FastQCFastQC Report
Thu 26 May 2016
SRR935980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591927
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT168841.0606013969233514No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG168421.0579630849907062No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC161801.0163782635761565No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT153970.9671925911175575No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT127860.8031775326381172No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA93590.5879038423244282No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT83180.5225113965652948No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG58000.36433831450814014No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC55410.3480687242568284No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG52600.33041716108841673No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG52590.3303543441376395No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC48470.3044737604174061No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG45740.2873247328552126No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA39470.24793850471786708No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC35920.2256384871919378No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT34770.21841453785255227No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG31130.19554916776962764No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA30960.19448127960641412No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA30470.19140324901832811No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC30110.18914183879034654No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC28480.17890267581365224No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT28310.17783478765043875No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT27590.17331196719447564No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA27350.17180436037582125No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC27320.17161590952348946No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG26900.1689775975908443No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG26120.1640778754302176No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT23950.15044659711155095No Hit
GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG20040.12588516935764016No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT19710.12381220998199038No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC19250.12092263024623617No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT18940.11897530477214092No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA18660.1172164301503775No Hit
GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG18240.11457811821773234No Hit
GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA17710.11124881982653727No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA17060.107165718026015No Hit
GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA16980.1066631824197969No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG16920.10628628071513328No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG16550.10396205353637447No Hit
GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA16490.10358515183171088No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG16290.10232881281616556No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG16190.10170064330839292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT32650.085.81
AGTAGTA27700.081.755422
GAGTACG7700.081.450951
GAGTCGA5250.079.640941
GAGTCCG8650.078.720231
GAGTAAG14950.078.309151
AGTCGAC1800.076.149672
TCGTAGG15800.075.43722
GAGTCAG71350.075.092421
AGTACGG5750.071.514472
AGTCGTA4950.071.03292
GAGTTAG15650.070.9898451
GTATACG1950.070.45161
GAGTCGT10200.070.2714161
GAGTTCG5700.070.209771
AGTTGCG1200.069.5279542
GAGTCGG9750.069.226351
GAGTAGG17000.069.217351
GAGTTAC9850.069.129951
GATTGTG122250.068.911219
Produced by FastQC (version 0.11.5)