FastQCFastQC Report
Thu 26 May 2016
SRR935981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442990
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT73871.6675319984649766No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT42140.9512630081943159TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT24300.5485451138851893RNA PCR Primer, Index 10 (95% over 23bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA12330.27833585408248496No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT10730.24221765728346012No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT10550.23815436014356983No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT9290.2097112801643378TruSeq Adapter, Index 10 (96% over 26bp)
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC8940.20181042461455112No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA8870.20023025350459378No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG7930.1790108128851667TruSeq Adapter, Index 10 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC7390.16682092146549585No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC7320.1652407503555385No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG6660.15034199417594077TruSeq Adapter, Index 10 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT5640.12731664371656246No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA5220.11783561705681843No Hit
GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.10880606785706223No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT4500.10158242849725727No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACA304.22065E-579.486862
CTATACA701.6916601E-1068.247391
TCATATT300.004202532659.6151472
GTTACAC401.753938E-459.6151433
TAAGTAG150.00423034659.520893118-119
TTAAGTT300.004236521759.4940159
GTAGTGT805.7298166E-1059.4940158
CTTTACA901.5752448E-953.0813031
TATCACA505.278467E-447.6921163
ATCGGTT255.3345814E-447.59521516-17
CGGAGAT653.442016E-545.935741
GTCTAGG951.4429679E-744.0016021
TTCTAGG558.441971E-443.3564722
TATACGG705.3847383E-542.582252
TCAATTT355.4541903E-542.49572814-15
TAGATGG1404.0017767E-1142.4957289
TGTGTAT1404.0017767E-1142.4957289
TAGTGTT1151.4239049E-841.3871429
AGTAGAT1455.820766E-1141.0303577
GTAATAG1202.0057996E-839.8109741
Produced by FastQC (version 0.11.5)