FastQCFastQC Report
Thu 26 May 2016
SRR935973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115627
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC151820.48728554477156605No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT139610.44809600122222587No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT136470.4380177729875881No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT127650.4097088643794652No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG112450.36092253661943485No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT105310.3380058010795259No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA76470.24544016340852098No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA56120.18012425749295405No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC48610.1560199600273075No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC44880.14404805196514217No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC41630.13361676477960938No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG38450.1234101514719188No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT35660.1144552926264922No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT34220.10983342999659458No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG33950.10896683075348877No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG32730.10505108602538109No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC32580.10456964200143341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTA28450.062.3251272
GAGTAGT35450.061.352691
GGTATCA80250.060.8129231
GAGTAAG21800.057.674391
GATTGTG115100.057.4209379
GAGTTAG20250.054.7324561
GAGTCAG89350.053.2188341
AGGGTAA93950.050.846066
GATAGCG24700.050.57719
GTATCAA137350.049.804691
GTGGGGT99700.049.345657
CGGGATT34450.049.2146226
TATCGGG5500.048.683182
AGTCGTA8050.048.0448272
GTAGTAG55700.047.8199771
TAGTAGG55300.047.665892
GGGATTG140900.047.498527
TGGGGTA106850.047.1563768
GGGGTAA121900.046.7512639
GAGTACG12300.046.0230641
Produced by FastQC (version 0.11.5)