FastQCFastQC Report
Thu 26 May 2016
SRR935976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473993
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT11240.23713430367115124No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA10790.22764049258111405No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT9800.20675410818303225No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6770.14282911351011512No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6610.13945353623365744No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC6500.1371328268560928No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG401.7733853E-459.4832275
TATATGG1300.054.919182
GTATATG1450.049.3159451
GATTGTG856.204937E-848.9861879
GGAAACA4450.048.207721
GTGCTAG1004.083631E-947.6720771
ACTAGGG1251.2732926E-1147.5966263
GTAGGGT505.331277E-447.5966224
GAAAACA4950.045.7459341
GTACTAG1352.7284841E-1144.1408121
TGCTAGG1109.616997E-943.2696572
GAGAAAA4050.042.669451
CTAGGGC705.4493845E-542.4969864
GTAACAA758.1087244E-539.726731
GTTAGAG758.184782E-539.6638573
AGGGTAA905.159047E-639.6554838
ATATGGG1850.038.5918543
GGGATTG1401.893568E-938.2392167
TCTATGG801.1969072E-437.184862
ATTTAGC401.19930046E-437.17701718-19
Produced by FastQC (version 0.11.5)