FastQCFastQC Report
Thu 26 May 2016
SRR935976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473993
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT75391.590529817950898No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT41390.8732196467036433RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT22910.4833404712727825RNA PCR Primer, Index 26 (100% over 24bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA11520.24304156390495216No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT9730.205277293124582No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT9680.20422242522568898No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC7700.16244965642952533No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC7320.15443266039793835RNA PCR Primer, Index 26 (95% over 22bp)
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC6850.14451690214834395No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6660.1405084041325505No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG5550.11709033677712541RNA PCR Primer, Index 26 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT4770.10063439755439425RNA PCR Primer, Index 26 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA657.232302E-964.327991
TTGCAAG150.004238107759.49367532-33
CTTTACA859.476935E-1056.219421
GTTGTGT602.1927968E-549.578068
TCATAGG1950.048.892692
AGATCTA505.323892E-447.6100045
TGTAAGG909.595715E-846.346192
ATGTGTA909.716132E-846.2728548
GATGTGT951.486278E-743.8374447
GTTTAAG558.3607703E-443.4422761
TCGCTAT355.4512755E-542.49996108-109
AAGGGTG705.4505264E-542.495487
TAGGGTA853.471523E-642.0088275
TATGGGT758.110438E-539.725314
ATGGTTT758.110438E-539.725313
CAGATGT758.171237E-539.6750035
GGGTAAA600.001308418739.662457
GAGATAT300.001309080539.6624522-23
GTCATAG1850.038.745811
GTGTAGG1551.1641532E-1038.5375061
Produced by FastQC (version 0.11.5)