1.8499301737216634 >1k 0.002304860087922242 2.1226366901720572 >5k 5.762150219805605E-4 1.9693404825522625 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 7599 0.45408424849325774 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 7451 0.445240391567741 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6513 0.38918946051277636 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 5681 0.339472643201763 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 5592 0.3341543778884455 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4266 0.25491820029901796 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 3867 0.23107564007414497 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 2292 0.13696027076543582 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2191 0.13092493597167099 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1971 0.11777866216347033 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1878 0.1122213736900037 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1842 0.11007016524866176 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1805 0.10785920101728257 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1675 0.10009094831243673 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.963368505591349E-5 0.0 12-13 0.0 0.0 0.0 1.7926737011182697E-4 0.0 14-15 0.0 0.0 0.0 2.3902316014910266E-4 0.0 16-17 0.0 0.0 0.0 2.3902316014910266E-4 0.0 18-19 0.0 0.0 0.0 4.1829053026092966E-4 0.0 20-21 0.0 0.0 0.0 8.067031655032215E-4 0.0 22-23 0.0 0.0 0.0 0.0017329179110809941 0.0 24-25 0.0 0.0 0.0 0.0031670568719756103 0.0 26-27 0.0 0.0 0.0 0.00495973057309388 0.0 28-29 0.0 0.0 0.0 0.01266822748790244 0.0 30-31 0.0 0.0 0.0 0.03958821089969512 0.0 32-33 0.0 0.0 0.0 0.08646662818393788 0.0 34-35 0.0 0.0 0.0 0.14529620347563577 0.0 36-37 0.0 0.0 0.0 0.23609512643727615 0.0 38-39 0.0 0.0 0.0 0.3810327951726883 0.0 40-41 0.0 0.0 0.0 0.566006843233075 0.0 42-43 0.0 0.0 0.0 0.8320993762690636 0.0 44-45 0.0 0.0 0.0 1.174589686867709 0.0 46-47 0.0 0.0 0.0 1.5344091765771646 0.0 48-49 0.0 0.0 0.0 1.7773463409737087 0.0 50-51 0.0 0.0 0.0 2.061186343650768 0.0 52-53 0.0 0.0 0.0 2.3151783292042083 0.0 54-55 0.0 0.0 0.0 2.5739806558556495 0.0 56-57 0.0 0.0 0.0 2.9082246674291508 0.0 58-59 0.0 0.0 0.0 3.2882117362761862 0.0 60-61 0.0 0.0 0.0 3.745941087961718 0.0 62-63 0.0 0.0 0.0 4.23611783363749 0.0 64-65 0.0 0.0 0.0 4.702272751718278 0.0 66-67 0.0 0.0 0.0 5.1480509453963545 0.0 68-69 0.0 0.0 0.0 5.58946696640171 0.0 70-71 0.0 0.0 0.0 6.082900402634513 0.0 72-73 0.0 0.0 0.0 6.569222899852882 0.0 74-75 0.0 0.0 0.0 7.036901590579619 0.0 76-77 0.0 0.0 0.0 7.548978833304053 0.0 78-79 0.0 0.0 0.0 8.170797584431943 0.0 80-81 0.0 0.0 0.0 9.208905046854515 0.0 82-83 0.0 0.0 0.0 10.050714739004636 0.0 84-85 0.0 0.0 0.0 10.62069534227519 0.0 86-87 0.0 2.9877895018637832E-5 0.0 11.152462117816906 0.0 88-89 0.0 5.9755790037275664E-5 0.0 11.745418822356793 0.0 90-91 0.0 5.9755790037275664E-5 0.0 12.413727578133683 0.0 92-93 0.0 5.9755790037275664E-5 0.0 13.149440865072622 0.0 94-95 0.0 5.9755790037275664E-5 0.0 13.928746000843752 0.0 96-97 0.0 5.9755790037275664E-5 0.0 14.733656492645855 0.0 98-99 0.0 5.9755790037275664E-5 0.0 15.566084525760123 0.0 100-101 0.0 5.9755790037275664E-5 0.0 16.44198489612651 0.0 102-103 0.0 5.9755790037275664E-5 0.0 17.294819531538508 0.0 104-105 0.0 5.9755790037275664E-5 0.0 18.155780954395578 0.0 106-107 0.0 5.9755790037275664E-5 0.0 18.986266924333634 0.0 108-109 0.0 5.9755790037275664E-5 0.0 19.755533087378502 0.0 110-111 0.0 5.9755790037275664E-5 0.0 20.621185339753495 0.0 112-113 0.0 5.9755790037275664E-5 0.0 22.174746247037607 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 1725 0.0 64.5993 1 AGTAGTA 1285 0.0 64.36318 2 GAGTACG 760 0.0 61.15842 1 GAGTCAG 4105 0.0 60.96933 1 GAGTAAG 1300 0.0 58.67349 1 GGGGTAA 5330 0.0 57.364723 9 AGGGTAA 4500 0.0 56.18048 6 GTGGGGT 4610 0.0 56.001263 7 TGGGGTA 4635 0.0 55.699203 8 GATAGCG 1315 0.0 53.830723 9 AGTACGG 520 0.0 53.779938 2 GATTGTG 4105 0.0 53.036755 9 GAGTCCG 870 0.0 50.68597 1 GTTAACG 130 0.0 50.342667 3 GTAGTAG 2690 0.0 49.843155 1 GAGTCGT 780 0.0 49.658546 1 TCGTAGG 865 0.0 49.52702 2 GAGTTAG 1230 0.0 49.416313 1 GTAAGGG 950 0.0 48.849247 3 GGTATCA 3670 0.0 48.711384 1 >>END_MODULE