FastQCFastQC Report
Thu 26 May 2016
SRR935983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673478
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT77830.46507931386011647No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT69540.41554176391921493No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC62510.3735334435230102No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG60320.3604469255048468No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT58630.3503481969885472No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT47100.28144977107556834No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA31970.1910392607491703No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC22450.13415174863368387No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC20310.12136400956570687No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG20030.11969084744466316No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG19830.11849573164391765No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC19290.11526891898190475No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA18630.11132503683944457No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG17870.10678359679661162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT17400.063.8420831
GATTGTG41100.060.0738569
GGTATCA43900.059.8592831
GAGTACG7550.057.7456471
GAGTCAG43050.057.1567341
AGGGTAA45950.056.5815966
GAGTAAG12300.055.8388021
GATAGCG14500.055.3917359
AGTAGTA13650.055.0103532
TAGTAGG26550.054.0952382
GAGTCGA5150.052.1853521
GTAGTAG28600.050.7438741
TGGGGTA45950.050.6256378
GGGGTAA52250.050.328649
GAGTCGG7750.050.0904581
GAGTTAG12400.048.6455421
TCGTAGG9150.048.196682
GAGTCCG6100.047.9744071
AGTACGG5050.047.2035982
GGGATTG53600.046.841087
Produced by FastQC (version 0.11.5)