##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935975_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1291672 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.557811116134744 33.0 31.0 34.0 31.0 34.0 2 32.62467716262333 34.0 31.0 34.0 31.0 34.0 3 32.82111248056782 34.0 31.0 34.0 31.0 34.0 4 36.046330647408936 37.0 35.0 37.0 35.0 37.0 5 36.084445586805316 37.0 35.0 37.0 35.0 37.0 6 36.210690484890904 37.0 37.0 37.0 35.0 37.0 7 36.24925135792988 37.0 37.0 37.0 35.0 37.0 8 36.241132423711285 37.0 37.0 37.0 35.0 37.0 9 38.06993571123319 39.0 39.0 39.0 35.0 39.0 10-11 37.755091462848156 39.0 38.0 39.0 35.0 39.0 12-13 37.646949457757074 39.0 37.0 39.0 35.0 39.0 14-15 39.24045500715352 41.0 39.0 41.0 35.5 41.0 16-17 39.336898221839604 41.0 39.0 41.0 35.5 41.0 18-19 39.31541134281768 41.0 39.0 41.0 35.0 41.0 20-21 39.31114439269412 41.0 39.0 41.0 35.0 41.0 22-23 39.18788670808069 41.0 39.0 41.0 35.0 41.0 24-25 39.01617941706563 41.0 38.0 41.0 35.0 41.0 26-27 38.91848704624704 41.0 38.0 41.0 35.0 41.0 28-29 38.81401663889904 41.0 38.0 41.0 35.0 41.0 30-31 38.68960850742293 40.0 38.0 41.0 35.0 41.0 32-33 38.55193655974582 40.0 38.0 41.0 35.0 41.0 34-35 38.177400299766504 40.0 37.0 41.0 34.0 41.0 36-37 38.056830216959106 40.0 38.0 41.0 34.0 41.0 38-39 37.782987863792044 40.0 37.5 41.0 33.5 41.0 40-41 37.577620324664466 40.0 37.0 41.0 33.0 41.0 42-43 37.40086840931754 40.0 37.0 41.0 33.0 41.0 44-45 37.23734392322509 40.0 36.5 41.0 33.0 41.0 46-47 37.07820948352213 40.0 36.0 41.0 33.0 41.0 48-49 36.900156154193944 40.0 35.5 41.0 32.0 41.0 50-51 36.64153244786603 40.0 35.0 41.0 31.5 41.0 52-53 36.352097513919944 39.0 35.0 41.0 31.0 41.0 54-55 36.105110275673695 39.0 35.0 41.0 31.0 41.0 56-57 35.88161003722307 39.0 35.0 41.0 31.0 41.0 58-59 35.56286425656049 37.5 35.0 40.0 31.0 41.0 60-61 35.232596200893106 37.0 35.0 39.5 30.0 41.0 62-63 34.81108903808398 36.5 35.0 39.0 29.5 41.0 64-65 34.3334612037731 36.0 35.0 39.0 29.0 41.0 66-67 33.83755976749515 35.0 34.0 37.0 29.0 39.5 68-69 33.472792241373966 35.0 34.0 37.0 29.0 39.0 70-71 33.315050570113776 35.0 34.0 36.0 29.0 39.0 72-73 33.02583318365653 35.0 34.0 36.0 28.5 37.5 74-75 32.71164506159458 35.0 34.0 35.0 27.5 37.0 76-77 32.58601951579038 35.0 34.0 35.0 28.0 36.5 78-79 32.608071166673895 35.0 34.0 35.0 29.0 36.0 80-81 32.517987925727276 35.0 34.0 35.0 29.0 36.0 82-83 32.40273498225555 35.0 34.0 35.0 29.0 35.5 84-85 32.24046623291362 35.0 34.0 35.0 27.5 35.0 86-87 32.15797121869949 35.0 34.0 35.0 27.0 35.0 88-89 32.12421961612546 35.0 34.0 35.0 27.0 35.0 90-91 32.161164366805195 35.0 34.0 35.0 27.0 35.0 92-93 32.17811100650939 35.0 34.0 35.0 27.0 36.0 94-95 32.06763210784162 35.0 34.0 35.0 27.0 35.0 96-97 32.03097535597272 35.0 34.0 35.0 27.0 35.0 98-99 31.93441639982906 35.0 34.0 35.0 27.0 35.0 100-101 31.907517930248545 35.0 34.0 35.0 27.0 35.0 102-103 31.22732977102546 34.5 32.5 35.0 24.5 35.0 104-105 31.77912117008033 35.0 33.5 35.0 26.0 35.0 106-107 31.87746889303167 35.0 34.0 35.0 27.0 35.0 108-109 31.818407459478877 35.0 34.0 35.0 27.0 35.0 110-111 31.747152140791158 35.0 34.0 35.0 26.0 35.0 112-113 31.705730634402542 35.0 34.0 35.0 26.0 35.0 114-115 31.59394838627763 35.0 34.0 35.0 25.0 35.0 116-117 31.445903449172853 35.0 33.5 35.0 25.0 35.0 118-119 31.245439631733134 35.0 33.0 35.0 23.5 35.0 120-121 30.961532803993585 35.0 33.0 35.0 19.0 35.0 122-123 30.613582240692686 35.0 33.0 35.0 2.0 35.0 124-125 28.311889163812484 33.0 29.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07536652795874943 1101 2 0.12917348609025936 1101 3 0.10491301405664188 1101 4 0.14096289944009754 1101 5 0.22476240423049632 1101 6 0.23520866631042026 1101 7 0.19152625923931055 1101 8 0.2065574672580297 1101 9 0.2644117175916776 1101 10-11 0.19448598393993421 1101 12-13 0.23422414551313153 1101 14-15 0.3228151702346551 1101 16-17 0.24701145000568658 1101 18-19 0.2982919459043174 1101 20-21 0.26201761750064634 1101 22-23 0.3186821237399684 1101 24-25 0.36197024563687563 1101 26-27 0.28919726151786307 1101 28-29 0.36175171162116726 1101 30-31 0.3278643358862112 1101 32-33 0.4174975687754028 1101 34-35 0.5618030655420014 1101 36-37 0.5399414786712953 1101 38-39 0.5835011754726764 1101 40-41 0.5128007507770747 1101 42-43 0.3922625922617655 1101 44-45 0.4098448990629251 1101 46-47 0.49855962202753545 1101 48-49 0.47609565757116457 1101 50-51 0.4388293488312769 1101 52-53 0.4530508200872134 1101 54-55 0.5326084001194005 1101 56-57 0.4691586661685392 1101 58-59 0.3493271335887016 1101 60-61 0.3431115797642903 1101 62-63 0.43379247188621406 1101 64-65 0.4216302639950342 1101 66-67 0.2077043075529872 1101 68-69 0.3173977519203248 1101 70-71 0.26803395251727125 1101 72-73 0.34537688553764667 1101 74-75 0.4373928649092349 1101 76-77 0.4065089472070156 1101 78-79 0.38798153312121286 1101 80-81 0.4329624159252745 1101 82-83 0.3327842045046907 1101 84-85 0.36832028975149456 1101 86-87 0.2773990072061494 1101 88-89 0.16236467395196996 1101 90-91 0.3338329112192113 1101 92-93 0.32694614956070467 1101 94-95 0.353429477807353 1101 96-97 0.34997514971975363 1101 98-99 0.34732014775265796 1101 100-101 0.237520352464351 1101 102-103 0.3217937962228987 1101 104-105 0.4181448541965942 1101 106-107 0.37027150811365317 1101 108-109 0.30888265981201357 1101 110-111 0.25976334644088794 1101 112-113 0.34696157200960087 1101 114-115 0.36340098613438343 1101 116-117 0.3341400858739796 1101 118-119 0.06341403974774451 1101 120-121 0.2545039401200846 1101 122-123 0.33093871902464045 1101 124-125 0.48945830263225076 1102 1 0.0038972077773564706 1102 2 0.020505877613459234 1102 3 0.052928756291038326 1102 4 0.14442434079506938 1102 5 0.18602002271002505 1102 6 0.13160216306644656 1102 7 0.16644944003923 1102 8 0.15149144739521603 1102 9 0.10376017256692194 1102 10-11 0.21817963995566458 1102 12-13 0.2147328193532374 1102 14-15 0.23550580487720651 1102 16-17 0.2155377419912412 1102 18-19 0.15122806792076204 1102 20-21 0.2613861036083023 1102 22-23 0.17306950708174185 1102 24-25 0.2197739392543454 1102 26-27 0.2719507648781274 1102 28-29 0.2977892478304298 1102 30-31 0.18776220794779164 1102 32-33 0.22367098647333705 1102 34-35 0.3340729243411502 1102 36-37 0.344848174581621 1102 38-39 0.37738287872282683 1102 40-41 0.2933191575118457 1102 42-43 0.41412675317381087 1102 44-45 0.4116523907578369 1102 46-47 0.429163119316776 1102 48-49 0.5171940099173753 1102 50-51 0.5052179695034269 1102 52-53 0.5805488494169566 1102 54-55 0.59641410969882 1102 56-57 0.5924521171189241 1102 58-59 0.518869983059048 1102 60-61 0.5183333966098402 1102 62-63 0.47327911469283634 1102 64-65 0.5442534943267177 1102 66-67 0.5548011745888033 1102 68-69 0.6175896152203677 1102 70-71 0.6453598091886761 1102 72-73 0.6296625328265364 1102 74-75 0.7377295973676965 1102 76-77 0.6441295403418223 1102 78-79 0.6172239670001787 1102 80-81 0.6167405072114818 1102 82-83 0.6834493177628218 1102 84-85 0.6784344055324709 1102 86-87 0.5955663705938861 1102 88-89 0.6815185609745882 1102 90-91 0.6778677710430401 1102 92-93 0.6406718759462251 1102 94-95 0.6716418116655518 1102 96-97 0.688694018108535 1102 98-99 0.6441960812914438 1102 100-101 0.5974138880877078 1102 102-103 0.5405065550520476 1102 104-105 0.6031827610910234 1102 106-107 0.6022942413289059 1102 108-109 0.6366557156903951 1102 110-111 0.6502059650842291 1102 112-113 0.6375318425455241 1102 114-115 0.6594289050873172 1102 116-117 0.6727836287247335 1102 118-119 0.6688579079277268 1102 120-121 0.8284297722504554 1102 122-123 0.97830791438086 1102 124-125 0.6553391103275992 1103 1 0.005916471913607779 1103 2 0.07037535543094009 1103 3 0.023710857713808764 1103 4 0.13964300843714028 1103 5 0.07872391086423391 1103 6 0.15975962752845874 1103 7 0.12271653688200246 1103 8 0.1518956753760463 1103 9 0.12466654361256957 1103 10-11 0.16840019899701986 1103 12-13 0.1432299541177784 1103 14-15 0.16196210417473367 1103 16-17 0.22867265034435036 1103 18-19 0.21736467384381086 1103 20-21 0.16380803258548582 1103 22-23 0.22238891596476407 1103 24-25 0.2780959398233236 1103 26-27 0.3130938578318023 1103 28-29 0.3793626419386342 1103 30-31 0.323355372649587 1103 32-33 0.34330496163191526 1103 34-35 0.46676724607911524 1103 36-37 0.42433462059545946 1103 38-39 0.604813795754481 1103 40-41 0.5874752026428212 1103 42-43 0.5571917266473321 1103 44-45 0.5370093337563873 1103 46-47 0.6731254914128542 1103 48-49 0.6871324847304976 1103 50-51 0.649201287674309 1103 52-53 0.5651347267604905 1103 54-55 0.3907808660897132 1103 56-57 0.347233136848871 1103 58-59 0.4333305903701685 1103 60-61 0.3797255602768459 1103 62-63 0.38513396777331366 1103 64-65 0.3877456739377436 1103 66-67 0.4120461717595134 1103 68-69 0.4104452842598789 1103 70-71 0.4261070838542409 1103 72-73 0.3599277351437351 1103 74-75 0.48393648206111806 1103 76-77 0.5036775115425272 1103 78-79 0.41946550963168505 1103 80-81 0.4934342474613871 1103 82-83 0.47829142500047794 1103 84-85 0.50519129663477 1103 86-87 0.5475345200550734 1103 88-89 0.5212957822745565 1103 90-91 0.5856970699122641 1103 92-93 0.5923475619301826 1103 94-95 0.5748796996759609 1103 96-97 0.613272517431696 1103 98-99 0.5664459710900793 1103 100-101 0.4694002324974491 1103 102-103 0.5649185799747514 1103 104-105 0.5582785971989317 1103 106-107 0.48509516920876194 1103 108-109 0.41348904433431244 1103 110-111 0.49625200268087255 1103 112-113 0.4597625057232655 1103 114-115 0.41487801081497366 1103 116-117 0.385245761660407 1103 118-119 0.3613407680986107 1103 120-121 0.48663000422041947 1103 122-123 0.30365454065599096 1103 124-125 0.5098925368865963 1104 1 0.033163599112711495 1104 2 0.059165600181223965 1104 3 0.1029445132033473 1104 4 0.14828893086655626 1104 5 0.19288948360247105 1104 6 0.2391942304286303 1104 7 0.12701845807951173 1104 8 0.13164603099807692 1104 9 0.11330554418125871 1104 10-11 0.2154456774515907 1104 12-13 0.17517052979094672 1104 14-15 0.28474615584169527 1104 16-17 0.2614083252181487 1104 18-19 0.25453674306852037 1104 20-21 0.246339634538856 1104 22-23 0.2911813866878816 1104 24-25 0.23429794228409406 1104 26-27 0.23217700096643057 1104 28-29 0.25101609760900345 1104 30-31 0.2035315599240377 1104 32-33 0.2439200009752227 1104 34-35 0.2959854264835471 1104 36-37 0.20011817065621074 1104 38-39 0.24830335883871157 1104 40-41 0.24582450656892263 1104 42-43 0.07037588930800354 1104 44-45 0.10356881830645648 1104 46-47 0.1670924951771866 1104 48-49 0.18227254568556361 1104 50-51 0.25913370043065953 1104 52-53 0.293399855371419 1104 54-55 0.2701741512749507 1104 56-57 0.2895780010264133 1104 58-59 0.23282120850452515 1104 60-61 0.12483215540745363 1104 62-63 0.042037304140677634 1104 64-65 0.18050379149655527 1104 66-67 0.1754051342732197 1104 68-69 0.12055440266792061 1104 70-71 0.25429674283824966 1104 72-73 0.25991691020735175 1104 74-75 0.2355554199061558 1104 76-77 0.2548190849394132 1104 78-79 0.27961968112002467 1104 80-81 0.3231712624928065 1104 82-83 0.3241958636817799 1104 84-85 0.38153271207905703 1104 86-87 0.39872107030030435 1104 88-89 0.3948782111158451 1104 90-91 0.3692244135491123 1104 92-93 0.3450044739569904 1104 94-95 0.37988737623295776 1104 96-97 0.2888720435739174 1104 98-99 0.30228352386164303 1104 100-101 0.4014725369739409 1104 102-103 0.44650551564870966 1104 104-105 0.4246583268194648 1104 106-107 0.4375114932128241 1104 108-109 0.4154950117710712 1104 110-111 0.3817275299391234 1104 112-113 0.35870913268833604 1104 114-115 0.18296060965350236 1104 116-117 0.2559013411527964 1104 118-119 0.21017419062332365 1104 120-121 -2.2486387544162767E-4 1104 122-123 -0.037949349002840194 1104 124-125 -0.044860753665368236 1105 1 0.06463859810073558 1105 2 0.08276656956332573 1105 3 0.13065673273284517 1105 4 0.2190830392479839 1105 5 0.1333731538788001 1105 6 0.1231341315544725 1105 7 0.16508712088484145 1105 8 0.15251995324687329 1105 9 0.13699715114152156 1105 10-11 0.22833117315114038 1105 12-13 0.17758210535364327 1105 14-15 0.22823318586825536 1105 16-17 0.2131460147870854 1105 18-19 0.2650493821708082 1105 20-21 0.25230367517968944 1105 22-23 0.2804518333815409 1105 24-25 0.2284016598327625 1105 26-27 0.11111951562243405 1105 28-29 0.1840798061467055 1105 30-31 0.2400021854289207 1105 32-33 0.22084349757939492 1105 34-35 0.28770264779938515 1105 36-37 0.360449886592761 1105 38-39 0.2532602663901429 1105 40-41 0.1886876332661771 1105 42-43 0.17745582813204663 1105 44-45 0.23704277188241463 1105 46-47 0.3699103558839525 1105 48-49 0.44951832487834054 1105 50-51 0.4506918219976441 1105 52-53 0.4226569616215343 1105 54-55 0.2628795861857256 1105 56-57 0.2015865121589968 1105 58-59 0.2022886643341053 1105 60-61 0.260385933410511 1105 62-63 0.2422821135993729 1105 64-65 0.3224607184074699 1105 66-67 0.28208292384267963 1105 68-69 0.27350839573722396 1105 70-71 0.2095890974265373 1105 72-73 0.15857617803171564 1105 74-75 0.23175524144692616 1105 76-77 0.20972204993218213 1105 78-79 0.20579185379956755 1105 80-81 0.19881166538123551 1105 82-83 0.1606029548843111 1105 84-85 0.19646573706862824 1105 86-87 0.2575235630976991 1105 88-89 0.2712872734656955 1105 90-91 0.2881407401048648 1105 92-93 0.3194442369265218 1105 94-95 0.2543860096294708 1105 96-97 0.2692837669726771 1105 98-99 0.2723533235965938 1105 100-101 0.2103086448731375 1105 102-103 0.21934838528723333 1105 104-105 0.11364735703257267 1105 106-107 0.10434403544729776 1105 108-109 0.1322006613961122 1105 110-111 0.08077821130492069 1105 112-113 0.06641878268648682 1105 114-115 0.15545562458670759 1105 116-117 0.1529361723821019 1105 118-119 0.15573092534737043 1105 120-121 0.0684920600083494 1105 122-123 0.07090682236026069 1105 124-125 0.2552882263538869 1106 1 0.03153359255659893 1106 2 0.07901438850291953 1106 3 0.06099740721683844 1106 4 0.07135983915914323 1106 5 0.07943260659879314 1106 6 0.10270072940311081 1106 7 0.08137225972395612 1106 8 0.03095854114802421 1106 9 0.08725551320512182 1106 10-11 0.009884407850911714 1106 12-13 0.0160915135690729 1106 14-15 0.05857823509249016 1106 16-17 0.09457625198213293 1106 18-19 0.19630160108953731 1106 20-21 0.13164371196116065 1106 22-23 0.16426041493932075 1106 24-25 0.03355451493968076 1106 26-27 0.13391948398970044 1106 28-29 0.16741961746079426 1106 30-31 0.13453897615313082 1106 32-33 0.1738116747092704 1106 34-35 0.16551573197443759 1106 36-37 0.16650755576480947 1106 38-39 0.16783757393942267 1106 40-41 0.1316157815227541 1106 42-43 0.12021739974064616 1106 44-45 -0.013355581869177513 1106 46-47 -0.038847705836907664 1106 48-49 0.04666321795800599 1106 50-51 0.00189941856583431 1106 52-53 0.07257005572387953 1106 54-55 0.01927343732004516 1106 56-57 0.013851264708549138 1106 58-59 -0.07498237269806651 1106 60-61 -0.11236086503679843 1106 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0.44223080411670423 2206 40-41 0.47967405953308173 2206 42-43 0.47647511528480635 2206 44-45 0.45738723439028206 2206 46-47 0.2970476273335336 2206 48-49 0.4176079492428073 2206 50-51 0.32209762853297264 2206 52-53 0.3352645380085377 2206 54-55 0.3433799247385636 2206 56-57 0.25964341520204925 2206 58-59 0.27585602980433777 2206 60-61 0.23439505307502628 2206 62-63 0.28855890479459845 2206 64-65 0.2958810505490561 2206 66-67 0.2001520496785716 2206 68-69 0.24513755990972186 2206 70-71 0.3514874356282718 2206 72-73 0.31721598072247303 2206 74-75 0.35535264598430416 2206 76-77 0.3368873433064721 2206 78-79 0.34605003712837856 2206 80-81 0.3532773461559131 2206 82-83 0.396668316841442 2206 84-85 0.3783626469154484 2206 86-87 0.3297803311557672 2206 88-89 0.3689401374263426 2206 90-91 0.2905667627688189 2206 92-93 0.33317307192317713 2206 94-95 0.3328206852893203 2206 96-97 0.3281723323918726 2206 98-99 0.4208880604249252 2206 100-101 0.4397196174738056 2206 102-103 0.4598391942049673 2206 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-0.8217814097831706 2216 106-107 -0.8565531674162514 2216 108-109 -0.8142674806315711 2216 110-111 -0.7534609012615725 2216 112-113 -0.7499649483254949 2216 114-115 -0.8295796437371834 2216 116-117 -0.8592980705348765 2216 118-119 -0.7482233634480231 2216 120-121 -0.8464266899270676 2216 122-123 -0.7266878018623011 2216 124-125 -1.3010351788687764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 20.0 4 59.0 5 125.0 6 338.0 7 744.0 8 1046.0 9 1661.0 10 3816.0 11 11827.0 12 17456.0 13 5901.0 14 5046.0 15 6485.0 16 4013.0 17 2683.0 18 2263.0 19 2141.0 20 2206.0 21 2322.0 22 2587.0 23 3072.0 24 3880.0 25 5061.0 26 6831.0 27 8892.0 28 11495.0 29 14944.0 30 19406.0 31 25640.0 32 34380.0 33 47108.0 34 84548.0 35 130639.0 36 262434.0 37 495610.0 38 64757.0 39 231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 72.4394855192713 9.41668237665333 6.089423571496731 12.054408532578634 2 10.963488843813387 31.023489153492406 45.69182292553768 12.321199077156528 3 38.501337800927786 19.920227426157723 18.451898005066305 23.126536767848187 4 16.0283669252008 15.313845347703344 47.10121898094247 21.55656874615338 5 16.931707823296257 32.13949936727781 28.656506970786356 22.272285838639576 6 31.483328120306343 38.48421691560161 14.450665667417137 15.581789296674915 7 47.248217813810314 20.954158640893354 16.74387925107922 15.053744294217108 8 45.47965737431794 21.315860373221685 15.678980422274385 17.52550183018599 9 56.572953505224234 8.795576586006353 12.884462928669196 21.74700698010021 10-11 37.09645327916065 24.953587288413775 22.89636997627881 15.053589456146762 12-13 28.446076093621294 27.571821638930004 27.432157699477887 16.54994456797082 14-15 24.51791166797763 27.239732687555357 25.87963507763581 22.362720566831207 16-17 21.627936504004115 34.82644974885265 23.676173208059012 19.869440539084227 18-19 23.261013631943715 25.314630958943134 26.630754556884412 24.79360085222874 20-21 23.32546497872525 26.139414650158866 24.89858106392335 25.636539307192542 22-23 23.597670306393574 24.173667928080814 25.322643829083546 26.906017936442066 24-25 19.637106014530005 21.363550498888262 38.8536331204826 20.145710366099134 26-27 16.07625620126472 22.74536414817384 34.15000092902842 27.02837872153302 28-29 20.614714881177264 22.675261211824672 24.407550833338494 32.30247307365956 30-31 20.45925745854985 28.579507800741982 23.968430065837147 26.992804674871017 32-33 30.802672814770315 23.091388525879637 21.63072358927034 24.475215070079713 34-35 29.02830594763996 16.195984739159787 29.515387807430987 25.26032150576927 36-37 24.694078682513826 24.10430047256579 31.91557144538242 19.286049399537962 38-39 22.94812460129197 29.7047160579466 25.356437237936564 21.99072210282487 40-41 19.879659851727062 27.646531007871967 28.857867941706562 23.615941198694408 42-43 21.83538080875021 21.18575768461343 25.542242922351804 31.436618584284552 44-45 22.302333719396255 22.44935246718981 25.04885141119417 30.19946240221976 46-47 23.06293703045355 22.072166927826878 24.18888076849231 30.676015273227257 48-49 16.500396385460085 21.799071281254065 25.622603880861394 36.07792845242446 50-51 22.177418106144593 24.213229055054224 25.374901677825328 28.234451160975855 52-53 23.48874172390514 21.93211589319889 30.284894307533182 24.294248075362784 54-55 19.3826296459163 24.38618317963074 30.198146278621817 26.03304089583114 56-57 20.58870208535913 26.78152812788386 27.31900977957252 25.310760007184484 58-59 25.138270396819006 21.486027412531975 31.592385683052665 21.783316507596357 60-61 25.385701633231967 29.308562854966276 27.57666032862832 17.729075183173435 62-63 26.622354591568136 23.68581187793805 26.219775608668456 23.472057921825353 64-65 31.93221653794462 17.86637784205278 28.764848970946183 21.436556649056417 66-67 31.780242971899987 23.922791544602653 23.390148582612305 20.906816900885055 68-69 22.379597916498923 28.748552264042264 25.25617958738751 23.615670232071302 70-71 25.206553985841605 27.223242433063504 22.564358443939327 25.005845137155564 72-73 24.29463517053865 27.662634167187957 23.597747725428746 24.444982936844646 74-75 20.273800159792888 26.846598826946778 28.193651329439675 24.68594968382066 76-77 22.674022507261906 29.254446949380338 22.881002297796964 25.190528245560795 78-79 23.17225270811785 30.065837147511132 18.382607968586452 28.379302175784566 80-81 22.299236957989336 21.75711016419029 24.20475167070278 31.7389012071176 82-83 24.626724121913306 17.689126961024158 28.212967378715337 29.471181538347196 84-85 26.27698827566131 19.307339634210543 31.62734037743328 22.788331712694866 86-87 28.02901975114425 23.637928204683543 23.981862268439667 24.35118977573254 88-89 22.96991805969317 26.68022532035997 26.436703745223245 23.913152874723615 90-91 19.035250435094976 28.198180342997293 25.786887073498537 26.979682148409196 92-93 24.985987154633683 22.133753770307013 26.24156906706966 26.638690007989645 94-95 21.631575198657245 21.21595110833091 29.82874135229377 27.323732340718077 96-97 21.732761877628377 20.880300881338297 28.417663307712793 28.969273933320533 98-99 18.910025145702626 22.70169981233626 30.79562768256957 27.59264735939155 100-101 18.327137229884986 27.049049603924214 28.69761053889842 25.926202627292376 102-103 19.953478901764534 27.191616757195327 25.9628605404468 26.892043800593342 104-105 19.013456976693774 22.76545438780124 23.918649626220898 34.30243900928409 106-107 18.883051921483133 25.009377384265274 22.526160869849647 33.581409824401945 108-109 21.7255231978397 24.448157117286744 25.165792863823018 28.660526821050546 110-111 23.3297139528087 27.81627482517618 21.90441351519269 26.949597706822438 112-113 19.51715939499957 27.61695435012931 23.782998501550516 29.08288775332061 114-115 21.30724651930866 23.38662257874838 25.499211674266054 29.806919227676907 116-117 24.694187323547276 22.002795758282655 24.64177169470877 28.661245223461297 118-119 20.68668381537461 23.692585216110494 26.3602351269798 29.2604958415351 120-121 17.466369066973865 26.105276278424487 27.992874329936175 28.43548032466547 122-123 17.800653124172563 28.232833038359047 29.30999242821905 24.65652140924934 124-125 18.473952341696894 28.3549517868282 24.798564853230843 28.372531018244068 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 10.0 2 57.0 3 147.0 4 230.0 5 288.5 6 357.5 7 462.5 8 590.0 9 772.0 10 966.0 11 1098.5 12 1259.0 13 1432.5 14 1561.5 15 1669.0 16 1736.5 17 1866.0 18 1999.5 19 2014.0 20 2060.5 21 2204.0 22 2343.5 23 2464.5 24 2558.5 25 2653.0 26 2823.0 27 2998.5 28 3139.0 29 3357.0 30 3787.0 31 4114.0 32 4506.5 33 5182.5 34 6027.0 35 6616.0 36 7220.0 37 8162.0 38 8859.0 39 9296.5 40 9701.5 41 10540.5 42 11490.5 43 12503.5 44 13699.5 45 17351.0 46 34581.0 47 78539.5 48 123557.0 49 148495.0 50 146954.5 51 118277.5 52 85690.5 53 60211.5 54 45519.5 55 39029.5 56 34238.0 57 29797.5 58 26312.5 59 21892.0 60 18789.0 61 16719.0 62 13320.5 63 10125.5 64 8190.5 65 7254.5 66 6428.0 67 5730.0 68 5158.0 69 4741.0 70 4061.5 71 2950.0 72 2189.0 73 1741.5 74 1441.0 75 1289.0 76 932.5 77 674.0 78 592.0 79 503.5 80 514.5 81 490.0 82 321.0 83 128.0 84 46.5 85 32.5 86 22.5 87 11.0 88 3.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2077152713692021 2 9.290284220761927E-4 3 0.0 4 0.12216723750301933 5 0.03298050898370484 6 0.002787085266228578 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 3.870951758650803E-5 108-109 0.0 110-111 0.001122576010008733 112-113 2.7096662310555623E-4 114-115 8.128998693166685E-4 116-117 0.005612880050043664 118-119 0.002283861537603974 120-121 3.096761406920642E-4 122-123 0.002632247195882546 124-125 0.029070847707467533 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1291672.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.24315397524455 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14805326430057 27.10278974770302 2 10.553910201826717 7.227983441641131 3 3.348765965147095 3.440169257147712 4 1.6546942212485336 2.266477960006436 5 0.9659169548233325 1.6538021505657357 6 0.6723661714327337 1.381436300167006 7 0.4752008764015294 1.1390663745852105 8 0.3769870305067459 1.0327379945850175 9 0.28589647330309104 0.8810997260667426 >10 2.07082310321061 13.981657803581676 >50 0.23103509773526482 5.53646402985178 >100 0.17479686371528405 12.066157100446793 >500 0.02755196040504191 6.4780542434623865 >1k 0.011517622792271618 7.020933872785109 >5k 0.0013550144461496022 2.934643329563922 >10k+ 0.0011291787051246685 5.8565266678403445 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 17363 1.344226707709078 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 16994 1.315659083730235 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 15350 1.1883821899057965 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 14890 1.152769433726209 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 11134 0.8619835376163608 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 9451 0.7316873014201748 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 7203 0.5576493103512348 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 5581 0.43207563530060267 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 5543 0.42913371196402805 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 5145 0.3983209359651676 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 5025 0.3890306517444057 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 4685 0.36270817978558023 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 3275 0.25354734019162756 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 3143 0.24332802754878946 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 3036 0.23504419078527677 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 3005 0.23264420069491323 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2969 0.2298571154286847 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2739 0.212050737338891 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 2626 0.20330238636434017 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2591 0.2005927201332846 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 2516 0.1947862924953084 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 2501 0.19362500696771318 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2346 0.18162505651589567 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2178 0.16861865860682898 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 2154 0.1667606017626766 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 2064 0.15979288859710514 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 2037 0.1577025746474337 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1959 0.15166388990393848 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1944 0.15050260437634322 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1940 0.15019292823565117 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1875 0.14516069094940512 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1818 0.1407478059445432 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1798 0.13919942524108286 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1781 0.1378833016431416 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1747 0.13525105444725907 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1556 0.12046401872921299 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGT 1519 0.1175995144278114 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1516 0.11736725732229235 No Hit GTACAGGGGATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1510 0.11690274311125426 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 1487 0.11512210530227487 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 1324 0.10250280256907326 No Hit GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG 1295 0.1002576505490558 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.741903517301606E-5 0.0 5 0.0 0.0 0.0 7.741903517301606E-5 0.0 6 0.0 0.0 0.0 7.741903517301606E-5 0.0 7 0.0 0.0 0.0 7.741903517301606E-5 0.0 8 0.0 0.0 0.0 7.741903517301606E-5 0.0 9 0.0 0.0 0.0 7.741903517301606E-5 0.0 10-11 0.0 0.0 0.0 7.741903517301606E-5 0.0 12-13 0.0 0.0 0.0 7.741903517301606E-5 0.0 14-15 0.0 0.0 0.0 7.741903517301606E-5 0.0 16-17 0.0 0.0 0.0 1.1612855275952409E-4 0.0 18-19 0.0 0.0 0.0 1.548380703460321E-4 0.0 20-21 0.0 0.0 0.0 7.741903517301605E-4 0.0 22-23 0.0 0.0 0.0 0.0017032187738063532 0.0 24-25 7.741903517301606E-5 0.0 0.0 0.002864504301401594 0.0 26-27 7.741903517301606E-5 0.0 0.0 0.0049161087334865195 0.0 28-29 1.1612855275952409E-4 0.0 0.0 0.011883821899057966 0.0 30-31 2.3225710551904818E-4 0.0 0.0 0.04134176478239057 0.0 32-33 2.3225710551904818E-4 0.0 0.0 0.09750927480041373 0.0 34-35 2.3225710551904818E-4 0.0 0.0 0.16188320254677657 0.0 36-37 2.3225710551904818E-4 0.0 0.0 0.240928037458426 0.0 38-39 2.3225710551904818E-4 0.0 0.0 0.36805009321251836 0.0 40-41 2.3225710551904818E-4 0.0 0.0 0.5751847218179228 0.0 42-43 3.096761406920643E-4 0.0 0.0 1.0697375185031492 0.0 44-45 3.870951758650803E-4 0.0 0.0 1.7571024222867724 0.0 46-47 3.870951758650803E-4 0.0 0.0 2.4457447401507504 0.0 48-49 3.870951758650803E-4 0.0 0.0 2.6885308344533287 0.0 50-51 3.870951758650803E-4 0.0 0.0 3.011832725335844 0.0 52-53 3.870951758650803E-4 0.0 0.0 3.268399407899219 0.0 54-55 4.6451421103809636E-4 0.0 0.0 3.5172241869452927 0.0 56-57 4.6451421103809636E-4 0.0 0.0 3.945157903864139 0.0 58-59 4.6451421103809636E-4 0.0 0.0 4.444084876036641 0.0 60-61 4.6451421103809636E-4 0.0 0.0 5.206468824902917 0.0 62-63 4.6451421103809636E-4 0.0 0.0 6.073562018840697 0.0 64-65 4.6451421103809636E-4 0.0 0.0 6.791004217789036 0.0 66-67 4.6451421103809636E-4 0.0 0.0 7.350782551607529 0.0 68-69 4.6451421103809636E-4 0.0 0.0 7.822458023399129 0.0 70-71 4.6451421103809636E-4 0.0 0.0 8.3791395958107 0.0 72-73 4.6451421103809636E-4 0.0 0.0 8.911163205519667 0.0 74-75 4.6451421103809636E-4 0.0 0.0 9.458167398534613 0.0 76-77 4.6451421103809636E-4 0.0 0.0 10.120022730228726 0.0 78-79 4.6451421103809636E-4 0.0 0.0 11.010612601341517 0.0 80-81 4.6451421103809636E-4 0.0 0.0 13.784923726766547 0.0 82-83 4.6451421103809636E-4 0.0 0.0 15.636167695823708 0.0 84-85 4.6451421103809636E-4 0.0 0.0 16.279790844734578 0.0 86-87 4.6451421103809636E-4 0.0 0.0 16.790485510253376 0.0 88-89 4.6451421103809636E-4 0.0 0.0 17.427799007797645 0.0 90-91 4.6451421103809636E-4 0.0 0.0 18.339988789723705 0.0 92-93 4.6451421103809636E-4 0.0 0.0 19.557983760583184 0.0 94-95 5.032237286246044E-4 0.0 0.0 20.990932682600537 0.0 96-97 5.419332462111125E-4 0.0 0.0 22.481442657269028 0.0 98-99 6.193522813841284E-4 0.0 0.0 23.92848184368787 0.0 100-101 6.193522813841284E-4 0.0 0.0 25.28734074904465 0.0 102-103 6.967713165571445E-4 0.0 0.0 26.475916486538377 0.0 104-105 7.741903517301606E-4 0.0 0.0 27.64595036510817 0.0 106-107 7.741903517301606E-4 0.0 0.0 28.78261663951839 0.0 108-109 7.741903517301606E-4 0.0 0.0 29.696432221183088 0.0 110-111 7.741903517301606E-4 0.0 0.0 30.74449240983779 0.0 112-113 7.741903517301606E-4 0.0 0.0 34.917300986628184 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGTT 25 1.7113389E-5 95.36144 4 GAGTAGT 2785 0.0 92.44487 1 AGTAGTA 2360 0.0 91.169174 2 GAGTCGT 855 0.0 85.93494 1 GAGTACG 605 0.0 85.90029 1 GAGTCCG 670 0.0 85.59083 1 GAGTTCG 400 0.0 85.12274 1 GAGTTAG 1270 0.0 84.193794 1 GAGTCAG 6070 0.0 83.74745 1 GAGTAAG 1080 0.0 82.965645 1 GTATACG 175 0.0 81.92264 1 AGTACGG 345 0.0 81.195435 2 AGTCCGT 370 0.0 80.54177 2 GAGTCGG 745 0.0 80.18156 1 TCGTAGG 1815 0.0 78.482735 2 GAGTGGT 1015 0.0 76.50821 1 AGTCGTA 570 0.0 75.285355 2 GAGTAAC 855 0.0 74.75641 1 AGTCGGT 385 0.0 74.30762 2 GTTAACG 130 0.0 73.52032 1 >>END_MODULE