##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935981_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 442990 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27439219846949 33.0 31.0 34.0 30.0 34.0 2 32.36124291744735 33.0 31.0 34.0 30.0 34.0 3 32.35486128355042 34.0 31.0 34.0 30.0 34.0 4 35.87157949389377 37.0 35.0 37.0 35.0 37.0 5 35.86322038872209 37.0 35.0 37.0 35.0 37.0 6 35.95852728052552 37.0 35.0 37.0 35.0 37.0 7 35.93782026682318 37.0 35.0 37.0 35.0 37.0 8 35.96262218108761 37.0 35.0 37.0 35.0 37.0 9 37.57683469152803 39.0 37.0 39.0 35.0 39.0 10-11 37.53995011174067 39.0 37.0 39.0 35.0 39.0 12-13 37.5091570915822 39.0 37.0 39.0 34.5 39.0 14-15 38.574628095442336 40.0 38.0 41.0 34.0 41.0 16-17 38.529754622000496 40.0 38.0 41.0 34.0 41.0 18-19 38.6536118196799 40.0 38.0 41.0 34.0 41.0 20-21 38.782785164450665 40.0 38.0 41.0 35.0 41.0 22-23 38.61446533781801 40.0 38.0 41.0 34.0 41.0 24-25 38.285495157904236 40.0 38.0 41.0 33.5 41.0 26-27 38.10596966071469 40.0 37.0 41.0 33.0 41.0 28-29 37.96071356012551 40.0 37.0 41.0 33.0 41.0 30-31 37.6886385697194 40.0 36.5 41.0 33.0 41.0 32-33 37.20107903112937 39.5 35.0 41.0 32.5 41.0 34-35 36.87468227273753 39.0 35.0 41.0 32.0 41.0 36-37 36.62017088421861 39.0 35.0 41.0 31.0 41.0 38-39 36.04896385922933 39.0 35.0 41.0 30.0 41.0 40-41 35.74881600036119 39.0 35.0 41.0 30.0 41.0 42-43 35.30816271247658 38.5 35.0 41.0 28.5 41.0 44-45 34.675446398338565 38.0 35.0 40.5 26.0 41.0 46-47 33.885319081694846 37.0 34.5 40.0 21.5 41.0 48-49 32.86526106684124 36.5 33.5 39.5 5.0 41.0 50-51 31.868980112417887 36.0 33.0 39.0 2.0 41.0 52-53 31.279218492516762 35.0 32.5 39.0 2.0 41.0 54-55 30.79977200388271 35.0 32.0 37.5 2.0 40.5 56-57 30.45155533984966 35.0 32.0 37.0 2.0 40.0 58-59 30.154311609742884 35.0 32.0 36.5 2.0 39.0 60-61 29.769055734892437 35.0 31.0 36.0 2.0 39.0 62-63 29.433626041219895 35.0 31.0 35.0 2.0 37.5 64-65 29.127532224203705 35.0 31.0 35.0 2.0 37.0 66-67 28.7056039639721 35.0 30.5 35.0 2.0 36.0 68-69 28.228677848258425 35.0 30.0 35.0 2.0 36.0 70-71 27.741611548793426 35.0 29.0 35.0 2.0 35.0 72-73 27.43371069324364 35.0 27.5 35.0 2.0 35.0 74-75 27.301757376013 35.0 27.0 35.0 2.0 35.0 76-77 27.170261179710604 35.0 27.0 35.0 2.0 35.0 78-79 27.20969660714689 35.0 27.0 35.0 2.0 35.0 80-81 27.168377390008803 35.0 27.0 35.0 2.0 35.0 82-83 27.050598207634483 35.0 26.0 35.0 2.0 35.0 84-85 26.995998781010858 35.0 25.5 35.0 2.0 35.0 86-87 27.00145488611481 35.0 27.0 35.0 2.0 35.0 88-89 26.93910584889049 34.5 25.5 35.0 2.0 35.0 90-91 26.825673265762205 34.0 25.0 35.0 2.0 35.0 92-93 26.74369850335222 34.0 25.0 35.0 2.0 35.0 94-95 26.71381408158198 34.0 25.0 35.0 2.0 35.0 96-97 26.6814916815278 34.0 25.0 35.0 2.0 35.0 98-99 26.60264340052823 34.0 25.0 35.0 2.0 35.0 100-101 26.506310526196977 34.0 24.5 35.0 2.0 35.0 102-103 25.756575769204723 33.0 21.5 35.0 2.0 35.0 104-105 26.159485541434343 34.0 23.5 35.0 2.0 35.0 106-107 26.003056502404117 34.0 20.0 35.0 2.0 35.0 108-109 25.684070746517982 34.0 16.5 35.0 2.0 35.0 110-111 25.23180432966884 34.0 4.5 35.0 2.0 35.0 112-113 24.63109551005666 34.0 2.0 35.0 2.0 35.0 114-115 23.91698458204474 33.0 2.0 35.0 2.0 35.0 116-117 23.206850041761665 33.0 2.0 35.0 2.0 35.0 118-119 22.60074493780898 33.0 2.0 35.0 2.0 35.0 120-121 21.92923542292151 33.0 2.0 35.0 2.0 35.0 122-123 21.192092372288315 32.5 2.0 35.0 2.0 35.0 124-125 19.043540486241227 27.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03554342747131045 1101 2 0.06688089065468006 1101 3 0.15927654301965788 1101 4 0.27353745968062526 1101 5 0.29450627728242296 1101 6 0.2530688383502948 1101 7 0.3517699445869482 1101 8 0.27271144670175573 1101 9 0.36738261364215674 1101 10-11 0.3307031731699226 1101 12-13 0.22818431909266224 1101 14-15 0.31821371726081793 1101 16-17 0.4081827384224326 1101 18-19 0.37272123108101596 1101 20-21 0.255592876567988 1101 22-23 0.2794294614409125 1101 24-25 0.3524201097345596 1101 26-27 0.16721670522907317 1101 28-29 0.513570131241444 1101 30-31 0.33239115653841367 1101 32-33 0.2691643425434975 1101 34-35 0.48761082264520894 1101 36-37 0.6375970520259671 1101 38-39 0.515703082051175 1101 40-41 0.5105572896451847 1101 42-43 0.576854554108273 1101 44-45 0.7933909674075679 1101 46-47 0.531106964594791 1101 48-49 0.5748539855416794 1101 50-51 0.1929054208411003 1101 52-53 -0.02006161062001155 1101 54-55 -0.0072434332299486925 1101 56-57 -0.14488037448012392 1101 58-59 -0.028970131830448054 1101 60-61 -0.3036173451075115 1101 62-63 -0.202935548534942 1101 64-65 0.043134787285712406 1101 66-67 -0.05141935604047276 1101 68-69 0.1842848652084399 1101 70-71 0.0984271199620359 1101 72-73 -0.07602674043015867 1101 74-75 0.08053383580168827 1101 76-77 0.17301489529209135 1101 78-79 0.16090894939047118 1101 80-81 0.17613688096865587 1101 82-83 0.06635851944053783 1101 84-85 -0.005179751056889614 1101 86-87 -0.12178221026147895 1101 88-89 -0.16937549539611751 1101 90-91 -0.15097789882904422 1101 92-93 -0.12164511600638406 1101 94-95 0.04108653496055226 1101 96-97 -0.14780085209121907 1101 98-99 -0.15787880188737446 1101 100-101 -0.2305709206710489 1101 102-103 -0.17736549287217684 1101 104-105 -0.013292205262672496 1101 106-107 -0.37552529369281373 1101 108-109 -0.15077257629103613 1101 110-111 -0.1917349280393843 1101 112-113 -0.13628475299219645 1101 114-115 -0.2785409766706586 1101 116-117 -0.22454411151003129 1101 118-119 -0.4389384660519333 1101 120-121 -0.16208946432755766 1101 122-123 -0.2883563500415427 1101 124-125 -0.10997689010449463 1102 1 -0.034273013357605464 1102 2 0.07763383590969397 1102 3 -0.04736350527594624 1102 4 0.0032016019853031707 1102 5 0.10909119207773443 1102 6 -0.001950497824196873 1102 7 0.029842065230326398 1102 8 0.06278096129504007 1102 9 -0.07558613168816919 1102 10-11 0.07804930563617063 1102 12-13 0.028177445336659446 1102 14-15 0.05974554872827298 1102 16-17 -0.008897349800733423 1102 18-19 -0.18042485715410095 1102 20-21 -0.03976046437628611 1102 22-23 0.16206007774785292 1102 24-25 0.18368733148193428 1102 26-27 0.20699632663454537 1102 28-29 -0.04442643785773015 1102 30-31 0.15426822987242161 1102 32-33 0.14957593109839706 1102 34-35 0.35775511174941244 1102 36-37 0.4943763685953044 1102 38-39 0.36382893753493306 1102 40-41 0.2823724991751959 1102 42-43 0.4314178315151338 1102 44-45 0.5491634281524398 1102 46-47 0.29370088102187 1102 48-49 0.47425955507718953 1102 50-51 0.5320072301332246 1102 52-53 0.6726277015859878 1102 54-55 0.6904771695087959 1102 56-57 0.6174625004234926 1102 58-59 0.6000114166175763 1102 60-61 0.6414374357392134 1102 62-63 0.8721154069171426 1102 64-65 0.7849012230340229 1102 66-67 0.6912675652938738 1102 68-69 0.8635075928343667 1102 70-71 0.6397368992248111 1102 72-73 0.6579813168073088 1102 74-75 0.717699636773574 1102 76-77 0.7373158940787228 1102 78-79 0.660275368402683 1102 80-81 0.6151024105766112 1102 82-83 0.5977566414108573 1102 84-85 0.5966489668979555 1102 86-87 0.7509008856782202 1102 88-89 0.7697887754179149 1102 90-91 0.814318599141707 1102 92-93 0.8067796582056737 1102 94-95 0.5256295497301622 1102 96-97 0.6498492199337882 1102 98-99 0.5916041721178722 1102 100-101 0.5971114878930557 1102 102-103 0.5891673846016268 1102 104-105 0.5106538686272124 1102 106-107 0.7696245243020812 1102 108-109 0.8508487335479984 1102 110-111 0.9764494440280629 1102 112-113 0.5678478686583439 1102 114-115 0.5467146971666317 1102 116-117 0.6960206721459699 1102 118-119 0.6624987466698933 1102 120-121 0.5760279035618403 1102 122-123 0.4739939963359845 1102 124-125 0.6008217805708718 1103 1 -0.0010431033358884179 1103 2 -0.017720576974966207 1103 3 -0.08426024180035796 1103 4 0.12563975954601858 1103 5 0.12423518553386259 1103 6 0.24348148254418334 1103 7 0.19385357342427767 1103 8 0.19484312810743631 1103 9 0.20862676989984408 1103 10-11 0.25595103468589286 1103 12-13 0.09860448959243939 1103 14-15 0.10474597182363254 1103 16-17 0.05667820092840259 1103 18-19 0.04303719119160121 1103 20-21 0.10940885838906667 1103 22-23 0.23772504263093452 1103 24-25 0.13938078478630445 1103 26-27 0.22650807458253297 1103 28-29 0.4960468648246845 1103 30-31 0.36545769197718414 1103 32-33 0.3523401541542768 1103 34-35 0.42945708337381205 1103 36-37 0.42288312401797157 1103 38-39 0.7417151530879238 1103 40-41 0.5121161033835904 1103 42-43 0.6725370597891853 1103 44-45 0.6224830343916921 1103 46-47 0.6699412556023034 1103 48-49 0.4198960315389826 1103 50-51 0.6787747815393104 1103 52-53 0.892516004409444 1103 54-55 0.6999615572898037 1103 56-57 0.8791878833247999 1103 58-59 0.8396610936547795 1103 60-61 0.9008202806324555 1103 62-63 0.9863553866085617 1103 64-65 0.9575650314564434 1103 66-67 1.0284477757133033 1103 68-69 0.9939084051774678 1103 70-71 1.0246591907093112 1103 72-73 0.8986703981262387 1103 74-75 0.8435521739354535 1103 76-77 0.8183739145565383 1103 78-79 0.9455234433187734 1103 80-81 0.9983351412341008 1103 82-83 1.0020693935051774 1103 84-85 1.046482549387452 1103 86-87 0.8611200490776518 1103 88-89 0.7462618524494786 1103 90-91 0.8737127265780273 1103 92-93 0.9113504984730696 1103 94-95 0.7884733147147891 1103 96-97 1.027502733035643 1103 98-99 0.7899667930526313 1103 100-101 0.8177204631560784 1103 102-103 1.0924858625865639 1103 104-105 1.1241294560246153 1103 106-107 1.137418505065348 1103 108-109 1.0880486884178246 1103 110-111 1.1108992790208703 1103 112-113 1.065769060094894 1103 114-115 0.8686098823413708 1103 116-117 1.0925724449155538 1103 118-119 1.2059689748593279 1103 120-121 1.216395432402841 1103 122-123 0.627188366346985 1103 124-125 0.5764641803677826 1104 1 0.09007027766513431 1104 2 0.21901184280137187 1104 3 0.3020549751583701 1104 4 0.15736043927486776 1104 5 0.2588756217064727 1104 6 0.1745295968405074 1104 7 0.21032678356448997 1104 8 0.1219354433509281 1104 9 0.12734584449226105 1104 10-11 0.22477822383994805 1104 12-13 0.2942507228280462 1104 14-15 0.4202958968730499 1104 16-17 0.4227076770702354 1104 18-19 0.31617039098465227 1104 20-21 0.33306671228185536 1104 22-23 0.47643536193369584 1104 24-25 0.33357310931570794 1104 26-27 0.5119106189227693 1104 28-29 0.40974226033826966 1104 30-31 0.6108008064724899 1104 32-33 0.5873453940624742 1104 34-35 0.6089630727878728 1104 36-37 0.6895798593561864 1104 38-39 0.726841773148152 1104 40-41 0.4830830322667552 1104 42-43 0.4426921345407848 1104 44-45 0.5299237040386942 1104 46-47 0.6250889679512426 1104 48-49 1.0137182460966443 1104 50-51 0.8731740492787168 1104 52-53 0.9399842664844442 1104 54-55 0.883922131955174 1104 56-57 0.8611241288974085 1104 58-59 0.8548528477637944 1104 60-61 0.8361054244977808 1104 62-63 0.8954542550537035 1104 64-65 0.9740183432110818 1104 66-67 0.9302452632104838 1104 68-69 0.8007401842257948 1104 70-71 0.8133860920000551 1104 72-73 0.9105643264655185 1104 74-75 0.9453509879916737 1104 76-77 0.7886788904794884 1104 78-79 0.9492656663664079 1104 80-81 0.7523977282895373 1104 82-83 1.0294150815250198 1104 84-85 1.1607297934610479 1104 86-87 1.0047452696180912 1104 88-89 1.1434349551384138 1104 90-91 1.1306181296529942 1104 92-93 1.151277272414145 1104 94-95 1.056655796176127 1104 96-97 0.7876184089837999 1104 98-99 0.8953844601659036 1104 100-101 0.9584219475756441 1104 102-103 1.0039924356865981 1104 104-105 0.8052936442368086 1104 106-107 0.7838653166806964 1104 108-109 0.775594952164294 1104 110-111 0.8383789495036069 1104 112-113 0.8693149524048316 1104 114-115 0.6881461173015033 1104 116-117 0.8362852183525362 1104 118-119 0.6633081627398845 1104 120-121 0.878564621742612 1104 122-123 0.843408203710851 1104 124-125 0.5403127934503082 1105 1 -0.08002126802833942 1105 2 -0.07672131649518832 1105 3 0.1442047996516962 1105 4 0.23800531606742226 1105 5 0.15530022439111235 1105 6 -0.06426531611170816 1105 7 0.07475449068883222 1105 8 -0.0015189513715228031 1105 9 0.06730315844636436 1105 10-11 0.06438805221382893 1105 12-13 0.13117062475597407 1105 14-15 0.056935340896117737 1105 16-17 0.24193580047171537 1105 18-19 0.10689411349008537 1105 20-21 0.13669326229685197 1105 22-23 0.15992362327106946 1105 24-25 0.11307851562689564 1105 26-27 0.18584743651930324 1105 28-29 0.2260687909505137 1105 30-31 0.3522150904228951 1105 32-33 0.504098785133678 1105 34-35 0.5541414898764003 1105 36-37 0.45191383356816317 1105 38-39 0.4962489309983198 1105 40-41 0.42648874373024626 1105 42-43 0.49617316394476774 1105 44-45 0.6580435777444364 1105 46-47 0.9845954325941406 1105 48-49 0.9954620239880967 1105 50-51 0.7166732467104993 1105 52-53 0.8807706069497598 1105 54-55 0.8968425983266854 1105 56-57 0.8619090582537687 1105 58-59 0.8829074576803677 1105 60-61 0.9559262136604652 1105 62-63 0.9956694491693803 1105 64-65 1.1526647369457557 1105 66-67 1.116467548148659 1105 68-69 1.074407127234629 1105 70-71 0.9807083363688456 1105 72-73 1.0427587772795555 1105 74-75 1.1952407451045417 1105 76-77 1.0593472269584225 1105 78-79 1.1933522379008146 1105 80-81 1.2210799289139551 1105 82-83 1.027201404351473 1105 84-85 1.2029131317920587 1105 86-87 1.3878348926756132 1105 88-89 1.250626319151607 1105 90-91 1.0842191033181692 1105 92-93 1.425058868025598 1105 94-95 1.2953184176637862 1105 96-97 1.2050420768087555 1105 98-99 1.246935798073583 1105 100-101 1.3472882979187197 1105 102-103 1.1999160946921208 1105 104-105 1.2516097679205096 1105 106-107 1.081388820464234 1105 108-109 1.0049266156853633 1105 110-111 1.1982911079711407 1105 112-113 1.3295481381596872 1105 114-115 1.439103025561785 1105 116-117 1.705572431941512 1105 118-119 1.6386843114707688 1105 120-121 2.0188553101873943 1105 122-123 2.200409949958182 1105 124-125 2.344847744855244 1106 1 0.11755700820901183 1106 2 0.05281128937396318 1106 3 0.09226462764140564 1106 4 0.1323552693568999 1106 5 0.19124324718777075 1106 6 0.17241977793534602 1106 7 0.07859727367271319 1106 8 0.053840238080567815 1106 9 0.09172264780888639 1106 10-11 -0.11821998392181854 1106 12-13 -0.05402695592801621 1106 14-15 0.049200415873727366 1106 16-17 0.15234189306448798 1106 18-19 0.22398582983683468 1106 20-21 0.08026141631635397 1106 22-23 0.1001293421796845 1106 24-25 0.07889556448513702 1106 26-27 -0.06410440830546094 1106 28-29 0.02948371520242432 1106 30-31 0.12571601981778002 1106 32-33 0.058388929695524894 1106 34-35 0.2687221546104439 1106 36-37 0.3121253792529828 1106 38-39 0.3180182002916965 1106 40-41 0.1351463219101774 1106 42-43 0.0314251599767843 1106 44-45 -0.06524956206929744 1106 46-47 -0.16712587414469482 1106 48-49 -0.03560769507461714 1106 50-51 0.20188358851594046 1106 52-53 0.1313894009515515 1106 54-55 0.10436251049164724 1106 56-57 0.14738617973000956 1106 58-59 0.12739369011576684 1106 60-61 0.18738165567681975 1106 62-63 0.30548660396900473 1106 64-65 0.32449323710467226 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-1.054107322914298 2216 30-31 -0.99764826583705 2216 32-33 -0.9124803713175424 2216 34-35 -1.290439302465586 2216 36-37 -1.2307372040935292 2216 38-39 -1.7007789877779942 2216 40-41 -1.5134050511742032 2216 42-43 -1.3850181394213763 2216 44-45 -1.2131651708630287 2216 46-47 -1.2202647392169936 2216 48-49 -1.6237822711623835 2216 50-51 -1.4533209382090533 2216 52-53 -1.3745124894847223 2216 54-55 -1.7152968613607484 2216 56-57 -1.5428301258031176 2216 58-59 -1.5937544731393487 2216 60-61 -1.5107499673014289 2216 62-63 -1.8799453411575655 2216 64-65 -1.930038274328684 2216 66-67 -1.9961852574678964 2216 68-69 -2.001823678207259 2216 70-71 -1.965485129014386 2216 72-73 -1.710321830811715 2216 74-75 -1.7305072808567026 2216 76-77 -1.8932950135302988 2216 78-79 -1.9598176199598747 2216 80-81 -1.954545085730249 2216 82-83 -1.8451490596834255 2216 84-85 -1.8316844667714243 2216 86-87 -2.1144442780470527 2216 88-89 -2.2317324734417276 2216 90-91 -2.2207488498374133 2216 92-93 -2.3387913542467977 2216 94-95 -2.3998084171218963 2216 96-97 -2.337650837952392 2216 98-99 -2.6501713591615044 2216 100-101 -2.3243172820024256 2216 102-103 -2.4956601840398065 2216 104-105 -2.545686752190509 2216 106-107 -2.4074932995479514 2216 108-109 -2.437275105834054 2216 110-111 -2.332865368814332 2216 112-113 -1.9270396237585672 2216 114-115 -2.1200542350880127 2216 116-117 -2.1935753286134982 2216 118-119 -1.7651794154206755 2216 120-121 -1.7916593350820484 2216 122-123 -1.7773755907271465 2216 124-125 -1.9149383113060772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 24.0 5 115.0 6 327.0 7 866.0 8 1367.0 9 2093.0 10 3008.0 11 5484.0 12 8026.0 13 9026.0 14 12129.0 15 15492.0 16 7030.0 17 2897.0 18 2947.0 19 4219.0 20 5572.0 21 6762.0 22 3659.0 23 2146.0 24 2738.0 25 3636.0 26 4597.0 27 5329.0 28 6795.0 29 8486.0 30 11114.0 31 15026.0 32 20096.0 33 26835.0 34 36302.0 35 55931.0 36 115132.0 37 35922.0 38 1855.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.96589819221512 20.530075460089396 12.184904362932267 15.319121984763214 2 19.557051464380365 21.364332055640585 37.48244865526224 21.596167824716808 3 28.89704056524978 23.11745186121583 29.103591503194203 18.88191607034019 4 15.505100495812485 22.98725892982242 27.834198858320562 33.67344171604453 5 14.797795155393672 34.332709334911016 28.082864581804383 22.786630927890922 6 30.40062666344606 36.2376715931383 9.562486878669741 23.799214864745892 7 26.139416239644238 41.24878665432628 12.16370572699158 20.4480913790379 8 35.320887604686334 27.34553827400167 12.570938395900585 24.762635725411407 9 32.91925325628118 14.320187814623356 17.94171425991557 34.818844669179896 10-11 29.441635251360076 24.936115939411724 18.668480101130953 26.953768708097247 12-13 33.2927379850561 25.87552766428136 19.970202487640805 20.86153186302174 14-15 34.782726472380865 19.459807219124585 24.848416442809093 20.909049865685457 16-17 30.188604708909907 21.165714801688527 26.152960563443873 22.492719925957697 18-19 28.56249576739881 20.996523623558094 26.751281067292716 23.68969954175038 20-21 33.89038127271496 17.892503216776902 26.959412176347097 21.257703334161043 22-23 29.99593670286011 20.9149190726653 27.002302535045935 22.086841689428656 24-25 25.504413192171384 21.84744576627012 28.802343168017337 23.845797873541162 26-27 23.110453960586018 21.297546220004968 31.209846723402336 24.382153096006682 28-29 23.67073748843089 18.909907672859433 33.110228221856026 24.309126616853654 30-31 26.08546468317569 20.0667057947132 30.65407797015734 23.193751551953767 32-33 24.039368834510938 18.84365335560622 32.717442831666624 24.399534978216213 34-35 20.903519266800604 19.498634280683536 34.168829996162444 25.429016456353416 36-37 23.233030090972708 20.771574979119166 30.807580306553195 25.18781462335493 38-39 23.570735231043592 17.15343461477686 32.568003792410664 26.707826361768888 40-41 22.04293550644484 17.488656628817807 33.63665094020181 26.831756924535544 42-43 23.241608162712478 17.49046253865776 34.771778143976164 24.496151154653603 44-45 22.734034628321183 17.811350143344093 34.0553962843405 25.399218943994224 46-47 21.62915641436601 17.410889636334907 32.28571751055328 28.674236438745798 48-49 20.87823652904129 18.40888056163796 32.38549402921059 28.32738888011016 50-51 21.17564730580826 19.176956590442224 30.911871599810382 28.735524503939143 52-53 20.95408474231924 19.24005056547552 30.16625657464051 29.63960811756473 54-55 19.19411273392176 20.03634393552902 27.950179462290347 32.819363868258876 56-57 19.89740174721777 20.79369737465857 26.8999300209937 32.40897085712996 58-59 19.787241246980745 20.749001106119778 26.8000406329714 32.66371701392808 60-61 20.201923293979547 22.546445743696246 23.938350752838662 33.31328020948554 62-63 18.558093862163933 23.52186279599991 24.113072529853945 33.80697081198221 64-65 18.186189304498974 22.9295243684959 24.3147700851035 34.569516241901624 66-67 19.20697984153141 23.90482855143457 23.76487053883835 33.12332106819567 68-69 19.457211223729658 23.34003024898982 23.99185083184722 33.210907695433306 70-71 19.599426623625817 22.510440416262217 24.826519785999686 33.063613174112284 72-73 20.358811711325313 21.384681369782612 25.138152102756273 33.118354816135806 74-75 20.863450642226685 20.79753493306847 25.114110928011918 33.224903496692924 76-77 21.002054222442943 20.512991263911147 25.735005304860152 32.74994920878575 78-79 20.87552766428136 20.62710219192307 26.47734711844511 32.02002302535046 80-81 20.77270367276914 21.291338404930134 25.98004469626854 31.95591322603219 82-83 20.118738571976795 21.125420438384616 26.14517257725908 32.61066841237951 84-85 20.40068624573918 20.247296778708325 26.320232962369356 33.03178401318314 86-87 20.74155172802998 20.173028736540328 26.046863360346734 33.03855617508296 88-89 20.887717555701034 20.109370414682047 25.701483103456056 33.30142892616086 90-91 20.979141741348563 19.600893925370777 25.530824623580667 33.8891397097 92-93 20.987832682453327 19.53813855843247 25.426081852863497 34.0479469062507 94-95 20.7962933700535 19.152915415697873 25.491997562021716 34.55879365222691 96-97 21.03219034289713 19.48667012799386 25.227657509198853 34.25348201991016 98-99 21.488408316214812 19.90112643626267 24.96264024018601 33.64782500733651 100-101 21.90512201178356 20.79042416307366 24.397390460281272 32.907063364861514 102-103 21.66403304815007 21.82306598343078 24.012731664371657 32.500169304047496 104-105 21.462448362265512 23.580103388338337 23.64556762003657 31.311880629359578 106-107 21.329826858394092 25.49041739091176 23.097135375516377 30.082620375177772 108-109 20.953859003589244 26.506580283979318 22.90965936025644 29.629901352174993 110-111 20.722080255720297 27.693663879508062 22.84506889639355 28.73918696837809 112-113 20.828395287467636 28.740668504184075 22.68442331525162 27.74651289309667 114-115 20.49166172860408 29.29416744264793 22.774344648551033 27.43982618019696 116-117 19.9967943363599 29.692685217375598 23.02422759308841 27.286292853176093 118-119 19.93744645644673 30.160018330377973 23.41897668181782 26.483558531357478 120-121 20.298833942641853 30.536120012144785 23.340447889730772 25.82459815548259 122-123 19.81698886063319 30.605209959399836 23.487483026828613 26.09031815313836 124-125 19.015872621618783 31.83691809889821 23.921155103252765 25.226054176230246 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.5 4 3.0 5 4.5 6 6.5 7 8.5 8 12.5 9 21.0 10 26.0 11 22.0 12 20.5 13 25.0 14 27.0 15 26.5 16 35.5 17 53.5 18 66.0 19 84.0 20 117.0 21 166.0 22 242.5 23 347.0 24 484.5 25 720.5 26 1006.5 27 1298.5 28 1675.5 29 2090.5 30 2567.0 31 3100.5 32 3660.0 33 4194.5 34 4525.0 35 4676.0 36 4662.5 37 4505.0 38 4181.5 39 3803.0 40 3415.0 41 3122.0 42 3082.0 43 3220.5 44 3462.5 45 3780.5 46 4320.0 47 4951.0 48 5743.5 49 7446.0 50 10644.0 51 15801.0 52 23543.0 53 33191.0 54 41674.0 55 45713.0 56 44375.0 57 38167.0 58 29730.0 59 20945.0 60 13248.0 61 8399.0 62 5957.5 63 4645.5 64 3468.5 65 2553.5 66 2031.5 67 1689.5 68 1402.0 69 1162.5 70 896.5 71 685.0 72 506.0 73 393.5 74 333.5 75 291.0 76 259.5 77 158.5 78 61.5 79 17.5 80 14.0 81 12.5 82 7.0 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20361633445450236 2 9.029549199756202E-4 3 0.0 4 0.10925754531705004 5 0.03318359330910404 6 0.0020316485699451454 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0013544323799634303 112-113 2.2573872999390505E-4 114-115 5.643468249847627E-4 116-117 0.005417729519853721 118-119 0.002144517934942098 120-121 3.386080949908576E-4 122-123 0.002595995394929908 124-125 0.027201516964265558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 442990.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.977100578484695 #Duplication Level Percentage of deduplicated Percentage of total 1 82.14043330318114 46.801237298756654 2 8.824552920494902 10.05594878622398 3 3.1156149806831865 5.325561243546587 4 1.5828408798393332 3.607427360413716 5 0.9820583384298818 2.797741836132947 6 0.7041365958753999 2.4071796986510696 7 0.513011718100639 2.046094418211294 8 0.3720581542511668 1.6959035900651283 9 0.26608149830192657 1.36444970617405 >10 1.4140860717803678 13.816568887754826 >50 0.056618381798626806 2.1595825989439197 >100 0.022568166166908626 2.4815009489829807 >500 0.003563394657932941 1.5173250348054117 >1k 0.0019796636988516337 2.257037908597702 >5k 3.9593273977032675E-4 1.6664406827397524 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 7387 1.6675319984649766 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 4214 0.9512630081943159 TruSeq Adapter, Index 10 (95% over 21bp) ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 2430 0.5485451138851893 RNA PCR Primer, Index 10 (95% over 23bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1233 0.27833585408248496 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1073 0.24221765728346012 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1055 0.23815436014356983 No Hit CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 929 0.2097112801643378 TruSeq Adapter, Index 10 (96% over 26bp) ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 894 0.20181042461455112 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 887 0.20023025350459378 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 793 0.1790108128851667 TruSeq Adapter, Index 10 (96% over 28bp) TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 739 0.16682092146549585 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 732 0.1652407503555385 No Hit CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG 666 0.15034199417594077 TruSeq Adapter, Index 10 (95% over 24bp) CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT 564 0.12731664371656246 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 522 0.11783561705681843 No Hit GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 482 0.10880606785706223 No Hit TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT 450 0.10158242849725727 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.2573872999390505E-4 0.0 0.0 0.0 0.0 6 2.2573872999390505E-4 0.0 0.0 0.0 0.0 7 2.2573872999390505E-4 0.0 0.0 0.0 0.0 8 2.2573872999390505E-4 0.0 0.0 0.0 0.0 9 2.2573872999390505E-4 0.0 0.0 0.0 0.0 10-11 0.0010158242849725727 0.0 0.0 0.0 0.0 12-13 0.0011286936499695253 0.0 0.0 0.0 0.0 14-15 0.0013544323799634303 0.0 0.0 0.0 0.0 16-17 0.0015801711099573353 0.0 0.0 4.514774599878101E-4 0.0 18-19 0.002144517934942098 0.0 0.0 0.0010158242849725727 0.0 20-21 0.0022573872999390506 0.0 0.0 0.004176166504887243 0.0 22-23 0.0024831260299329554 0.0 0.0 0.02200952617440574 0.0 24-25 0.0024831260299329554 0.0 0.0 0.05304860154856769 0.0 26-27 0.0027088647599268607 0.0 0.0 0.1255107338766112 0.0 28-29 0.0027088647599268607 0.0 0.0 0.40960292557394073 0.0 30-31 0.0032732115849116233 0.0 0.0 1.0047630872028714 0.0 32-33 0.003386080949908576 0.0 0.0 1.835594482945439 0.0 34-35 0.003386080949908576 0.0 0.0 3.04927876475767 0.0 36-37 0.0034989503149055285 0.0 0.0 4.731935258132237 0.0 38-39 0.004063297139890291 0.0 0.0 6.926792929862977 0.0 40-41 0.004289035869884196 0.0 0.0 9.372784938711934 0.0 42-43 0.004289035869884196 0.0 0.0 11.31402514729452 0.0 44-45 0.004289035869884196 0.0 0.0 13.024898981918328 0.0 46-47 0.004401905234881149 0.0 0.0 15.030474728549176 0.0 48-49 0.0047405133298720065 0.0 0.0 17.206934693785414 0.0 50-51 0.005417729519853721 0.0 0.0 19.259125488160002 0.0 52-53 0.005869206979841532 0.0 0.0 21.105442560780155 0.0 54-55 0.005869206979841532 0.0 0.0 22.909546490891444 0.0 56-57 0.006320684439829341 0.0 0.0 24.75766947335154 0.0 58-59 0.006546423169823247 0.0 0.0 26.537732228718482 0.0 60-61 0.006546423169823247 0.0 0.0 28.417571502742724 0.0 62-63 0.006546423169823247 0.0 0.0 30.238718706968555 0.0 64-65 0.006546423169823247 0.0 0.0 31.983114742996456 0.0 66-67 0.006546423169823247 0.0 0.0 33.84026727465631 0.0 68-69 0.006772161899817152 0.0 0.0 35.81706133321294 0.0 70-71 0.007449378089798867 0.0 0.0 37.93652226912572 0.0 72-73 0.007449378089798867 0.0 0.0 39.958689812411116 0.0 74-75 0.007675116819792772 0.0 0.0 41.78209440393688 0.0 76-77 0.007675116819792772 0.0 0.0 43.66193367796113 0.0 78-79 0.007675116819792772 0.0 0.0 45.53116323167566 0.0 80-81 0.007675116819792772 0.0 0.0 47.3258990044922 0.0 82-83 0.008013724914783629 0.0 0.0 49.16928147362243 0.0 84-85 0.008352333009774487 0.0 0.0 51.166956364703495 0.0 86-87 0.008352333009774487 0.0 0.0 52.79667712589449 0.0 88-89 0.008803810469762297 0.0 0.0 54.212171832321275 0.0 90-91 0.008803810469762297 0.0 0.0 55.93500981963476 0.0 92-93 0.00891667983475925 0.0 0.0 57.47736969231811 0.0 94-95 0.009706765389737918 0.0 0.0 58.83214068037653 0.0 96-97 0.01027111221472268 0.0 0.0 60.1632091017856 0.0 98-99 0.010383981579719632 0.0 0.0 61.333438678074 0.0 100-101 0.010496850944716585 0.0 0.0 62.46619562518341 0.0 102-103 0.010609720309713537 0.0 0.0 63.52163705726991 0.0 104-105 0.010609720309713537 0.0 0.0 64.51567755479807 0.0 106-107 0.010609720309713537 0.0 0.0 65.43793313618818 0.0 108-109 0.010609720309713537 0.0 0.0 66.31684688141945 0.0 110-111 0.010609720309713537 0.0 0.0 67.18357073523104 0.0 112-113 0.010609720309713537 0.0 0.0 68.03550870222804 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTACA 30 4.22065E-5 79.48686 2 CTATACA 70 1.6916601E-10 68.24739 1 TCATATT 30 0.0042025326 59.615147 2 GTTACAC 40 1.753938E-4 59.615143 3 TAAGTAG 15 0.004230346 59.520893 118-119 TTAAGTT 30 0.0042365217 59.494015 9 GTAGTGT 80 5.7298166E-10 59.494015 8 CTTTACA 90 1.5752448E-9 53.081303 1 TATCACA 50 5.278467E-4 47.692116 3 ATCGGTT 25 5.3345814E-4 47.595215 16-17 CGGAGAT 65 3.442016E-5 45.93574 1 GTCTAGG 95 1.4429679E-7 44.001602 1 TTCTAGG 55 8.441971E-4 43.356472 2 TATACGG 70 5.3847383E-5 42.58225 2 TCAATTT 35 5.4541903E-5 42.495728 14-15 TAGATGG 140 4.0017767E-11 42.495728 9 TGTGTAT 140 4.0017767E-11 42.495728 9 TAGTGTT 115 1.4239049E-8 41.387142 9 AGTAGAT 145 5.820766E-11 41.030357 7 GTAATAG 120 2.0057996E-8 39.810974 1 >>END_MODULE