##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935976_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 473993 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.414974482745524 31.0 30.0 34.0 12.0 34.0 2 28.341340483931198 31.0 30.0 34.0 10.0 34.0 3 28.346467141919817 31.0 30.0 34.0 7.0 34.0 4 31.465207292090813 37.0 33.0 37.0 7.0 37.0 5 31.631566288953636 37.0 33.0 37.0 10.0 37.0 6 31.790992693984933 37.0 35.0 37.0 8.0 37.0 7 31.782290033819066 37.0 35.0 37.0 8.0 37.0 8 31.537206245661856 37.0 35.0 37.0 8.0 37.0 9 32.744622810885396 39.0 35.0 39.0 7.0 39.0 10-11 32.76906515497065 39.0 34.0 39.0 7.0 39.0 12-13 32.74505003238444 39.0 34.0 39.0 7.0 39.0 14-15 33.709340644271116 40.0 34.0 41.0 7.0 41.0 16-17 33.79636724592979 39.5 34.0 41.0 8.0 41.0 18-19 33.95819558516687 40.0 33.5 41.0 8.0 41.0 20-21 34.12838902684217 40.0 34.0 41.0 8.0 41.0 22-23 33.8991820554312 40.0 33.0 41.0 4.5 41.0 24-25 33.618097735620566 39.0 33.0 41.0 2.0 41.0 26-27 33.4061357446207 39.0 33.0 41.0 2.0 41.0 28-29 33.159994978828806 39.0 33.0 41.0 2.0 41.0 30-31 32.944629983987106 39.0 33.0 41.0 2.0 41.0 32-33 32.61777811064721 39.0 32.0 41.0 2.0 41.0 34-35 32.361730025548894 39.0 32.0 41.0 2.0 41.0 36-37 32.20771825744262 39.0 32.0 41.0 2.0 41.0 38-39 32.069026335831964 39.0 32.0 41.0 2.0 41.0 40-41 31.82207754122951 38.0 32.0 41.0 2.0 41.0 42-43 31.556175935087648 38.0 31.0 41.0 2.0 41.0 44-45 31.216350241459264 37.5 31.0 40.0 2.0 41.0 46-47 30.845396451002443 37.0 31.0 40.0 2.0 41.0 48-49 30.45126932254274 36.0 30.5 39.5 2.0 41.0 50-51 30.051623125236027 35.5 30.0 39.0 2.0 41.0 52-53 29.68945005516959 35.0 30.0 39.0 2.0 41.0 54-55 29.299408430082302 35.0 29.5 37.5 2.0 40.0 56-57 28.945737595280946 35.0 29.0 37.0 2.0 39.5 58-59 28.608010033903454 35.0 29.0 36.0 2.0 39.0 60-61 28.29719742696622 35.0 28.5 36.0 2.0 39.0 62-63 28.00869949556217 35.0 28.0 35.0 2.0 37.5 64-65 27.79004225800803 35.0 28.0 35.0 2.0 37.0 66-67 27.64516775564196 35.0 28.5 35.0 2.0 36.0 68-69 27.539188342443875 35.0 28.5 35.0 2.0 36.0 70-71 27.336998647659357 35.0 27.5 35.0 2.0 35.0 72-73 27.145356576995862 35.0 26.0 35.0 2.0 35.0 74-75 26.928432487399604 34.0 25.5 35.0 2.0 35.0 76-77 26.69705248811691 34.0 25.0 35.0 2.0 35.0 78-79 26.484412217058058 34.0 24.5 35.0 2.0 35.0 80-81 26.19985632699217 34.0 23.5 35.0 2.0 35.0 82-83 25.875016086735457 34.0 19.5 35.0 2.0 35.0 84-85 25.576582354591736 34.0 18.0 35.0 2.0 35.0 86-87 25.289452586852548 34.0 12.5 35.0 2.0 35.0 88-89 24.91613272769851 33.5 6.0 35.0 2.0 35.0 90-91 24.502932532758923 33.0 2.0 35.0 2.0 35.0 92-93 24.058401706354314 33.0 2.0 35.0 2.0 35.0 94-95 23.657619416320493 33.0 2.0 35.0 2.0 35.0 96-97 23.25740675495208 32.0 2.0 35.0 2.0 35.0 98-99 22.80592856856536 31.5 2.0 35.0 2.0 35.0 100-101 22.408341473397286 31.0 2.0 35.0 2.0 35.0 102-103 21.59031884437112 29.5 2.0 34.0 2.0 35.0 104-105 21.872855717278526 30.5 2.0 35.0 2.0 35.0 106-107 21.720384056304628 31.0 2.0 35.0 2.0 35.0 108-109 21.422047372007604 30.0 2.0 35.0 2.0 35.0 110-111 21.002760589291405 29.5 2.0 35.0 2.0 35.0 112-113 20.541869816642862 29.0 2.0 35.0 2.0 35.0 114-115 19.979098847451333 29.0 2.0 35.0 2.0 35.0 116-117 19.311612196804596 27.0 2.0 34.5 2.0 35.0 118-119 18.499928268982877 25.0 2.0 34.0 2.0 35.0 120-121 17.6302624722306 20.5 2.0 34.0 2.0 35.0 122-123 16.73941703780436 2.0 2.0 34.0 2.0 35.0 124-125 14.447177489962932 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4778107805313354 1101 2 0.5346573211586616 1101 3 0.4795236306093571 1101 4 0.44983045873480165 1101 5 0.5545849840128412 1101 6 0.507534047043098 1101 7 0.4758427864798911 1101 8 0.17226461278010774 1101 9 0.3235166913316547 1101 10-11 0.4886596849844196 1101 12-13 0.25580065208370684 1101 14-15 0.3935804854023104 1101 16-17 0.5581986602547531 1101 18-19 0.5470423766644785 1101 20-21 0.2230805904254467 1101 22-23 0.46832939437818766 1101 24-25 0.772181869340379 1101 26-27 0.7965667550663369 1101 28-29 0.8648180901447802 1101 30-31 0.5884848952142931 1101 32-33 0.5065505579987217 1101 34-35 0.12388999887072316 1101 36-37 0.42872497474411375 1101 38-39 0.4889277939243897 1101 40-41 0.4549355559075714 1101 42-43 0.31104027799666767 1101 44-45 0.45459252229325386 1101 46-47 0.6258565421903661 1101 48-49 0.5844218818401608 1101 50-51 0.4249866558156512 1101 52-53 0.5583648502583962 1101 54-55 0.422347413864518 1101 56-57 0.27618597152492796 1101 58-59 0.21088387050829738 1101 60-61 0.4090270213771987 1101 62-63 0.4850014712654449 1101 64-65 0.43042899782471267 1101 66-67 0.08616340604836736 1101 68-69 0.11042318622530445 1101 70-71 0.03748907173946492 1101 72-73 0.06461743051461255 1101 74-75 0.1470011894926806 1101 76-77 0.3436144305333606 1101 78-79 0.5597034819256308 1101 80-81 0.6640447453215721 1101 82-83 0.7110364026360223 1101 84-85 0.6092111107264095 1101 86-87 0.3807302014796896 1101 88-89 0.3136482166276764 1101 90-91 0.31027760962933826 1101 92-93 0.28862129050482466 1101 94-95 0.40992439640940503 1101 96-97 0.3366115868149464 1101 98-99 0.25956200768829163 1101 100-101 0.5232362962842139 1101 102-103 0.5417439660440451 1101 104-105 0.3288436662916254 1101 106-107 0.6414391614292505 1101 108-109 0.5312848577662983 1101 110-111 0.8364218277226847 1101 112-113 0.6753627237209514 1101 114-115 0.5649469766634034 1101 116-117 0.6926410580200333 1101 118-119 0.5725063224573468 1101 120-121 -0.09020015506882473 1101 122-123 -0.021738747707235717 1101 124-125 -0.018794860364447885 1102 1 0.6867674734447213 1102 2 0.7577872424185053 1102 3 0.6667115507169044 1102 4 0.879470846674117 1102 5 0.7714372982052708 1102 6 0.8959102713565237 1102 7 0.8056248106780863 1102 8 0.6337683577388162 1102 9 0.6685954314891376 1102 10-11 0.7262100978901245 1102 12-13 0.7854074314192161 1102 14-15 0.9887096384654939 1102 16-17 0.8139145235157486 1102 18-19 0.7974528817537774 1102 20-21 0.9777197780574838 1102 22-23 0.8913632910557396 1102 24-25 0.8602208073084938 1102 26-27 0.8010186947629023 1102 28-29 0.8726488948307818 1102 30-31 1.0669801900097582 1102 32-33 1.1787682457267792 1102 34-35 1.3369529429844107 1102 36-37 1.1879397337224162 1102 38-39 1.0235794647521246 1102 40-41 1.1401777779164028 1102 42-43 1.004432586427626 1102 44-45 1.024814099016897 1102 46-47 1.01533344827833 1102 48-49 1.0715281810527593 1102 50-51 1.0732822194799496 1102 52-53 0.9964674045122841 1102 54-55 1.029676490108038 1102 56-57 1.1004381800903218 1102 58-59 0.8785319884264844 1102 60-61 0.831798050760721 1102 62-63 0.87428272726698 1102 64-65 0.8100321762383871 1102 66-67 0.7882819483952126 1102 68-69 0.8718741176637508 1102 70-71 0.899685393997963 1102 72-73 0.9664815077183739 1102 74-75 0.8577451399439902 1102 76-77 0.8956333473760729 1102 78-79 1.0308793029358085 1102 80-81 1.1155631340290775 1102 82-83 1.0025982424670197 1102 84-85 1.0268232194262374 1102 86-87 0.8619573956413973 1102 88-89 0.9491341010140779 1102 90-91 0.8118872216900215 1102 92-93 0.9243892471017183 1102 94-95 1.1362651396396721 1102 96-97 1.005339981283928 1102 98-99 1.0058962692592566 1102 100-101 1.3255780957848842 1102 102-103 0.9386579760306049 1102 104-105 0.9753663761053346 1102 106-107 0.9641213680741529 1102 108-109 1.2024654800055927 1102 110-111 0.7579757455256413 1102 112-113 0.6032110528932186 1102 114-115 0.7294622925842766 1102 116-117 0.4616407219339962 1102 118-119 0.5401604419119259 1102 120-121 0.33473382803084917 1102 122-123 0.29721650483741513 1102 124-125 0.012091446530115846 1103 1 0.679061993992665 1103 2 0.862714944092783 1103 3 0.5236996154605684 1103 4 0.6796318705446254 1103 5 0.7206799755635558 1103 6 0.6316223031343746 1103 7 0.7865200477126457 1103 8 0.7644529180090771 1103 9 0.8552392671055742 1103 10-11 1.1091081563227618 1103 12-13 0.9582203838638108 1103 14-15 1.0699524457565772 1103 16-17 0.8846095712196487 1103 18-19 0.7005485308609565 1103 20-21 1.008900171197034 1103 22-23 1.108738555561807 1103 24-25 1.2545749948572222 1103 26-27 1.037506843034798 1103 28-29 1.0188743468113302 1103 30-31 1.1226331951699464 1103 32-33 1.0498168962817829 1103 34-35 1.019405285487295 1103 36-37 1.0025082175921867 1103 38-39 0.929175160263874 1103 40-41 0.9104995008254129 1103 42-43 0.660466805160226 1103 44-45 0.567184955088468 1103 46-47 0.5217709227558132 1103 48-49 0.5860829755733086 1103 50-51 0.3197972359999781 1103 52-53 0.6089335291735303 1103 54-55 0.6837791184630859 1103 56-57 0.6050333929945033 1103 58-59 0.4775721098208514 1103 60-61 0.5120879401322185 1103 62-63 0.2048758705271787 1103 64-65 0.20729593055401097 1103 66-67 0.4582352189895609 1103 68-69 0.5152039242494872 1103 70-71 0.5919731858827326 1103 72-73 0.6285227985079942 1103 74-75 0.7082794886093957 1103 76-77 0.6056813297052095 1103 78-79 0.29888659773477855 1103 80-81 0.369401952383015 1103 82-83 0.48847501048127384 1103 84-85 0.6439634447668467 1103 86-87 0.3800830221031397 1103 88-89 0.4372498897062087 1103 90-91 0.31761470619089494 1103 92-93 0.43973407948839593 1103 94-95 0.3977514317286186 1103 96-97 -0.17530490307587598 1103 98-99 0.10297695753189018 1103 100-101 0.06816612526107235 1103 102-103 0.192919076004614 1103 104-105 0.12946088366000197 1103 106-107 0.2874092713298957 1103 108-109 0.6742491790069636 1103 110-111 0.634447930624205 1103 112-113 0.6442477774727955 1103 114-115 0.6554945623247868 1103 116-117 0.5314250798803144 1103 118-119 0.454233092912407 1103 120-121 0.5358524102783164 1103 122-123 0.2988499458293603 1103 124-125 0.42244245072508946 1104 1 -0.1809273317500839 1104 2 -0.3162567691255056 1104 3 -0.43354405411187 1104 4 -0.5619226175852035 1104 5 -0.5054166066974126 1104 6 -0.736955846265694 1104 7 -0.7130154244255671 1104 8 -0.591612629837293 1104 9 -0.8579197200260147 1104 10-11 -0.792016594117058 1104 12-13 -0.6396862756866852 1104 14-15 -0.6073489438735749 1104 16-17 -0.3556007511824575 1104 18-19 -0.462500748626681 1104 20-21 -0.29722368972134916 1104 22-23 -0.17476181013033454 1104 24-25 -0.3672597913611213 1104 26-27 -0.09683066116571837 1104 28-29 -0.12432872104927384 1104 30-31 -0.3574514082023299 1104 32-33 -0.40662620399693594 1104 34-35 -0.25361849438580464 1104 36-37 -0.4233946466668428 1104 38-39 -0.5455031294829098 1104 40-41 -0.526127038540853 1104 42-43 -0.6405373657069617 1104 44-45 -0.7479230723483248 1104 46-47 -0.5842501490186827 1104 48-49 -0.3522633052387256 1104 50-51 -0.3848223453319335 1104 52-53 -0.5259871057471024 1104 54-55 -0.46804242376941474 1104 56-57 -0.5515026128565701 1104 58-59 -0.3854415888092859 1104 60-61 -0.4709913854128658 1104 62-63 -0.4960077207673699 1104 64-65 -0.45102900174352456 1104 66-67 -0.5040787227764376 1104 68-69 -0.5398080348720597 1104 70-71 -0.4187504892245002 1104 72-73 -0.5557959269066224 1104 74-75 -0.4025153482128374 1104 76-77 -0.36619002572778925 1104 78-79 -0.616322242131595 1104 80-81 -0.645522240623368 1104 82-83 -0.5065239023258563 1104 84-85 -0.4391771589217015 1104 86-87 -0.5173848068715401 1104 88-89 -0.4489803184418051 1104 90-91 -0.33641087249221613 1104 92-93 -0.30213668293031404 1104 94-95 -0.22308511376546036 1104 96-97 -0.39624226099953574 1104 98-99 -0.4696672931945507 1104 100-101 -0.46219620647112336 1104 102-103 -0.19866802791125338 1104 104-105 -0.5457254216632919 1104 106-107 -0.5354375926128583 1104 108-109 -0.5311709716186641 1104 110-111 -0.5643008092204269 1104 112-113 -0.5265699892353872 1104 114-115 -0.3009584215254577 1104 116-117 -0.6597330770312659 1104 118-119 -0.8001107367738847 1104 120-121 -0.687644792670941 1104 122-123 -0.6490932829357874 1104 124-125 -0.132609042682569 1105 1 0.702171156164269 1105 2 0.5338141545998099 1105 3 0.6823743365940551 1105 4 0.6135921414989625 1105 5 0.80914544440855 1105 6 0.7417179822664899 1105 7 0.7467133894108962 1105 8 0.9354508009088534 1105 9 0.744020360667605 1105 10-11 0.9772033266609341 1105 12-13 1.2066616634374583 1105 14-15 1.135064057038008 1105 16-17 1.3264248876420552 1105 18-19 1.1419175746301704 1105 20-21 1.0113967109670412 1105 22-23 1.1622945078041624 1105 24-25 1.0514885990586507 1105 26-27 0.969766103731331 1105 28-29 0.6771901095063839 1105 30-31 0.7613161949845306 1105 32-33 0.9485248614940716 1105 34-35 0.9142326189327292 1105 36-37 1.0046177721387792 1105 38-39 0.9418777615806988 1105 40-41 1.0850341483993695 1105 42-43 0.941010611025149 1105 44-45 0.8546761697693306 1105 46-47 1.0266238683709155 1105 48-49 0.9930933368601238 1105 50-51 0.8764242771044906 1105 52-53 1.0141900604923855 1105 54-55 1.0718455290080584 1105 56-57 1.0288415000654503 1105 58-59 1.1334441524560575 1105 60-61 1.0775811468707204 1105 62-63 1.115054336470326 1105 64-65 0.9355482233606125 1105 66-67 1.0389405022541212 1105 68-69 1.2406901889689408 1105 70-71 0.9572689115824318 1105 72-73 1.0543800947424877 1105 74-75 0.7508802017832785 1105 76-77 0.9212370577332898 1105 78-79 0.9324935156076783 1105 80-81 0.8380917217441315 1105 82-83 0.7994138646600675 1105 84-85 0.5240702483766988 1105 86-87 0.5098707515454919 1105 88-89 0.7346916910092389 1105 90-91 0.6201703178000209 1105 92-93 0.7630262781250003 1105 94-95 0.8336089004356388 1105 96-97 0.8868067642591235 1105 98-99 0.8920161131064184 1105 100-101 0.9601627534232478 1105 102-103 0.5540646816203214 1105 104-105 0.6517184627494323 1105 106-107 0.8883531272118574 1105 108-109 0.7939591233414589 1105 110-111 0.573997389276073 1105 112-113 0.6450756455830557 1105 114-115 0.4039099710199885 1105 116-117 0.3574143175948805 1105 118-119 0.4527476733083198 1105 120-121 0.5673760756643738 1105 122-123 0.43271018272037054 1105 124-125 0.15094482868999926 1106 1 0.5563749699086529 1106 2 0.5920340599574025 1106 3 0.7078700873022719 1106 4 0.09445730612145198 1106 5 0.7237348798798209 1106 6 0.752350227198793 1106 7 0.799764424987945 1106 8 0.8089417113111068 1106 9 0.952718486651797 1106 10-11 0.9825965814862201 1106 12-13 0.890702477474342 1106 14-15 1.1431689650601697 1106 16-17 1.1417489837910395 1106 18-19 0.8250708656776027 1106 20-21 0.7623894144462113 1106 22-23 1.0272798226043633 1106 24-25 0.9424354638786809 1106 26-27 0.7677915153376489 1106 28-29 0.9279193609017824 1106 30-31 0.842461440225371 1106 32-33 0.9266142629068952 1106 34-35 0.9337430989163806 1106 36-37 0.8502398596409364 1106 38-39 1.0480419393096838 1106 40-41 0.9134026287115837 1106 42-43 0.9057601211845849 1106 44-45 0.8299347134302515 1106 46-47 0.9901572917253887 1106 48-49 0.9720762715760962 1106 50-51 0.9480752301398994 1106 52-53 0.8400352107702638 1106 54-55 0.7871259502108394 1106 56-57 1.0777674570075604 1106 58-59 0.9280897643260282 1106 60-61 0.8558055799979627 1106 62-63 0.9466794050880729 1106 64-65 0.8915266823963179 1106 66-67 1.0133515916835805 1106 68-69 1.0409714716500211 1106 70-71 1.009992385062926 1106 72-73 0.9891371378284255 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-4.617001554415424 2216 78-79 -4.963549253075417 2216 80-81 -4.971625242832566 2216 82-83 -4.857781101370666 2216 84-85 -4.64302218312071 2216 86-87 -4.68437327562992 2216 88-89 -4.677376799050677 2216 90-91 -4.587102363891674 2216 92-93 -4.200250565114992 2216 94-95 -4.038922373850507 2216 96-97 -4.034101255495695 2216 98-99 -3.8713835710170237 2216 100-101 -3.856033441016322 2216 102-103 -3.851622349892125 2216 104-105 -4.535869594108348 2216 106-107 -4.06315982122004 2216 108-109 -4.190511532729136 2216 110-111 -4.4024535119748975 2216 112-113 -4.686988331954613 2216 114-115 -4.357601974629189 2216 116-117 -4.13162902768828 2216 118-119 -3.6521609481161335 2216 120-121 -3.4976142725519246 2216 122-123 -2.8809401970835857 2216 124-125 -2.7572103017971 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38558.0 3 3705.0 4 2246.0 5 4425.0 6 10745.0 7 7841.0 8 4791.0 9 4943.0 10 3501.0 11 2507.0 12 2086.0 13 1742.0 14 1853.0 15 1947.0 16 1875.0 17 2063.0 18 2418.0 19 2818.0 20 3499.0 21 4314.0 22 5401.0 23 6138.0 24 7210.0 25 7838.0 26 8220.0 27 8980.0 28 10276.0 29 12709.0 30 16365.0 31 21580.0 32 27610.0 33 33996.0 34 43306.0 35 56044.0 36 79519.0 37 19967.0 38 957.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.06794003613045 18.57654478802413 10.951540826351987 13.403974349493433 2 19.69560456023447 25.199663236432922 33.82279399818114 21.28193820515147 3 30.654972265533516 24.82071234009693 24.36582067225224 20.158494722117315 4 16.50817095171279 23.856647008556024 27.30923016869402 32.32595187103717 5 16.04765581815343 35.74903688325717 24.540805347038493 23.6625019515509 6 31.435696307751382 37.398864540193635 8.57396628220248 22.59147286985251 7 28.762028131217125 42.108849708750974 11.171894943596214 17.957227216435687 8 34.77379365052575 30.665285490050465 9.873795338317946 24.68712552110584 9 35.8944119427924 19.484043013293444 13.841554622114671 30.77999042179948 10-11 29.70043861407236 29.45855740485619 13.8314278902853 27.00957609078615 12-13 32.41218752175665 30.554670638595926 16.2699660121563 20.763175827491125 14-15 36.14251687261204 23.78199678054317 20.037110252683057 20.038376094161727 16-17 30.506990609565964 24.784437744861208 21.32774112697867 23.380830518594156 18-19 29.389252583898916 25.075686771745577 21.4063287854462 24.12873185890931 20-21 38.64339768730762 22.901815005706837 17.21586605709367 21.238921249891877 22-23 34.3532499425097 26.000700432284862 16.527037319116527 23.11901230608891 24-25 27.74408060878536 28.12056296190028 17.895095497190887 26.240260932123473 26-27 24.90988667398755 27.73064211744427 20.628752084711273 26.730719123856904 28-29 26.461810474967745 26.157986405982804 22.575183270265224 24.80501984878423 30-31 27.477515490735094 27.619500709926097 19.847128544092424 25.055855255246385 32-33 24.486226589844154 27.67361543313931 21.319724130947083 26.52043384606946 34-35 23.09485583120426 28.212125495524194 21.211705658100435 27.48131301517111 36-37 24.13295133050488 29.922066359629785 19.017263968033284 26.92771834183205 38-39 24.986972381448673 26.939849322669325 20.492285751055395 27.580892544826614 40-41 23.286525328433118 27.238693398425717 21.924901844542006 27.549879428599155 42-43 23.245069020006625 27.344180188315015 23.48631730848346 25.924433483194896 44-45 23.78843147472642 26.534252615544958 24.20088482319359 25.476431086535033 46-47 21.350948220754315 26.77022656452732 23.743810562603247 28.135014652115114 48-49 20.67783701447068 27.139957763089328 24.859333365682616 27.32287185675738 50-51 21.704645427253144 27.158101490950287 23.59961012082457 27.537642960971997 52-53 21.202317333800288 26.39617040757986 23.1191177928788 29.282394465741056 54-55 20.035949897994275 25.888778948212316 22.915528288392444 31.159742865400965 56-57 20.473890354573868 25.189797707342855 23.813507204243116 30.522804733840164 58-59 21.85475312926562 23.414902751728402 23.786321738928635 30.944022380077342 60-61 21.746840143208864 23.183148274341605 23.63104518421158 31.438966398237948 62-63 20.921722472694746 22.718795425248896 24.062591641648716 32.296890460407646 64-65 20.513537291107138 22.11917204480292 24.519448845643446 32.8478418184465 66-67 21.3978069273248 22.8853832075402 23.73138815487587 31.985421710259125 68-69 20.961485512100587 22.46328575241395 24.404992357022522 32.170236378462945 70-71 19.941698590363494 21.683672123675795 26.150281747945048 32.224347538015664 72-73 20.68600313929349 20.512266029806412 27.031152424513493 31.77057840638661 74-75 20.74695012596159 19.616671660118914 27.837406161779416 31.79897205214008 76-77 20.664461990432336 19.301782201195167 28.40983770840256 31.623918099969938 78-79 21.325638383047764 19.78309691860913 29.197631598101648 29.693633100241463 80-81 22.179499756851154 20.745818209053155 28.634917018203993 28.439765015891705 82-83 21.436919954514096 20.731213330154667 28.70875730232303 29.12310941300821 84-85 21.395879799708382 20.432192678341202 28.72756162047988 29.444365901470537 86-87 22.014854916141775 20.230513867014636 28.514856498471996 29.239774718371592 88-89 22.639122613590203 19.92762300659939 28.339285620084087 29.09396875972632 90-91 23.36975440565578 20.24386436086609 27.801254448905365 28.58512678457277 92-93 23.51057062043776 21.038799910957596 27.25836961670621 28.192259851898427 94-95 22.82903412987287 21.65648573086459 26.695679696154457 28.81880044310809 96-97 22.533765572039375 22.39483170224056 25.832627411430977 29.23877531428909 98-99 22.55759736697751 23.30762901388244 25.495696020929152 28.639077598210893 100-101 22.022698767169384 24.900921010020244 25.41664864591168 27.659731576898693 102-103 21.633969278027312 26.308510885181846 25.455017268187508 26.60250256860333 104-105 20.759465897031802 27.865507994877543 25.40090423657833 25.974121871512327 106-107 19.775078693975377 28.97446770804324 25.12183852755766 26.12861507042372 108-109 19.720438909435373 30.383571065395483 24.911760300257598 24.98422972491155 110-111 19.60988875363138 32.5438350355385 23.98337106244185 23.862905148388265 112-113 19.33764076056858 33.92019831746617 23.422664099791664 23.31949682217358 114-115 18.775351318505923 34.83414897107273 23.39732195132758 22.993177759093772 116-117 18.342675946691195 35.4350169728245 23.200237134303674 23.02206994618064 118-119 18.26018386858748 36.12461430138276 23.103597883262765 22.511603946766993 120-121 18.607312920505585 36.05677840766441 22.640263469364935 22.695645202465066 122-123 19.05476741045809 36.36363636363637 22.65659285015022 21.925003375755324 124-125 19.034758710826264 38.25361561017288 21.738284492503364 20.973341186497493 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9.0 1 8.5 2 9.5 3 12.5 4 13.0 5 13.5 6 12.5 7 15.0 8 19.5 9 18.0 10 23.5 11 32.5 12 32.5 13 44.5 14 67.0 15 93.0 16 130.0 17 179.0 18 250.0 19 333.5 20 474.5 21 664.0 22 882.5 23 1142.5 24 1423.0 25 1744.5 26 2070.5 27 2374.0 28 2653.5 29 2913.0 30 3105.5 31 3197.0 32 3272.5 33 3350.0 34 3295.5 35 3124.0 36 2955.0 37 2786.0 38 2551.0 39 2269.5 40 2040.5 41 1911.5 42 1850.5 43 1743.5 44 1759.5 45 1991.5 46 2556.5 47 3683.0 48 5723.0 49 10057.5 50 17397.0 51 27349.5 52 38268.5 53 45581.5 54 46828.0 55 43835.5 56 37916.0 57 30499.0 58 23607.0 59 17673.0 60 13110.5 61 10000.0 62 7817.0 63 6127.5 64 4983.5 65 4350.0 66 3872.0 67 3409.5 68 2743.0 69 2083.0 70 1544.5 71 1103.0 72 786.0 73 576.5 74 400.0 75 270.0 76 196.0 77 159.5 78 133.5 79 109.0 80 83.5 81 64.0 82 55.0 83 47.0 84 36.0 85 26.5 86 21.0 87 15.5 88 13.0 89 8.0 90 5.5 91 3.5 92 3.5 93 3.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14936929448325187 2 0.014768150584502303 3 0.007173101712472547 4 0.012236467627159051 5 0.0023207093775646473 6 0.0 7 0.0 8 2.1097357977860432E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.001371328268560928 28-29 0.007278588502361849 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.164603696679065E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.001265841478671626 66-67 1.0548678988930216E-4 68-69 0.006012747023690223 70-71 0.01613947885306323 72-73 2.1097357977860432E-4 74-75 0.008227969611365567 76-77 1.0548678988930216E-4 78-79 5.274339494465108E-4 80-81 7.384075292251151E-4 82-83 0.0 84-85 0.017721780701402765 86-87 0.0017932754281181369 88-89 0.01803824107107067 90-91 0.0 92-93 0.012974875156384166 94-95 0.014346203424945095 96-97 0.005590799864133015 98-99 0.002742656537121856 100-101 5.274339494465108E-4 102-103 0.0 104-105 4.2194715955720864E-4 106-107 0.0010548678988930216 108-109 0.0 110-111 0.0 112-113 0.0011603546887823237 114-115 0.006012747023690223 116-117 0.0 118-119 0.07162553033483617 120-121 0.002109735797786043 122-123 0.005274339494465108 124-125 0.24209218279594846 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 473993.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.51249261446966 #Duplication Level Percentage of deduplicated Percentage of total 1 85.51965710353969 56.881255614911616 2 7.456730397685968 9.919314510083588 3 2.4648418622310193 4.918283284724289 4 1.2704004164208282 3.379899932584381 5 0.7882829780625813 2.6215332878249797 6 0.5175538840204308 2.0654279333099392 7 0.36226953753267627 1.6866814957712633 8 0.29156250573171677 1.5514039207309664 9 0.22189240182418996 1.3282755063784533 >10 1.0642809460213742 11.806883269226624 >50 0.027294068586147906 1.194150947436422 >100 0.013329661050480788 1.5553303858700602 >500 0.001269491528617217 0.6265228807359307 >1k 6.347457643086085E-4 0.46503703041147415 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1124 0.23713430367115124 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1079 0.22764049258111405 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 980 0.20675410818303225 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 677 0.14282911351011512 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 661 0.13945353623365744 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 650 0.1371328268560928 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.438943191144173E-4 0.0 0.0 0.0 0.0 3 8.438943191144173E-4 0.0 0.0 0.0 0.0 4 8.438943191144173E-4 0.0 0.0 0.0 0.0 5 0.0010548678988930216 0.0 0.0 0.0 0.0 6 0.0014768150584502304 0.0 0.0 0.0 0.0 7 0.001898762218007439 0.0 0.0 0.0 0.0 8 0.002109735797786043 0.0 0.0 0.0 0.0 9 0.0023207093775646477 0.0 0.0 0.0 0.0 10-11 0.004008498015793482 0.0 0.0 1.0548678988930216E-4 0.0 12-13 0.007700535661919057 0.0 0.0 6.32920739335813E-4 0.0 14-15 0.01265841478671626 0.0 0.0 0.0011603546887823239 0.0 16-17 0.013291335526052073 0.0 0.0 0.001265841478671626 0.0 18-19 0.014768150584502303 0.0 0.0 0.001898762218007439 0.0 20-21 0.01656142601262044 0.0 0.0 0.005063365914686504 0.0 22-23 0.01751080712162416 0.0 0.0 0.018776648600295785 0.0 24-25 0.01835470144073858 0.0 0.0 0.045992240391735745 0.0 26-27 0.021097357977860432 0.0 0.0 0.11519157455911797 0.0 28-29 0.02341806735542508 0.0 0.0 0.35612340266628406 0.0 30-31 0.02394550130487159 0.0 0.0 0.8609831790764843 0.0 32-33 0.025527803153211126 0.0 0.0 1.6015004440993854 0.0 34-35 0.026160723892546936 0.0 0.0 2.698141111788571 0.0 36-37 0.02689913142177205 0.0 0.0 4.240568953549947 0.0 38-39 0.03090762943756553 0.0 0.0 6.176040574438863 0.0 40-41 0.03206798412634786 0.0 0.0 8.374912709681366 0.0 42-43 0.03248993128590506 0.0 0.0 10.180213631846883 0.0 44-45 0.03248993128590506 0.0 0.0 11.80133461886568 0.0 46-47 0.03248993128590506 0.0 0.0 13.746089077264854 0.0 48-49 0.03248993128590506 0.0 0.0 15.828081849310012 0.0 50-51 0.03312285202524088 0.0 0.0 17.791612956309482 0.0 52-53 0.03312285202524088 0.0 0.0 19.593960248358098 0.0 54-55 0.03322833881513018 0.0 0.0 21.30495598036258 0.0 56-57 0.033439312394908785 0.0 0.0 23.08377971826588 0.0 58-59 0.03354479918479809 0.0 0.0 24.833700075739515 0.0 60-61 0.03354479918479809 0.0 0.0 26.61220735327315 0.0 62-63 0.03375577276457669 0.0 0.0 28.29577651990641 0.0 64-65 0.034177719924133904 0.0 0.0 29.97966214690934 0.0 66-67 0.03438869350391251 0.0 0.0 31.74403419459781 0.0 68-69 0.03459966708369111 0.0 0.0 33.655982261341414 0.0 70-71 0.03459966708369111 0.0 0.0 35.70981006048612 0.0 72-73 0.03459966708369111 0.0 0.0 37.698341536689355 0.0 74-75 0.03459966708369111 0.0 0.0 39.46039287500027 0.0 76-77 0.03470515387358041 0.0 0.0 41.25746582755441 0.0 78-79 0.034810640663469714 0.0 0.0 43.089138447192255 0.0 80-81 0.034810640663469714 0.0 0.0 44.9139544254873 0.0 82-83 0.034810640663469714 0.0 0.0 46.832020726044476 0.0 84-85 0.034810640663469714 0.0 0.0 48.81443396843413 0.0 86-87 0.034810640663469714 0.0 0.0 50.48439533917168 0.0 88-89 0.034810640663469714 0.0 0.0 51.965957303166924 0.0 90-91 0.034810640663469714 1.0548678988930216E-4 0.0 53.71978067186646 0.0 92-93 0.034810640663469714 2.1097357977860432E-4 0.0 55.27307365298644 0.0 94-95 0.034810640663469714 2.1097357977860432E-4 0.0 56.67836866789172 0.0 96-97 0.034810640663469714 2.1097357977860432E-4 0.0 58.06309375876859 0.0 98-99 0.034810640663469714 2.1097357977860432E-4 0.0 59.30963115489047 0.0 100-101 0.034810640663469714 2.1097357977860432E-4 0.0 60.543193675855974 0.0 102-103 0.034810640663469714 2.1097357977860432E-4 0.0 61.74764184281202 0.0 104-105 0.034810640663469714 2.1097357977860432E-4 0.0 62.885633332137814 0.0 106-107 0.034810640663469714 2.1097357977860432E-4 0.0 63.942610966828624 0.0 108-109 0.034810640663469714 2.1097357977860432E-4 0.0 64.97849124354157 0.0 110-111 0.034810640663469714 2.1097357977860432E-4 0.0 65.98536265303497 0.0 112-113 0.034810640663469714 2.1097357977860432E-4 0.0 66.95932218408288 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGGTG 40 1.7733853E-4 59.483227 5 TATATGG 130 0.0 54.91918 2 GTATATG 145 0.0 49.315945 1 GATTGTG 85 6.204937E-8 48.986187 9 GGAAACA 445 0.0 48.20772 1 GTGCTAG 100 4.083631E-9 47.672077 1 ACTAGGG 125 1.2732926E-11 47.596626 3 GTAGGGT 50 5.331277E-4 47.596622 4 GAAAACA 495 0.0 45.745934 1 GTACTAG 135 2.7284841E-11 44.140812 1 TGCTAGG 110 9.616997E-9 43.269657 2 GAGAAAA 405 0.0 42.66945 1 CTAGGGC 70 5.4493845E-5 42.496986 4 GTAACAA 75 8.1087244E-5 39.72673 1 GTTAGAG 75 8.184782E-5 39.663857 3 AGGGTAA 90 5.159047E-6 39.655483 8 ATATGGG 185 0.0 38.591854 3 GGGATTG 140 1.893568E-9 38.239216 7 TCTATGG 80 1.1969072E-4 37.18486 2 ATTTAGC 40 1.19930046E-4 37.177017 18-19 >>END_MODULE