##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935975_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1291672 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26327659034182 33.0 31.0 34.0 29.0 34.0 2 31.30140856192594 33.0 31.0 34.0 28.0 34.0 3 31.517226509516348 34.0 31.0 34.0 28.0 34.0 4 34.70865900940796 37.0 35.0 37.0 32.0 37.0 5 34.84163394422114 37.0 35.0 37.0 32.0 37.0 6 34.94455403538979 37.0 35.0 37.0 32.0 37.0 7 34.89659913662292 37.0 35.0 37.0 32.0 37.0 8 34.875226837773056 37.0 35.0 37.0 32.0 37.0 9 36.50146554233582 39.0 38.0 39.0 32.0 39.0 10-11 36.21446737252182 39.0 37.0 39.0 32.0 39.0 12-13 36.032673542509244 39.0 37.0 39.0 31.0 39.0 14-15 37.541238023275255 40.0 38.0 41.0 32.0 41.0 16-17 37.71513472460501 40.5 38.0 41.0 32.0 41.0 18-19 37.76976740225072 40.5 38.0 41.0 32.0 41.0 20-21 37.87196981896333 41.0 38.0 41.0 32.0 41.0 22-23 37.761378662694554 40.0 38.0 41.0 32.0 41.0 24-25 37.715289949770536 40.0 38.0 41.0 32.0 41.0 26-27 37.699919948717636 40.0 38.0 41.0 32.0 41.0 28-29 37.601680225320365 40.0 38.0 41.0 31.5 41.0 30-31 37.53271805845446 40.0 37.0 41.0 31.0 41.0 32-33 37.437608773744415 40.0 37.0 41.0 31.0 41.0 34-35 37.16537905908001 40.0 37.0 41.0 30.0 41.0 36-37 37.16221920115943 40.0 36.5 41.0 30.5 41.0 38-39 37.31247174205216 40.0 37.0 41.0 31.0 41.0 40-41 37.31379793012468 40.0 36.5 41.0 31.0 41.0 42-43 37.155220520379785 40.0 36.0 41.0 30.5 41.0 44-45 36.9827727162933 40.0 35.5 41.0 30.0 41.0 46-47 36.829192318173654 40.0 35.0 41.0 30.0 41.0 48-49 36.69463029313943 40.0 35.0 41.0 30.0 41.0 50-51 36.47618590478078 39.5 35.0 41.0 30.0 41.0 52-53 36.21533756247716 39.0 35.0 41.0 30.0 41.0 54-55 35.94017289218935 39.0 35.0 41.0 30.0 41.0 56-57 35.68426891656705 38.0 35.0 40.0 30.0 41.0 58-59 35.32075906267226 37.0 35.0 40.0 29.5 41.0 60-61 34.98965101047325 37.0 35.0 39.0 29.0 41.0 62-63 34.55223307465053 36.0 34.0 39.0 29.0 41.0 64-65 34.04470678314618 35.5 34.0 38.5 28.0 40.0 66-67 33.64507243324931 35.0 34.0 37.0 28.0 39.5 68-69 33.40297149740801 35.0 34.0 37.0 28.5 39.0 70-71 33.23464896661072 35.0 34.0 36.0 29.0 39.0 72-73 32.975145780043235 35.0 34.0 36.0 28.5 37.5 74-75 32.65526735889607 35.0 34.0 35.0 27.5 37.0 76-77 32.49666904601168 35.0 34.0 35.0 27.5 36.5 78-79 32.342595101542805 35.0 33.5 35.0 27.0 36.0 80-81 32.153312915353126 35.0 33.0 35.0 27.0 36.0 82-83 32.019899014610516 35.0 33.0 35.0 27.0 35.0 84-85 31.82919541493506 35.0 33.0 35.0 26.0 35.0 86-87 31.657478833635786 35.0 33.0 35.0 25.0 35.0 88-89 31.52988413467196 35.0 33.0 35.0 25.0 35.0 90-91 31.479818405911097 35.0 33.0 35.0 25.0 35.0 92-93 31.38972510048991 35.0 33.0 35.0 24.0 36.0 94-95 31.191379854947698 35.0 33.0 35.0 23.5 35.0 96-97 31.05071372608526 35.0 33.0 35.0 23.0 35.0 98-99 30.86956557082603 35.0 32.0 35.0 20.0 35.0 100-101 30.751345929926483 35.0 32.0 35.0 19.0 35.0 102-103 30.030870066084887 34.0 30.5 35.0 18.0 35.0 104-105 30.521597975337393 35.0 32.0 35.0 18.0 35.0 106-107 30.50367817836107 35.0 32.0 35.0 17.5 35.0 108-109 30.349907716510074 35.0 32.0 35.0 14.0 35.0 110-111 30.227454802767266 35.0 32.0 35.0 9.0 35.0 112-113 30.079243801832042 35.0 31.5 35.0 4.5 35.0 114-115 29.837055769576178 35.0 31.0 35.0 2.0 35.0 116-117 29.55931807765439 35.0 31.0 35.0 2.0 35.0 118-119 29.345424380183204 35.0 31.0 35.0 2.0 35.0 120-121 29.117852287577648 35.0 31.0 35.0 2.0 35.0 122-123 28.61664571191448 35.0 30.5 35.0 2.0 35.0 124-125 25.69777040920605 32.0 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26493553868899866 1101 2 0.44095284893892384 1101 3 0.3630274840258352 1101 4 0.43794724068852986 1101 5 0.35417647441499156 1101 6 0.3146804350787349 1101 7 0.4180518928617545 1101 8 0.35826699045468047 1101 9 0.414749362248898 1101 10-11 0.493828592710841 1101 12-13 0.36935409493489146 1101 14-15 0.4060372980702667 1101 16-17 0.477613162853757 1101 18-19 0.5286016443391972 1101 20-21 0.5358956624257587 1101 22-23 0.44948386793615214 1101 24-25 0.5249527290880494 1101 26-27 0.4915013600540732 1101 28-29 0.3935062820948261 1101 30-31 0.3060108991112003 1101 32-33 0.3607587209194705 1101 34-35 0.041204887986772576 1101 36-37 0.16624376734443302 1101 38-39 0.33754509001329325 1101 40-41 0.21715757747500675 1101 42-43 0.20251738256411755 1101 44-45 0.2411711057149546 1101 46-47 0.23697311539291377 1101 48-49 0.1898885245740729 1101 50-51 0.2853374820222214 1101 52-53 0.24734344485082715 1101 54-55 0.20078080304802626 1101 56-57 0.044241140996525985 1101 58-59 0.08333740176301774 1101 60-61 0.16188111579436537 1101 62-63 0.1438956244950873 1101 64-65 0.19939792513410026 1101 66-67 0.0223419336051478 1101 68-69 0.16453888502231706 1101 70-71 0.019956622527146806 1101 72-73 0.17002089403484177 1101 74-75 0.2602643461428755 1101 76-77 0.22238456937862594 1101 78-79 0.20008851719837395 1101 80-81 0.26899411886810753 1101 82-83 0.18266803269187193 1101 84-85 0.08691190252669401 1101 86-87 0.05774512123377917 1101 88-89 0.021730776817975084 1101 90-91 0.017879157921527167 1101 92-93 -0.1499445373305548 1101 94-95 -0.022056084846770574 1101 96-97 0.07825838014325015 1101 98-99 -0.07926926522763722 1101 100-101 -0.05380669350851619 1101 102-103 0.04741871921931562 1101 104-105 -0.03474243464975402 1101 106-107 0.2349138053925941 1101 108-109 0.26504051663728845 1101 110-111 0.2505481333685111 1101 112-113 0.32668527635959066 1101 114-115 0.3166921861244809 1101 116-117 0.2489682189654161 1101 118-119 0.14076448365718264 1101 120-121 -0.13898601471233363 1101 122-123 -0.0840378859796651 1101 124-125 1.2125725899636564E-4 1102 1 0.22032740173207088 1102 2 0.29336329827744123 1102 3 0.36572474860060566 1102 4 0.4693902724177619 1102 5 0.42584173736054964 1102 6 0.3750754556912739 1102 7 0.4890959208796417 1102 8 0.3473385236971751 1102 9 0.4392300014977124 1102 10-11 0.4591802906994431 1102 12-13 0.4613664078497095 1102 14-15 0.5181433808403355 1102 16-17 0.5562424243679587 1102 18-19 0.5556793282571775 1102 20-21 0.4597203431193577 1102 22-23 0.48871337601703146 1102 24-25 0.49269942584638926 1102 26-27 0.4834808766472136 1102 28-29 0.45731632811246925 1102 30-31 0.5622161815996307 1102 32-33 0.6409106183199853 1102 34-35 0.66233060165208 1102 36-37 0.3870959963057956 1102 38-39 0.3489870231781822 1102 40-41 0.4275146622835351 1102 42-43 0.32879510605611273 1102 44-45 0.3773742938643352 1102 46-47 0.34236799788186545 1102 48-49 0.39773641773965096 1102 50-51 0.3803777530785126 1102 52-53 0.4196110230046912 1102 54-55 0.4250737548261867 1102 56-57 0.33777914136528864 1102 58-59 0.2909210934146387 1102 60-61 0.2823857551408224 1102 62-63 0.32832129055593384 1102 64-65 0.3137488374243347 1102 66-67 0.303811144572542 1102 68-69 0.4325209700717565 1102 70-71 0.39088976621358995 1102 72-73 0.28615986435240615 1102 74-75 0.416416916815578 1102 76-77 0.4571293009274058 1102 78-79 0.3601368154836493 1102 80-81 0.41611131898091713 1102 82-83 0.2843266721295592 1102 84-85 0.22313693347601316 1102 86-87 0.3571814806300715 1102 88-89 0.35395623048971814 1102 90-91 0.3838327856572583 1102 92-93 0.5359730240878626 1102 94-95 0.49490147652025485 1102 96-97 0.4199369479237056 1102 98-99 0.42147804762614527 1102 100-101 0.5805519023078141 1102 102-103 0.5763829143731698 1102 104-105 0.6242810187354628 1102 106-107 0.4693325010305216 1102 108-109 0.5301642464624123 1102 110-111 0.45572686850336197 1102 112-113 0.5231519464954069 1102 114-115 0.5756758406433384 1102 116-117 0.5411842481114206 1102 118-119 0.4234236453947666 1102 120-121 0.4062774062427472 1102 122-123 0.4384349823718523 1102 124-125 0.029995462004407614 1103 1 0.34161517891585547 1103 2 0.29016542664874123 1103 3 0.3508939897587027 1103 4 0.30100352659368923 1103 5 0.3753457530669735 1103 6 0.309745273647998 1103 7 0.35418913091249493 1103 8 0.23870029992431796 1103 9 0.32093282359051045 1103 10-11 0.49320500081515206 1103 12-13 0.39707180599707215 1103 14-15 0.44081813206662446 1103 16-17 0.4251759565326836 1103 18-19 0.5374853175840641 1103 20-21 0.40802089740876823 1103 22-23 0.3356046033632225 1103 24-25 0.46795601526267916 1103 26-27 0.5012500258926877 1103 28-29 0.4671004897536335 1103 30-31 0.4532257824049637 1103 32-33 0.35744113550045853 1103 34-35 0.5093482870170263 1103 36-37 0.5274903452423914 1103 38-39 0.5069712292125246 1103 40-41 0.5144055457389456 1103 42-43 0.472446413310017 1103 44-45 0.5310123456917992 1103 46-47 0.6021232414346898 1103 48-49 0.41716233897616206 1103 50-51 0.51732100172093 1103 52-53 0.5231982727850664 1103 54-55 0.5272610565028089 1103 56-57 0.4995466149247676 1103 58-59 0.4110871613987257 1103 60-61 0.4743679415984019 1103 62-63 0.43204578402044547 1103 64-65 0.4003230287339008 1103 66-67 0.5769420200246884 1103 68-69 0.6942104429497178 1103 70-71 0.6440734132690977 1103 72-73 0.6918682949911954 1103 74-75 0.5575371879265703 1103 76-77 0.5658883554153391 1103 78-79 0.631204971510023 1103 80-81 0.5298746095780089 1103 82-83 0.5253178278839528 1103 84-85 0.6537108559255103 1103 86-87 0.5672013637697297 1103 88-89 0.5437958210721128 1103 90-91 0.4515792693740295 1103 92-93 0.600236832084267 1103 94-95 0.5825118732764274 1103 96-97 0.45846240678342554 1103 98-99 0.6092372553017853 1103 100-101 0.6599059503712432 1103 102-103 0.6701800625807159 1103 104-105 0.519483273408472 1103 106-107 0.5353553103680966 1103 108-109 0.5931125159102173 1103 110-111 0.5418042939494043 1103 112-113 0.49932312907327514 1103 114-115 0.2830419811571119 1103 116-117 0.34739225826557885 1103 118-119 0.2616006803684563 1103 120-121 0.16827466136906466 1103 122-123 0.05597388110852464 1103 124-125 -0.0357192524568255 1104 1 0.21114969140493756 1104 2 0.3044235449698718 1104 3 0.46466986478710126 1104 4 0.2554860412721567 1104 5 0.325895919023921 1104 6 0.3379803668720456 1104 7 0.32387778439713344 1104 8 0.23381688143989976 1104 9 0.3424991461461673 1104 10-11 0.39273449724215936 1104 12-13 0.3230608011532965 1104 14-15 0.4980201451998667 1104 16-17 0.44839879928295545 1104 18-19 0.3031374895084511 1104 20-21 0.4261208008991275 1104 22-23 0.3184737603751131 1104 24-25 0.3675826311893573 1104 26-27 0.523155123218018 1104 28-29 0.5477679156904856 1104 30-31 0.4274766129520202 1104 32-33 0.36362766287611237 1104 34-35 0.44178635237219765 1104 36-37 0.4725178116126685 1104 38-39 0.3367422436920222 1104 40-41 0.4613284432237563 1104 42-43 0.43798774398437956 1104 44-45 0.19643932844660839 1104 46-47 0.30160044274649067 1104 48-49 0.3615328384326233 1104 50-51 0.28653290061297554 1104 52-53 0.25451313664243 1104 54-55 0.4001349856345513 1104 56-57 0.31442297433095234 1104 58-59 0.18323780182329585 1104 60-61 0.23642394768477004 1104 62-63 0.1912679437324698 1104 64-65 0.221164537943487 1104 66-67 0.1891063517068332 1104 68-69 0.2918518265290473 1104 70-71 0.29836351851491827 1104 72-73 0.2407343447889545 1104 74-75 0.305242225181118 1104 76-77 0.3891326955744745 1104 78-79 0.36853773767767706 1104 80-81 0.3578287118477377 1104 82-83 0.42400755318975314 1104 84-85 0.3961657990336107 1104 86-87 0.44706637223909595 1104 88-89 0.453501215092178 1104 90-91 0.44872362066303495 1104 92-93 0.3739339137728912 1104 94-95 0.33891771996178477 1104 96-97 0.22427756260914933 1104 98-99 0.22703360318169885 1104 100-101 0.29003037615410676 1104 102-103 0.27662562611013186 1104 104-105 0.19964467168532352 1104 106-107 0.26820380852252157 1104 108-109 0.29832754336134215 1104 110-111 0.3882098814461443 1104 112-113 0.39965092644266065 1104 114-115 0.32225493205001854 1104 116-117 0.18982860077876396 1104 118-119 0.05738642590926801 1104 120-121 0.24017190680391565 1104 122-123 0.2616299866013385 1104 124-125 0.29684291850703914 1105 1 0.06564733922778743 1105 2 0.012005598377403004 1105 3 0.05163530107064318 1105 4 0.1282511209892263 1105 5 0.032743192064785376 1105 6 0.10842178067746744 1105 7 0.03715240569925271 1105 8 0.09845273836980084 1105 9 0.012778996268572484 1105 10-11 0.05849786804488133 1105 12-13 0.13906695746879905 1105 14-15 0.12791743434429748 1105 16-17 0.1436897811079234 1105 18-19 0.12836491943151884 1105 20-21 0.11551462029626691 1105 22-23 0.12053624926541318 1105 24-25 0.2028646724532166 1105 26-27 0.3155664699868268 1105 28-29 0.21586014426614497 1105 30-31 0.1838225633882189 1105 32-33 0.21884249153588797 1105 34-35 0.23293933276455903 1105 36-37 0.1759969746494292 1105 38-39 0.2018132489947675 1105 40-41 0.15644562969252718 1105 42-43 0.16617840672986972 1105 44-45 0.2341656803984833 1105 46-47 0.21498303716357015 1105 48-49 0.08314083706690667 1105 50-51 0.05507488517114467 1105 52-53 0.08342854802264554 1105 54-55 0.14062331978625764 1105 56-57 0.1751028969634234 1105 58-59 0.15284869382026756 1105 60-61 0.09125598657269052 1105 62-63 0.13333733163842965 1105 64-65 0.14094632137423702 1105 66-67 0.2466000554750707 1105 68-69 0.23273280618927572 1105 70-71 0.15795755016239355 1105 72-73 0.162230322416427 1105 74-75 0.04413208204414332 1105 76-77 0.11358037833239365 1105 78-79 0.14848113759003212 1105 80-81 0.07802610817330446 1105 82-83 0.1460278208082073 1105 84-85 0.13646185832291025 1105 86-87 0.06359989846327707 1105 88-89 0.1517348382897623 1105 90-91 0.21146964008939406 1105 92-93 0.17906796059115848 1105 94-95 0.23487985985340742 1105 96-97 0.12411159904369384 1105 98-99 0.10922367106308428 1105 100-101 0.13389526808419205 1105 102-103 0.16959892586356418 1105 104-105 0.07704282760660064 1105 106-107 0.07012066130421246 1105 108-109 0.12309606554643082 1105 110-111 0.04236856232619246 1105 112-113 -0.08748821719941802 1105 114-115 0.1564189631871038 1105 116-117 -0.12414084995751296 1105 118-119 -0.10480072045017863 1105 120-121 -0.1546119445715739 1105 122-123 -0.036686002395434514 1105 124-125 0.02847164938734892 1106 1 -0.07292255818455473 1106 2 0.05693922852003652 1106 3 0.16723276439433477 1106 4 -0.6736597992037332 1106 5 -0.06140205940705812 1106 6 0.008069445828120081 1106 7 0.09444383575136328 1106 8 0.09834589375216041 1106 9 0.02191715561343699 1106 10-11 0.14124734181410759 1106 12-13 0.152054467585387 1106 14-15 0.09327327739880786 1106 16-17 0.09896689566385675 1106 18-19 0.06496105182461775 1106 20-21 0.12446432929191076 1106 22-23 0.03787805613409034 1106 24-25 -0.043365479266135765 1106 26-27 0.09319127207189837 1106 28-29 0.07228116926778227 1106 30-31 0.04036810022773807 1106 32-33 0.17116960095992084 1106 34-35 0.1829070269904065 1106 36-37 0.11951613348784207 1106 38-39 0.19435726140556397 1106 40-41 0.1697768093075851 1106 42-43 0.04572456108080303 1106 44-45 0.11400670906817822 1106 46-47 0.11856754789093316 1106 48-49 0.07268184268507838 1106 50-51 0.16610789595151942 1106 52-53 0.13784737219341991 1106 54-55 0.11118173855321345 1106 56-57 0.012700340096003515 1106 58-59 0.12431697442956846 1106 60-61 0.1394656241254566 1106 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-0.68906425427312 2216 12-13 -0.8258784233624041 2216 14-15 -0.9964157364795128 2216 16-17 -0.9881401982355271 2216 18-19 -0.9022228014240881 2216 20-21 -0.9843655641567324 2216 22-23 -1.1037872461359512 2216 24-25 -1.0321003786057688 2216 26-27 -0.9973115642679247 2216 28-29 -1.0041451067640992 2216 30-31 -0.8400585867283041 2216 32-33 -0.8358931580747964 2216 34-35 -0.8140270935195275 2216 36-37 -0.9122727028584023 2216 38-39 -0.931474619246643 2216 40-41 -0.9483847545156792 2216 42-43 -0.9278270115067073 2216 44-45 -0.9923704807884235 2216 46-47 -1.0370619526114808 2216 48-49 -1.0412252114060223 2216 50-51 -1.0753606121690567 2216 52-53 -0.9174901474298167 2216 54-55 -0.9393635385477097 2216 56-57 -0.8925583655570932 2216 58-59 -1.0256013222897806 2216 60-61 -0.9190565577534286 2216 62-63 -0.9534075274714553 2216 64-65 -0.7531345745510976 2216 66-67 -0.8412889458240755 2216 68-69 -1.0954638641502967 2216 70-71 -0.8800492470567178 2216 72-73 -1.0290725060946926 2216 74-75 -1.0010831397437805 2216 76-77 -1.0862283676858908 2216 78-79 -1.3807898334184614 2216 80-81 -1.2733293433636312 2216 82-83 -1.378264769602982 2216 84-85 -1.348541236872915 2216 86-87 -1.3320302392564969 2216 88-89 -1.2371739337488314 2216 90-91 -1.219704289249961 2216 92-93 -1.0556885522362869 2216 94-95 -1.0948702794069227 2216 96-97 -1.2685414675485767 2216 98-99 -1.202000382600751 2216 100-101 -1.196777089233855 2216 102-103 -1.1720179311646923 2216 104-105 -1.5708236947741554 2216 106-107 -1.326057124113703 2216 108-109 -1.489266353234033 2216 110-111 -1.7228617737867147 2216 112-113 -1.9381459580540294 2216 114-115 -1.9110560709351994 2216 116-117 -1.8119746168061326 2216 118-119 -1.862412365901367 2216 120-121 -1.6566047997887416 2216 122-123 -1.3650888625630344 2216 124-125 -1.1820977163464406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15407.0 3 3653.0 4 2518.0 5 1800.0 6 2141.0 7 2316.0 8 2532.0 9 2953.0 10 3373.0 11 4103.0 12 4166.0 13 3891.0 14 3910.0 15 3898.0 16 3757.0 17 3800.0 18 3594.0 19 3684.0 20 3751.0 21 4100.0 22 4900.0 23 6689.0 24 9293.0 25 13898.0 26 16901.0 27 15179.0 28 17030.0 29 21664.0 30 24734.0 31 30992.0 32 42491.0 33 58287.0 34 96081.0 35 130248.0 36 261396.0 37 415756.0 38 46706.0 39 80.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 72.5837943125478 9.263239679726064 6.065773088118755 12.087192919607396 2 10.756195937747542 33.2158943699761 43.456081570264764 12.571828122011597 3 39.88011406352468 19.470547655124303 17.505561100095157 23.14377718125586 4 15.052560342184243 14.729284813870727 48.72602340901982 21.492131434925216 5 16.919488116888598 32.67160773693253 27.60921724063446 22.79968690554442 6 31.475173263800716 39.187967223877266 13.723375593803999 15.613483918518014 7 46.69470786291556 21.686714341345436 15.97117222574479 15.647405569994216 8 43.87125793934664 22.739434591551387 15.2128101029057 18.17649736619627 9 58.68502220377929 9.81967558327501 9.573405632389646 21.921896580556055 10-11 40.68908360636446 25.08156095355477 19.066334177716943 15.163021262363822 12-13 28.92108058392533 29.09569921775807 24.976967837036028 17.006252361280573 14-15 24.963690472503856 29.596522956292308 22.643093602710284 22.79669296849355 16-17 22.497623235620186 36.01777386209502 20.644985723929913 20.839617178354878 18-19 23.68240544039044 26.66040604735568 24.228170928842616 25.429017583411266 20-21 24.11680364674623 27.963716794975813 21.387279433168793 26.532200125109163 22-23 24.61112418632594 24.75024619253185 22.054321840219497 28.584307780922714 24-25 21.3668795174007 22.819454164834415 34.982255557138345 20.831410760626536 26-27 16.90166251952922 23.15318188926756 31.87827390307311 28.066881688130103 28-29 21.147274625616454 24.08903520674473 21.69339862406329 33.07029154357552 30-31 21.39118909444503 29.402046339937694 20.490302491654226 28.71646207396305 32-33 31.00876228640088 25.38678549972439 18.311498584780036 25.292953629094693 34-35 30.4856805752544 17.316470435218847 25.731377625279485 26.46647136424727 36-37 25.436294972717533 24.676040047318516 29.608406778191366 20.27925820177259 38-39 23.075517623669167 30.08766931542992 24.113010114022753 22.723802946878155 40-41 20.2898259000737 29.599542298664055 26.493606736075414 23.617025065186827 42-43 21.730555435125947 21.64322676345078 24.255190172118 32.371027629305274 44-45 22.572293895044563 22.97669222527081 23.503761016728706 30.94725286295592 46-47 24.00853312605677 22.0835862355149 23.197568732619427 30.710311905808908 48-49 16.732614781461546 22.00121238209081 25.042619178862747 36.2235536575849 50-51 21.956154503620116 24.991948420342005 23.78572114282883 29.26617593320905 52-53 24.27644169727299 22.28553378876371 28.602539963705958 24.83548455025734 54-55 19.574822400733314 24.58371784787469 29.629697012863947 26.211762738528048 56-57 20.385834888494824 26.820351847787332 25.871074305945076 26.92273895777277 58-59 24.873613425080052 22.34359806514347 30.60715104144086 22.175637468335616 60-61 25.64606184851882 29.089544404461815 27.548712056930864 17.715681690088505 62-63 26.785824884335963 25.310179364420687 25.298992313838188 22.60500343740516 64-65 32.40643785446659 18.191386696695638 28.10776055120558 21.2944148976322 66-67 32.501658704112735 23.639456177308308 22.93811659470562 20.92076852387334 68-69 23.434225186031693 28.491399300547158 25.04558366838781 23.028791845033336 70-71 25.372716523268984 27.956701153866703 22.188219424390795 24.482362898473514 72-73 24.185143407482876 27.70833704300405 22.698539641347747 25.40797990816533 74-75 20.39438128996192 28.017942893068902 27.56147302991308 24.026202787056093 76-77 22.18323721059145 28.3982411841465 23.621846075354707 25.796675529907343 78-79 22.927433711904936 30.694299980606253 18.667428997018163 27.710837310470648 80-81 21.704172036051297 22.052986874636854 24.311051016154025 31.931790073157824 82-83 24.546982515685094 17.028897429068678 28.76558445178033 29.658535603465896 84-85 26.25682209584696 18.207639508375458 33.1959234686292 22.33961492714838 86-87 29.26917358196805 22.102705822556665 25.138457643593554 23.489662951881733 88-89 24.039871380146778 26.500684333505625 26.641577664593342 22.817866621754256 90-91 19.193826908642723 29.024662249389454 26.107625745110692 25.67388509685713 92-93 23.98888397630915 21.924916509803953 27.39961237939122 26.686587134495674 94-95 21.775561703574137 20.83802758555541 30.094783243629564 27.291627467240893 96-97 21.422712060592335 20.47956673305809 29.458242117712185 28.639479088637387 98-99 20.34233514422485 22.17804028865874 31.94322827994726 25.53639628716915 100-101 18.633192945511976 27.266734279918865 28.722999860644443 25.37707291392472 102-103 20.117336289708224 26.291194668615564 26.643877083346233 26.94759195832998 104-105 20.538142629861458 21.97446706382847 25.928820553316847 31.558569752993233 106-107 19.85408625455666 23.774603637895147 23.21597858868497 33.15533151886322 108-109 22.24918555174998 24.601872611622767 25.4980753627856 27.650866473841656 110-111 23.403774332802755 26.443865005976754 23.326239169076977 26.826121492143518 112-113 20.012983353127076 26.70630060372205 25.83915661624553 27.44155942690535 114-115 20.892001331691944 22.5723720375661 27.928057665358203 28.607568965383752 116-117 23.883191708111656 21.12002892375154 27.091281687611097 27.905497680525706 118-119 20.81464634079728 21.44222712148453 28.960606134563392 28.782520403154795 120-121 18.796208086828912 23.022916152947776 30.482017677478346 27.69885808274497 122-123 20.262272108764535 25.639643929320087 30.890079920408642 23.20800404150674 124-125 20.135492163233245 28.42933249716318 25.814101678497746 25.621073661105825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 8.5 2 15.0 3 16.0 4 21.5 5 32.5 6 37.0 7 44.5 8 58.0 9 68.0 10 65.5 11 83.0 12 109.0 13 129.5 14 173.5 15 226.5 16 269.5 17 328.0 18 412.0 19 477.0 20 564.5 21 680.5 22 802.5 23 980.0 24 1188.0 25 1452.0 26 1804.0 27 2154.0 28 2439.0 29 2857.5 30 3469.0 31 3961.5 32 4593.0 33 5372.5 34 6380.0 35 7206.5 36 7935.0 37 8939.5 38 9669.5 39 10002.0 40 10194.5 41 11070.5 42 12102.5 43 13446.0 44 15085.5 45 20164.5 46 39588.5 47 82395.5 48 125011.0 49 154115.5 50 153076.0 51 116610.0 52 79824.0 53 55841.5 54 43765.0 55 38949.5 56 35372.0 57 30717.0 58 26863.5 59 22742.5 60 19810.5 61 17371.5 62 13911.5 63 10721.5 64 8541.5 65 7615.5 66 6640.0 67 5888.5 68 5382.0 69 4828.5 70 4116.5 71 3084.0 72 2259.0 73 1701.0 74 1427.0 75 1302.0 76 1056.0 77 844.0 78 712.0 79 608.0 80 574.0 81 504.5 82 332.0 83 151.5 84 66.5 85 59.5 86 46.5 87 23.5 88 19.5 89 20.5 90 22.0 91 21.5 92 14.0 93 10.5 94 9.0 95 4.5 96 2.0 97 0.5 98 0.5 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15685096526053052 2 0.017109606773236547 3 0.008593512904204782 4 0.015793483175295275 5 0.0037935327234777873 6 0.0 7 7.741903517301606E-5 8 3.096761406920642E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.001393542633114289 28-29 0.008941898562483354 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.2580469345158837E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.00236128057277699 66-67 7.741903517301606E-5 68-69 0.007045132200744462 70-71 0.021290234672579414 72-73 2.3225710551904818E-4 74-75 0.010064474572492089 76-77 5.806427637976204E-4 78-79 0.001432252150700797 80-81 9.677379396627007E-4 82-83 0.0 84-85 0.021677329848444497 86-87 0.0024386996079500058 88-89 0.020864429979127828 90-91 1.1612855275952409E-4 92-93 0.01571606414012226 94-95 0.015406387999430195 96-97 0.006929003647984937 98-99 0.0033290185124396905 100-101 9.290284220761927E-4 102-103 0.0 104-105 5.419332462111125E-4 106-107 0.001432252150700797 108-109 0.0 110-111 0.0 112-113 0.001393542633114289 114-115 0.006348360884187317 116-117 0.0 118-119 0.07753516372577558 120-121 0.002206442502430958 122-123 0.005767718120389696 124-125 0.2522312165936863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1291672.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.142154256461836 #Duplication Level Percentage of deduplicated Percentage of total 1 79.63721361584327 27.986232454427668 2 10.441314319275598 7.338605568963737 3 3.19346943099561 3.3667618607202936 4 1.6181615921901789 2.274627371385166 5 0.968521095044294 1.7017958861341955 6 0.645290725855081 1.3606143736958087 7 0.4569108744766505 1.1239782702619325 8 0.3686057122731081 1.0362879040411643 9 0.26986457681539466 0.8535260328121254 >10 1.9748221308661078 13.759472106246578 >50 0.21787258661830036 5.358441835164987 >100 0.1707244050303712 12.20764656568843 >500 0.02489271966221654 6.078973224965048 >1k 0.010133319508512928 7.035893798461297 >5k 8.81158218131559E-4 1.7875006910467106 >10k+ 0.0013217373271973386 6.729642055984867 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 18430 1.426832818238686 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 16697 1.2926656302838493 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 15082 1.1676338884794282 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 14461 1.1195566676369852 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 11348 0.8785512111433863 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10912 0.8447965118079513 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 6424 0.49733988195145523 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 6057 0.4689270960429583 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 5555 0.4300627403861042 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 5054 0.3912758037644232 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 4901 0.3794306913829517 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 4673 0.361779151363504 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 3824 0.2960503905016134 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 3575 0.2767730507435324 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 3565 0.27599886039180227 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 3439 0.2662440619600022 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 3336 0.2582699013371816 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 3067 0.23744418087564026 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2723 0.21081203277612273 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2398 0.1856508463448925 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 2339 0.18108312326968456 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 2159 0.16714769693854167 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2159 0.16714769693854167 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2126 0.16459286877783214 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 2123 0.16436061167231308 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2092 0.1619606215819496 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2052 0.15886386017502896 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2043 0.1581670888584718 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1955 0.1513542137632464 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1933 0.14965099498944004 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1895 0.14670907165286545 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1811 0.1402058726983321 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1795 0.1389671681355638 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1644 0.1272768938244384 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 1602 0.12402529434717173 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1600 0.12387045627682569 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1391 0.10768987792566533 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGT 1370 0.10606407818703201 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 1365 0.10567698301116692 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1334 0.10327699292080343 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.741903517301606E-5 0.0 3 0.0 0.0 0.0 7.741903517301606E-5 0.0 4 0.0 0.0 0.0 1.548380703460321E-4 0.0 5 0.0 0.0 0.0 1.548380703460321E-4 0.0 6 0.0 0.0 0.0 1.548380703460321E-4 0.0 7 0.0 0.0 0.0 1.548380703460321E-4 0.0 8 0.0 0.0 0.0 1.548380703460321E-4 0.0 9 0.0 0.0 0.0 1.548380703460321E-4 0.0 10-11 0.0 0.0 0.0 1.548380703460321E-4 0.0 12-13 0.0 0.0 0.0 1.548380703460321E-4 0.0 14-15 0.0 0.0 0.0 1.548380703460321E-4 0.0 16-17 0.0 0.0 0.0 1.9354758793254013E-4 0.0 18-19 0.0 0.0 0.0 2.709666231055562E-4 0.0 20-21 0.0 0.0 0.0 9.290284220761927E-4 0.0 22-23 0.0 0.0 0.0 0.0018193473265658774 0.0 24-25 0.0 0.0 0.0 0.00294192333657461 0.0 26-27 0.0 0.0 0.0 0.0050322372862460435 0.0 28-29 0.0 0.0 0.0 0.012038659969403998 0.0 30-31 0.0 0.0 0.0 0.0414578933351501 0.0 32-33 0.0 0.0 0.0 0.09774153190593277 0.0 34-35 0.0 0.0 0.0 0.16199933109953613 0.0 36-37 0.0 0.0 0.0 0.24112158504635853 0.0 38-39 0.0 0.0 0.0 0.368940412117008 0.0 40-41 0.0 0.0 0.0 0.5756879455465475 0.0 42-43 3.870951758650803E-5 0.0 0.0 1.0693891328448708 0.0 44-45 7.741903517301606E-5 0.0 0.0 1.7562508128998693 0.0 46-47 7.741903517301606E-5 0.0 0.0 2.4434995881307326 0.0 48-49 7.741903517301606E-5 0.0 0.0 2.685937296775033 0.0 50-51 7.741903517301606E-5 0.0 0.0 3.007652097436501 0.0 52-53 7.741903517301606E-5 0.0 0.0 3.262902656401935 0.0 54-55 7.741903517301606E-5 0.0 0.0 3.51048873088524 0.0 56-57 7.741903517301606E-5 0.0 0.0 3.9360611672313093 0.0 58-59 7.741903517301606E-5 0.0 0.0 4.431620411373785 0.0 60-61 7.741903517301606E-5 0.0 0.0 5.190752760762795 0.0 62-63 7.741903517301606E-5 0.0 0.0 6.053742745836404 0.0 64-65 7.741903517301606E-5 0.0 0.0 6.76890108324714 0.0 66-67 7.741903517301606E-5 0.0 0.0 7.325814912764232 0.0 68-69 7.741903517301606E-5 0.0 0.0 7.795942003852371 0.0 70-71 7.741903517301606E-5 0.0 0.0 8.349371976786676 0.0 72-73 7.741903517301606E-5 0.0 0.0 8.880079462897701 0.0 74-75 7.741903517301606E-5 0.0 0.0 9.425419146656427 0.0 76-77 7.741903517301606E-5 0.0 0.0 10.084990616812938 0.0 78-79 7.741903517301606E-5 0.0 0.0 10.972057921825355 0.0 80-81 7.741903517301606E-5 0.0 0.0 13.738046500969286 0.0 82-83 7.741903517301606E-5 0.0 0.0 15.579690509664992 0.0 84-85 7.741903517301606E-5 0.0 0.0 16.219597544887556 0.0 86-87 7.741903517301606E-5 0.0 0.0 16.726072872989427 0.0 88-89 7.741903517301606E-5 0.0 0.0 17.35812187614193 0.0 90-91 7.741903517301606E-5 0.0 0.0 18.264737487535534 0.0 92-93 7.741903517301606E-5 0.0 0.0 19.475261521500812 0.0 94-95 7.741903517301606E-5 0.0 0.0 20.897720164252227 0.0 96-97 7.741903517301606E-5 0.0 0.0 22.376385026539246 0.0 98-99 7.741903517301606E-5 0.0 0.0 23.811811357682135 0.0 100-101 7.741903517301606E-5 0.0 0.0 25.16083804557194 0.0 102-103 7.741903517301606E-5 0.0 0.0 26.338226732483168 0.0 104-105 1.548380703460321E-4 0.0 0.0 27.500518707535658 0.0 106-107 1.548380703460321E-4 0.0 0.0 28.630062430709962 0.0 108-109 1.548380703460321E-4 0.0 0.0 29.534781275741828 0.0 110-111 1.548380703460321E-4 0.0 0.0 30.57358988969336 0.0 112-113 1.548380703460321E-4 0.0 0.0 34.72774047900705 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 3190 0.0 98.62678 1 AGTAGTA 2785 0.0 95.71169 2 GAGTCCG 725 0.0 91.229774 1 GAGTTAG 1465 0.0 91.10915 1 GAGTAAG 1170 0.0 90.144455 1 AGTCCGT 330 0.0 88.34751 2 GAGTACG 650 0.0 88.00543 1 GAGTCAG 7100 0.0 87.11453 1 AGTACGG 385 0.0 86.544495 2 GAGTCGA 450 0.0 83.421814 1 GAGTCGG 935 0.0 80.93637 1 AGTTAGG 360 0.0 79.33246 2 AGTTAGT 1100 0.0 78.430954 2 GAGTCGT 815 0.0 78.230804 1 AGTCCGG 320 0.0 78.09289 2 GAGTTAC 735 0.0 77.01723 1 AGTCGTA 560 0.0 76.49915 2 AGTCAGA 1390 0.0 76.1934 2 GAGTATA 385 0.0 75.83802 1 AGTAAGG 615 0.0 75.46258 2 >>END_MODULE