##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935982_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 454890 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.401694915254236 31.0 30.0 34.0 12.0 34.0 2 28.36530589812922 31.0 30.0 34.0 10.0 34.0 3 28.34014816768889 31.0 30.0 34.0 10.0 34.0 4 31.348882147332322 37.0 33.0 37.0 7.0 37.0 5 31.601769658598784 37.0 33.0 37.0 10.0 37.0 6 31.778665171799776 37.0 35.0 37.0 8.0 37.0 7 31.771773395766008 37.0 35.0 37.0 8.0 37.0 8 31.478135373386973 37.0 35.0 37.0 8.0 37.0 9 32.594877882565015 39.0 34.0 39.0 7.0 39.0 10-11 32.69365670821517 39.0 34.0 39.0 7.0 39.0 12-13 32.7005715667524 39.0 34.0 39.0 7.0 39.0 14-15 33.71882982699114 40.0 33.0 41.0 8.0 41.0 16-17 33.891529820396144 39.5 33.5 41.0 8.5 41.0 18-19 34.10949899975819 40.0 33.5 41.0 11.0 41.0 20-21 34.31418364879421 40.0 34.0 41.0 12.0 41.0 22-23 34.079612653608564 40.0 33.0 41.0 8.5 41.0 24-25 33.8222713183407 40.0 33.0 41.0 3.5 41.0 26-27 33.630911868803445 39.0 33.0 41.0 2.0 41.0 28-29 33.356072896744266 39.0 33.0 41.0 2.0 41.0 30-31 33.068261557739234 39.0 32.0 41.0 2.0 41.0 32-33 32.708928532172614 39.0 32.0 41.0 2.0 41.0 34-35 32.33541405614544 39.0 32.0 41.0 2.0 41.0 36-37 32.119440963749476 39.0 32.0 41.0 2.0 41.0 38-39 31.95340961551144 39.0 32.0 41.0 2.0 41.0 40-41 31.688111411550047 39.0 32.0 41.0 2.0 41.0 42-43 31.388246609070325 38.0 31.0 41.0 2.0 41.0 44-45 31.046444195299962 37.5 31.0 41.0 2.0 41.0 46-47 30.676532788146584 37.0 31.0 40.0 2.0 41.0 48-49 30.276570159818856 36.5 30.0 40.0 2.0 41.0 50-51 29.900430873397966 36.0 30.0 39.0 2.0 41.0 52-53 29.554220800633118 35.0 30.0 39.0 2.0 41.0 54-55 29.187129855569477 35.0 29.0 38.5 2.0 41.0 56-57 28.848860163995692 35.0 29.0 37.0 2.0 40.0 58-59 28.509714436457166 35.0 28.5 37.0 2.0 39.5 60-61 28.199097584031307 35.0 28.0 36.0 2.0 39.0 62-63 27.902220316999713 35.0 27.0 35.5 2.0 39.0 64-65 27.662478841038492 35.0 27.0 35.0 2.0 37.0 66-67 27.48211985315131 35.0 27.5 35.0 2.0 37.0 68-69 27.34336982567214 35.0 27.0 35.0 2.0 36.0 70-71 27.126041460572885 35.0 26.0 35.0 2.0 36.0 72-73 26.907889819516807 35.0 25.5 35.0 2.0 35.5 74-75 26.664257292971925 35.0 25.0 35.0 2.0 35.0 76-77 26.415896150717757 34.5 24.0 35.0 2.0 35.0 78-79 26.199514168260457 34.0 21.5 35.0 2.0 35.0 80-81 25.91471564554068 34.0 18.5 35.0 2.0 35.0 82-83 25.572632944228275 34.0 13.5 35.0 2.0 35.0 84-85 25.249440524082743 34.0 4.5 35.0 2.0 35.0 86-87 24.94191013211985 34.0 2.0 35.0 2.0 35.0 88-89 24.559869418980412 34.0 2.0 35.0 2.0 35.0 90-91 24.168250566070917 33.0 2.0 35.0 2.0 35.0 92-93 23.729178482710104 33.0 2.0 35.0 2.0 35.0 94-95 23.29937897074018 33.0 2.0 35.0 2.0 35.0 96-97 22.88976126096419 32.0 2.0 35.0 2.0 35.0 98-99 22.45523972828596 31.0 2.0 35.0 2.0 35.0 100-101 22.08394996592583 31.0 2.0 35.0 2.0 35.0 102-103 21.314056145441754 29.0 2.0 34.5 2.0 35.0 104-105 21.57597111389567 30.0 2.0 35.0 2.0 35.0 106-107 21.462060058475675 30.0 2.0 35.0 2.0 35.0 108-109 21.16988942381675 30.0 2.0 35.0 2.0 35.0 110-111 20.76389566708435 29.0 2.0 35.0 2.0 35.0 112-113 20.32351667436083 29.0 2.0 35.0 2.0 35.0 114-115 19.76433863131746 28.0 2.0 35.0 2.0 35.0 116-117 19.11568620985293 26.0 2.0 35.0 2.0 35.0 118-119 18.28774209149465 24.5 2.0 34.0 2.0 35.0 120-121 17.36122249335004 13.5 2.0 34.0 2.0 35.0 122-123 16.468718811141155 2.0 2.0 34.0 2.0 35.0 124-125 14.262450262700874 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5879520488370673 1101 2 0.5849586511691776 1101 3 0.6584345236351794 1101 4 0.47262361785746876 1101 5 0.4597078031245374 1101 6 0.7007901203720444 1101 7 0.5237314211057758 1101 8 0.6390375167374955 1101 9 0.6106798466436061 1101 10-11 0.6182615483862861 1101 12-13 0.4795625214453523 1101 14-15 0.6483654172634346 1101 16-17 0.4746333826301239 1101 18-19 0.5240991109483275 1101 20-21 0.2433061253458817 1101 22-23 0.6695368669973973 1101 24-25 0.7411399677880368 1101 26-27 0.5454136871735287 1101 28-29 0.644410095101442 1101 30-31 0.5833940489962188 1101 32-33 0.7116029629149097 1101 34-35 0.6226292568871372 1101 36-37 0.8392668994788792 1101 38-39 1.2039710662175445 1101 40-41 1.2208986273706408 1101 42-43 1.1621814579209229 1101 44-45 1.171394472528629 1101 46-47 1.168874659894069 1101 48-49 1.2801883504395803 1101 50-51 1.3776810428841628 1101 52-53 1.4255408789723276 1101 54-55 1.1128319426411153 1101 56-57 1.2232570418055992 1101 58-59 0.8997817180143741 1101 60-61 0.9188174628043555 1101 62-63 0.9437386882432648 1101 64-65 0.8965492056929385 1101 66-67 0.6062980392263206 1101 68-69 0.7925260134790797 1101 70-71 0.7114554784741003 1101 72-73 0.7700207651272741 1101 74-75 0.6536078364058042 1101 76-77 0.8302744216472036 1101 78-79 0.6927277094104518 1101 80-81 0.5143707653726715 1101 82-83 0.48687222371158256 1101 84-85 0.4336595693999925 1101 86-87 0.42577252741679317 1101 88-89 0.40655072174512696 1101 90-91 0.2645966329613856 1101 92-93 0.22601125688110812 1101 94-95 0.13578397638743667 1101 96-97 0.12491785774781405 1101 98-99 0.3010543699991075 1101 100-101 0.5193743057157505 1101 102-103 0.6399800015949673 1101 104-105 0.4732036570918865 1101 106-107 0.5867334751290088 1101 108-109 0.6577310044924403 1101 110-111 0.9105052019567168 1101 112-113 0.7756925531058947 1101 114-115 0.9556664837086473 1101 116-117 0.7994915573770989 1101 118-119 0.3501433442820563 1101 120-121 0.08133801868731894 1101 122-123 0.0949875498922772 1101 124-125 0.4205665719397711 1102 1 0.3945659549987397 1102 2 0.5227847030144517 1102 3 0.43550523886304404 1102 4 0.28441935933099316 1102 5 0.5170256313298722 1102 6 0.34267819272930566 1102 7 -0.0783251618439671 1102 8 0.03232131980083608 1102 9 0.1546514205756111 1102 10-11 0.18829169724628514 1102 12-13 0.24163283289383486 1102 14-15 0.19046210896442517 1102 16-17 0.369958963399462 1102 18-19 0.29027316677756687 1102 20-21 0.19648656088923389 1102 22-23 0.23305943826445485 1102 24-25 0.16725507182852084 1102 26-27 0.21393370117122146 1102 28-29 0.25176022565677414 1102 30-31 0.1044511433998423 1102 32-33 0.3198992831388736 1102 34-35 0.5628577960133612 1102 36-37 0.3327633596796957 1102 38-39 0.4036493886488337 1102 40-41 0.4955789686988048 1102 42-43 0.12679961722535182 1102 44-45 0.37754672427160685 1102 46-47 0.4788910802553161 1102 48-49 0.39396934050660803 1102 50-51 0.16486104772951649 1102 52-53 0.20578933788104337 1102 54-55 0.23683528055061487 1102 56-57 0.38863026854117777 1102 58-59 0.3047576258450029 1102 60-61 0.21444722403615302 1102 62-63 0.1939432697932375 1102 64-65 0.1881869348377343 1102 66-67 0.25447834017654714 1102 68-69 0.4283573952120996 1102 70-71 0.38136718538411074 1102 72-73 0.15574716422281298 1102 74-75 0.15632796346171318 1102 76-77 0.3707936819233879 1102 78-79 0.48877874712344394 1102 80-81 0.4964268099499165 1102 82-83 0.48921549001753917 1102 84-85 0.7365963913871241 1102 86-87 0.7554664626505811 1102 88-89 0.8106827306821103 1102 90-91 0.7605628770056398 1102 92-93 0.8826076659365327 1102 94-95 0.8194430250832276 1102 96-97 0.46171023439321957 1102 98-99 0.6251573068210945 1102 100-101 0.6986684993683383 1102 102-103 0.7039978755619636 1102 104-105 0.4583809162374948 1102 106-107 0.211267109950942 1102 108-109 -0.00591000872460512 1102 110-111 -0.021758116032998487 1102 112-113 0.17463868893727863 1102 114-115 -0.03660652498606609 1102 116-117 0.03804575802853094 1102 118-119 0.030973245490702794 1102 120-121 -0.21869832145642576 1102 122-123 -0.2298691084296145 1102 124-125 -0.12908483186652298 1103 1 0.17117694582253762 1103 2 0.10696348433211256 1103 3 -0.08629082258659082 1103 4 -0.18260301410956714 1103 5 0.03521822850484213 1103 6 -0.04465555301767665 1103 7 0.19217179660535777 1103 8 0.10976744826768936 1103 9 0.21478648604627892 1103 10-11 0.1904259378896498 1103 12-13 -0.008962589314634783 1103 14-15 0.4303347762497083 1103 16-17 0.25374079657813553 1103 18-19 0.1048958082869973 1103 20-21 0.4097869011305022 1103 22-23 0.529940560029587 1103 24-25 0.5880200991509241 1103 26-27 -0.03159161071661032 1103 28-29 -0.05518636677543043 1103 30-31 -0.06941987423084583 1103 32-33 -0.04918258097082884 1103 34-35 -0.05965998932533978 1103 36-37 0.16141579807541007 1103 38-39 -0.01033401170171544 1103 40-41 0.11395715201667045 1103 42-43 0.173598256260302 1103 44-45 0.09093470241367996 1103 46-47 0.11536906767669919 1103 48-49 0.02071178818162167 1103 50-51 -0.1256921020797428 1103 52-53 -0.0018300043191814552 1103 54-55 -0.022647655834578728 1103 56-57 -0.06574131615827383 1103 58-59 -0.1726189078182614 1103 60-61 -0.015109946806202146 1103 62-63 -0.11464266690126479 1103 64-65 -0.13839828013287914 1103 66-67 -0.07447309811606218 1103 68-69 -0.0036330161705144803 1103 70-71 0.036055813077688015 1103 72-73 0.14802987378049792 1103 74-75 0.11948911409579921 1103 76-77 0.18774646056036204 1103 78-79 0.19345190930511436 1103 80-81 0.3649416052896406 1103 82-83 0.4351325948737106 1103 84-85 0.5482341890982276 1103 86-87 0.5343560388955524 1103 88-89 0.7140258588396549 1103 90-91 0.72460298423319 1103 92-93 0.5933742398101316 1103 94-95 0.7472061965442016 1103 96-97 0.764022328423529 1103 98-99 0.9590345106565401 1103 100-101 0.6356877797065188 1103 102-103 0.5761032472497654 1103 104-105 0.4863631824505035 1103 106-107 0.4579110938668087 1103 108-109 0.5654865270007292 1103 110-111 0.4995353162796121 1103 112-113 -0.04640987277011277 1103 114-115 0.029194073013727007 1103 116-117 0.1406671622970066 1103 118-119 0.10292396205941756 1103 120-121 0.37206361276359345 1103 122-123 0.2500360364306786 1103 124-125 0.30223540733339505 1104 1 0.10287299212129497 1104 2 0.14554824235614916 1104 3 0.07845657163197117 1104 4 0.03578804503355215 1104 5 0.11528879867639574 1104 6 0.23999469648774152 1104 7 0.21600768404740833 1104 8 -0.19023698082257567 1104 9 -0.020494170640169784 1104 10-11 -0.04049944670302352 1104 12-13 -0.11529397384825302 1104 14-15 0.013575836869193836 1104 16-17 0.042642972737816365 1104 18-19 -0.29360789738519344 1104 20-21 -0.2720272542419906 1104 22-23 -0.3344333142433058 1104 24-25 -0.11737896188193275 1104 26-27 0.07971142763931738 1104 28-29 0.18041539895521908 1104 30-31 0.02994307495272608 1104 32-33 0.005535138342956714 1104 34-35 0.11900213014199323 1104 36-37 0.12249885828150298 1104 38-39 -0.04859027886444167 1104 40-41 0.20548430233999682 1104 42-43 0.2521850811931259 1104 44-45 0.13944800444810923 1104 46-47 0.1294388110847393 1104 48-49 0.263274607445215 1104 50-51 0.2922257825867476 1104 52-53 0.1775702968589563 1104 54-55 0.2681701018646585 1104 56-57 0.2284683865021293 1104 58-59 0.35292587028431655 1104 60-61 0.37425506928615704 1104 62-63 0.2696288953865107 1104 64-65 0.16410858495703096 1104 66-67 0.14495141542219514 1104 68-69 0.10115556858750807 1104 70-71 0.17449810203153504 1104 72-73 0.03886756238592426 1104 74-75 0.08753322783483952 1104 76-77 0.190998150996478 1104 78-79 0.29403285831152104 1104 80-81 0.2806080357381582 1104 82-83 0.3215854841169268 1104 84-85 0.4755122178577871 1104 86-87 0.27303233145173067 1104 88-89 0.34491654889191636 1104 90-91 0.3720806173273523 1104 92-93 0.45248118757639233 1104 94-95 0.25700555875520337 1104 96-97 0.2411940590167312 1104 98-99 0.4678520911921673 1104 100-101 0.6619153758915957 1104 102-103 0.7339191152038218 1104 104-105 0.49314707237284594 1104 106-107 0.5952867102485371 1104 108-109 0.6606566040669009 1104 110-111 0.5859062673529252 1104 112-113 0.4638213257417121 1104 114-115 0.3592720250023902 1104 116-117 0.2721705431692456 1104 118-119 0.2854910627769307 1104 120-121 0.1522847720041547 1104 122-123 -0.23893552880981872 1104 124-125 0.06670007795671573 1105 1 0.206152165368664 1105 2 0.2487120279370174 1105 3 0.15236958375488996 1105 4 0.3915282208555091 1105 5 0.3452419945541685 1105 6 0.4552792352616031 1105 7 0.12635867890544716 1105 8 0.4238778154821361 1105 9 0.37189601275409956 1105 10-11 0.4211377600500441 1105 12-13 0.31173053881915536 1105 14-15 0.44101862982766704 1105 16-17 0.3390594645200551 1105 18-19 0.3119321529526786 1105 20-21 0.18721205256660767 1105 22-23 0.47437292822945665 1105 24-25 0.33802009915093123 1105 26-27 0.4808710736876449 1105 28-29 0.34227544813921185 1105 30-31 0.5518775491816115 1105 32-33 0.7116824162757851 1105 34-35 0.5276295217316758 1105 36-37 0.5917696803465162 1105 38-39 0.7642761671233167 1105 40-41 0.9057071447709255 1105 42-43 0.8956948855389157 1105 44-45 0.8159731048714391 1105 46-47 0.6536990679665315 1105 48-49 0.7342113389453253 1105 50-51 0.9092505116060252 1105 52-53 0.6981453601418792 1105 54-55 0.5167728293406242 1105 56-57 0.535641172320986 1105 58-59 0.545684573038475 1105 60-61 0.40043725198810876 1105 62-63 0.524754631884349 1105 64-65 0.32859009768933944 1105 66-67 0.4824567340724464 1105 68-69 0.5874568890551188 1105 70-71 0.6502234216914289 1105 72-73 0.7382394207764129 1105 74-75 0.7207194418933867 1105 76-77 0.6929525295978323 1105 78-79 0.8622017281614411 1105 80-81 1.0002015826829123 1105 82-83 1.0698745013745956 1105 84-85 1.093784576021104 1105 86-87 0.9300100399969082 1105 88-89 1.0169379244571353 1105 90-91 1.0974002772222065 1105 92-93 1.2674859112589871 1105 94-95 1.0034411616197048 1105 96-97 1.098221528493088 1105 98-99 1.142094679337525 1105 100-101 1.3780797456225287 1105 102-103 1.391343950956692 1105 104-105 1.4359400308404986 1105 106-107 1.3625425751872733 1105 108-109 1.4058373660582006 1105 110-111 1.338944208260017 1105 112-113 1.208104227453056 1105 114-115 1.2374875547399533 1105 116-117 1.1516553372383207 1105 118-119 0.8960144179618048 1105 120-121 0.9616282882990568 1105 122-123 1.0614582316021135 1105 124-125 0.9388938138487699 1106 1 0.689000616837486 1106 2 0.7133132117319931 1106 3 0.7668809487908739 1106 4 0.18693665800910253 1106 5 0.9182880008147549 1106 6 0.7593221251714546 1106 7 0.627763476830669 1106 8 0.9929894579918539 1106 9 1.5407529557557922 1106 10-11 1.3888459989749151 1106 12-13 1.1631786774909472 1106 14-15 1.2804487164394374 1106 16-17 1.2669592858120993 1106 18-19 0.9681854209379281 1106 20-21 1.0782280851569865 1106 22-23 1.038577654578539 1106 24-25 1.0873897411075575 1106 26-27 1.1989005918733326 1106 28-29 1.2747370863497167 1106 30-31 1.2549992448854113 1106 32-33 1.3744763025580369 1106 34-35 1.32088835081111 1106 36-37 1.4167369714452747 1106 38-39 1.25661310482651 1106 40-41 1.356088380667753 1106 42-43 1.294084654798823 1106 44-45 1.5029367195594254 1106 46-47 1.5171664206486604 1106 48-49 1.4294062096318747 1106 50-51 1.4409938321346658 1106 52-53 1.512501721006462 1106 54-55 1.3577425001136234 1106 56-57 1.3399571205537768 1106 58-59 1.4638298214750733 1106 60-61 1.394424159132008 1106 62-63 1.3378223898547468 1106 64-65 1.40978486147646 1106 66-67 1.3472649774127277 1106 68-69 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-2.967304618783544 2216 76-77 -2.980792277993867 2216 78-79 -3.3742125556770084 2216 80-81 -3.3758111062811516 2216 82-83 -3.378850662083355 2216 84-85 -3.3956388556266894 2216 86-87 -3.523342176715925 2216 88-89 -3.3415805431128476 2216 90-91 -3.293009425416674 2216 92-93 -2.933354253230668 2216 94-95 -2.838302153643113 2216 96-97 -3.0809861199008886 2216 98-99 -3.0110795417230705 2216 100-101 -3.0738541333696077 2216 102-103 -3.043173866324725 2216 104-105 -3.311544790548993 2216 106-107 -3.1146420921755364 2216 108-109 -3.0479546291396673 2216 110-111 -3.321679151689292 2216 112-113 -3.662958348231573 2216 114-115 -3.3308688200680336 2216 116-117 -3.3825817304604726 2216 118-119 -3.3232641368969134 2216 120-121 -2.9611822505442227 2216 122-123 -2.437994650614371 2216 124-125 -2.0791371040377076 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35262.0 3 3661.0 4 1945.0 5 4128.0 6 11646.0 7 9991.0 8 6186.0 9 6034.0 10 4521.0 11 2923.0 12 2161.0 13 1792.0 14 1986.0 15 2044.0 16 1708.0 17 1762.0 18 2001.0 19 2475.0 20 3031.0 21 3809.0 22 4737.0 23 5649.0 24 6564.0 25 7090.0 26 7122.0 27 7748.0 28 8903.0 29 10995.0 30 14787.0 31 19192.0 32 25026.0 33 31255.0 34 39948.0 35 51111.0 36 76042.0 37 27530.0 38 2125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.472826063026446 18.711995455966466 11.89154202130236 12.923636459704726 2 20.401037773184996 24.684710434897323 34.44615452266831 20.468097269249373 3 30.883908058612086 25.150212705147794 24.049642193666113 19.916237042574007 4 16.01341175177266 23.712856593195188 28.350464464354424 31.923267190677734 5 15.987759220705561 35.63785935854623 25.08216579133077 23.29221562941744 6 30.806568620985292 37.45147178438744 8.927213172415309 22.81474642221196 7 28.000615533425666 42.52258787838818 11.32977203279914 18.147024555387016 8 34.79082855195762 29.69706962122711 10.516388577458287 24.99571324935699 9 35.78997120182901 18.660555299083295 14.59825452307151 30.951218976016182 10-11 29.90272373540856 28.853129327969402 14.400844160126624 26.843302776495413 12-13 32.311547846732175 30.848996460682805 16.538283980742598 20.30117171184242 14-15 35.63158126140385 24.26861439029216 20.25017037085889 19.849633977445098 16-17 29.751148629338964 25.068148343555585 21.556420233463037 23.62428279364242 18-19 28.508980193013695 25.806128954252678 21.327353865769748 24.357536986963883 20-21 37.982699114071536 23.53788828068324 17.44553628349711 21.033876321748117 22-23 33.47512585460221 26.857482028622304 16.841434192881795 22.82595792389369 24-25 26.368023038536787 29.10066169843259 18.78223306733496 25.74908219569566 26-27 23.64613835615234 29.007285373853552 20.942921711908973 26.403654558085137 28-29 25.22006771314251 27.701710416391855 22.702589807852966 24.375632062612674 30-31 26.13258150322056 28.875882081382315 19.962958077777046 25.028578337620083 32-33 23.236057068741893 29.016905185869113 21.420123546351867 26.326914199037134 34-35 22.380575523752995 29.833036558288818 21.443755633230012 26.342632284728175 36-37 22.741431994548133 31.190507595242806 19.605179274110224 26.462881136098837 38-39 23.55921211721515 28.311240080019346 20.88746729978676 27.242080502978745 40-41 22.061157642506977 28.635274461957838 22.386511024643323 26.917056870891866 42-43 21.86979269713557 28.809932071489815 24.09230803051287 25.227967200861745 44-45 22.0603882257249 28.040735122776933 24.610675108267934 25.288201543230233 46-47 19.892061817142608 28.348611752291763 24.178372793422586 27.580953637143047 48-49 19.208160214557367 28.655718965024512 25.187847611510477 26.948273208907647 50-51 19.94548132515553 28.387632174811493 24.048890940666976 27.617995559365998 52-53 20.1647651080481 27.385082107762315 23.126360218954034 29.32379256523555 54-55 19.007342434434698 26.77328584932621 23.280573325419333 30.93879839081976 56-57 19.473893052912967 25.767413333157467 24.464786425684537 30.293907188245033 58-59 20.9530875596298 24.24014596935523 23.85104091098947 30.9557255600255 60-61 20.67862560179384 24.036360438787398 23.64945371408472 31.635560245334037 62-63 20.09628701444305 23.19516806260854 24.37413440612016 32.33441051682824 64-65 19.869724742406287 22.503478916556567 24.86562478703315 32.761171554004 66-67 20.538371914089122 23.137461803952604 24.464376002989734 31.859790278968543 68-69 19.885196328129208 22.732314199754434 25.427078673052467 31.955410799063895 70-71 19.155768486596777 21.928977872370364 26.967608879195105 31.947644761837758 72-73 19.727516542460815 20.875926048055575 28.043812789905253 31.35274461957836 74-75 19.985028129967265 19.8810384893581 28.652223905305668 31.481709475368962 76-77 19.864098564813133 19.299015033354326 29.24650766066849 31.590378741164045 78-79 20.73040438792222 19.644415621530715 30.35591413214329 29.26926585840377 80-81 21.785114446760055 20.759468347974426 29.762752701787708 27.69266450347781 82-83 21.128074919211237 20.603332673833236 29.648046780540348 28.62054562641518 84-85 21.237423264322526 20.424552778315245 29.489147068653143 28.84887688870908 86-87 21.97440432288409 20.195699319493098 29.17825851348098 28.651637844141824 88-89 22.28228426312231 20.0749767758887 28.859242646614224 28.783496314374762 90-91 23.192639978895997 20.19004594517356 28.15636747345512 28.460946602475325 92-93 23.785150495789637 20.97333069498494 27.58272321526724 27.658795593958178 94-95 22.89169606432343 21.92935108222586 27.04426852876866 28.13468432468205 96-97 22.49031300667784 22.75390914836901 26.01225645112534 28.743521393827802 98-99 22.097255183011615 23.821133474983814 25.607006752397083 28.474604589607488 100-101 21.66314380212163 25.575447008093242 25.541922088085904 27.21948710169922 102-103 21.225900767218448 27.531820879773132 25.377893556684032 25.864384796324387 104-105 20.184880877139953 29.424967711796874 25.159957132257976 25.2301942788052 106-107 19.246466402936136 30.733253679238747 24.851637836940668 25.168642080884446 108-109 19.079447778583834 32.35848227043901 24.70762162280991 23.854448328167248 110-111 18.78926773505683 34.50207742531161 23.645276880124865 23.063377959506695 112-113 18.596450731782383 35.87135139294214 23.020230499084928 22.511967376190555 114-115 18.291320960458684 36.624412464330156 23.21353981172477 21.870726763486388 116-117 17.837499175624878 37.40200927696806 22.93147793972169 21.829013607685376 118-119 17.690583354231837 38.17012701890545 22.676306376463444 21.46298325039927 120-121 17.966476909869737 37.939634695266115 22.396101833338648 21.6977865615255 122-123 18.430991916318394 38.18577738108062 22.272554395020965 21.110676307580018 124-125 18.438403497637683 40.39383682391933 21.25632448346379 19.911435194979195 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 7.0 2 7.5 3 9.5 4 10.5 5 13.5 6 13.5 7 11.0 8 13.5 9 14.0 10 15.5 11 20.0 12 23.0 13 33.0 14 49.0 15 62.5 16 75.5 17 99.0 18 143.5 19 209.5 20 282.5 21 402.0 22 574.0 23 811.0 24 1115.5 25 1484.0 26 1856.0 27 2215.0 28 2624.0 29 3005.0 30 3348.0 31 3670.0 32 3894.5 33 3941.5 34 3978.5 35 4089.5 36 3945.5 37 3712.0 38 3492.5 39 3223.5 40 3013.0 41 2911.0 42 2708.0 43 2466.5 44 2478.0 45 2662.0 46 3433.5 47 4628.0 48 6667.0 49 11356.5 50 19142.0 51 29772.0 52 40912.0 53 47206.5 54 46073.0 55 40879.0 56 33921.0 57 25824.5 58 19004.5 59 13879.0 60 10169.5 61 7621.0 62 5757.5 63 4367.5 64 3329.0 65 2764.5 66 2201.0 67 1574.5 68 1168.0 69 972.5 70 843.0 71 641.0 72 466.5 73 314.5 74 217.5 75 179.0 76 146.0 77 131.0 78 117.0 79 88.5 80 89.0 81 84.5 82 57.5 83 36.5 84 26.5 85 24.0 86 17.5 87 13.0 88 11.0 89 6.5 90 2.0 91 2.5 92 1.5 93 1.5 94 2.5 95 1.5 96 1.0 97 2.0 98 2.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14684868869396997 2 0.015388335641583678 3 0.007694167820791839 4 0.014289168810041988 5 0.003737167227241751 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0013190001978500298 28-29 0.006595000989250148 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.2975004946250746E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.001538833564158368 66-67 0.0 68-69 0.005825584207170964 70-71 0.016267669106817033 72-73 0.0 74-75 0.008133834553408516 76-77 3.2975004946250746E-4 78-79 0.0010991668315416914 80-81 0.0013190001978500298 82-83 0.0 84-85 0.01802633603728374 86-87 0.0018685836136208755 88-89 0.018136252720437906 90-91 0.0 92-93 0.013190001978500296 94-95 0.013959418760579479 96-97 0.006045417573479302 98-99 0.003187583811470905 100-101 9.892501483875222E-4 102-103 0.0 104-105 5.495834157708457E-4 106-107 0.0014289168810041988 108-109 0.0 110-111 0.0 112-113 0.001648750247312537 114-115 0.005276000791400119 116-117 0.0 118-119 0.06858801028820155 120-121 0.0020884169799292134 122-123 0.005056167425091781 124-125 0.24137703620655543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 454890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.76896150833925 #Duplication Level Percentage of deduplicated Percentage of total 1 85.28308301414829 56.0897980407002 2 7.5076592563352404 9.875419052952788 3 2.5753005578810595 5.081245297910521 4 1.2700098442053922 3.341089142350255 5 0.8149243211086963 2.679836315360368 6 0.5181773121449516 2.044799021817361 7 0.3843653146501366 1.7695515309055876 8 0.29542691338279514 1.5543937035840423 9 0.2242481076757802 1.3273708645841678 >10 1.0834040496498474 11.85138928384759 >50 0.024705360490081287 1.0594434419814625 >100 0.016358954786799274 1.9199530354037586 >500 0.0013354248805550425 0.6560864215217784 >1k 0.001001568660416282 0.7496248470801042 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1166 0.25632570511552244 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1158 0.2545670381850557 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1090 0.23961836927608873 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 898 0.19741036294488776 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 824 0.18114269383807075 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 757 0.16641385829541208 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 509 0.11189518345094418 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.1983336630833827E-4 0.0 0.0 0.0 0.0 6 2.1983336630833827E-4 0.0 0.0 0.0 0.0 7 4.3966673261667654E-4 0.0 0.0 0.0 0.0 8 4.3966673261667654E-4 0.0 0.0 0.0 0.0 9 4.3966673261667654E-4 0.0 0.0 0.0 0.0 10-11 0.004946250741937611 0.0 0.0 2.1983336630833827E-4 0.0 12-13 0.010112334850183561 0.0 0.0 5.495834157708457E-4 0.0 14-15 0.01143133504803359 0.0 0.0 9.892501483875222E-4 0.0 16-17 0.01143133504803359 0.0 0.0 0.0014289168810041988 0.0 18-19 0.01143133504803359 0.0 0.0 0.0021983336630833827 0.0 20-21 0.011541251731187759 0.0 0.0 0.0052760007914001185 0.0 22-23 0.011651168414341929 0.0 0.0 0.020884169799292134 0.0 24-25 0.012200751830112775 0.0 0.0 0.05078150761722614 0.0 26-27 0.01275033524588362 0.0 0.0 0.13080085295346128 0.0 28-29 0.013299918661654466 0.0 0.0 0.4254874804897888 0.0 30-31 0.01406933544373365 0.0 0.0 1.03453582184704 0.0 32-33 0.01406933544373365 0.0 0.0 1.9065048693090638 0.0 34-35 0.01406933544373365 0.0 0.0 3.1184462177669325 0.0 36-37 0.014948668908967002 0.0 0.0 4.745762711864407 0.0 38-39 0.015608169007892018 0.0 0.0 6.923432038514806 0.0 40-41 0.016047835740508692 0.0 0.0 9.43524808195388 0.0 42-43 0.017147002572050388 0.0 0.0 11.38121304051529 0.0 44-45 0.017806502670975402 0.0 0.0 13.073380377673724 0.0 46-47 0.017806502670975402 0.0 0.0 15.067269010090351 0.0 48-49 0.017806502670975402 0.0 0.0 17.18899074501528 0.0 50-51 0.01791641935412957 0.0 0.0 19.31664798083053 0.0 52-53 0.018246169403592076 0.0 0.0 21.217657015981885 0.0 54-55 0.018246169403592076 0.0 0.0 22.97016861219196 0.0 56-57 0.018246169403592076 0.0 0.0 24.786321967948297 0.0 58-59 0.018356086086746246 0.0 0.0 26.51575106069599 0.0 60-61 0.018466002769900416 0.0 0.0 28.37301325595199 0.0 62-63 0.018795752819362924 0.0 0.0 30.18718811141155 0.0 64-65 0.019345336235133768 0.0 0.0 31.87957528193629 0.0 66-67 0.019345336235133768 0.0 0.0 33.66264371606322 0.0 68-69 0.019345336235133768 0.0 0.0 35.54551649849414 0.0 70-71 0.019345336235133768 0.0 0.0 37.594363472487856 0.0 72-73 0.019345336235133768 0.0 0.0 39.633537778364 0.0 74-75 0.019345336235133768 0.0 0.0 41.445514300160475 0.0 76-77 0.019345336235133768 0.0 0.0 43.257051155224346 0.0 78-79 0.019345336235133768 0.0 0.0 45.09562751434413 0.0 80-81 0.019345336235133768 0.0 0.0 46.854184528127675 0.0 82-83 0.019345336235133768 0.0 0.0 48.71870122447185 0.0 84-85 0.019345336235133768 0.0 0.0 50.73138560970784 0.0 86-87 0.019345336235133768 0.0 0.0 52.33792785068918 0.0 88-89 0.019345336235133768 0.0 0.0 53.72771439249049 0.0 90-91 0.019345336235133768 0.0 0.0 55.45362615137726 0.0 92-93 0.019345336235133768 0.0 0.0 56.93585262371123 0.0 94-95 0.019345336235133768 0.0 0.0 58.27716590824156 0.0 96-97 0.019455252918287938 0.0 0.0 59.5980346897052 0.0 98-99 0.019565169601442108 0.0 0.0 60.784475367671305 0.0 100-101 0.019565169601442108 0.0 0.0 61.947393875442415 0.0 102-103 0.019565169601442108 0.0 0.0 62.985886697883004 0.0 104-105 0.019565169601442108 0.0 0.0 64.01536635230495 0.0 106-107 0.01967508628459628 0.0 0.0 64.97405966277562 0.0 108-109 0.019785002967750445 0.0 0.0 65.8475675438018 0.0 110-111 0.019785002967750445 0.0 0.0 66.73547451032118 0.0 112-113 0.019785002967750445 0.0 0.0 67.63942931258106 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGTA 15 2.7023468E-4 118.9812 7 TTCGTAC 15 2.7023468E-4 118.9812 8 TATTATG 30 0.0042357137 59.497143 2 GTATAAA 115 2.728484E-10 46.598843 1 GTGGAAT 235 0.0 45.607376 1 GGACTGG 70 5.4518358E-5 42.49329 8 AGGGGTT 85 3.4800141E-6 41.993362 6 TGGAATC 260 0.0 41.19033 2 GTATTAG 75 8.1461134E-5 39.69531 1 CTAGGGT 90 5.150305E-6 39.66476 4 GTATTGG 250 0.0 38.1075 1 TGTAAGG 95 7.4647724E-6 37.57714 2 GTCATAG 100 1.0555414E-5 35.72578 1 GTGTAAG 100 1.0555414E-5 35.72578 1 AGTTAGA 85 1.7093448E-4 34.998314 2 GTCTTGG 140 7.996459E-8 34.02455 1 TCGGGAC 105 1.4822084E-5 33.998367 3 GCAGAGA 840 0.0 33.998367 3 TAGAGGC 35 0.0027908422 33.99463 24-25 ACGGGAT 105 1.4833247E-5 33.99463 5 >>END_MODULE