FastQCFastQC Report
Thu 26 May 2016
SRR935981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442990
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT11870.2679518725027653No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA11580.2614054493329421No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT11090.25034425156324075No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA8410.18984627192487416No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC8400.18962053319488023No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC8010.18081672272511795No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4780.10790311293708661No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGGA250.002056327471.3972244
CATAATC150.00424067459.48425358-59
TATGGGT551.3096886E-554.0888024
ATGGGTG551.311441E-554.076595
TCTAAGG453.169407E-452.886832
TGAGGTA350.007801265550.9865078
GTAACAA1300.050.41251
TCTATGG1207.2759576E-1249.5814062
TAACAAC2450.048.6354331
ACAACAC5050.042.4046175
TCATAGG1002.2220956E-741.648382
GAAAACA4450.041.504061
GTCTAAG600.001299201539.7189371
CTATGGG1202.071829E-839.6651233
GTCTATG1551.1823431E-1038.4376831
GCAGAGA8450.037.3180773
GATTGAT801.1870428E-437.2365041
GGAGTAG401.1983186E-437.18185822-23
GTCATAG1156.577011E-736.2651181
GTATCGG1156.577011E-736.2651181