##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935977_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 835404 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50961331284025 33.0 31.0 34.0 31.0 34.0 2 32.546812081340285 34.0 31.0 34.0 31.0 34.0 3 32.76448520715726 34.0 31.0 34.0 31.0 34.0 4 35.96396952851554 37.0 35.0 37.0 35.0 37.0 5 36.02948752938698 37.0 35.0 37.0 35.0 37.0 6 36.16719096389292 37.0 36.0 37.0 35.0 37.0 7 36.205318624282384 37.0 37.0 37.0 35.0 37.0 8 36.205116326950794 37.0 37.0 37.0 35.0 37.0 9 38.0278320429397 39.0 39.0 39.0 35.0 39.0 10-11 37.739934211471336 39.0 38.0 39.0 35.0 39.0 12-13 37.644212859885755 39.0 37.0 39.0 35.0 39.0 14-15 39.202215335334756 41.0 39.0 41.0 35.5 41.0 16-17 39.30839450134306 41.0 39.0 41.0 36.0 41.0 18-19 39.3080491594486 41.0 39.0 41.0 35.0 41.0 20-21 39.29096401262144 41.0 39.0 41.0 35.0 41.0 22-23 39.06487878918463 41.0 38.5 41.0 35.0 41.0 24-25 38.720578307022706 40.5 38.0 41.0 35.0 41.0 26-27 38.58563401659556 40.0 37.5 41.0 35.0 41.0 28-29 38.44048747671785 40.0 37.0 41.0 35.0 41.0 30-31 38.29235316086588 40.0 37.0 41.0 35.0 41.0 32-33 38.06814547213085 40.0 36.5 41.0 34.0 41.0 34-35 37.51225814097131 40.0 35.5 41.0 33.0 41.0 36-37 36.97501448401013 40.0 36.0 41.0 32.5 41.0 38-39 36.236908130676895 40.0 36.0 41.0 30.0 41.0 40-41 35.7727381003682 40.0 35.0 41.0 26.5 41.0 42-43 35.53442047201115 40.0 35.0 41.0 25.0 41.0 44-45 35.32189635194469 40.0 35.0 41.0 24.0 41.0 46-47 35.103337427160994 40.0 35.0 41.0 23.0 41.0 48-49 34.82476981197121 39.5 35.0 41.0 21.0 41.0 50-51 34.50930866981724 39.0 35.0 41.0 17.5 41.0 52-53 34.215309598709126 39.0 35.0 41.0 14.0 41.0 54-55 33.924908188134125 38.5 35.0 41.0 11.0 41.0 56-57 33.67029485135336 37.5 35.0 40.0 8.0 41.0 58-59 33.35008032041982 37.0 35.0 39.5 7.0 41.0 60-61 33.03878782002481 36.5 34.0 39.0 4.0 41.0 62-63 32.58660001627955 36.0 34.0 39.0 2.0 41.0 64-65 32.12305004524757 35.0 34.0 37.5 2.0 40.5 66-67 31.702204562104082 35.0 33.0 37.0 2.0 39.5 68-69 31.364431460706435 35.0 33.0 36.5 2.0 39.0 70-71 31.186196139831747 35.0 33.0 36.0 2.0 39.0 72-73 30.960168373625216 35.0 33.0 35.5 2.0 37.5 74-75 30.692411096906408 35.0 33.0 35.0 2.0 37.0 76-77 30.542772119836627 35.0 33.0 35.0 2.0 37.0 78-79 30.540270336268442 35.0 33.0 35.0 2.0 36.0 80-81 30.463855811080627 35.0 33.0 35.0 2.0 36.0 82-83 30.370019774863422 35.0 33.0 35.0 2.0 36.0 84-85 30.238534289996217 35.0 33.0 35.0 2.0 35.0 86-87 30.152958329143743 35.0 33.0 35.0 2.0 35.0 88-89 29.98984443454903 35.0 33.0 35.0 2.0 35.0 90-91 29.91657748825718 35.0 33.0 35.0 2.0 35.0 92-93 29.917900201579116 35.0 33.0 35.0 2.0 35.0 94-95 29.862395320108597 35.0 32.5 35.0 2.0 35.0 96-97 29.835381444187483 35.0 33.0 35.0 2.0 35.0 98-99 29.73069137806379 35.0 32.0 35.0 2.0 35.0 100-101 29.683218538575346 35.0 32.0 35.0 2.0 35.0 102-103 29.02923735103016 34.0 30.5 35.0 2.0 35.0 104-105 29.618538455645414 35.0 32.0 35.0 2.0 35.0 106-107 29.685653887221033 35.0 32.0 35.0 2.0 35.0 108-109 29.60617437790578 35.0 32.5 35.0 2.0 35.0 110-111 29.510002944683052 35.0 32.0 35.0 2.0 35.0 112-113 29.41753630578738 35.0 32.0 35.0 2.0 35.0 114-115 29.24018498834097 35.0 32.0 35.0 2.0 35.0 116-117 29.026958812742098 35.0 31.5 35.0 2.0 35.0 118-119 28.740037754188393 35.0 31.0 35.0 2.0 35.0 120-121 28.361648974627848 35.0 31.0 35.0 2.0 35.0 122-123 27.942556535520538 35.0 30.5 35.0 2.0 35.0 124-125 25.65860769160789 32.0 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11652891545134736 1101 2 0.15917513984123843 1101 3 0.11151654762839769 1101 4 0.24937095140037258 1101 5 0.23945046661755498 1101 6 0.1996872744343392 1101 7 0.22510042061832536 1101 8 0.16213057926323415 1101 9 0.30171540745458714 1101 10-11 0.22675371823932267 1101 12-13 0.28644623661644175 1101 14-15 0.33523481679451805 1101 16-17 0.2674124607317836 1101 18-19 0.32540790565607125 1101 20-21 0.3374348334080395 1101 22-23 0.4087277913805565 1101 24-25 0.4648712048523791 1101 26-27 0.3484081271575832 1101 28-29 0.32823930295531767 1101 30-31 0.328282698964415 1101 32-33 0.43239086138731864 1101 34-35 0.5799278145055027 1101 36-37 0.36177995539159724 1101 38-39 0.37929083295531285 1101 40-41 0.33834492456952603 1101 42-43 0.20944128972932674 1101 44-45 0.28202976994209195 1101 46-47 0.4238758090503367 1101 48-49 0.28778568489505574 1101 50-51 0.37195118044218134 1101 52-53 0.42513016321868236 1101 54-55 0.5475822070955161 1101 56-57 0.31289458292771855 1101 58-59 0.30672668847729767 1101 60-61 0.2282882369490551 1101 62-63 0.2968449485074558 1101 64-65 0.3106964852010705 1101 66-67 0.17808653113973705 1101 68-69 0.3120496795322403 1101 70-71 0.2979930668240591 1101 72-73 0.239638774141671 1101 74-75 0.2140811373864473 1101 76-77 0.312720341121441 1101 78-79 0.26459500476842734 1101 80-81 0.3598359430175577 1101 82-83 0.4590664299579714 1101 84-85 0.3899385105800377 1101 86-87 0.23982704939668764 1101 88-89 0.14385108814662217 1101 90-91 0.1447045443518391 1101 92-93 0.2734783718963385 1101 94-95 0.23976899529564477 1101 96-97 0.21074368182150494 1101 98-99 0.2444292328682529 1101 100-101 0.26094231716483307 1101 102-103 0.20398591782698716 1101 104-105 0.2564676861369044 1101 106-107 0.1962777913597904 1101 108-109 0.23756969432783137 1101 110-111 0.22731949485745062 1101 112-113 0.232686870452941 1101 114-115 0.17689515769765762 1101 116-117 0.06864977718236531 1101 118-119 -0.20672278006924927 1101 120-121 -0.04771130785522004 1101 122-123 0.1972663261174219 1101 124-125 0.07371933987986878 1102 1 -0.05495813332358068 1102 2 -0.03821773508596493 1102 3 -0.0046857767556929275 1102 4 0.08822547061409836 1102 5 0.15611192859944367 1102 6 0.11698258262200767 1102 7 -0.018316778473526085 1102 8 -0.0012953974591596307 1102 9 0.11546505930337503 1102 10-11 0.1089207801806964 1102 12-13 -0.011095604829165495 1102 14-15 -0.002037618913554695 1102 16-17 -0.022704686875890445 1102 18-19 -0.17039993175520607 1102 20-21 -0.1312632076611422 1102 22-23 -0.11644586222376319 1102 24-25 -0.008164167310717119 1102 26-27 -0.10833461230714647 1102 28-29 0.08663882018564095 1102 30-31 0.09028568673480919 1102 32-33 -0.047016310949636875 1102 34-35 0.04798019332778125 1102 36-37 0.1203951153723537 1102 38-39 0.12884773141330896 1102 40-41 0.002197966778112459 1102 42-43 0.1082850057054614 1102 44-45 -0.0077679225538744845 1102 46-47 -0.030841745366750217 1102 48-49 0.08005250798360208 1102 50-51 -0.07361907014229274 1102 52-53 0.1320670233589567 1102 54-55 0.11230035231466218 1102 56-57 0.12760133410721863 1102 58-59 0.006311439032231192 1102 60-61 0.06382160460518094 1102 62-63 0.09534051327202064 1102 64-65 0.027856330917337857 1102 66-67 0.1784055642499709 1102 68-69 0.18450564367899602 1102 70-71 0.09624935675595125 1102 72-73 -0.05902896722207274 1102 74-75 0.03136251952565772 1102 76-77 -0.05290552925658787 1102 78-79 -0.08069545884238494 1102 80-81 0.07869729865507935 1102 82-83 0.11506602483656181 1102 84-85 0.08867672618902844 1102 86-87 -0.008036779169437125 1102 88-89 0.09165035048805592 1102 90-91 0.03404520925735355 1102 92-93 0.037194685629110324 1102 94-95 0.05602195985596481 1102 96-97 0.13490242189392276 1102 98-99 0.13529923109584985 1102 100-101 0.078543154415744 1102 102-103 0.04741113494673854 1102 104-105 0.0994586637455086 1102 106-107 0.010841062039972371 1102 108-109 0.016484812952445083 1102 110-111 -0.01640238929470783 1102 112-113 -0.16174355416493924 1102 114-115 -0.23292566047879149 1102 116-117 -0.07150408455306945 1102 118-119 0.004218030004771833 1102 120-121 0.0564711486997318 1102 122-123 0.07348063534322335 1102 124-125 -0.13653402980185803 1103 1 0.013679461147582117 1103 2 0.08645995153247554 1103 3 0.1259936073125303 1103 4 0.1503602157945494 1103 5 0.10603715997801544 1103 6 0.17469183372332964 1103 7 0.1861612557179626 1103 8 0.07653671667412709 1103 9 0.1852342869637269 1103 10-11 0.27961148058081164 1103 12-13 0.12289582709097147 1103 14-15 0.11274029807307073 1103 16-17 0.22675778735924723 1103 18-19 0.10039584886961705 1103 20-21 0.040876387375838874 1103 22-23 0.1752224884208573 1103 24-25 0.21664486958861318 1103 26-27 0.3478853286862673 1103 28-29 0.29122395537538637 1103 30-31 0.38936825554215204 1103 32-33 0.32326215164584227 1103 34-35 0.328239256497163 1103 36-37 0.4505450567699256 1103 38-39 0.4319536603629146 1103 40-41 0.3820427713693064 1103 42-43 0.40092534842865035 1103 44-45 0.33350839802164245 1103 46-47 0.49499990681770356 1103 48-49 0.4425287638943587 1103 50-51 0.3664488278490623 1103 52-53 0.31850359127714967 1103 54-55 0.34069626226899175 1103 56-57 0.2948203455447995 1103 58-59 0.32061043268700473 1103 60-61 0.24564676727744938 1103 62-63 0.28474155599117523 1103 64-65 0.3038838942685871 1103 66-67 0.3891560795472273 1103 68-69 0.1835316308268773 1103 70-71 0.13816020022683162 1103 72-73 0.18081462490499334 1103 74-75 0.1456999078779191 1103 76-77 0.1347333989251176 1103 78-79 0.18189396038729555 1103 80-81 0.35714170717465876 1103 82-83 0.3362760640395699 1103 84-85 0.28024201738249843 1103 86-87 0.23696786860973518 1103 88-89 0.15540979760440976 1103 90-91 0.2668201354991169 1103 92-93 0.33225591506774066 1103 94-95 0.2384048560286196 1103 96-97 0.2801960402910417 1103 98-99 0.3838766065151624 1103 100-101 0.4040776490202802 1103 102-103 0.4100086372706322 1103 104-105 0.28621451388493924 1103 106-107 0.26234249678919497 1103 108-109 0.3272434113439111 1103 110-111 0.44449160092474926 1103 112-113 0.3900731212584141 1103 114-115 0.315859854616388 1103 116-117 0.26135167263078074 1103 118-119 0.14420913860114126 1103 120-121 0.18307463251333544 1103 122-123 0.2806826722829605 1103 124-125 0.4769627167527233 1104 1 0.08801962254750606 1104 2 0.07927864765933634 1104 3 0.13628380650476402 1104 4 0.05311027496433951 1104 5 0.19375045752953213 1104 6 0.12547478366187192 1104 7 0.07207960904177924 1104 8 0.10551617139587677 1104 9 0.1278670754259963 1104 10-11 0.18809413810606657 1104 12-13 0.0949440180223462 1104 14-15 0.170299525124733 1104 16-17 0.16291930943530986 1104 18-19 0.2273875803685641 1104 20-21 0.17625114210751036 1104 22-23 0.20279713779213182 1104 24-25 0.26591879899714144 1104 26-27 0.2542653590728179 1104 28-29 0.20790907283696924 1104 30-31 0.30288645311580353 1104 32-33 0.20994551310073462 1104 34-35 0.350974962220981 1104 36-37 0.42046428046226936 1104 38-39 0.2564255222644434 1104 40-41 0.2391790298534957 1104 42-43 -0.0032806693299178846 1104 44-45 0.07104609490434655 1104 46-47 0.13862941994576516 1104 48-49 0.06760835680619692 1104 50-51 0.12744066623536554 1104 52-53 0.12157698472189793 1104 54-55 0.161928291830975 1104 56-57 0.26365542597753944 1104 58-59 0.11295844267333166 1104 60-61 0.04920153559909579 1104 62-63 -0.015508865832174479 1104 64-65 0.03561951600947566 1104 66-67 0.09729478880596432 1104 68-69 0.165013013338573 1104 70-71 0.10104241798693181 1104 72-73 0.1692444495333696 1104 74-75 0.2942542562849155 1104 76-77 0.17965930307769185 1104 78-79 0.21922742605736545 1104 80-81 0.23243698351667064 1104 82-83 0.2585032913050078 1104 84-85 0.18553408710359776 1104 86-87 0.2600479043406523 1104 88-89 0.2297205649793348 1104 90-91 0.23946155129859648 1104 92-93 0.28364659601659525 1104 94-95 0.3811479675197731 1104 96-97 0.36869076081876173 1104 98-99 0.2735268557917614 1104 100-101 0.3663406835525329 1104 102-103 0.2877073523318252 1104 104-105 0.28380191339173066 1104 106-107 0.37855845507258223 1104 108-109 0.4338929505830258 1104 110-111 0.3610545613993317 1104 112-113 0.2031507901977072 1104 114-115 0.3316570434787245 1104 116-117 0.3359991352011136 1104 118-119 0.4170517316885842 1104 120-121 0.4323420723319309 1104 122-123 0.36015800802380227 1104 124-125 0.4115865877549467 1105 1 0.11569030953980075 1105 2 0.17360745370514508 1105 3 0.20681487855230074 1105 4 0.2074993269456229 1105 5 0.17236668738369332 1105 6 0.10014584792813253 1105 7 0.11855209751723805 1105 8 0.15902570345826916 1105 9 0.2157895927864928 1105 10-11 0.06292908074863135 1105 12-13 0.04734928688898776 1105 14-15 0.17769542109143543 1105 16-17 0.12944066667837717 1105 18-19 0.10281201357339143 1105 20-21 0.11354113609471028 1105 22-23 0.17471528113266288 1105 24-25 0.19144108763530454 1105 26-27 0.2497365978282886 1105 28-29 0.17215573337293222 1105 30-31 0.09200430996391873 1105 32-33 0.13001280344841604 1105 34-35 0.25332953489927235 1105 36-37 0.41669964635906354 1105 38-39 0.3832632590922813 1105 40-41 0.36991624675064827 1105 42-43 0.3424710195605414 1105 44-45 0.3515382311002426 1105 46-47 0.3867017858071833 1105 48-49 0.2722824274743303 1105 50-51 0.21654584903639318 1105 52-53 0.2448424087424641 1105 54-55 0.27485184079996117 1105 56-57 0.23500397406228046 1105 58-59 0.27192415200732967 1105 60-61 0.21187440370647437 1105 62-63 0.22854980616348541 1105 64-65 0.22528736755216983 1105 66-67 0.18388284690615464 1105 68-69 0.1377166528533067 1105 70-71 0.16274439513827943 1105 72-73 0.12165192898962474 1105 74-75 0.2306533826599022 1105 76-77 0.23523573642399498 1105 78-79 0.21042043705725888 1105 80-81 0.04442781104351923 1105 82-83 0.11128318142396765 1105 84-85 0.2609969845676119 1105 86-87 0.3284693370385128 1105 88-89 0.29280416063596704 1105 90-91 0.26713592260691144 1105 92-93 0.3514601165721274 1105 94-95 0.3558971392855348 1105 96-97 0.24692264282633047 1105 98-99 0.371923146717144 1105 100-101 0.3544676067654926 1105 102-103 0.39337943052185054 1105 104-105 0.3272031560378359 1105 106-107 0.2839430092408719 1105 108-109 0.35479068576028183 1105 110-111 0.36763696039012217 1105 112-113 0.406384293304324 1105 114-115 0.35398768284019155 1105 116-117 0.3442092650069384 1105 118-119 0.3079790298175382 1105 120-121 0.10626831527278213 1105 122-123 0.21669663734034827 1105 124-125 0.47417869565529713 1106 1 -0.0385195711337758 1106 2 -0.0012196902671917087 1106 3 0.05008185747502125 1106 4 0.03973697610364724 1106 5 0.11879804681270656 1106 6 0.11862192688430895 1106 7 0.053190215059338186 1106 8 0.08648471985171113 1106 9 0.006711863334054158 1106 10-11 0.07532174273146097 1106 12-13 0.06427663159736596 1106 14-15 0.05439191936287813 1106 16-17 0.006679431036580752 1106 18-19 0.09746741671825276 1106 20-21 -0.016159432657381956 1106 22-23 0.012573539189986604 1106 24-25 0.07800292548520105 1106 26-27 0.07805431835912202 1106 28-29 -0.07581042030165008 1106 30-31 -0.09464813790047799 1106 32-33 -0.052351699729904055 1106 34-35 -0.12442919154212717 1106 36-37 -0.12607803848536747 1106 38-39 -0.19522731746621247 1106 40-41 -0.292180887184891 1106 42-43 -0.287451195227014 1106 44-45 -0.34979303911276105 1106 46-47 -0.3976826236943225 1106 48-49 -0.18928498637645674 1106 50-51 -0.2128354936646275 1106 52-53 -0.29655382188093427 1106 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-0.4048410957465052 2216 9 -0.2963484632311406 2216 10-11 -0.43346162265002874 2216 12-13 -0.33334528716329714 2216 14-15 -0.4217952200954542 2216 16-17 -0.4489748943770593 2216 18-19 -0.4824536036078797 2216 20-21 -0.41973877626784883 2216 22-23 -0.6349826537072758 2216 24-25 -0.55706616814971 2216 26-27 -0.470572499965904 2216 28-29 -0.9029049569071574 2216 30-31 -0.9358802534041715 2216 32-33 -0.9609626026185794 2216 34-35 -1.0174361769520672 2216 36-37 -0.7916767956690478 2216 38-39 -0.8174880155726569 2216 40-41 -0.6416368834477524 2216 42-43 -0.5923849804935557 2216 44-45 -0.5959562239382379 2216 46-47 -0.524761407820165 2216 48-49 -0.7296887387257485 2216 50-51 -0.7627746565018043 2216 52-53 -0.7254514742182749 2216 54-55 -0.9202657191464851 2216 56-57 -0.803228366587696 2216 58-59 -0.5866920081744382 2216 60-61 -0.4802077969670364 2216 62-63 -0.549965327524184 2216 64-65 -0.6881579807660856 2216 66-67 -0.8908887615384167 2216 68-69 -0.6740792384654455 2216 70-71 -0.5906907761469391 2216 72-73 -0.44197988556522105 2216 74-75 -0.39634736793854586 2216 76-77 -0.5479131593172042 2216 78-79 -0.6326233410901843 2216 80-81 -0.6268337353245315 2216 82-83 -0.5807626727029707 2216 84-85 -0.7159584596379034 2216 86-87 -0.7739577670038997 2216 88-89 -0.7922645089789953 2216 90-91 -0.7851785916679788 2216 92-93 -0.9881231322343496 2216 94-95 -0.953728102461266 2216 96-97 -0.9257623357376623 2216 98-99 -1.1152169379449504 2216 100-101 -1.0233446016168664 2216 102-103 -1.0264688268725557 2216 104-105 -0.9929270829364789 2216 106-107 -0.985892907233076 2216 108-109 -0.9938470794849792 2216 110-111 -1.0413335363572855 2216 112-113 -0.9136808599935726 2216 114-115 -0.976159833422777 2216 116-117 -1.0759213120747901 2216 118-119 -0.8488580091730071 2216 120-121 -0.9588342665153995 2216 122-123 -1.0151131840036953 2216 124-125 -1.4495510348054736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 18.0 4 43.0 5 150.0 6 446.0 7 911.0 8 1311.0 9 2482.0 10 6310.0 11 19800.0 12 27822.0 13 8025.0 14 6059.0 15 7776.0 16 4577.0 17 2722.0 18 2273.0 19 2259.0 20 2169.0 21 2330.0 22 2440.0 23 2663.0 24 3201.0 25 3931.0 26 4999.0 27 6188.0 28 8044.0 29 10158.0 30 13049.0 31 16988.0 32 23161.0 33 31545.0 34 53353.0 35 79980.0 36 160963.0 37 270494.0 38 46464.0 39 295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 74.3991881212348 8.999042731597774 6.19465417179489 10.407114975372531 2 10.329220717814405 34.43165345338803 44.862263091679765 10.376862737117802 3 41.61615218505059 20.04347597090749 18.694188679968015 19.64618316407391 4 14.945818262876701 16.33721390101317 49.661079512088726 19.05588832402141 5 16.842865302339614 31.258703661794414 29.618014768834332 22.280416267031637 6 29.222907459456355 38.85354969379791 15.899166383367888 16.02437646337785 7 44.67898166635544 22.207219500983953 18.518225912253232 14.59557292040737 8 44.15683908623851 21.081536597861632 18.47489358442143 16.28673073147842 9 54.58939626815289 8.44812809131869 17.56048570511992 19.401989935408498 10-11 36.32308439988317 24.18991290441511 24.973007072027425 14.513995623674294 12-13 26.88752986578949 27.8927919904621 28.69043001948758 16.529248124260835 14-15 23.208112482104468 27.578872018807665 28.62620959439984 20.58680590468803 16-17 21.2700681346989 30.657262833311787 30.276608682745117 17.796060349244197 18-19 21.087402023452125 24.176566068632663 33.37050097916697 21.365530928748246 20-21 20.74032444182695 24.737552130466216 31.862428238313438 22.659695189393396 22-23 23.050105098850377 20.274561768916595 32.703219041326115 23.972114090906913 24-25 20.263010471580216 18.635833680470768 41.15643449157533 19.94472135637368 26-27 15.683010854628417 21.206745478834193 38.199900886277774 24.91034278025961 28-29 18.953105323891194 22.05477828691268 30.689702227904103 28.30241416129202 30-31 18.805272658498165 25.692239922241217 30.066889792244233 25.43559762701639 32-33 26.43559283891387 23.366239567921628 27.81821729366869 22.379950299495814 34-35 24.608393064912306 18.0115249627725 33.23846905209935 24.141612920215845 36-37 21.74187578704435 25.17967354716999 33.874508620978595 19.20394204480706 38-39 21.038683080282112 28.876387951218813 27.894527677626634 22.19040129087244 40-41 20.691785052501544 28.423433452557084 27.03009561840738 23.85468587653399 42-43 20.887019932870803 23.549564043265296 24.414235507610687 31.149180516253217 44-45 22.310702366759077 22.658617866325752 25.336543756074903 29.694136010840268 46-47 24.494855183839196 21.058733259596554 26.05912827805469 28.38728327850956 48-49 18.35064232395344 21.794604766077253 28.10957333218419 31.74517957778512 50-51 23.771492595199447 22.625759512762684 27.70821063820619 25.89453725383168 52-53 26.092944252122326 19.90755371054005 29.97286342895174 24.026638608385884 54-55 21.12660461285797 21.273000847494146 30.985966071505523 26.61442846814236 56-57 20.74307760077759 24.274303211380364 28.854602084739838 26.128017103102213 58-59 21.466320486854265 22.57027737477915 33.46931544498231 22.49408669338428 60-61 23.582302694265287 26.78739867178036 29.51984907900848 20.11044955494587 62-63 24.81260563751191 22.13204629137519 29.268653250403396 23.7866948207095 64-65 28.89715634591168 18.63343962920934 30.739857601830973 21.72954642304801 66-67 28.75482999841993 22.270362603004056 26.791289005080177 22.18351839349584 68-69 21.350328703238194 27.29673307764866 26.69504814437087 24.65789007474228 70-71 21.093566705450296 28.244597823328593 24.263290575577802 26.398544895643305 72-73 21.177298648318658 28.07551795299041 25.604258538383824 25.142924860307108 74-75 19.81418571134445 26.43625120301076 28.499205174981206 25.250357910663585 76-77 21.238825765737296 26.704205390445818 25.776989336895685 26.2799795069212 78-79 21.01552063432782 26.92870754748601 24.468221363555838 27.587550454630332 80-81 19.08465844070653 21.01929126506457 29.14194808739245 30.754102206836453 82-83 20.066518714298713 18.77385073569195 30.111239591862144 31.0483909581472 84-85 20.928018060722717 19.86386227501903 31.103154880752303 28.10496478350594 86-87 22.34978525360185 21.997799866890748 25.540995733800653 30.111419145706748 88-89 19.802514711445003 23.282447773771732 27.276982154741898 29.63805536004137 90-91 18.797791248306208 24.369167492614352 26.76914403091199 30.063897228167452 92-93 21.04694255713403 22.635036461400713 27.07019597703626 29.247825004428996 94-95 19.472434893775944 20.448429741777634 29.012130657741643 31.067004706704783 96-97 20.628222991510693 19.098424235459728 28.49076614428468 31.782586628744898 98-99 18.86296929389852 19.801796496066572 30.706340884171013 30.628893325863892 100-101 17.526490177207673 22.276108326031476 30.71585723793518 29.48154425882567 102-103 17.49309316211079 23.522212007603507 29.95556640858794 29.029128421697763 104-105 16.989205222862232 23.169029595261694 28.77757348540347 31.064191696472605 106-107 17.540864983208714 24.374568696444292 26.891077186054403 31.19348913429259 108-109 18.883498283465244 24.3470823697277 27.00364135196863 29.765777994838427 110-111 19.67296711715486 25.60744083601671 25.425729300087387 29.293862746741045 112-113 17.7952584362332 25.49403010176568 25.994509213855167 30.716202248145947 114-115 19.135501363123545 23.034962398139808 26.169398400162798 31.660137838573853 116-117 20.560283373814737 23.411858078464093 24.98123570232342 31.04662284539775 118-119 18.11690530898609 23.826903866538263 26.008291980169634 32.04789884430601 120-121 17.031442460429087 23.624160431123332 26.93491373583162 32.40948337261596 122-123 17.758571672257915 24.747944803727634 27.03486127612202 30.458622247892432 124-125 18.72996638963359 25.85040692850104 24.41523111168053 31.00439557018484 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27.0 1 56.0 2 287.5 3 714.0 4 953.5 5 851.0 6 716.5 7 726.0 8 857.0 9 1046.5 10 1215.5 11 1355.5 12 1408.5 13 1445.0 14 1515.0 15 1601.0 16 1728.0 17 1879.0 18 2004.5 19 2114.0 20 2224.0 21 2326.5 22 2403.0 23 2548.5 24 2692.0 25 2791.0 26 2964.5 27 3146.0 28 3321.5 29 3674.5 30 4077.0 31 4302.0 32 4736.0 33 5387.0 34 6109.5 35 6552.0 36 7174.5 37 8093.0 38 8573.5 39 9000.5 40 9331.5 41 9628.5 42 10002.5 43 10570.0 44 11323.5 45 12825.5 46 18389.0 47 32066.5 48 48661.0 49 64104.0 50 75706.0 51 79759.5 52 74596.0 53 60313.0 54 44759.5 55 34243.5 56 27426.0 57 21982.0 58 17510.0 59 12787.5 60 9224.5 61 7238.5 62 5656.0 63 4147.5 64 3074.5 65 2745.0 66 2476.0 67 2123.0 68 1763.5 69 1257.5 70 951.5 71 770.0 72 710.0 73 668.5 74 574.0 75 469.5 76 310.5 77 196.5 78 146.0 79 112.0 80 91.5 81 66.0 82 37.0 83 14.5 84 3.5 85 3.5 86 3.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21330996739302183 2 8.379179414989634E-4 3 0.0 4 0.11850553744056767 5 0.031960584340031885 6 9.576205045702438E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 5.985128153564024E-5 108-109 0.0 110-111 0.0016758358829979268 112-113 5.386615338207622E-4 114-115 7.780666599633231E-4 116-117 0.0049078050859225 118-119 0.002214497416818689 120-121 5.985128153564024E-4 122-123 0.00257360510603253 124-125 0.029327127952463717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 835404.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.27645580114512 #Duplication Level Percentage of deduplicated Percentage of total 1 83.23878494329054 36.85518382482628 2 8.806968986517505 7.798827461471963 3 2.6192231220931737 3.479097503960872 4 1.209279961605036 2.1417052308486335 5 0.7257446064381321 1.6066699494938708 6 0.5216715913232604 1.3858661493562427 7 0.3664957481255263 1.1358992955231237 8 0.2994438632269241 1.060665038007284 9 0.23171700686673763 0.9233647031617876 >10 1.6644832130256397 14.822617504076089 >50 0.17839663197438982 5.544960069496613 >100 0.12019431144961515 9.842532742168817 >500 0.009204068894776169 2.8020839831730777 >1k 0.006767697716747184 5.529015829403323 >5k 0.0016242474520193242 5.0715107150320495 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 9201 1.1013832828188517 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 8798 1.0531431499011257 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 7568 0.9059089973234506 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6496 0.777587849711038 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 5195 0.621854815155302 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 5054 0.6049767537622516 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 3840 0.4596578421937171 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3074 0.3679656788811162 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2929 0.35060880723578053 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 2844 0.3404340893747217 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2787 0.3336110432796587 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2599 0.31110696142225797 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 2328 0.2786675668299409 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 2146 0.2568817003509679 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1946 0.23294118773671182 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1697 0.20313524953196296 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1690 0.202297331590464 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1672 0.20014268545518096 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1662 0.19894565982446819 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1601 0.19164380347712007 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1492 0.1785962241023505 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1395 0.16698507548443625 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1343 0.16076054220472968 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1305 0.15621184480802103 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1262 0.15106463459595595 No Hit GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG 1175 0.14065051160875455 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1094 0.13095460399998085 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1084 0.12975757836926805 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1084 0.12975757836926805 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1046 0.1252088809725594 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1034 0.12377245021570403 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 994 0.11898434769285281 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 987 0.11814642975135385 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 923 0.1104854657147919 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 922 0.1103657631517206 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 914 0.10940814264715035 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 890 0.10653528113343963 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 859 0.10282450167822994 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1970256307128048E-4 0.0 10-11 0.0 0.0 0.0 1.1970256307128048E-4 0.0 12-13 0.0 0.0 0.0 1.1970256307128048E-4 0.0 14-15 0.0 0.0 0.0 2.3940512614256096E-4 0.0 16-17 0.0 0.0 0.0 2.3940512614256096E-4 0.0 18-19 0.0 0.0 0.0 4.788102522851219E-4 0.0 20-21 0.0 0.0 0.0 0.001197025630712805 0.0 22-23 0.0 0.0 0.0 0.0035910768921384147 0.0 24-25 0.0 0.0 0.0 0.007421558910419389 0.0 26-27 0.0 0.0 0.0 0.012748322967091372 0.0 28-29 0.0 0.0 0.0 0.026334563875681706 0.0 30-31 0.0 0.0 0.0 0.07026540452284163 0.0 32-33 0.0 0.0 0.0 0.1557330345557359 0.0 34-35 0.0 0.0 0.0 0.2626274233783894 0.0 36-37 0.0 0.0 0.0 0.39902849399811346 0.0 38-39 0.0 0.0 0.0 0.6195206151754122 0.0 40-41 0.0 5.985128153564024E-5 0.0 0.9466677200492217 0.0 42-43 0.0 1.1970256307128048E-4 0.0 1.6773321650363178 0.0 44-45 0.0 1.1970256307128048E-4 0.0 2.7230537560270243 0.0 46-47 0.0 1.1970256307128048E-4 0.0 3.720894321789218 0.0 48-49 0.0 1.7955384460692072E-4 0.0 4.058694954776372 0.0 50-51 0.0 2.3940512614256096E-4 0.0 4.516258002116341 0.0 52-53 0.0 2.3940512614256096E-4 0.0 4.87512628620404 0.0 54-55 0.0 2.3940512614256096E-4 0.0 5.250393821432505 0.0 56-57 0.0 2.3940512614256096E-4 0.0 5.93425456425873 0.0 58-59 0.0 2.3940512614256096E-4 0.0 6.647861394008169 0.0 60-61 0.0 2.3940512614256096E-4 0.0 7.718660671962308 0.0 62-63 0.0 2.3940512614256096E-4 0.0 9.071000378260099 0.0 64-65 0.0 2.3940512614256096E-4 0.0 10.19285279936414 0.0 66-67 0.0 2.3940512614256096E-4 0.0 11.042561443325624 0.0 68-69 0.0 2.3940512614256096E-4 0.0 11.725285011802672 0.0 70-71 0.0 2.3940512614256096E-4 0.0 12.530524153583176 0.0 72-73 0.0 2.3940512614256096E-4 0.0 13.346416823477025 0.0 74-75 0.0 2.3940512614256096E-4 0.0 14.258789759206323 0.0 76-77 0.0 2.3940512614256096E-4 0.0 15.381420246970329 0.0 78-79 0.0 2.3940512614256096E-4 0.0 16.755785224873236 0.0 80-81 0.0 2.3940512614256096E-4 0.0 18.736742941139855 0.0 82-83 0.0 2.3940512614256096E-4 0.0 20.143248057227403 0.0 84-85 0.0 2.3940512614256096E-4 0.0 20.977574921834226 0.0 86-87 0.0 2.3940512614256096E-4 0.0 21.78131778157634 0.0 88-89 0.0 2.3940512614256096E-4 0.0 22.90783860264016 0.0 90-91 0.0 2.3940512614256096E-4 0.0 24.46307415334377 0.0 92-93 0.0 2.3940512614256096E-4 0.0 26.79248603071089 0.0 94-95 0.0 2.3940512614256096E-4 0.0 30.091249263829237 0.0 96-97 0.0 2.3940512614256096E-4 0.0 33.5702247056514 0.0 98-99 0.0 2.3940512614256096E-4 0.0 36.35516468678627 0.0 100-101 0.0 2.3940512614256096E-4 0.0 38.2075019990328 0.0 102-103 0.0 2.3940512614256096E-4 0.0 39.68415281708012 0.0 104-105 0.0 2.3940512614256096E-4 0.0 41.28361846483857 0.0 106-107 0.0 2.3940512614256096E-4 0.0 42.79175105697363 0.0 108-109 0.0 2.3940512614256096E-4 0.0 44.005116087545666 0.0 110-111 0.0 2.3940512614256096E-4 0.0 45.05544622721462 0.0 112-113 0.0 2.3940512614256096E-4 0.0 47.18974292677555 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCGAT 65 0.0 91.657135 2 GAGTAGT 1755 0.0 88.44796 1 AGTAGTA 1350 0.0 84.731926 2 GAGTCGG 550 0.0 83.58332 1 GAGTATG 575 0.0 83.06417 1 GAGTCCG 540 0.0 80.708755 1 GAGTTAG 1095 0.0 80.69362 1 AGTACGT 105 0.0 79.43618 2 GTTGCGT 45 4.2628017E-8 79.43618 3 GAGTCGA 350 0.0 78.46597 1 AGTTGCG 85 0.0 77.099815 2 GAGTAAG 985 0.0 76.97666 1 GAGTCAG 3850 0.0 76.45005 1 AGTCGTA 400 0.0 75.960846 2 GAGTACG 645 0.0 75.90069 1 GTACGTT 55 1.9717845E-9 75.82544 3 GAGTTGT 835 0.0 75.78984 1 GAGTAAT 475 0.0 75.41352 1 GAGTCGT 610 0.0 75.36201 1 AGTCCGT 230 0.0 75.118996 2 >>END_MODULE