FastQCFastQC Report
Thu 26 May 2016
SRR935974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4030813
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT57580.14284959386605134No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT56730.14074083813860877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99750.070.115051
GTATCAA169600.050.7111971
TATCAAC219950.038.9648442
ATCAACG234000.036.547973
TCAACGC235900.036.228344
CAACGCA246850.034.478025
AACGCAG250800.034.018536
GATTGTG48900.031.876479
GTGGTAT43150.031.6972731
AGGGTAA37700.031.2476796
GATAGCG10050.030.783299
TGGTATC41150.030.4149762
CGGGATT14050.029.6425576
GTATAGG15400.029.4754281
GTACGGG12150.029.0030061
GAGTAAG22750.028.6162221
GAGTAGT22850.028.490991
ACGCAGA302750.028.101517
GGGATTG58800.027.8248717
TATAGGG12400.027.3962542
Produced by FastQC (version 0.11.5)