FastQCFastQC Report
Thu 26 May 2016
SRR935972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167697
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC106380.9110240070840295No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT97660.8363471003179763No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG89130.7632973279883395No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT87070.7456557651514049No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT65340.5595629688181095No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA62460.5348990363082203No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT40710.34863496266582855No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC34460.29511080357318725No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC32200.2757564676452881No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG29540.25297658553546No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG29310.2510068964808508No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG26620.22797009840737795No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG25310.21675143466156033No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA19890.17033528389642175No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC19880.17024964524187353No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA19700.16870814946000548No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA19370.165882073859914No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC18510.15851714956876656No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA18020.1543208554959035No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14530.12443296505857257No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG14510.1242616877494761No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT14460.12383349447673496No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC14290.12237763734941513No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG13680.11715367942197333No Hit
GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA13480.11544090633100881No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT12510.10713395683983089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT21050.092.824811
AGTAGTA18000.089.5737462
AGTCCGT2550.088.6599352
GTCGCAA304.2387364E-579.429351
GAGTCCG7500.078.635071
GAGTTAG11650.077.724871
GAGTAAG12650.077.702641
GAGTCAG50350.077.1419141
GAGTACG5450.072.1422651
GAGTCGT6200.072.062931
GAGTCGG8050.069.562361
GTGGGGT58900.068.8836067
TGGGGTA61950.068.754318
GAGTTAC6550.068.2122341
TAAGCGG1700.066.4920964
TCGTAGG8550.065.410242
GAGTCGC4100.065.383931
GAGTAAC11000.064.9876561
AGTAAGG7200.064.453442
AGTTAGT8600.064.3381352
Produced by FastQC (version 0.11.5)