FastQCFastQC Report
Thu 26 May 2016
SRR935973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115627
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT141160.45307092280301847No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT140300.45031064373238516No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT131630.42248317914820993No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC124620.39998369509572235No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG121310.38935983030061044No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT116610.37427458421691684No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA62950.20204600871670453No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA53900.17299888593852858No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC48410.15537803466204395No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC41190.13220452897602955No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG41190.13220452897602955No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT36460.11702299408754642No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG35800.1149046403821767No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC35060.11252951653070152No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT34780.11163082101933255No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT34330.11018648894748953No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG33550.10768298002296169No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC33010.10594978153675007No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG31380.10071808980985208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91000.067.854621
GATTGTG118750.058.818619
CGGGATT33450.056.5620656
AGTAGTA24950.056.365362
AGGGTAA93050.056.3318186
TAGTAGG52350.056.231692
GTAGTAG53850.055.2260441
GAGTAGT33700.054.2240331
AGTCGTA7400.052.342262
GTATCAA155300.049.0274161
GGGATTG144700.048.763687
TCGTAGG16050.048.6370352
GAGTAAG21300.048.5027961
GATAGCG24200.048.431869
GAGTTAG22450.047.8802571
GTATACG2250.047.7738531
GAGTCAG82600.046.7761541
GAGTACG13350.046.5213551
ACGGGAT11150.046.439985
AGTAGGG64550.045.2338643
Produced by FastQC (version 0.11.5)