##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935977_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 835404 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.959317886914594 31.0 31.0 34.0 25.0 34.0 2 30.096152280812635 31.0 31.0 34.0 25.0 34.0 3 30.2480201196068 33.0 31.0 34.0 25.0 34.0 4 33.061492403675345 37.0 35.0 37.0 23.0 37.0 5 33.38605632723808 37.0 35.0 37.0 27.0 37.0 6 33.60010485944525 37.0 35.0 37.0 27.0 37.0 7 33.51087258380377 37.0 35.0 37.0 26.0 37.0 8 33.403468262062425 37.0 35.0 37.0 25.0 37.0 9 34.79611421539758 39.0 37.0 39.0 18.0 39.0 10-11 34.64604371058793 39.0 37.0 39.0 22.0 39.0 12-13 34.53421518211548 39.0 35.0 39.0 22.0 39.0 14-15 35.999396699082126 40.0 37.0 41.0 24.0 41.0 16-17 36.30497998573145 40.0 37.0 41.0 26.0 41.0 18-19 36.465737535372114 40.0 37.0 41.0 27.0 41.0 20-21 36.64715395186042 40.0 37.0 41.0 28.0 41.0 22-23 36.61245277733887 40.0 37.0 41.0 28.0 41.0 24-25 36.56293422104754 40.0 36.0 41.0 28.0 41.0 26-27 36.56231057069394 40.0 36.0 41.0 28.5 41.0 28-29 36.46235114986282 40.0 36.0 41.0 28.0 41.0 30-31 36.38314994900671 40.0 36.0 41.0 28.0 41.0 32-33 36.328688873886165 40.0 35.5 41.0 28.5 41.0 34-35 36.06417852919067 40.0 35.0 41.0 28.0 41.0 36-37 36.03134172208895 40.0 35.0 41.0 28.0 41.0 38-39 36.1585765689415 40.0 35.0 41.0 28.5 41.0 40-41 36.088503885545194 40.0 35.0 41.0 29.0 41.0 42-43 35.8383129599571 40.0 35.0 41.0 28.0 41.0 44-45 35.60534124806681 40.0 35.0 41.0 28.0 41.0 46-47 35.429796840809956 39.5 35.0 41.0 28.0 41.0 48-49 35.23199015087311 39.0 35.0 41.0 27.5 41.0 50-51 34.997879469095196 39.0 35.0 41.0 27.0 41.0 52-53 34.71989360836194 38.5 34.5 41.0 26.5 41.0 54-55 34.4072789931578 38.0 34.0 40.0 26.0 41.0 56-57 34.13177815763391 37.0 34.0 40.0 26.0 41.0 58-59 33.78078450665785 37.0 34.0 39.5 25.0 41.0 60-61 33.458705608304484 36.0 34.0 39.0 25.0 41.0 62-63 32.97976667576406 36.0 33.5 39.0 22.5 41.0 64-65 32.465850055781395 35.0 33.0 37.5 20.5 40.0 66-67 32.11115161047829 35.0 33.0 37.0 20.0 39.5 68-69 31.862348636108997 35.0 33.0 36.5 19.5 39.0 70-71 31.676115986995512 35.0 33.0 36.0 19.0 39.0 72-73 31.45786350077328 35.0 33.0 35.5 18.5 37.5 74-75 31.16456409114632 35.0 33.0 35.0 14.5 37.0 76-77 30.96850805119439 35.0 33.0 35.0 10.5 37.0 78-79 30.75854915705455 35.0 32.0 35.0 7.0 36.0 80-81 30.56670425327147 35.0 32.0 35.0 2.0 36.0 82-83 30.416465566360706 35.0 32.0 35.0 2.0 36.0 84-85 30.226303680614407 35.0 32.0 35.0 2.0 35.0 86-87 30.03673432255531 35.0 32.0 35.0 2.0 35.0 88-89 29.751953545829323 35.0 31.0 35.0 2.0 35.0 90-91 29.540837726417397 35.0 31.0 35.0 2.0 35.0 92-93 29.385457814422722 35.0 30.0 35.0 2.0 35.0 94-95 29.18538874604383 35.0 30.0 35.0 2.0 35.0 96-97 29.027149738330195 35.0 30.0 35.0 2.0 35.0 98-99 28.792791870759537 35.0 29.5 35.0 2.0 35.0 100-101 28.582904199644723 35.0 29.0 35.0 2.0 35.0 102-103 27.87456069159353 34.0 27.5 35.0 2.0 35.0 104-105 28.349086789146327 34.5 29.0 35.0 2.0 35.0 106-107 28.278426964678168 35.0 29.0 35.0 2.0 35.0 108-109 28.081980095857812 35.0 28.5 35.0 2.0 35.0 110-111 27.937596061306866 35.0 28.0 35.0 2.0 35.0 112-113 27.725083312983898 34.5 27.5 35.0 2.0 35.0 114-115 27.409981278519133 34.0 27.0 35.0 2.0 35.0 116-117 27.099048484326147 34.0 25.5 35.0 2.0 35.0 118-119 26.774342114713363 34.0 25.0 35.0 2.0 35.0 120-121 26.36583198069437 34.0 24.5 35.0 2.0 35.0 122-123 25.74060095474764 34.0 20.0 35.0 2.0 35.0 124-125 22.814244964113172 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4470662031908894 1101 2 0.4671844543700914 1101 3 0.48920988728165327 1101 4 0.33094277782223 1101 5 0.32232415468165243 1101 6 0.34962157120008186 1101 7 0.33143733313154655 1101 8 0.3438506821516967 1101 9 0.44817827403362287 1101 10-11 0.5550371796652058 1101 12-13 0.45424991085212696 1101 14-15 0.3890776730766561 1101 16-17 0.34013116229632345 1101 18-19 0.32510904920686556 1101 20-21 0.37442725249949405 1101 22-23 0.4318767084404058 1101 24-25 0.5147170140865569 1101 26-27 0.5827512606791316 1101 28-29 0.5026559272370719 1101 30-31 0.45368062346643967 1101 32-33 0.5519586929863536 1101 34-35 0.2665475761600504 1101 36-37 0.29263835529982885 1101 38-39 0.43273372782187636 1101 40-41 0.3040090156894877 1101 42-43 0.27175251324150196 1101 44-45 0.2795324761321467 1101 46-47 0.43788897312803243 1101 48-49 0.3536394135423464 1101 50-51 0.47606714535614003 1101 52-53 0.4551186041968691 1101 54-55 0.40209511007421384 1101 56-57 0.431778506363095 1101 58-59 0.49508520890391594 1101 60-61 0.4511812253842393 1101 62-63 0.5933316768326193 1101 64-65 0.48225771716634824 1101 66-67 0.3648392239628535 1101 68-69 0.436147653482049 1101 70-71 0.4247827294545736 1101 72-73 0.5297317100217107 1101 74-75 0.5158304963670233 1101 76-77 0.6766892463074043 1101 78-79 0.7439501263290076 1101 80-81 0.7101153969662768 1101 82-83 0.6332990626097796 1101 84-85 0.4780873126639378 1101 86-87 0.4290808918893845 1101 88-89 0.4049432411725178 1101 90-91 0.48999492335652306 1101 92-93 0.0949007287929966 1101 94-95 -9.617848792231598E-5 1101 96-97 -0.06558864492957284 1101 98-99 -0.02443488412547623 1101 100-101 0.07806183561245916 1101 102-103 0.31669721076706736 1101 104-105 0.14277864123635808 1101 106-107 0.2248298282676906 1101 108-109 0.16211626284312075 1101 110-111 0.18774541751578155 1101 112-113 0.00791078276256485 1101 114-115 -0.00466845338816313 1101 116-117 0.05840831367523336 1101 118-119 0.10677171574096533 1101 120-121 0.03496641253111221 1101 122-123 0.037544652039620985 1101 124-125 0.30838419305434783 1102 1 0.21747377220922814 1102 2 0.1674038951337451 1102 3 0.3476840987719072 1102 4 0.2904297928370383 1102 5 0.06989300422124956 1102 6 0.1652913297308416 1102 7 0.11439353781914008 1102 8 0.403166364570275 1102 9 0.2712946704786816 1102 10-11 0.2108325258330126 1102 12-13 0.18447323402929783 1102 14-15 0.23837546263297327 1102 16-17 0.2493008322067638 1102 18-19 0.23343260617284756 1102 20-21 0.11647210281557108 1102 22-23 0.09861877270279251 1102 24-25 0.07819363098518295 1102 26-27 0.19372667487357376 1102 28-29 0.05679069762607014 1102 30-31 0.051773176828532996 1102 32-33 0.15960957961143407 1102 34-35 -0.04586348703044507 1102 36-37 0.03021843007848446 1102 38-39 -0.004263562928692011 1102 40-41 0.0015086569882427625 1102 42-43 -0.19099235311063012 1102 44-45 -0.10050094638415885 1102 46-47 -0.157261807996683 1102 48-49 -0.26089094102329113 1102 50-51 -0.33305272838101985 1102 52-53 -0.15546561463621345 1102 54-55 -0.03918211957054041 1102 56-57 -0.038155155006052155 1102 58-59 -0.013530795079780944 1102 60-61 -0.18069845355704928 1102 62-63 -0.09590475371015827 1102 64-65 -0.14560492979565964 1102 66-67 -0.175507492443721 1102 68-69 0.12703242176104723 1102 70-71 -0.007207935872937554 1102 72-73 -0.12254667590707058 1102 74-75 -0.14481828869074675 1102 76-77 -0.08065737725884858 1102 78-79 -0.27217075177164674 1102 80-81 -0.14026018434075027 1102 82-83 -0.10859724318936514 1102 84-85 -0.06607049900524942 1102 86-87 -0.08324492700446484 1102 88-89 -0.13682857419340166 1102 90-91 -0.15098141924115183 1102 92-93 -0.10419070204518377 1102 94-95 -0.08598191737029737 1102 96-97 -0.10403086870851297 1102 98-99 -0.06971521970104533 1102 100-101 0.08584016668105221 1102 102-103 -0.03109867556261392 1102 104-105 -0.22282148705209082 1102 106-107 -0.28482978534776393 1102 108-109 -0.08905901124683169 1102 110-111 -0.12498552278788821 1102 112-113 -0.06200473254342853 1102 114-115 -0.1755288299822766 1102 116-117 -0.22412462353938878 1102 118-119 -0.23831956317668457 1102 120-121 -0.40783761842691746 1102 122-123 -0.7292244203167506 1102 124-125 -0.8836475752162087 1103 1 0.3712142240541958 1103 2 0.2811289890997166 1103 3 0.4106109871847927 1103 4 0.4546903917943226 1103 5 0.341355640118266 1103 6 0.41154279479166433 1103 7 0.24752209943252979 1103 8 0.43765288601068164 1103 9 0.43773770487224795 1103 10-11 0.5489321903845976 1103 12-13 0.4464038810809967 1103 14-15 0.48841991965111475 1103 16-17 0.4427030065591069 1103 18-19 0.3441952461114113 1103 20-21 0.2922794018496475 1103 22-23 0.3490411143751899 1103 24-25 0.48258679268748494 1103 26-27 0.4835541008122206 1103 28-29 0.3939426193817184 1103 30-31 0.33846840376139653 1103 32-33 0.4651141667673997 1103 34-35 0.4551388166514059 1103 36-37 0.4286705175375616 1103 38-39 0.4858326258923924 1103 40-41 0.5462061471874904 1103 42-43 0.5071970337866674 1103 44-45 0.4546136314762421 1103 46-47 0.48729018650681866 1103 48-49 0.33081176277172375 1103 50-51 0.34782873304241946 1103 52-53 0.31539280996872776 1103 54-55 0.46299829509219137 1103 56-57 0.4263661237394487 1103 58-59 0.4626948040795753 1103 60-61 0.5018582291410851 1103 62-63 0.3199542771268469 1103 64-65 0.31360171450777585 1103 66-67 0.34576721151295686 1103 68-69 0.49344312377777655 1103 70-71 0.4891728586952162 1103 72-73 0.4136655861468448 1103 74-75 0.4663020281665453 1103 76-77 0.517988704461235 1103 78-79 0.4817050919013113 1103 80-81 0.5263707819931618 1103 82-83 0.5446016413436361 1103 84-85 0.4973716866634277 1103 86-87 0.5546647848332249 1103 88-89 0.5961322632151607 1103 90-91 0.5806315622081506 1103 92-93 0.7207557474556836 1103 94-95 0.7097300011521135 1103 96-97 0.6111229121588053 1103 98-99 0.7269404000702053 1103 100-101 0.4931755746752309 1103 102-103 0.6659833274281262 1103 104-105 0.6687114717645422 1103 106-107 0.5125270428460702 1103 108-109 0.4969208618485865 1103 110-111 0.5723025991051713 1103 112-113 0.466653877972675 1103 114-115 0.18404963665475194 1103 116-117 0.30477445902941724 1103 118-119 0.3592898617111615 1103 120-121 0.06800403375753916 1103 122-123 0.22348947856373869 1103 124-125 -0.2559629775522225 1104 1 0.05274637806361682 1104 2 -0.04176246678252582 1104 3 0.1819804433571548 1104 4 0.110936049495173 1104 5 0.16719795071767862 1104 6 0.034012809068762806 1104 7 -0.005277967753812618 1104 8 0.05494955188841999 1104 9 0.27145685068951764 1104 10-11 0.2081955074048807 1104 12-13 0.2538482042167587 1104 14-15 0.3559227254444082 1104 16-17 0.410946007345494 1104 18-19 0.3465906682783242 1104 20-21 0.3121902272491397 1104 22-23 0.32476317669051724 1104 24-25 0.341463788236382 1104 26-27 0.5779951944226482 1104 28-29 0.4009697000587735 1104 30-31 0.38210326125764027 1104 32-33 0.36052144579686285 1104 34-35 0.4013309543223329 1104 36-37 0.3904063683228003 1104 38-39 0.18940266383740578 1104 40-41 0.29502542356004113 1104 42-43 0.18574512164808255 1104 44-45 0.17328150953303378 1104 46-47 0.42825247817189194 1104 48-49 0.4887775435457371 1104 50-51 0.46118430912713393 1104 52-53 0.18519789322634495 1104 54-55 0.2269142663547541 1104 56-57 0.29045693218966306 1104 58-59 0.12947955583367587 1104 60-61 0.09902266008040073 1104 62-63 0.039190566913759994 1104 64-65 0.12038333598908224 1104 66-67 0.21881938018121616 1104 68-69 0.22523333442223148 1104 70-71 0.317105580734637 1104 72-73 0.3350781790052402 1104 74-75 0.3940278468092302 1104 76-77 0.33107286299046734 1104 78-79 0.28415586645295576 1104 80-81 0.3121001737277176 1104 82-83 0.43552690417202555 1104 84-85 0.4142961554714013 1104 86-87 0.3753100791170425 1104 88-89 0.5658052642305904 1104 90-91 0.43655693955871655 1104 92-93 0.3278160395635794 1104 94-95 0.47239500851970817 1104 96-97 0.4250423190866357 1104 98-99 0.5148999202986388 1104 100-101 0.4379842844684063 1104 102-103 0.552533843159658 1104 104-105 0.39833110544627814 1104 106-107 0.3984457779104673 1104 108-109 0.4541930199808064 1104 110-111 0.45818430533288534 1104 112-113 0.46648094948795915 1104 114-115 0.6026587266615451 1104 116-117 0.3163862441247396 1104 118-119 0.6464839920679388 1104 120-121 0.40426786231020984 1104 122-123 0.3244755144931659 1104 124-125 0.5037683534244977 1105 1 0.11776866080687043 1105 2 0.13279895223915616 1105 3 0.20507815731275514 1105 4 0.21666707674143026 1105 5 0.20176125610915818 1105 6 0.1722188713897026 1105 7 0.14055904989552914 1105 8 0.2600112126012277 1105 9 0.17281592118079914 1105 10-11 0.3337238383213048 1105 12-13 0.16710835229679333 1105 14-15 0.32866910302490737 1105 16-17 0.3932915018691361 1105 18-19 0.3225490325599125 1105 20-21 0.3322704863891843 1105 22-23 0.40853420676632624 1105 24-25 0.3406429226284473 1105 26-27 0.3770327037071226 1105 28-29 0.40334018898224144 1105 30-31 0.5046892974052 1105 32-33 0.5686005613252192 1105 34-35 0.5879769298702655 1105 36-37 0.44758253168242845 1105 38-39 0.4593473401481347 1105 40-41 0.5277732453924102 1105 42-43 0.4859306638025558 1105 44-45 0.45837535643054395 1105 46-47 0.4871867971334396 1105 48-49 0.4441752138790349 1105 50-51 0.5464415922830312 1105 52-53 0.6290769179731583 1105 54-55 0.4927118765600227 1105 56-57 0.6131094333981153 1105 58-59 0.5708112444883398 1105 60-61 0.38098631209233247 1105 62-63 0.46625511023541577 1105 64-65 0.2971796611049129 1105 66-67 0.2782363445406162 1105 68-69 0.37257944791095454 1105 70-71 0.4026981366478104 1105 72-73 0.28057461848224463 1105 74-75 0.3023841702744896 1105 76-77 0.41855547301887697 1105 78-79 0.41008210349890106 1105 80-81 0.457582760176507 1105 82-83 0.2494099964037204 1105 84-85 0.21554499122753867 1105 86-87 0.2096285985525448 1105 88-89 0.27461903237216134 1105 90-91 0.2439305822566986 1105 92-93 0.28555840860824944 1105 94-95 0.3517248166994804 1105 96-97 0.2723421575662215 1105 98-99 0.37362482273795194 1105 100-101 0.3922840286295006 1105 102-103 0.3126782388728806 1105 104-105 0.32889869643414826 1105 106-107 0.2585375615910124 1105 108-109 0.29462381968370366 1105 110-111 0.29984050854207567 1105 112-113 0.23410008553059214 1105 114-115 0.2906362140169776 1105 116-117 0.1858118739954051 1105 118-119 -0.002158263794544979 1105 120-121 -0.16172265868155122 1105 122-123 -0.044163882340090055 1105 124-125 0.15333832005933345 1106 1 -0.39938590593786216 1106 2 -0.2869651829231046 1106 3 -0.1708976428433715 1106 4 -0.8718352094189399 1106 5 -0.45826160549449924 1106 6 -0.34091261071132806 1106 7 -0.08026731343668558 1106 8 0.05450491172022254 1106 9 0.006698490602005336 1106 10-11 -0.045604532371228856 1106 12-13 -0.026904416745253457 1106 14-15 -0.07739758594576784 1106 16-17 -0.007320464228790513 1106 18-19 -0.16965432419262783 1106 20-21 -0.1248235807458613 1106 22-23 0.0018485816966276047 1106 24-25 -0.10230343624296978 1106 26-27 -0.05519797544978644 1106 28-29 -0.0970933450871172 1106 30-31 -0.1761069555222008 1106 32-33 -0.05250878405227155 1106 34-35 -0.07859773278168802 1106 36-37 -0.01433338222412317 1106 38-39 0.03273448189008121 1106 40-41 -0.02002841625585461 1106 42-43 -0.06257078595767496 1106 44-45 -0.07236513648793164 1106 46-47 0.0015477874311997653 1106 48-49 -0.0926626360150209 1106 50-51 -0.07956348187778417 1106 52-53 0.04134969593680182 1106 54-55 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-1.2335279384161169 2216 88-89 -1.1681072629173244 2216 90-91 -1.275147128350408 2216 92-93 -0.9948002557322368 2216 94-95 -1.127713181163685 2216 96-97 -1.1124528141003793 2216 98-99 -1.0998819258859527 2216 100-101 -1.1478324155624904 2216 102-103 -1.1750526884881225 2216 104-105 -1.4305032253683727 2216 106-107 -1.2119338277599567 2216 108-109 -1.3308376969308924 2216 110-111 -1.7489627602803637 2216 112-113 -1.909305636599214 2216 114-115 -1.874197065219171 2216 116-117 -1.8268358789427452 2216 118-119 -2.062012573311595 2216 120-121 -1.8902596002971634 2216 122-123 -1.6986908024520737 2216 124-125 -1.4880091732123297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25306.0 3 2934.0 4 1821.0 5 1992.0 6 2750.0 7 3535.0 8 5807.0 9 4487.0 10 4704.0 11 4856.0 12 4348.0 13 3866.0 14 3790.0 15 3768.0 16 3583.0 17 3443.0 18 3360.0 19 3237.0 20 3193.0 21 3195.0 22 4045.0 23 5275.0 24 7999.0 25 11516.0 26 13072.0 27 10911.0 28 11936.0 29 15239.0 30 17341.0 31 21584.0 32 29430.0 33 39809.0 34 60811.0 35 80108.0 36 164779.0 37 212723.0 38 34727.0 39 124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 73.70540140979325 9.463767785780488 6.111222352381169 10.719608452045087 2 10.338052855778002 36.436700692244514 42.24415567001176 10.981090781965726 3 41.57133485289611 20.959790645111863 17.344564051486362 20.12431045050567 4 14.04830545405786 18.113228329111138 48.36603341681951 19.472432800011493 5 16.526908403571586 33.063632805065055 27.189955121736332 23.219503669627027 6 28.91319648936323 40.757046889888 14.109101704085688 16.220654916663076 7 45.01617185498718 24.39424372936622 15.054189480034763 15.535394935611837 8 42.991278450374786 24.325294887970102 15.196755573963195 17.486671087691914 9 59.11882155220708 12.127665177566783 9.06962379878478 19.68388947144136 10-11 41.327549305485725 27.402609994685207 16.31246678253875 14.957373917290317 12-13 27.93438863112937 32.26726230662051 21.718114828274704 18.080234233975418 14-15 24.567215383215785 31.78156915695879 20.681909591048164 22.969305868777262 16-17 23.06285342181747 34.73648677765488 22.1003251121613 20.10033468836635 18-19 22.90191332576813 27.321092549233665 26.102879564857247 23.674114560140964 20-21 23.373900531958192 28.640514050686853 22.93680662290341 25.048778794451547 22-23 26.10659034431245 23.11636046751033 24.143887268914202 26.633161919263017 24-25 24.052135254320063 22.192496085726187 32.38516933124572 21.370199328708026 26-27 18.592391268566804 24.04220515911326 30.2724877019328 27.09291587038714 28-29 21.09262770786646 25.90202359746404 22.846156792890117 30.159191901779387 30-31 21.10368157202982 29.609925257719617 21.5416133990261 27.744779771224458 32-33 28.463653513749037 27.935705359323155 19.427486581342677 24.173154545585128 34-35 27.26650818047316 22.039995020373375 24.627784880129852 26.06571191902361 36-37 23.445482664674817 28.681931137509515 27.106824961336073 20.765761236479598 38-39 21.681844951664107 32.67568745181972 22.89742447965296 22.74504311686322 40-41 21.577823424355163 32.89504239864784 22.384379294329452 23.14275488266755 42-43 21.528266563243655 26.92565513212769 20.739725929011595 30.80635237561707 44-45 22.78801633700581 26.043985903826172 21.710932674490426 29.45706508467759 46-47 25.447747437168122 24.18291030447544 23.205000215464615 27.16434204289182 48-49 18.632721413830915 24.936258385164543 25.755263321698248 30.6757568793063 50-51 23.319196460634615 26.68966152903266 24.46504924563445 25.526092764698276 52-53 26.103418226391064 23.47504919775342 26.94732129604359 23.474211279811925 54-55 20.399411542199942 24.64238859282455 28.93133142766853 26.026868437306977 56-57 19.47616898670462 27.317594023483277 26.093394445555017 27.11284254425708 58-59 20.638816668342503 26.030339811636043 31.198857079927794 22.131986440093655 60-61 22.43676259436308 29.85694936638403 28.15417938756541 19.552108651687476 62-63 24.549200147473556 26.41290920321186 26.856107942983275 22.181782706331308 64-65 28.90213611525087 21.66549955410315 28.853476499141124 20.578887831504858 66-67 29.0880993436106 24.456445298589244 24.98373540450812 21.47171995329203 68-69 22.193112876426337 28.996140567614287 25.102351572263803 23.708394983695573 70-71 21.91397951163388 30.6493410092373 22.297575170202617 25.139104308926203 72-73 20.8552513845106 29.93954418433727 23.45411528680452 25.75108914434761 74-75 20.07074984661888 29.22531312194173 26.792464123782306 23.911472907657085 76-77 20.803098388560237 28.189183171694587 25.242757379082164 25.764961060663012 78-79 20.9542637162167 29.438183955432024 23.690504406282994 25.91704792206828 80-81 18.947675239825806 23.420154225889934 27.965472744727666 29.666697789556594 82-83 20.753204888416196 20.441678781570822 29.43374070134971 29.371375628663277 84-85 21.9209497475916 21.522627561572623 31.255540982468194 25.30088170836758 86-87 24.06669004904314 23.167047129055156 25.208196919511554 27.55806590239015 88-89 21.279871561489276 25.871356763464465 26.045613150820067 26.80315852422619 90-91 19.215336789549475 28.08500807394754 25.663901135140765 27.035754001362218 92-93 20.576036356039694 25.49430171035485 26.516097017279534 27.413564916325917 94-95 18.954814874230365 23.833798038302547 28.283173529894157 28.928213557572935 96-97 20.115710314809625 22.935505804669113 28.298330544123385 28.650453336397874 98-99 20.099271474504 23.190477216520268 30.25379884269068 26.456452466285047 100-101 18.51397865808952 26.227902013230814 29.021060009085488 26.23705931959418 102-103 18.52564747116365 27.2969724827748 28.615077256034205 25.562302790027342 104-105 18.051989403891067 27.134453992753183 27.96860429901329 26.844952304342467 106-107 17.89834874619267 28.587597132136818 25.397491367861093 28.116562753809422 108-109 19.285818597947816 28.84951472580931 25.609046640906673 26.255620035336197 110-111 19.38930146372294 29.240642850644715 25.192541572700154 26.177514112932187 112-113 18.40836874075661 29.0012682630781 26.348210336553567 26.24215265961172 114-115 19.806730610962305 26.973918508431964 26.82482155950261 26.394529321103118 116-117 22.189204265241727 26.55721064299429 25.086125994129787 26.1674590976342 118-119 20.825801381686365 26.488750887961693 25.778621116777295 26.90682661357464 120-121 19.59608159767007 27.039118784426226 26.19538264394598 27.169416973957723 122-123 20.541149754689393 28.115342970984695 25.923558377460516 25.419948896865396 124-125 19.956356532450304 30.16073203906912 23.50945318467115 26.373458243809427 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 331.0 1 384.5 2 466.0 3 444.0 4 357.5 5 293.0 6 245.5 7 216.0 8 207.5 9 210.5 10 220.0 11 228.0 12 246.5 13 268.0 14 294.0 15 370.0 16 468.5 17 547.0 18 645.0 19 787.0 20 963.0 21 1195.0 22 1396.0 23 1636.0 24 1958.5 25 2326.5 26 2780.5 27 3294.5 28 3837.5 29 4360.5 30 5026.5 31 5589.5 32 6100.0 33 6813.0 34 7542.5 35 7919.0 36 8376.5 37 9130.5 38 9467.0 39 9569.5 40 9607.0 41 9799.5 42 9930.5 43 10059.5 44 10768.5 45 12617.5 46 18665.5 47 32631.5 48 50692.0 49 71748.0 50 87743.0 51 85835.0 52 70673.0 53 51437.5 54 36383.0 55 30081.5 56 26666.0 57 21861.0 58 17500.0 59 13419.0 60 10280.5 61 8215.5 62 6472.0 63 4881.0 64 3529.5 65 2961.0 66 2709.5 67 2398.0 68 1987.5 69 1467.0 70 1116.5 71 902.5 72 813.5 73 746.5 74 671.5 75 579.0 76 375.5 77 228.0 78 172.0 79 129.5 80 106.0 81 76.5 82 48.0 83 23.5 84 11.0 85 11.0 86 7.0 87 2.0 88 1.5 89 2.5 90 3.0 91 3.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16530923960143834 2 0.017955384460692073 3 0.009097394793417318 4 0.016279548577694147 5 0.003950184581352256 6 0.0 7 2.3940512614256096E-4 8 2.3940512614256096E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0010174717861058842 28-29 0.007182153784276829 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.1970256307128048E-4 58-59 0.0 60-61 5.985128153564024E-5 62-63 0.0 64-65 0.0010773230676415244 66-67 5.985128153564024E-5 68-69 0.005745723027421463 70-71 0.01789553317915643 72-73 5.985128153564024E-5 74-75 0.007960220444240152 76-77 2.3940512614256096E-4 78-79 7.182153784276829E-4 80-81 7.182153784276829E-4 82-83 5.985128153564024E-5 84-85 0.01801523574222771 86-87 0.0020349435722117684 88-89 0.01681821011151491 90-91 1.1970256307128048E-4 92-93 0.013107430656305213 94-95 0.01280817424862701 96-97 0.00502750764899378 98-99 0.0021546461352830487 100-101 6.583640968920426E-4 102-103 0.0 104-105 3.5910768921384144E-4 106-107 0.0010174717861058842 108-109 0.0 110-111 0.0 112-113 0.0011371743491771647 114-115 0.00496765636745814 116-117 0.0 118-119 0.07505350704569286 120-121 0.001496282038391006 122-123 0.004129738425959177 124-125 0.24664713120837342 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 835404.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.569938365825166 #Duplication Level Percentage of deduplicated Percentage of total 1 83.6619151895646 38.12468318755351 2 8.747095175887468 7.972091760903971 3 2.5396221110370223 3.471912692173317 4 1.1871168000572903 2.1638735764658525 5 0.716689573025467 1.6329749835100043 6 0.480601930593461 1.3140600213384361 7 0.3535415038084453 1.1277605176818397 8 0.2718256788487019 0.9909663545107146 9 0.21014364441833397 0.8618609631702023 >10 1.5269634195264497 13.821654424401236 >50 0.17560391001222195 5.538901928352423 >100 0.11129444155912652 9.12184860929012 >500 0.009449528056876 2.891097310334682 >1k 0.006562172261719444 5.757314676914725 >5k 0.0013124344523438888 3.9361745631437057 >10k+ 2.6248689046877774E-4 1.272824430255331 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 10641 1.2737549736414955 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 8396 1.005022719546471 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 7080 0.8474941465446658 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6199 0.7420361884788677 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 6154 0.73664957314066 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 5078 0.6078496152759623 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 4561 0.5459633901681102 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3624 0.4338020885703205 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3295 0.3944199453198692 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2698 0.32295751516631477 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 2692 0.322239299787887 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2534 0.30332629482262474 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 2122 0.25400883883725717 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1944 0.23270178261056929 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1943 0.23258208004749797 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1927 0.2306668390383575 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1920 0.22982892109685854 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1917 0.2294698134076447 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1811 0.21678134172208896 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1676 0.2006214957074661 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1593 0.1906861829725498 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1407 0.16842150624129165 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1390 0.16638656266907986 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1217 0.14567801925774834 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1178 0.1410096192979684 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1174 0.1405308090456833 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1148 0.13741854240582999 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1121 0.13418657320290542 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1097 0.1313137116891947 No Hit GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG 1091 0.130595496310767 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1052 0.12592709635098706 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 992 0.11874494256671024 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 934 0.11180219390857597 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 931 0.11144308621936212 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 917 0.10976725033636421 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 884 0.10581706575501194 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.1970256307128048E-4 0.0 0.0 0.0 0.0 3 1.1970256307128048E-4 0.0 0.0 0.0 0.0 4 1.1970256307128048E-4 0.0 0.0 0.0 0.0 5 1.1970256307128048E-4 0.0 0.0 0.0 0.0 6 1.1970256307128048E-4 0.0 0.0 0.0 0.0 7 1.1970256307128048E-4 0.0 0.0 0.0 0.0 8 1.1970256307128048E-4 0.0 0.0 0.0 0.0 9 1.1970256307128048E-4 0.0 0.0 1.1970256307128048E-4 0.0 10-11 1.1970256307128048E-4 0.0 0.0 1.1970256307128048E-4 0.0 12-13 1.1970256307128048E-4 0.0 0.0 1.1970256307128048E-4 0.0 14-15 1.1970256307128048E-4 0.0 0.0 2.3940512614256096E-4 0.0 16-17 1.1970256307128048E-4 0.0 0.0 2.3940512614256096E-4 0.0 18-19 1.1970256307128048E-4 0.0 0.0 4.788102522851219E-4 0.0 20-21 1.1970256307128048E-4 0.0 0.0 0.001197025630712805 0.0 22-23 1.1970256307128048E-4 0.0 0.0 0.0035910768921384147 0.0 24-25 1.1970256307128048E-4 0.0 0.0 0.007421558910419389 0.0 26-27 1.1970256307128048E-4 0.0 0.0 0.01262862040402009 0.0 28-29 1.1970256307128048E-4 0.0 0.0 0.026035307468003505 0.0 30-31 1.1970256307128048E-4 0.0 0.0 0.07014570195977037 0.0 32-33 1.1970256307128048E-4 0.0 0.0 0.15603229096341412 0.0 34-35 1.1970256307128048E-4 0.0 0.0 0.26304638234913885 0.0 36-37 1.1970256307128048E-4 0.0 0.0 0.3992080478427204 0.0 38-39 1.1970256307128048E-4 0.0 0.0 0.6187425485154487 0.0 40-41 1.1970256307128048E-4 0.0 0.0 0.9449918841662237 0.0 42-43 1.1970256307128048E-4 0.0 0.0 1.6760154368425337 0.0 44-45 1.1970256307128048E-4 0.0 0.0 2.7199414893871707 0.0 46-47 1.1970256307128048E-4 0.0 0.0 3.7175426500232223 0.0 48-49 1.1970256307128048E-4 0.0 0.0 4.053607595845842 0.0 50-51 1.1970256307128048E-4 0.0 0.0 4.510871386778134 0.0 52-53 1.1970256307128048E-4 0.0 0.0 4.868782050361263 0.0 54-55 1.1970256307128048E-4 0.0 0.0 5.242553303551336 0.0 56-57 1.1970256307128048E-4 0.0 0.0 5.926414046377561 0.0 58-59 1.1970256307128048E-4 0.0 0.0 6.63864429665168 0.0 60-61 1.1970256307128048E-4 0.0 0.0 7.70531383617986 0.0 62-63 1.1970256307128048E-4 0.0 0.0 9.053523804051693 0.0 64-65 1.1970256307128048E-4 0.0 0.0 10.170707825195954 0.0 66-67 1.1970256307128048E-4 0.0 0.0 11.015508664071515 0.0 68-69 1.1970256307128048E-4 0.0 0.0 11.692426658239606 0.0 70-71 1.1970256307128048E-4 0.0 0.0 12.494493682098721 0.0 72-73 1.1970256307128048E-4 0.0 0.0 13.304760331528218 0.0 74-75 1.1970256307128048E-4 0.0 0.0 14.21120799038549 0.0 76-77 1.1970256307128048E-4 0.0 0.0 15.331264873043462 0.0 78-79 1.1970256307128048E-4 0.0 0.0 16.699525020229732 0.0 80-81 1.1970256307128048E-4 0.0 0.0 18.675455228847362 0.0 82-83 1.1970256307128048E-4 0.0 0.0 20.073940273209132 0.0 84-85 1.1970256307128048E-4 0.0 0.0 20.901384240439356 0.0 86-87 1.1970256307128048E-4 0.0 0.0 21.69782524383412 0.0 88-89 1.1970256307128048E-4 0.0 0.0 22.81339328037692 0.0 90-91 1.1970256307128048E-4 0.0 0.0 24.35611991323958 0.0 92-93 1.1970256307128048E-4 0.0 0.0 26.673322129173428 0.0 94-95 1.1970256307128048E-4 0.0 0.0 29.95089800862816 0.0 96-97 1.1970256307128048E-4 0.0 0.0 33.40018721480864 0.0 98-99 1.1970256307128048E-4 0.0 0.0 36.15945099616473 0.0 100-101 1.1970256307128048E-4 0.0 0.0 37.994610990610525 0.0 102-103 1.1970256307128048E-4 0.0 0.0 39.45558077289551 0.0 104-105 1.1970256307128048E-4 0.0 0.0 41.03906612848394 0.0 106-107 1.1970256307128048E-4 0.0 0.0 42.53355262842888 0.0 108-109 1.1970256307128048E-4 0.0 0.0 43.73333141809232 0.0 110-111 1.1970256307128048E-4 0.0 0.0 44.77175115273568 0.0 112-113 1.1970256307128048E-4 0.0 0.0 46.89042666781582 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTAGTA 1785 0.0 88.99682 2 GAGTAGT 2150 0.0 87.573875 1 GTGATCG 120 0.0 84.40995 1 AGTTCGC 50 9.367795E-10 83.29702 2 GAGTCAG 4440 0.0 83.06798 1 GAGTGCG 275 0.0 82.33356 1 GAGTAAG 820 0.0 81.38233 1 AGTCCGT 200 0.0 80.32213 2 GAGTACG 475 0.0 80.28092 1 GAGTCCG 480 0.0 78.20334 1 GAGTCGG 555 0.0 77.2975 1 GAGTTAG 965 0.0 75.9458 1 GAGTCGT 730 0.0 75.90774 1 GCTTCGA 175 0.0 74.77047 9 GAGTCGA 375 0.0 74.677986 1 GAGTAAC 880 0.0 74.47936 1 AGTCAGT 3420 0.0 73.58948 2 AGTCGAC 130 0.0 73.22815 2 GAGTCGC 270 0.0 72.824265 1 GTATCCG 205 0.0 72.662796 1 >>END_MODULE