##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935981_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 442990 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.233519944016795 31.0 30.0 34.0 12.0 34.0 2 28.059940404975283 31.0 30.0 34.0 7.0 34.0 3 28.11078579651911 31.0 30.0 34.0 7.0 34.0 4 31.116546648908553 37.0 33.0 37.0 7.0 37.0 5 31.353904151335243 37.0 33.0 37.0 7.0 37.0 6 31.569735208469716 37.0 33.0 37.0 8.0 37.0 7 31.533124901239304 37.0 33.0 37.0 8.0 37.0 8 31.318345786586605 37.0 33.0 37.0 7.0 37.0 9 32.38072191245852 39.0 34.0 39.0 7.0 39.0 10-11 32.475351588071966 39.0 33.0 39.0 7.0 39.0 12-13 32.47149258448272 39.0 33.0 39.0 7.0 39.0 14-15 33.46884354048624 39.5 33.0 41.0 7.0 41.0 16-17 33.650343122869586 39.0 33.0 41.0 8.0 41.0 18-19 33.87758414411161 39.5 33.0 41.0 8.0 41.0 20-21 34.08567236280729 40.0 33.0 41.0 8.0 41.0 22-23 33.87227251179485 40.0 32.5 41.0 4.5 41.0 24-25 33.577172170929366 39.0 32.0 41.0 2.0 41.0 26-27 33.38068353687442 39.0 32.0 41.0 2.0 41.0 28-29 33.11903090363214 39.0 32.0 41.0 2.0 41.0 30-31 32.831754667148246 39.0 32.0 41.0 2.0 41.0 32-33 32.45263324228537 39.0 32.0 41.0 2.0 41.0 34-35 32.07654348856633 39.0 31.5 41.0 2.0 41.0 36-37 31.82321045621797 39.0 31.0 41.0 2.0 41.0 38-39 31.619813088331565 39.0 31.0 41.0 2.0 41.0 40-41 31.33112824217251 38.0 31.0 41.0 2.0 41.0 42-43 31.012871622384253 38.0 30.5 41.0 2.0 41.0 44-45 30.62825458813969 37.0 30.0 40.0 2.0 41.0 46-47 30.23847265175286 36.5 30.0 40.0 2.0 41.0 48-49 29.828919388699518 36.0 29.5 39.5 2.0 41.0 50-51 29.429079663197815 35.0 29.0 39.0 2.0 41.0 52-53 29.081645183864197 35.0 28.5 39.0 2.0 41.0 54-55 28.711319668615545 35.0 28.0 37.5 2.0 40.0 56-57 28.367281428474683 35.0 28.0 37.0 2.0 40.0 58-59 28.031622609991196 35.0 27.0 36.0 2.0 39.0 60-61 27.73443418587327 35.0 26.0 36.0 2.0 39.0 62-63 27.45297410776767 35.0 26.0 35.0 2.0 37.5 64-65 27.2185015463103 35.0 26.0 35.0 2.0 37.0 66-67 27.06252398474006 35.0 26.0 35.0 2.0 36.0 68-69 26.94844014537574 35.0 26.0 35.0 2.0 36.0 70-71 26.7426341452403 35.0 25.0 35.0 2.0 35.0 72-73 26.54142870042213 35.0 24.5 35.0 2.0 35.0 74-75 26.323091943384725 34.5 23.5 35.0 2.0 35.0 76-77 26.085898101537282 34.0 20.0 35.0 2.0 35.0 78-79 25.87508295898327 34.0 18.5 35.0 2.0 35.0 80-81 25.580895731280613 34.0 12.5 35.0 2.0 35.0 82-83 25.24552134359692 34.0 4.5 35.0 2.0 35.0 84-85 24.931650827332447 34.0 2.0 35.0 2.0 35.0 86-87 24.640595724508454 34.0 2.0 35.0 2.0 35.0 88-89 24.26547100386013 33.0 2.0 35.0 2.0 35.0 90-91 23.849458227048014 33.0 2.0 35.0 2.0 35.0 92-93 23.405580261405447 33.0 2.0 35.0 2.0 35.0 94-95 22.989164540960292 32.0 2.0 35.0 2.0 35.0 96-97 22.569758911036367 31.5 2.0 35.0 2.0 35.0 98-99 22.122502765299444 31.0 2.0 35.0 2.0 35.0 100-101 21.741006568997044 30.5 2.0 35.0 2.0 35.0 102-103 20.942125104404163 29.0 2.0 34.0 2.0 35.0 104-105 21.209357999051896 29.5 2.0 35.0 2.0 35.0 106-107 21.086201720129125 29.0 2.0 35.0 2.0 35.0 108-109 20.799968396577803 29.0 2.0 35.0 2.0 35.0 110-111 20.39107429061604 29.0 2.0 35.0 2.0 35.0 112-113 19.938779656425652 28.0 2.0 35.0 2.0 35.0 114-115 19.375945280931848 26.0 2.0 35.0 2.0 35.0 116-117 18.71813584956771 25.0 2.0 34.0 2.0 35.0 118-119 17.85885234429671 21.0 2.0 34.0 2.0 35.0 120-121 16.953126481410415 8.5 2.0 34.0 2.0 35.0 122-123 16.036110295943477 2.0 2.0 34.0 2.0 35.0 124-125 13.836450032732117 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3928432282882284 1101 2 0.26162513514171337 1101 3 0.20830911682791609 1101 4 0.002776667338149963 1101 5 0.03423936809745953 1101 6 0.430722411609068 1101 7 0.19759467823278953 1101 8 0.4261163821675531 1101 9 0.25374105396958413 1101 10-11 0.5576003790129391 1101 12-13 0.2935798039707578 1101 14-15 0.1543134116821605 1101 16-17 0.28874054346696454 1101 18-19 0.5074405958405634 1101 20-21 0.6556911153078886 1101 22-23 0.7771847379310373 1101 24-25 0.9001106081796095 1101 26-27 1.0883906072426797 1101 28-29 0.8812161670117575 1101 30-31 0.7573817365655771 1101 32-33 0.5432603394514146 1101 34-35 0.4093011886628517 1101 36-37 0.4258834506051876 1101 38-39 0.384631557891435 1101 40-41 0.4224582692257144 1101 42-43 0.32848421225396507 1101 44-45 0.39077474759355013 1101 46-47 0.3389384930545205 1101 48-49 0.3375373525841745 1101 50-51 0.23319637056972198 1101 52-53 0.03711682055234178 1101 54-55 -0.019544689569411133 1101 56-57 -0.031170120599266937 1101 58-59 -0.016893327796847046 1101 60-61 0.17753197274485188 1101 62-63 0.2285134219507512 1101 64-65 0.09204851815556125 1101 66-67 -0.09904114180053725 1101 68-69 -0.06255198231264814 1101 70-71 -0.30434225737695186 1101 72-73 -0.026242793262081676 1101 74-75 0.023230179782267157 1101 76-77 0.1776881921730613 1101 78-79 0.011494611969691704 1101 80-81 -0.27971083703664945 1101 82-83 -0.013731140193844027 1101 84-85 -0.20106275969914122 1101 86-87 -0.19949013565758378 1101 88-89 -0.18318695384626693 1101 90-91 0.032437445934277775 1101 92-93 -0.1495922895128423 1101 94-95 -0.17699570477536142 1101 96-97 -0.21161677815683788 1101 98-99 -0.1449883213344556 1101 100-101 0.08404877935532795 1101 102-103 0.13200339649626613 1101 104-105 -0.16384216048325229 1101 106-107 -0.3341463758747878 1101 108-109 -0.13925022447686075 1101 110-111 -0.35974186025043053 1101 112-113 -0.40378179885281185 1101 114-115 -0.4972001392387355 1101 116-117 -0.4611799734974298 1101 118-119 -0.693628038972296 1101 120-121 -0.9896005763033884 1101 122-123 -0.8156805742129283 1101 124-125 -0.5188880153449116 1102 1 0.5858851940629037 1102 2 0.5957524371028242 1102 3 0.6032422620793447 1102 4 0.5806981630674564 1102 5 0.6870788270432051 1102 6 0.6924899830214173 1102 7 0.27226458851533053 1102 8 0.26264352154939274 1102 9 0.802810706037981 1102 10-11 0.8764470225279979 1102 12-13 0.6492159850314678 1102 14-15 0.5403674573358543 1102 16-17 0.6326181188840252 1102 18-19 0.43650642251432714 1102 20-21 0.35004208331205433 1102 22-23 0.6942947120498459 1102 24-25 0.7583842195314716 1102 26-27 0.9586096110860112 1102 28-29 0.9453483104879581 1102 30-31 0.9346618809742253 1102 32-33 1.0831415729021003 1102 34-35 1.1340655383756513 1102 36-37 1.0992634856314574 1102 38-39 1.1526213727781958 1102 40-41 1.042892451517723 1102 42-43 1.0957463055816454 1102 44-45 0.9576459334061163 1102 46-47 0.9375395342791251 1102 48-49 1.0594580593258343 1102 50-51 1.053441674001224 1102 52-53 1.0641142443881364 1102 54-55 1.0183909720744317 1102 56-57 1.2286817649018893 1102 58-59 1.1068746261549656 1102 60-61 1.2330360581467872 1102 62-63 1.3191020545514789 1102 64-65 1.2259104333632962 1102 66-67 1.1192768202204633 1102 68-69 1.2270528066032718 1102 70-71 1.3376770130485625 1102 72-73 0.9832579329651807 1102 74-75 1.0272677642846375 1102 76-77 1.2151546452551933 1102 78-79 1.3194448579306126 1102 80-81 1.490938822562999 1102 82-83 1.2138499551839992 1102 84-85 1.0956309636650552 1102 86-87 0.9432015674200684 1102 88-89 1.1527036159582202 1102 90-91 1.202543481092043 1102 92-93 1.2238947370198545 1102 94-95 1.0894564807801892 1102 96-97 1.009032283724899 1102 98-99 1.1003195930484324 1102 100-101 1.189620705740392 1102 102-103 1.1142481858903608 1102 104-105 1.0001402546557436 1102 106-107 0.6407165972936539 1102 108-109 0.9485948829031621 1102 110-111 0.9011261457028397 1102 112-113 0.5919519895430909 1102 114-115 0.8651289881528825 1102 116-117 0.7162376460312103 1102 118-119 0.6261659874348844 1102 120-121 0.3086068676755005 1102 122-123 -0.171449747509282 1102 124-125 -0.24231048834300495 1103 1 0.18889481243088113 1103 2 0.24213779236190192 1103 3 0.27504718690940066 1103 4 0.11302546964235916 1103 5 0.09938244027762977 1103 6 -0.07897928615185634 1103 7 0.04803250660406988 1103 8 0.1918427430539218 1103 9 0.24015028237849378 1103 10-11 0.20946820550264533 1103 12-13 0.06726523333203716 1103 14-15 0.11935372083964779 1103 16-17 0.10222999940189226 1103 18-19 -0.03865223824780628 1103 20-21 0.10536039205884862 1103 22-23 0.016082713065273424 1103 24-25 -0.01352647914000471 1103 26-27 0.4011998009147959 1103 28-29 0.29408325534453184 1103 30-31 0.4434566233091459 1103 32-33 0.41754063645102235 1103 34-35 0.16585269320034968 1103 36-37 0.21248662612732616 1103 38-39 0.19569812227753758 1103 40-41 0.3254919494445545 1103 42-43 0.14610734696036687 1103 44-45 0.056101635321329724 1103 46-47 0.03144837133099543 1103 48-49 0.11385119953963851 1103 50-51 0.09259773977844787 1103 52-53 0.33451590292197153 1103 54-55 0.3258360401927831 1103 56-57 0.2432418867533528 1103 58-59 0.22244030465889963 1103 60-61 0.24065741600085033 1103 62-63 0.3066277928528933 1103 64-65 0.29624298632073476 1103 66-67 0.4420351199412238 1103 68-69 0.556851413209202 1103 70-71 0.40062655187461615 1103 72-73 0.6256351647932341 1103 74-75 0.8225629438181024 1103 76-77 0.49537211627315614 1103 78-79 0.4340952401267586 1103 80-81 0.43912374344416705 1103 82-83 0.5848171511899807 1103 84-85 0.8123615647425382 1103 86-87 0.6466244089285418 1103 88-89 0.6138691026589242 1103 90-91 0.4476101891280351 1103 92-93 0.8454331117695268 1103 94-95 0.9525132037655517 1103 96-97 0.7651558839843986 1103 98-99 1.083828746011509 1103 100-101 0.9307060863851895 1103 102-103 0.6099918465114627 1103 104-105 0.6178862846109006 1103 106-107 0.65353074560268 1103 108-109 0.25978293469003333 1103 110-111 0.14555115910085803 1103 112-113 0.23919340775875853 1103 114-115 -0.31163038340946514 1103 116-117 0.24541712308533548 1103 118-119 0.22472746319151682 1103 120-121 0.11050797618535668 1103 122-123 0.24483237248056255 1103 124-125 -0.09188739297730919 1104 1 0.4117169341294087 1104 2 0.4846885000811483 1104 3 0.6058910111137976 1104 4 0.5178567976606132 1104 5 0.7191322512429643 1104 6 0.278082650889651 1104 7 0.2072620411145003 1104 8 0.25454388256813587 1104 9 0.567518521901988 1104 10-11 0.4283756514785253 1104 12-13 0.3627018702395617 1104 14-15 0.6127766411267217 1104 16-17 0.6270645111618407 1104 18-19 0.02868124542888495 1104 20-21 0.04255710749866637 1104 22-23 0.11561974396083485 1104 24-25 0.1707166526005679 1104 26-27 0.4874075665914077 1104 28-29 0.4479614506936187 1104 30-31 0.47477933532643846 1104 32-33 0.47678210999280424 1104 34-35 0.662540275281188 1104 36-37 0.657048397348948 1104 38-39 0.4511575760005222 1104 40-41 0.5507483069787469 1104 42-43 0.546944405713667 1104 44-45 0.38186075873111847 1104 46-47 0.4814821985864768 1104 48-49 0.6393423580236473 1104 50-51 0.47867682922832344 1104 52-53 0.6562356540594969 1104 54-55 0.6833954368737345 1104 56-57 0.6536567017646924 1104 58-59 0.8348409785508935 1104 60-61 0.8819001365081256 1104 62-63 0.8507624120641211 1104 64-65 0.680281827969381 1104 66-67 0.6156816980821844 1104 68-69 0.6552476033316417 1104 70-71 0.7554877274712375 1104 72-73 0.5488247433454276 1104 74-75 0.7290657362248787 1104 76-77 0.8979854375790204 1104 78-79 0.8346470886867792 1104 80-81 0.6143754580217227 1104 82-83 0.7088318494641292 1104 84-85 0.7397987853360384 1104 86-87 0.6016978339713219 1104 88-89 0.6694890700255698 1104 90-91 0.7372497630372692 1104 92-93 0.9265336251478722 1104 94-95 0.8902949317413551 1104 96-97 0.6110282955360731 1104 98-99 0.7171863995938814 1104 100-101 0.48929091320290397 1104 102-103 0.7490139071882709 1104 104-105 0.668995951246611 1104 106-107 0.5640370931876824 1104 108-109 0.4955615585176858 1104 110-111 0.5857551838809805 1104 112-113 0.660423658611073 1104 114-115 0.8142919067620582 1104 116-117 0.4734128250340497 1104 118-119 0.19799995168361662 1104 120-121 0.6177271104729307 1104 122-123 0.45066525257289314 1104 124-125 0.5066732904659066 1105 1 0.5177088555306391 1105 2 0.6511288013529111 1105 3 0.6015518510764046 1105 4 0.6020118079793448 1105 5 0.9254187416386053 1105 6 0.9885583709961878 1105 7 0.81380958666686 1105 8 0.8626965645570941 1105 9 0.7638214164392352 1105 10-11 0.938156400424603 1105 12-13 0.877994763542091 1105 14-15 0.8631073697881035 1105 16-17 0.9932208267563496 1105 18-19 1.1324065730707389 1105 20-21 1.0351487854836066 1105 22-23 1.133505549968632 1105 24-25 1.195358142731628 1105 26-27 1.3907025060019222 1105 28-29 1.3227519892080721 1105 30-31 1.3759046947182583 1105 32-33 1.3093981296072172 1105 34-35 1.6204980478457003 1105 36-37 1.5223404087083772 1105 38-39 1.343163643769028 1105 40-41 1.4449504130588622 1105 42-43 1.5052637749479665 1105 44-45 1.4939469069328375 1105 46-47 1.4005286417099683 1105 48-49 1.3976634911441366 1105 50-51 1.4244373287608916 1105 52-53 1.3106021543328978 1105 54-55 1.5352642742963454 1105 56-57 1.25658828061923 1105 58-59 1.1498622333795332 1105 60-61 1.0775503931535653 1105 62-63 1.1554737520884615 1105 64-65 0.9519379407169879 1105 66-67 0.8926559277048867 1105 68-69 0.9324511381595748 1105 70-71 0.7866820548132338 1105 72-73 0.9210047302926689 1105 74-75 0.8105308200976715 1105 76-77 0.7318538163912223 1105 78-79 0.8760454976614334 1105 80-81 0.92820608966937 1105 82-83 0.8164024490090434 1105 84-85 0.9147851152180344 1105 86-87 0.9319326072237573 1105 88-89 0.9960285017014385 1105 90-91 1.1465683738005055 1105 92-93 1.071913125262526 1105 94-95 1.1768823411217006 1105 96-97 1.0634562848822 1105 98-99 1.3705563041676356 1105 100-101 1.2609823036743926 1105 102-103 1.3951430589354032 1105 104-105 1.1446543217685452 1105 106-107 1.0796474860051362 1105 108-109 1.0202471458035944 1105 110-111 0.9109364231874224 1105 112-113 0.6872700064847095 1105 114-115 0.6287487180085982 1105 116-117 0.7577424600859963 1105 118-119 0.800181878905672 1105 120-121 0.930490590973168 1105 122-123 0.4302923670314662 1105 124-125 0.5302917266044069 1106 1 -0.37761383229862133 1106 2 -0.4302269594507031 1106 3 -0.36235584508370167 1106 4 -1.2481822573768575 1106 5 -0.49912691428303546 1106 6 -0.27213448566511644 1106 7 -0.24061780014439904 1106 8 -0.34795343399825285 1106 9 -0.07804421135978146 1106 10-11 -0.09921201555906123 1106 12-13 -0.1570718350652669 1106 14-15 -0.2364390084611685 1106 16-17 -0.23539105216227796 1106 18-19 -0.3951117018182657 1106 20-21 -0.03433311298601893 1106 22-23 0.02013382090891014 1106 24-25 0.1547440679370098 1106 26-27 -0.010503540361888497 1106 28-29 0.19462069893830858 1106 30-31 0.029445063201656296 1106 32-33 -0.041248594396208205 1106 34-35 -0.24523208092123738 1106 36-37 -0.4528837536323458 1106 38-39 -0.38204486813974015 1106 40-41 -0.42355104604294525 1106 42-43 -0.3820615421538669 1106 44-45 -0.5257786567234923 1106 46-47 -0.49693093202041894 1106 48-49 -0.3452143584383549 1106 50-51 -0.3749566383587499 1106 52-53 -0.2327748236409235 1106 54-55 -0.09064345075686475 1106 56-57 -0.2166374419491781 1106 58-59 -0.1729495954577125 1106 60-61 -0.14787120540562881 1106 62-63 -0.13105917160484637 1106 64-65 -0.07406705779020228 1106 66-67 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2216 72-73 -2.1474374884693788 2216 74-75 -2.322607896196697 2216 76-77 -2.3695224193133733 2216 78-79 -2.6688158243853906 2216 80-81 -2.4984610598122785 2216 82-83 -2.6803364197980564 2216 84-85 -2.4694319470374033 2216 86-87 -2.564033047590513 2216 88-89 -2.4131199191740578 2216 90-91 -2.399608264060568 2216 92-93 -2.054357192905446 2216 94-95 -2.2486462880316616 2216 96-97 -2.384085512339766 2216 98-99 -2.169088486010203 2216 100-101 -1.9069403919668524 2216 102-103 -1.864709029274895 2216 104-105 -2.1645609831823656 2216 106-107 -1.911869525767738 2216 108-109 -2.2225409829120366 2216 110-111 -2.3182948998605326 2216 112-113 -2.3332254894905304 2216 114-115 -2.458219750622984 2216 116-117 -2.1797709859562318 2216 118-119 -1.9653798514748217 2216 120-121 -1.6561381321161086 2216 122-123 -1.4554636679783002 2216 124-125 -1.2705971499774336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35786.0 3 3555.0 4 1966.0 5 3969.0 6 11374.0 7 10144.0 8 6208.0 9 6067.0 10 4663.0 11 3074.0 12 2280.0 13 1815.0 14 1928.0 15 2126.0 16 1796.0 17 1838.0 18 2084.0 19 2426.0 20 3146.0 21 3902.0 22 4797.0 23 5640.0 24 6443.0 25 6756.0 26 7549.0 27 7998.0 28 9167.0 29 11142.0 30 14635.0 31 19540.0 32 25191.0 33 31194.0 34 38845.0 35 51249.0 36 72162.0 37 19414.0 38 1120.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.21460688635547 18.618614545397 10.98444002866489 14.182338539582643 2 19.66951813947608 26.05248245204085 33.77832564587101 20.499673762612066 3 31.434273381554828 24.988034928977147 23.922692095828932 19.654999593639097 4 16.561872788886806 23.52748609834896 27.77620994597377 32.13443116679046 5 15.95545592660567 35.57701641394474 24.29584380227416 24.17168385717543 6 30.947876927244405 37.29519853721303 9.039255965145939 22.717668570396622 7 27.94500076751515 42.360515408995276 10.913162433293905 18.78132139019567 8 35.78395851815734 28.987627232278907 9.670894762623902 25.557519486939857 9 36.38434276168762 18.69703605047518 13.990157791372265 30.928463396464934 10-11 30.284882277252308 28.601209959592765 14.049414207995667 27.06449355515926 12-13 32.58820740874512 30.287929750107228 16.33772771394388 20.786135127203774 14-15 36.05781168875144 23.952798031558274 20.12065735118174 19.868732928508546 16-17 30.103162599607213 24.97663604144563 21.296078918260005 23.62412244068715 18-19 28.620172012912253 25.589855301474074 21.029707216867198 24.76026546874647 20-21 38.19770197972866 23.314183164405517 17.21810876092011 21.27000609494571 22-23 34.124359466353646 26.262443847490914 16.450484209575837 23.162712476579607 24-25 27.129732048127497 28.67288200636583 18.279532269351453 25.917853676155218 26-27 24.058100896638283 28.69276368660099 20.999891644920403 26.24924377184032 28-29 25.396089002067924 27.67204869106638 22.549643756941997 24.382218549923696 30-31 26.375200343122867 28.67355922255581 19.642091243594663 25.309149190726654 32-33 23.481455563331 29.07740581051491 20.83963520621233 26.601503419941757 34-35 22.51743831689203 29.708909907672858 21.2333235513217 26.540328224113413 36-37 23.097248244881374 30.888394771891015 19.370301812682005 26.64405517054561 38-39 23.58811711325312 28.219711505903067 20.862773426036703 27.329397954807106 40-41 22.098580103388336 28.584618162938213 22.161335470326645 27.1554662633468 42-43 21.795074380911533 28.6741235693808 23.897604912074765 25.6331971376329 44-45 22.12295988622768 27.983927402424435 24.423914761055553 25.469197950292333 46-47 19.97076683446579 28.134833743425357 23.895686132869816 27.998713289239035 48-49 19.176392243617236 28.599742657847806 24.967606492245874 27.25625860628908 50-51 20.070656222488093 28.426149574482494 23.77299713311813 27.730197069911284 52-53 20.225964468723898 27.18368360459604 23.201878146233547 29.38847378044651 54-55 19.150319420302942 26.599923248831804 23.02252872525339 31.227228605611867 56-57 19.595520430529877 25.756847235484404 24.113126962520514 30.534505371465205 58-59 21.25454299194113 24.013747488656627 23.612384026727465 31.119325492674776 60-61 20.670556897446897 23.767127926138286 23.490146504435767 32.07216867197905 62-63 20.211291451274295 23.17715975529922 23.97920946296756 32.632339330458926 64-65 20.089438896212954 22.28635797229697 24.51984721795821 33.10435591353186 66-67 20.645387029052575 23.206844398293416 24.159461838867692 31.988306733786313 68-69 19.96453448406253 22.671178680958224 24.97734022175542 32.38694661322383 70-71 19.224395244137582 21.86513616884847 26.722487390100834 32.187981196913114 72-73 19.781394367135114 20.807039444501505 27.680791531176247 31.730774657187133 74-75 19.948821101870163 20.028400111975003 28.204607229611973 31.81817155654286 76-77 20.049120803089014 19.513780800673604 28.926644988199495 31.510453408037886 78-79 20.75972798329524 19.8747142978075 29.93097999379215 29.43457772510511 80-81 21.827880407730675 20.821420872099388 29.30064775947908 28.050050960690854 82-83 21.448678299735885 20.650804758572427 29.258561141335022 28.641955800356666 84-85 21.320786027876963 20.478403650398896 29.30095357865754 28.899856743066604 86-87 21.911835251226076 20.43625240559622 28.89345395646506 28.758458386712643 88-89 22.565026511829007 20.14176518997416 28.463894354566506 28.829313943630332 90-91 23.427391137497462 20.34255852276575 27.732228718481228 28.49782162125556 92-93 23.814463829138752 21.225243123952296 27.157975538033448 27.802317508875507 94-95 23.116440675670184 21.934221042222614 26.615124464371988 28.334213817735215 96-97 22.658711211795925 22.67552963937454 25.803644293642975 28.862114855186565 98-99 22.147162400108357 23.924217797643234 25.383313016389003 28.54530678585941 100-101 21.819528046439128 25.80495881947274 25.2564118481631 27.11910128592503 102-103 21.17813043183819 27.630759159348973 25.275175511862567 25.915934896950272 104-105 20.151967656081755 29.59001239308425 24.976692423513903 25.28132752732009 106-107 19.33338976833432 30.951550551652137 24.616383080786704 25.098676599226838 108-109 19.15122237522292 32.543172532111335 24.447165850244925 23.858439242420822 110-111 18.977064945032616 34.82392379060475 23.362942730084203 22.836068534278425 112-113 18.694863066268162 36.11136193305462 22.76066168835273 22.433113312324487 114-115 18.308771739719347 36.814284553286015 22.844652435390365 22.032291271604272 116-117 17.973430551479716 37.537754802591486 22.642384703943655 21.846429941985146 118-119 17.91338449270222 38.21489645762409 22.47051408614563 21.401204963528052 120-121 18.269354492815783 38.015056943237354 22.054019887806582 21.661568676140273 122-123 18.766888879357246 38.20120595346401 22.005038727315004 21.02686643986374 124-125 18.69149574051657 40.30987317713342 21.06832143544026 19.930309646909752 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 6.5 2 8.5 3 8.5 4 9.0 5 11.0 6 13.5 7 16.5 8 19.0 9 24.0 10 22.0 11 20.5 12 32.5 13 42.5 14 47.0 15 56.5 16 77.0 17 96.0 18 126.0 19 186.0 20 275.5 21 387.5 22 555.5 23 778.5 24 1067.5 25 1404.0 26 1756.0 27 2146.5 28 2534.5 29 2894.5 30 3205.5 31 3548.5 32 3783.0 33 3806.5 34 3884.5 35 3911.5 36 3775.0 37 3603.0 38 3377.5 39 3118.0 40 2823.0 41 2577.0 42 2395.5 43 2310.0 44 2351.0 45 2588.5 46 3132.0 47 4009.5 48 5616.5 49 9591.0 50 17195.5 51 27595.0 52 38022.0 53 44248.0 54 43891.0 55 39515.5 56 33824.0 57 26656.0 58 19825.5 59 14390.5 60 10290.5 61 7651.5 62 6011.5 63 4984.5 64 3978.0 65 3088.0 66 2473.5 67 2088.0 68 1789.0 69 1529.5 70 1231.5 71 985.0 72 774.0 73 610.0 74 521.5 75 442.0 76 373.0 77 280.5 78 190.5 79 114.5 80 82.0 81 75.0 82 53.5 83 39.0 84 32.0 85 22.5 86 19.5 87 16.5 88 10.5 89 9.0 90 8.0 91 5.5 92 4.5 93 2.0 94 1.0 95 1.5 96 1.5 97 1.5 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1437955710061175 2 0.013770062529628207 3 0.007675116819792773 4 0.012867107609652588 5 0.0029346034899207655 6 0.0 7 4.514774599878101E-4 8 2.2573872999390505E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.514774599878101E-4 28-29 0.007675116819792773 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.2573872999390505E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0013544323799634303 66-67 0.0 68-69 0.005530598884850674 70-71 0.018962053319488026 72-73 1.1286936499695252E-4 74-75 0.007675116819792773 76-77 1.1286936499695252E-4 78-79 5.643468249847627E-4 80-81 3.386080949908576E-4 82-83 0.0 84-85 0.01794622903451545 86-87 0.0028217341249238133 88-89 0.016817535384545928 90-91 0.0 92-93 0.01297997697464954 94-95 0.011738413959683062 96-97 0.005304860154856769 98-99 0.0022573872999390506 100-101 3.386080949908576E-4 102-103 0.0 104-105 2.2573872999390505E-4 106-107 5.643468249847627E-4 108-109 0.0 110-111 0.0 112-113 4.514774599878101E-4 114-115 0.004966252059865911 116-117 0.0 118-119 0.07246213232804352 120-121 0.0011286936499695253 122-123 0.0047405133298720065 124-125 0.23363958554369174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 442990.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.54544611118767 #Duplication Level Percentage of deduplicated Percentage of total 1 85.56534915740805 56.939843313392544 2 7.42503586680929 9.882046482967864 3 2.484208986301248 4.959383856805134 4 1.313821011966288 3.4971522140619467 5 0.7582430732835415 2.522881178618561 6 0.5245238872528017 2.0942805643927196 7 0.3824191916559168 1.7813778997155933 8 0.28200720804834056 1.501303637291787 9 0.1968293758277109 1.1788288760017476 >10 1.0214937757556322 11.242346622730645 >50 0.030486484702743524 1.3734547611583279 >100 0.01354954868565767 1.6890360222148368 >500 0.001016216151424325 0.5594805027542007 >1k 0.001016216151424325 0.7785840678940407 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1187 0.2679518725027653 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1158 0.2614054493329421 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1109 0.25034425156324075 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 841 0.18984627192487416 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 840 0.18962053319488023 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 801 0.18081672272511795 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 478 0.10790311293708661 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.2573872999390505E-4 0.0 0.0 0.0 0.0 3 2.2573872999390505E-4 0.0 0.0 0.0 0.0 4 2.2573872999390505E-4 0.0 0.0 0.0 0.0 5 9.029549199756202E-4 0.0 0.0 0.0 0.0 6 9.029549199756202E-4 0.0 0.0 0.0 0.0 7 0.0011286936499695253 0.0 0.0 0.0 0.0 8 0.0011286936499695253 0.0 0.0 0.0 0.0 9 0.0011286936499695253 0.0 0.0 0.0 0.0 10-11 0.002144517934942098 0.0 0.0 2.2573872999390505E-4 0.0 12-13 0.004063297139890291 0.0 0.0 2.2573872999390505E-4 0.0 14-15 0.005079121424862864 0.0 0.0 4.514774599878101E-4 0.0 16-17 0.006094945709835436 0.0 0.0 9.029549199756203E-4 0.0 18-19 0.006997900629811057 0.0 0.0 0.0018059098399512404 0.0 20-21 0.007787986184789724 0.0 0.0 0.0050791214248628635 0.0 22-23 0.00846520237477144 0.0 0.0 0.022573872999390506 0.0 24-25 0.009481026659744013 0.0 0.0 0.053612948373552446 0.0 26-27 0.010609720309713537 0.0 0.0 0.1269780356215716 0.0 28-29 0.011625544594686111 0.0 0.0 0.40960292557394073 0.0 30-31 0.012867107609652588 0.0 0.0 1.0030700467279172 0.0 32-33 0.013431454434637351 0.0 0.0 1.8353687442154452 0.0 34-35 0.013544323799634304 0.0 0.0 3.0445382514277974 0.0 36-37 0.014108670624619065 0.0 0.0 4.7231314476624755 0.0 38-39 0.014447278719609923 0.0 0.0 6.90850809273347 0.0 40-41 0.014673017449603828 0.0 0.0 9.348630894602586 0.0 42-43 0.016027449829567258 0.0 0.0 11.274859477640579 0.0 44-45 0.01738188220953069 0.0 0.0 12.98370166369444 0.0 46-47 0.017946229034515454 0.0 0.0 14.98261811779047 0.0 48-49 0.018059098399512405 0.0 0.0 17.150951488746927 0.0 50-51 0.018510575859500215 0.0 0.0 19.19004943678187 0.0 52-53 0.018849183954491075 0.0 0.0 21.02778843766225 0.0 54-55 0.018962053319488026 0.0 0.0 22.828732025553624 0.0 56-57 0.019074922684484977 0.0 0.0 24.66331068421409 0.0 58-59 0.01918779204948193 0.0 0.0 26.433215196731304 0.0 60-61 0.01918779204948193 0.0 0.0 28.29036772839116 0.0 62-63 0.019300661414478885 0.0 0.0 30.07223639359805 0.0 64-65 0.019413530779475836 0.0 0.0 31.788979435201696 0.0 66-67 0.019526400144472787 0.0 0.0 33.62784712973205 0.0 68-69 0.02110657125443012 0.0 0.0 35.565024041174745 0.0 70-71 0.022573872999390506 0.0 0.0 37.647238086638524 0.0 72-73 0.022573872999390506 0.0 0.0 39.65134653152441 0.0 74-75 0.022573872999390506 0.0 0.0 41.45240298878079 0.0 76-77 0.022573872999390506 0.0 0.0 43.31384455631053 0.0 78-79 0.022573872999390506 1.1286936499695252E-4 0.0 45.16129032258064 0.0 80-81 0.022912481094381362 2.2573872999390505E-4 0.0 46.933565091762794 0.0 82-83 0.023025350459378317 3.386080949908576E-4 0.0 48.747601525993815 0.0 84-85 0.023025350459378317 6.772161899817152E-4 0.0 50.71130273821079 0.0 86-87 0.023025350459378317 9.029549199756202E-4 0.0 52.316530847197456 0.0 88-89 0.023025350459378317 0.0011286936499695253 0.0 53.7111447210998 0.0 90-91 0.023025350459378317 0.0011286936499695253 0.0 55.41310187588884 0.0 92-93 0.02313821982437527 0.0011286936499695253 0.0 56.927357276687964 0.0 94-95 0.023928305379353934 0.0011286936499695253 0.0 58.26192464841193 0.0 96-97 0.02415404410934784 0.0011286936499695253 0.0 59.571999367931554 0.0 98-99 0.02415404410934784 0.0011286936499695253 0.0 60.722025327885504 0.0 100-101 0.02415404410934784 0.0011286936499695253 0.0 61.834917266755454 0.0 102-103 0.02415404410934784 0.0011286936499695253 0.0 62.87625002821734 0.0 104-105 0.024379782839341745 0.0011286936499695253 0.0 63.84365335560622 0.0 106-107 0.024379782839341745 0.0011286936499695253 0.0 64.7444637576469 0.0 108-109 0.024379782839341745 0.0011286936499695253 0.0 65.6040768414637 0.0 110-111 0.024379782839341745 0.0011286936499695253 0.0 66.45330594370077 0.0 112-113 0.024379782839341745 0.0011286936499695253 0.0 67.2777037856385 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAGGA 25 0.0020563274 71.397224 4 CATAATC 15 0.004240674 59.484253 58-59 TATGGGT 55 1.3096886E-5 54.088802 4 ATGGGTG 55 1.311441E-5 54.07659 5 TCTAAGG 45 3.169407E-4 52.88683 2 TGAGGTA 35 0.0078012655 50.986507 8 GTAACAA 130 0.0 50.4125 1 TCTATGG 120 7.2759576E-12 49.581406 2 TAACAAC 245 0.0 48.635433 1 ACAACAC 505 0.0 42.404617 5 TCATAGG 100 2.2220956E-7 41.64838 2 GAAAACA 445 0.0 41.50406 1 GTCTAAG 60 0.0012992015 39.718937 1 CTATGGG 120 2.071829E-8 39.665123 3 GTCTATG 155 1.1823431E-10 38.437683 1 GCAGAGA 845 0.0 37.318077 3 GATTGAT 80 1.1870428E-4 37.236504 1 GGAGTAG 40 1.1983186E-4 37.181858 22-23 GTCATAG 115 6.577011E-7 36.265118 1 GTATCGG 115 6.577011E-7 36.265118 1 >>END_MODULE