FastQCFastQC Report
Thu 26 May 2016
SRR935982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454890
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT11660.25632570511552244No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA11580.2545670381850557No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT10900.23961836927608873No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA8980.19741036294488776No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC8240.18114269383807075No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC7570.16641385829541208No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA5090.11189518345094418No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTA152.7023468E-4118.98127
TTCGTAC152.7023468E-4118.98128
TATTATG300.004235713759.4971432
GTATAAA1152.728484E-1046.5988431
GTGGAAT2350.045.6073761
GGACTGG705.4518358E-542.493298
AGGGGTT853.4800141E-641.9933626
TGGAATC2600.041.190332
GTATTAG758.1461134E-539.695311
CTAGGGT905.150305E-639.664764
GTATTGG2500.038.10751
TGTAAGG957.4647724E-637.577142
GTCATAG1001.0555414E-535.725781
GTGTAAG1001.0555414E-535.725781
AGTTAGA851.7093448E-434.9983142
GTCTTGG1407.996459E-834.024551
TCGGGAC1051.4822084E-533.9983673
GCAGAGA8400.033.9983673
TAGAGGC350.002790842233.9946324-25
ACGGGAT1051.4833247E-533.994635