FastQCFastQC Report
Thu 26 May 2016
SRR935974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4030813
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT57940.14374271393885055No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT52130.12932874831950775No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC40570.1006496704262887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81800.059.9524731
GTATCAA134950.050.1168251
TATCAAC173150.038.7619322
ATCAACG185150.035.8305783
TCAACGC187350.035.378074
GAGTAAG21600.035.0356751
GATTGTG50250.033.9739579
CAACGCA197700.033.4298635
AACGCAG199600.033.079796
GAGTAGT22500.032.045231
CGGGATT15500.030.7014276
GTAAGGG16200.029.0144773
GTAGGGG29250.028.4776953
AGTAGTA20150.027.4616582
ACGCAGA239800.027.4102757
TAAGGGG18950.027.3157224
GGGGTAA57400.026.9439819
AGGGTAA39400.026.873496
TAGGGGG31450.026.863984
GGGATTG62300.026.734397
Produced by FastQC (version 0.11.5)