FastQCFastQC Report
Thu 26 May 2016
SRR935982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454890
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT78241.7199762579964386No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT42960.9444041416606214RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT23920.5258414122095452RNA PCR Primer, Index 33 (95% over 24bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA11570.2543472048187474No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT11160.24533403680010551No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT10700.23522170194992198No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC9730.21389786541801317No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA8400.18466002769900414No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC7990.17564685968036228No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG6980.1534436896832201RNA PCR Primer, Index 15 (95% over 24bp)
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC6910.15190485611906174No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA5050.11101584998571083No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT5000.10991668315416914RNA PCR Primer, Index 33 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT4680.10288201543230231No Hit
GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4640.10200268196706896No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4640.10200268196706896RNA PCR Primer, Index 33 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTC208.4326457E-489.371284
TATAGGT208.4326457E-489.371283
ATTAGGG508.834468E-871.4970253
GGACGTC250.002056634371.394856
GTATTAG603.0911178E-759.6596641
TAGCGTT300.00421219359.5808534
TCACGCG300.004236117559.4957129
TAGGCGG603.1504896E-759.4957129
CTTTACA1150.057.0657651
TGTGTAT859.804353E-1055.995969
CGTCAGA752.2839231E-855.60882
TATTAGG655.4229713E-754.997712
GGTATAG350.00771110451.1368561
GTACGTT350.007751511451.06933
AAGTGTA350.00779546350.9963239
ATGTGTA1004.14002E-947.596578
TCAGATG951.4691068E-743.9016844
TAACGTT355.4533913E-542.49693714-15
AGTAGGC853.4779587E-641.996977
GTCTAGG1850.041.9230081