FastQCFastQC Report
Thu 26 May 2016
SRR935979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439810
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC184611.2821830658211848No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT181491.2605135399809697No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT163031.1323021787596974No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG161011.1182725498503274No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT124890.8674061160847611No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA110790.7694765281530203No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT81030.5627825893694307No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC60940.4232502899688153No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC55900.3882456713038526No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG53330.3703960939290601No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG48590.3374750835179642No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG47560.33032136184635474No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA37300.25906195956410916No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA36680.25475583583945105No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC35730.24815774303553942No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC34390.2388509595016009No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA33790.23468374299386724No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA32760.2275300213222578No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG32530.22593258832762655No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC32310.22440460894145756No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT26930.1870385675887791No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG25340.1759954438432849No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG25340.1759954438432849No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT25130.1745369180655781No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT24160.167799918044742No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT24100.16738319639396865No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC21890.15203394892381633No Hit
GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA21660.1504365159291851No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA21320.14807509324146936No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG20700.14376896951681126No Hit
GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA20340.14126863961217107No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT19760.13724033032136185No Hit
GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA19690.13675415506212624No Hit
GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG18280.12696119626895216No Hit
GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG17880.12418305193046306No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA17310.12022419624811607No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA17060.11848785603656038No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA16340.11348719622727999No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG16320.11334828901035554No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT15760.1094588869364708No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA15710.10911161889415963No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC15020.10431931991026595No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG14460.1004299178363812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTA24800.096.683382
GAGTAGT31200.094.720611
GAGTCCG9000.094.007671
AGTCCGT3100.092.125112
GAGTCGT11200.088.309511
GAGTAAG13150.086.994721
AGTACGG5000.086.86632
AGTCGTA6150.086.102022
GAGTCAG73400.085.5582661
GAGTACG8400.084.408291
GAGTCGG12250.083.172061
AGTAAGG7000.082.446492
GAGTTAG16500.081.970831
GAGTAGG15650.081.854311
AGTTCGG3350.081.698012
GAGTCGA4900.080.253741
GAGTGGT12100.078.786531
GAGTAAC12150.076.0103761
AGTCCGG4500.075.363462
GAGTAGC8600.074.824311
Produced by FastQC (version 0.11.5)