##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935972_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1167697 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.607595120994574 31.0 31.0 34.0 26.0 34.0 2 30.684329068242874 33.0 31.0 34.0 26.0 34.0 3 30.759734759959134 34.0 31.0 34.0 26.0 34.0 4 33.88201562562891 37.0 35.0 37.0 28.0 37.0 5 34.03597679877571 37.0 35.0 37.0 30.0 37.0 6 34.14190153781332 37.0 35.0 37.0 30.0 37.0 7 33.981861732966685 37.0 35.0 37.0 30.0 37.0 8 33.92723454800346 37.0 35.0 37.0 29.0 37.0 9 35.42019462240633 39.0 37.0 39.0 27.0 39.0 10-11 35.24741949324182 39.0 37.0 39.0 27.0 39.0 12-13 35.11399832319515 39.0 37.0 39.0 27.0 39.0 14-15 36.54522577346692 40.0 37.0 41.0 27.0 41.0 16-17 36.74856062831368 40.0 37.0 41.0 27.5 41.0 18-19 36.87919254738172 40.0 37.0 41.0 28.5 41.0 20-21 37.03441260875039 40.0 37.0 41.0 29.0 41.0 22-23 36.95543407236637 40.0 37.0 41.0 29.0 41.0 24-25 36.94976907536801 40.0 37.0 41.0 29.0 41.0 26-27 36.95363009410832 40.0 37.0 41.0 30.0 41.0 28-29 36.86007757149329 40.0 36.0 41.0 29.5 41.0 30-31 36.78768850138349 40.0 36.0 41.0 29.0 41.0 32-33 36.714293605276026 40.0 36.0 41.0 29.0 41.0 34-35 36.50663870850058 40.0 35.5 41.0 29.0 41.0 36-37 36.501192946457856 40.0 35.5 41.0 29.5 41.0 38-39 36.61685180316469 40.0 35.5 41.0 30.0 41.0 40-41 36.57566945877227 40.0 35.0 41.0 30.0 41.0 42-43 36.41607583131583 40.0 35.0 41.0 30.0 41.0 44-45 36.24457500533101 40.0 35.0 41.0 30.0 41.0 46-47 36.088950301319606 40.0 35.0 41.0 30.0 41.0 48-49 35.92993773213428 39.5 35.0 41.0 29.0 41.0 50-51 35.708823864410036 39.0 35.0 41.0 29.0 41.0 52-53 35.471024589426875 39.0 35.0 41.0 29.0 41.0 54-55 35.20722327795652 38.5 35.0 41.0 28.0 41.0 56-57 34.929312569956075 38.0 34.5 40.0 28.0 41.0 58-59 34.55944435928156 37.0 34.0 40.0 27.0 41.0 60-61 34.2111476693012 37.0 34.0 39.5 27.0 41.0 62-63 33.768030148231944 36.0 34.0 39.0 26.0 41.0 64-65 33.32929689808229 35.5 33.0 39.0 25.5 41.0 66-67 32.99589919302696 35.0 33.0 37.5 25.5 40.0 68-69 32.772819489987555 35.0 33.0 37.0 25.5 40.0 70-71 32.55321842909591 35.0 33.0 37.0 25.0 39.0 72-73 32.28362280625882 35.0 33.0 36.0 25.0 39.0 74-75 31.97370807666715 35.0 33.0 35.5 24.0 38.5 76-77 31.759009400555108 35.0 33.0 35.0 24.0 37.0 78-79 31.549299176070505 35.0 33.0 35.0 23.5 37.0 80-81 31.335518118141948 35.0 33.0 35.0 22.5 36.0 82-83 31.171374508969365 35.0 33.0 35.0 21.0 36.0 84-85 30.923109334013873 35.0 33.0 35.0 19.5 36.0 86-87 30.715844093116623 35.0 32.0 35.0 17.5 35.0 88-89 30.535021499584225 35.0 32.0 35.0 11.5 35.0 90-91 30.404199462703083 35.0 32.0 35.0 7.0 35.0 92-93 30.269801155608004 35.0 32.0 35.0 4.5 36.0 94-95 30.082700820503952 35.0 31.5 35.0 2.0 35.0 96-97 29.95534115442619 35.0 31.0 35.0 2.0 35.0 98-99 29.779125064121942 35.0 31.0 35.0 2.0 35.0 100-101 29.614629908272438 35.0 31.0 35.0 2.0 35.0 102-103 28.897076895804304 34.0 29.5 35.0 2.0 35.0 104-105 29.332072875069475 35.0 30.5 35.0 2.0 35.0 106-107 29.29055011702522 35.0 30.5 35.0 2.0 35.0 108-109 29.136186870395317 35.0 30.0 35.0 2.0 35.0 110-111 28.991096149086623 35.0 30.0 35.0 2.0 35.0 112-113 28.846678975795946 35.0 30.0 35.0 2.0 35.0 114-115 28.615247791165004 35.0 29.5 35.0 2.0 35.0 116-117 28.39077731637574 35.0 29.0 35.0 2.0 35.0 118-119 28.10952541626809 35.0 29.0 35.0 2.0 35.0 120-121 27.76732448571847 35.0 29.0 35.0 2.0 35.0 122-123 27.252103927645614 34.5 27.5 35.0 2.0 35.0 124-125 24.55931418852665 31.5 20.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4182593411452231 1101 2 0.5834334128286045 1101 3 0.49338735396656475 1101 4 0.6086637291188026 1101 5 0.5704859004469114 1101 6 0.5542308512982572 1101 7 0.6101673352688621 1101 8 0.5154484346933685 1101 9 0.5978759386441865 1101 10-11 0.7461215066852205 1101 12-13 0.4769379877190687 1101 14-15 0.6406276060395371 1101 16-17 0.5346878294469306 1101 18-19 0.5432127885114255 1101 20-21 0.496017510629045 1101 22-23 0.5927557983975618 1101 24-25 0.6602172139821576 1101 26-27 0.5760437186264866 1101 28-29 0.6049890418831438 1101 30-31 0.4536853488706285 1101 32-33 0.4208179119456972 1101 34-35 0.23803302604765264 1101 36-37 0.29113515438147175 1101 38-39 0.393535937870098 1101 40-41 0.4997306874279914 1101 42-43 0.34602170144509614 1101 44-45 0.41727315273813304 1101 46-47 0.48882107353422555 1101 48-49 0.449777288029388 1101 50-51 0.48732953253637845 1101 52-53 0.4413502885411731 1101 54-55 0.34953579007579805 1101 56-57 0.28225157760566333 1101 58-59 0.26871273315875044 1101 60-61 0.37558943031898906 1101 62-63 0.5131271434464182 1101 64-65 0.47247825543297495 1101 66-67 0.4796543134757911 1101 68-69 0.493925815137942 1101 70-71 0.4641606084015706 1101 72-73 0.5978640979983396 1101 74-75 0.5182188846874354 1101 76-77 0.6314008185330628 1101 78-79 0.5637922970783329 1101 80-81 0.6838939407073354 1101 82-83 0.5610405050429712 1101 84-85 0.44235172219619656 1101 86-87 0.36270986510978176 1101 88-89 0.336676401525974 1101 90-91 0.4133451622663529 1101 92-93 0.27729474850375624 1101 94-95 0.22576961932320927 1101 96-97 0.2920013713683076 1101 98-99 0.2663415858666376 1101 100-101 0.3719715135760815 1101 102-103 0.2765239709130256 1101 104-105 0.26450340223964375 1101 106-107 0.3079417903916237 1101 108-109 0.1901054922122647 1101 110-111 0.1996870575078198 1101 112-113 0.3038667401449473 1101 114-115 0.15658281921992412 1101 116-117 0.2858180229828733 1101 118-119 0.28797876472591355 1101 120-121 0.09493562156955804 1101 122-123 0.053368943916076717 1101 124-125 0.06311563575062706 1102 1 0.14388053195628459 1102 2 0.2663262990526043 1102 3 0.23186622813275903 1102 4 0.22611875909881718 1102 5 0.17271198840473545 1102 6 0.2288305009145617 1102 7 0.32821620803681384 1102 8 0.28399944184086934 1102 9 0.25545401416585634 1102 10-11 0.22999007989979248 1102 12-13 0.1606066941781421 1102 14-15 0.27508344206655266 1102 16-17 0.3381295678764573 1102 18-19 0.22782263708973716 1102 20-21 0.32314510457875656 1102 22-23 0.41035209222636837 1102 24-25 0.4344226819417898 1102 26-27 0.49388840653002575 1102 28-29 0.35127526259196884 1102 30-31 0.35559868236845915 1102 32-33 0.5322646018067729 1102 34-35 0.41064305557528513 1102 36-37 0.3629319400634472 1102 38-39 0.35541012051376697 1102 40-41 0.3171252638831916 1102 42-43 0.1453410852303847 1102 44-45 0.2398469924005724 1102 46-47 0.17546043330261085 1102 48-49 0.20843324578594746 1102 50-51 0.09398065401095579 1102 52-53 0.31018855274363943 1102 54-55 0.296976796483051 1102 56-57 0.28468370104599927 1102 58-59 0.08367518617360759 1102 60-61 0.013938144345033265 1102 62-63 0.10859909218840613 1102 64-65 0.07490379889782162 1102 66-67 -0.059789143972324155 1102 68-69 0.10657849113172091 1102 70-71 0.19790395645760128 1102 72-73 0.1736417688515175 1102 74-75 0.30504577375726427 1102 76-77 0.20134287362390424 1102 78-79 0.2364025322302048 1102 80-81 0.11518994912970015 1102 82-83 0.04306318950847299 1102 84-85 0.07580801882149402 1102 86-87 0.2171168036939335 1102 88-89 0.18949550977479745 1102 90-91 0.23967349481831235 1102 92-93 0.295000023176609 1102 94-95 0.2191483133625738 1102 96-97 0.33304108974535396 1102 98-99 0.20728301585981157 1102 100-101 0.2872236911367203 1102 102-103 0.27903011907762476 1102 104-105 0.11605833269753063 1102 106-107 0.19598638617550534 1102 108-109 0.32672963578733416 1102 110-111 0.3824004337754978 1102 112-113 0.2744147698535393 1102 114-115 0.18568666768308972 1102 116-117 0.17954833446246 1102 118-119 0.1542167048645844 1102 120-121 -0.09760803245533012 1102 122-123 -0.22040841438826675 1102 124-125 -0.27929477377059797 1103 1 0.09925100030282152 1103 2 0.24268707828774083 1103 3 0.16155502366644825 1103 4 0.1555659190915506 1103 5 0.15363186174349153 1103 6 0.2841658294520357 1103 7 0.28064954110692497 1103 8 0.17445483797311567 1103 9 0.11126234822304326 1103 10-11 0.32851716913911133 1103 12-13 0.3654865871004347 1103 14-15 0.3667535305872818 1103 16-17 0.409267939758287 1103 18-19 0.40782255812387547 1103 20-21 0.32505213017159207 1103 22-23 0.3133229854092434 1103 24-25 0.332754330670042 1103 26-27 0.3751468460066434 1103 28-29 0.23959127607934505 1103 30-31 0.416833353120289 1103 32-33 0.2890450056381937 1103 34-35 0.37039652415082713 1103 36-37 0.3715421803565633 1103 38-39 0.36238576744159445 1103 40-41 0.3194504137209151 1103 42-43 0.3497217164835007 1103 44-45 0.2939626931831256 1103 46-47 0.34338449972869256 1103 48-49 0.27993249166195966 1103 50-51 0.23403253458297968 1103 52-53 0.2975790863159702 1103 54-55 0.28619775876105535 1103 56-57 0.271287932036941 1103 58-59 0.1820536221757294 1103 60-61 0.22064096421600254 1103 62-63 0.10747955366490913 1103 64-65 0.17099735765237511 1103 66-67 0.2666539571750377 1103 68-69 0.43281233274199593 1103 70-71 0.3397996899320219 1103 72-73 0.19074083942330589 1103 74-75 0.22956704321248367 1103 76-77 0.25698913024715964 1103 78-79 0.20716443435991394 1103 80-81 0.2801867510122449 1103 82-83 0.29673628662858675 1103 84-85 0.2883584582665186 1103 86-87 0.33854221648798344 1103 88-89 0.2626242162349932 1103 90-91 0.18511183432414668 1103 92-93 0.25633340125726534 1103 94-95 0.4165544821757976 1103 96-97 0.24173641575591986 1103 98-99 0.3173282632993413 1103 100-101 0.24907390079108538 1103 102-103 0.2723385518421395 1103 104-105 0.23266880199165385 1103 106-107 0.09579489395758856 1103 108-109 0.09559418564242961 1103 110-111 0.2471510595799593 1103 112-113 0.3550712920389998 1103 114-115 0.3729299299208222 1103 116-117 0.37374019781993084 1103 118-119 0.3194742915098807 1103 120-121 0.20568050091169354 1103 122-123 0.0852852217528337 1103 124-125 0.03172570207769354 1104 1 0.0846327791386372 1104 2 0.10614498265857719 1104 3 0.2335622148115526 1104 4 0.1861366440492489 1104 5 0.3083022688592578 1104 6 0.13542788909507664 1104 7 0.03312377676282097 1104 8 0.07669128106132916 1104 9 0.08017379091233323 1104 10-11 0.05369958821783172 1104 12-13 0.08942343510847195 1104 14-15 0.1718483474767467 1104 16-17 0.24050626193587732 1104 18-19 0.1656230965479466 1104 20-21 0.20818804387785406 1104 22-23 0.2553493169754404 1104 24-25 0.08747275980299207 1104 26-27 0.19798074529565923 1104 28-29 0.16031448613843224 1104 30-31 0.276118964827333 1104 32-33 0.3533040874602662 1104 34-35 0.39481024902570283 1104 36-37 0.30328139040958746 1104 38-39 0.09779167126231414 1104 40-41 0.22882063383956108 1104 42-43 0.10389039989759397 1104 44-45 -0.03806586472025231 1104 46-47 0.2144187891206144 1104 48-49 0.32355065431830354 1104 50-51 0.2789935280916538 1104 52-53 0.2544981319847608 1104 54-55 0.2972207872938881 1104 56-57 0.31162984575992425 1104 58-59 0.3368775173843943 1104 60-61 0.37919701358445934 1104 62-63 0.29431464438625454 1104 64-65 0.31880981039969924 1104 66-67 0.18440865432859965 1104 68-69 0.2826515881575844 1104 70-71 0.10942356052180457 1104 72-73 0.07556170286221686 1104 74-75 0.13604378482743584 1104 76-77 0.25020848980669896 1104 78-79 0.18270720852801858 1104 80-81 0.20725350237596984 1104 82-83 0.26711536422593696 1104 84-85 0.2004052219852781 1104 86-87 0.2566625876252324 1104 88-89 0.3357373574751925 1104 90-91 0.3255532493627875 1104 92-93 0.32140152489572316 1104 94-95 0.38438713912307065 1104 96-97 0.3719277515810262 1104 98-99 0.41613375361401594 1104 100-101 0.4054767477672563 1104 102-103 0.6173093093210724 1104 104-105 0.3842346844760023 1104 106-107 0.4085682946230591 1104 108-109 0.4769539685707578 1104 110-111 0.4040894052366646 1104 112-113 0.39116340920968184 1104 114-115 0.44332207680132996 1104 116-117 0.4821605393993238 1104 118-119 0.6329486464609708 1104 120-121 0.7606013139730727 1104 122-123 0.7319208691344556 1104 124-125 0.8639181959020767 1105 1 0.1058945329520462 1105 2 0.22324758871328854 1105 3 0.12056736420493408 1105 4 0.21245270095243995 1105 5 0.23019829669821235 1105 6 0.27987076886465445 1105 7 0.030904761317543716 1105 8 0.10366571120916035 1105 9 0.18006287643407148 1105 10-11 0.2812419469506793 1105 12-13 0.29445915566785175 1105 14-15 0.160630959454636 1105 16-17 0.3784314643475355 1105 18-19 0.38188564157066196 1105 20-21 0.2483687735034863 1105 22-23 0.3329517059920022 1105 24-25 0.2826118311700725 1105 26-27 0.4104146357661591 1105 28-29 0.2905304790190044 1105 30-31 0.2851369361666798 1105 32-33 0.3129212002286508 1105 34-35 0.3357482893526935 1105 36-37 0.26128704937078595 1105 38-39 0.31087812793671077 1105 40-41 0.26199407998434054 1105 42-43 0.33306381120011963 1105 44-45 0.2970903954874302 1105 46-47 0.35518690091776506 1105 48-49 0.3073243307122482 1105 50-51 0.40927085784010586 1105 52-53 0.4098705883194427 1105 54-55 0.4180624017092853 1105 56-57 0.402191735022555 1105 58-59 0.19709079547341446 1105 60-61 0.16376124598803443 1105 62-63 0.14608230758950214 1105 64-65 0.10153284794657225 1105 66-67 0.16517464655587588 1105 68-69 0.25315254704518253 1105 70-71 0.16468553790627993 1105 72-73 0.33298418975645205 1105 74-75 0.29630722123090436 1105 76-77 0.24283100747824804 1105 78-79 0.25211404835096474 1105 80-81 0.2336152897250443 1105 82-83 0.32720117777056856 1105 84-85 0.3561598541890909 1105 86-87 0.3053223226209276 1105 88-89 0.3776097506583085 1105 90-91 0.3325685175246704 1105 92-93 0.3455442967995168 1105 94-95 0.32496332822354645 1105 96-97 0.23434915691392177 1105 98-99 0.29777501205781576 1105 100-101 0.38175643807777604 1105 102-103 0.3017485451725861 1105 104-105 0.2436378803808772 1105 106-107 0.3006792845928494 1105 108-109 0.2553456796007936 1105 110-111 0.29277859644654924 1105 112-113 0.2091366358483171 1105 114-115 0.3440997121453009 1105 116-117 0.15583678816714297 1105 118-119 0.06008226211326928 1105 120-121 0.04076344655071651 1105 122-123 0.2071745279330628 1105 124-125 0.25979033357539905 1106 1 -0.269627139359887 1106 2 -0.19289522364248057 1106 3 -0.1585218240425732 1106 4 -1.0114762517648614 1106 5 -0.45137683423917707 1106 6 -0.38383291777096673 1106 7 -0.39470653005577816 1106 8 -0.4965512318556762 1106 9 -0.45256879585691223 1106 10-11 -0.31208110131234434 1106 12-13 -0.3157017676284255 1106 14-15 -0.24685210997900953 1106 16-17 -0.216850669863895 1106 18-19 -0.19606967528002883 1106 20-21 -0.16755217319778382 1106 22-23 -0.1292745753301574 1106 24-25 -0.157876154569081 1106 26-27 -0.035174165307452654 1106 28-29 -0.021467093899914858 1106 30-31 -0.16545998892546976 1106 32-33 -0.08224823810038373 1106 34-35 -0.032415682457973105 1106 36-37 -0.11482254074157794 1106 38-39 -0.09127719827039016 1106 40-41 -0.03477977345375649 1106 42-43 -0.06752885463005498 1106 44-45 0.0016316764639583425 1106 46-47 0.08560407118979185 1106 48-49 0.040910461717366786 1106 50-51 -0.049191911671677246 1106 52-53 -0.026851965450248372 1106 54-55 -0.017493219015307204 1106 56-57 -0.014802406048389116 1106 58-59 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76-77 -0.7181810669374649 2216 78-79 -0.9689986174059477 2216 80-81 -0.9458783563799926 2216 82-83 -0.9649475730427426 2216 84-85 -0.9361958948334959 2216 86-87 -0.9459052717662253 2216 88-89 -0.8405631950500059 2216 90-91 -0.8937133455167938 2216 92-93 -0.8066586938783651 2216 94-95 -0.8377173993506872 2216 96-97 -0.867212686320201 2216 98-99 -0.7266648836081764 2216 100-101 -0.8250324698123386 2216 102-103 -0.8064251490688932 2216 104-105 -1.2438970271752758 2216 106-107 -0.9676013347319739 2216 108-109 -1.0845802156160822 2216 110-111 -1.2458252740456999 2216 112-113 -1.3570748650758304 2216 114-115 -1.5176536979990516 2216 116-117 -1.3622100584929626 2216 118-119 -1.5708016403931673 2216 120-121 -1.3990878832472369 2216 122-123 -1.1450843148346692 2216 124-125 -1.14213339665325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24855.0 3 4199.0 4 2504.0 5 2038.0 6 2493.0 7 2955.0 8 3801.0 9 4254.0 10 5682.0 11 7236.0 12 6850.0 13 5313.0 14 4994.0 15 4832.0 16 4630.0 17 4471.0 18 4001.0 19 3885.0 20 3793.0 21 4003.0 22 4889.0 23 6610.0 24 10058.0 25 14892.0 26 18169.0 27 15032.0 28 16144.0 29 21021.0 30 23529.0 31 28731.0 32 39256.0 33 52728.0 34 81613.0 35 107095.0 36 211481.0 37 341494.0 38 67915.0 39 251.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 66.88788757620284 10.926631686485624 7.448857139671111 14.736623597640424 2 11.643892523976877 31.3698147889598 42.36230373308642 14.623988953976902 3 36.21409821042399 20.746663012431537 19.816460330851022 23.22277844629345 4 14.14218587528715 16.453421543553226 45.78120690629834 23.623185674861286 5 16.484706994685087 33.89331465206421 28.19935289271345 21.422625460537244 6 31.038360122531788 38.30017547360317 14.987278377866861 15.674186025998182 7 42.028940826056164 25.48949081650742 16.29597848064517 16.185589876791244 8 40.07974705658684 26.199717048673794 15.69891718021533 18.021618714524035 9 52.37103460914946 12.364252027709242 11.253261762255105 24.01145160088619 10-11 38.2361177600011 25.96405574391302 19.502918993540277 16.29690750254561 12-13 28.788204474277144 28.76636661736735 23.30720212520885 19.138226783146656 14-15 25.261947234599386 28.551071039833108 22.178955670863246 24.00802605470426 16-17 23.692961444621336 32.65243466412947 22.037951626149592 21.616652265099592 18-19 24.12839118367179 26.717504626628312 24.88869115875094 24.265413030948952 20-21 24.93039718351593 27.629898852185114 22.535255293111142 24.90444867118782 22-23 25.558899269245362 24.72803304281847 22.994064384853264 26.719003303082907 24-25 22.93407450734223 23.59546183641818 31.46094406340001 22.00951959283958 26-27 19.839648809997882 23.476967714807138 30.120571694298214 26.562811780896766 28-29 22.44893939101967 24.936504775791523 23.55843038007207 29.05612545311674 30-31 22.922470469650946 27.979047646778234 22.174245544863094 26.92423633870773 32-33 28.62583358525371 25.327931817928796 20.85425414298401 25.191980453833484 34-35 27.51407257190864 21.04801159889937 25.756382006633572 25.68153382255842 36-37 24.28857828700425 26.519593695967362 27.657346040967816 21.534481976060572 38-39 23.17707418962282 29.88652021885815 24.10732407465293 22.829081516866104 40-41 21.924822963491387 28.97541057311957 25.397898598694695 23.70186786469435 42-43 22.37052934108763 23.923115328719692 23.533373811870717 30.17298151832196 44-45 23.3519483222103 24.67365249718035 23.13738067323972 28.83701850736963 46-47 24.22683281707498 23.29645447406305 23.91142565237386 28.565287056488113 48-49 19.17616470711152 23.76673914551463 25.80468220779877 31.25241393957508 50-51 23.022196682872355 25.55269046679061 24.854221600295283 26.57089125004175 52-53 24.287079610549654 23.384833565556818 27.618337633821106 24.709749190072426 54-55 21.050409481226723 24.8111881763848 27.9154181264489 26.222984215939583 56-57 20.877465975616857 26.775767925103537 25.968705788855278 26.378060310424324 58-59 22.86132447030351 24.645092005888515 29.59487778079416 22.89870574301381 60-61 24.553886838794654 29.076250088850102 26.691727391609295 19.67813568074595 62-63 25.762890544379236 26.412117184509338 25.406548102804067 22.41844416830736 64-65 28.850536322178687 21.815273941807437 27.298263643565207 22.03592609244867 66-67 28.816661185788085 25.06410058782423 24.097796001699074 22.021442224688617 68-69 23.16240908341523 28.71895668334586 25.194744020434683 22.923890212804224 70-71 23.84563563221886 28.412128261803392 23.47453449613117 24.26770160984658 72-73 22.753225154492267 28.610443129033587 23.70437168578123 24.93196003069292 74-75 21.175731502651427 28.83084192553912 25.73961773052713 24.25380884128233 76-77 22.12160087146492 28.61018644519217 23.900884567723082 25.367328115619824 78-79 22.799716360403842 29.30355859786808 21.765142646223833 26.131582395504243 80-81 21.906897995781407 24.320044669504757 25.476647423496196 28.296409911217644 82-83 24.117814809835085 21.339868133599726 27.52717528605452 27.01514177051067 84-85 25.155732386554657 22.45090334420444 29.848068575784765 22.545295693456136 86-87 26.317662307966028 24.34697637891706 25.030049299263617 24.305312013853296 88-89 22.968341798932734 26.401577770735006 26.49023101236006 24.139849417972194 90-91 20.714861023271904 27.99890896213953 26.136950943118304 25.14927907147026 92-93 23.420589212727105 24.321341574134664 26.546948927816473 25.71112028532176 94-95 22.233889883637918 23.859729965966306 27.698209751528776 26.208170398866997 96-97 22.04726533695437 22.970381656376514 27.765730489602365 27.216622517066753 98-99 21.479937721810384 24.785554748626318 28.724658805957876 25.009848723605423 100-101 20.500783812820103 28.345763150221014 26.55628915049763 24.597163886461253 102-103 21.303942718016746 27.541005928764058 26.098294334917366 25.05675701830184 104-105 21.439686150114927 25.232595581711614 25.82684475015672 27.50087351801674 106-107 20.662197436301476 26.760187652866176 24.086185693438658 28.491429217393687 108-109 22.380463425015222 26.868228658633193 25.482809324679263 25.268498591672326 110-111 23.17364864344089 27.97480853337809 24.038855970341622 24.81268685283939 112-113 21.012342771503505 28.34838088101483 25.380891719745225 25.258384627736447 114-115 22.032592600205376 25.922319370826468 26.708443706765944 25.33664432220221 116-117 24.794403000093347 25.172754575887407 24.99890810715451 25.033934316864737 118-119 22.86540853875152 26.091020079508976 25.498752342437967 25.544819039301537 120-121 20.89937234570058 26.392696292908763 27.16769391640354 25.540237444987113 122-123 21.770457349306994 27.592461827607277 27.624192756184428 23.0128880669013 124-125 22.030309066276075 29.170909409358703 24.15049273052903 24.64828879383619 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 127.0 1 166.5 2 227.5 3 252.5 4 230.0 5 192.0 6 183.0 7 173.5 8 159.0 9 163.0 10 197.0 11 215.0 12 207.5 13 256.5 14 318.5 15 377.0 16 444.5 17 533.5 18 669.5 19 784.0 20 939.0 21 1152.5 22 1330.5 23 1651.0 24 2028.0 25 2386.5 26 2896.0 27 3422.5 28 3912.0 29 4554.5 30 5389.0 31 6135.0 32 7168.0 33 8390.0 34 9533.5 35 10641.0 36 11788.5 37 12964.0 38 13676.5 39 14223.5 40 14530.0 41 14946.0 42 15673.5 43 17131.0 44 18905.0 45 22568.5 46 35576.5 47 61602.0 48 86529.0 49 104551.5 50 106963.0 51 89049.0 52 68936.0 53 54424.0 54 45959.0 55 41916.0 56 38256.0 57 33527.0 58 29582.5 59 25777.0 60 22378.5 61 18797.0 62 14774.5 63 11293.5 64 8713.0 65 7233.0 66 6028.5 67 4950.0 68 3857.5 69 2877.0 70 2281.0 71 1781.0 72 1412.5 73 1209.0 74 959.0 75 729.5 76 515.5 77 370.0 78 269.0 79 183.5 80 182.5 81 177.0 82 155.0 83 85.0 84 23.5 85 19.5 86 12.0 87 5.5 88 5.5 89 3.5 90 3.0 91 3.0 92 2.0 93 2.0 94 2.0 95 1.5 96 1.5 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16219961171434027 2 0.018669226691513295 3 0.008735142763919065 4 0.01567187378232538 5 0.0033399075273808187 6 0.0 7 3.425546181929045E-4 8 4.2819327274113057E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.563865454822611E-4 28-29 0.007664659582066238 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.2819327274113057E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0018840504000609745 66-67 8.563865454822612E-5 68-69 0.006380079763842846 70-71 0.01931151660062499 72-73 8.563865454822612E-5 74-75 0.01006254190941657 76-77 6.85109236385809E-4 78-79 0.00154149578186807 80-81 8.563865454822611E-4 82-83 0.0 84-85 0.019654071218817896 86-87 0.002783256272817349 88-89 0.01943997458244733 90-91 1.2845798182233918E-4 92-93 0.014815487236843118 94-95 0.015200861182310137 96-97 0.006508537745665185 98-99 0.002826075600091462 100-101 6.42289909111696E-4 102-103 0.0 104-105 4.2819327274113057E-4 106-107 7.70747890934035E-4 108-109 0.0 110-111 0.0 112-113 8.135672182081481E-4 114-115 0.005994705818375828 116-117 0.0 118-119 0.07797399496615988 120-121 0.001370218472771618 122-123 0.005694970527457037 124-125 0.253747333426394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1167697.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.78361848370528 #Duplication Level Percentage of deduplicated Percentage of total 1 82.57358816941495 40.28238422087338 2 9.770617787747064 9.532921810151146 3 2.705738213412247 3.9598710215965625 4 1.259627776154226 2.457968034535435 5 0.7394871247617453 1.8037428883994573 6 0.48690191884544715 1.4251702468764191 7 0.34031894583128613 1.1621392731347158 8 0.269051583177737 1.0500247828943694 9 0.2177338837088045 0.9559662042443197 >10 1.3946625731250157 13.135584056622438 >50 0.13918023953370467 4.700372472630564 >100 0.09024929296813883 8.501820269203007 >500 0.007212906083610541 2.4013095136574303 >1k 0.004574038004240831 4.270606244071028 >5k 8.796226931232368E-4 3.447140740727818 >10k+ 1.7592453862464734E-4 0.9129782203819778 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 10638 0.9110240070840295 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9766 0.8363471003179763 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 8913 0.7632973279883395 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8707 0.7456557651514049 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6534 0.5595629688181095 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 6246 0.5348990363082203 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4071 0.34863496266582855 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3446 0.29511080357318725 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3220 0.2757564676452881 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 2954 0.25297658553546 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2931 0.2510068964808508 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2662 0.22797009840737795 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 2531 0.21675143466156033 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1989 0.17033528389642175 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1988 0.17024964524187353 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1970 0.16870814946000548 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1937 0.165882073859914 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1851 0.15851714956876656 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1802 0.1543208554959035 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1453 0.12443296505857257 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1451 0.1242616877494761 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1446 0.12383349447673496 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1429 0.12237763734941513 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1368 0.11715367942197333 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1348 0.11544090633100881 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1251 0.10713395683983089 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 8.563865454822612E-5 0.0 0.0 4.281932727411306E-5 0.0 14-15 8.563865454822612E-5 0.0 0.0 1.7127730909645224E-4 0.0 16-17 8.563865454822612E-5 0.0 0.0 1.7127730909645224E-4 0.0 18-19 1.2845798182233918E-4 0.0 0.0 3.425546181929045E-4 8.563865454822612E-5 20-21 1.7127730909645224E-4 0.0 0.0 0.0010276638545787136 8.563865454822612E-5 22-23 2.5691596364467835E-4 0.0 0.0 0.0025263403091726707 8.563865454822612E-5 24-25 2.5691596364467835E-4 0.0 0.0 0.0036396428182996097 8.563865454822612E-5 26-27 2.997352909187914E-4 0.0 0.0 0.0058662478365534895 8.563865454822612E-5 28-29 3.8537394546701755E-4 0.0 0.0 0.014001920018634971 8.563865454822612E-5 30-31 4.281932727411306E-4 0.0 0.0 0.04234831467409782 8.563865454822612E-5 32-33 4.281932727411306E-4 0.0 0.0 0.09959775523958697 8.563865454822612E-5 34-35 4.710126000152437E-4 0.0 0.0 0.1649828679871576 8.563865454822612E-5 36-37 5.138319272893567E-4 0.0 0.0 0.24882311078987102 8.563865454822612E-5 38-39 5.994705818375828E-4 0.0 0.0 0.3987335755765409 8.563865454822612E-5 40-41 5.994705818375828E-4 0.0 0.0 0.6245627076202132 8.563865454822612E-5 42-43 5.994705818375828E-4 0.0 0.0 1.0689416860709584 8.563865454822612E-5 44-45 5.994705818375828E-4 0.0 0.0 1.6669564107812214 8.563865454822612E-5 46-47 5.994705818375828E-4 0.0 0.0 2.2525107112547174 8.563865454822612E-5 48-49 5.994705818375828E-4 0.0 0.0 2.5138370656086297 8.563865454822612E-5 50-51 5.994705818375828E-4 0.0 0.0 2.8398206041464524 8.563865454822612E-5 52-53 5.994705818375828E-4 0.0 0.0 3.1055573492095983 1.7127730909645224E-4 54-55 5.994705818375828E-4 0.0 0.0 3.3778882706729574 2.5691596364467835E-4 56-57 5.994705818375828E-4 0.0 0.0 3.76904282532198 2.5691596364467835E-4 58-59 5.994705818375828E-4 0.0 0.0 4.222456681827563 2.5691596364467835E-4 60-61 5.994705818375828E-4 0.0 0.0 4.871255128684925 2.5691596364467835E-4 62-63 6.85109236385809E-4 0.0 0.0 5.657417977437641 2.5691596364467835E-4 64-65 6.85109236385809E-4 0.0 0.0 6.364065335442328 2.5691596364467835E-4 66-67 7.707478909340351E-4 0.0 0.0 6.950861396406774 2.5691596364467835E-4 68-69 7.707478909340351E-4 0.0 0.0 7.442170357549946 2.5691596364467835E-4 70-71 7.707478909340351E-4 0.0 0.0 8.019117973241347 2.5691596364467835E-4 72-73 7.707478909340351E-4 0.0 0.0 8.600176244351061 2.5691596364467835E-4 74-75 7.707478909340351E-4 0.0 0.0 9.215960989880081 2.5691596364467835E-4 76-77 7.707478909340351E-4 0.0 0.0 9.941919864485392 2.5691596364467835E-4 78-79 7.707478909340351E-4 0.0 0.0 10.816547443386426 2.5691596364467835E-4 80-81 7.707478909340351E-4 0.0 0.0 12.548546412297025 2.5691596364467835E-4 82-83 7.707478909340351E-4 0.0 0.0 13.763202269081791 2.5691596364467835E-4 84-85 7.707478909340351E-4 0.0 0.0 14.384639165810995 2.5691596364467835E-4 86-87 7.707478909340351E-4 0.0 0.0 14.956491281556774 2.5691596364467835E-4 88-89 7.707478909340351E-4 0.0 0.0 15.61929164843277 2.5691596364467835E-4 90-91 7.707478909340351E-4 0.0 0.0 16.39856058549435 2.5691596364467835E-4 92-93 7.707478909340351E-4 0.0 0.0 17.427337742582193 2.5691596364467835E-4 94-95 7.707478909340351E-4 0.0 0.0 18.802437618663063 2.5691596364467835E-4 96-97 7.707478909340351E-4 0.0 0.0 20.35339647185871 2.5691596364467835E-4 98-99 9.420252000304874E-4 0.0 0.0 21.852158565107217 2.5691596364467835E-4 100-101 9.848445273046005E-4 0.0 0.0 23.09182090902006 2.5691596364467835E-4 102-103 0.0010276638545787134 0.0 0.0 24.15442533465445 2.5691596364467835E-4 104-105 0.0012417604909492788 8.563865454822612E-5 0.0 25.282714608327332 2.5691596364467835E-4 106-107 0.001370218472771618 8.563865454822612E-5 0.0 26.37225239081714 2.5691596364467835E-4 108-109 0.001370218472771618 8.563865454822612E-5 0.0 27.313421204302145 2.5691596364467835E-4 110-111 0.001370218472771618 8.563865454822612E-5 0.0 28.294925824079364 2.5691596364467835E-4 112-113 0.001370218472771618 8.563865454822612E-5 0.0 30.862415506762456 2.5691596364467835E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 2105 0.0 92.82481 1 AGTAGTA 1800 0.0 89.573746 2 AGTCCGT 255 0.0 88.659935 2 GTCGCAA 30 4.2387364E-5 79.42935 1 GAGTCCG 750 0.0 78.63507 1 GAGTTAG 1165 0.0 77.72487 1 GAGTAAG 1265 0.0 77.70264 1 GAGTCAG 5035 0.0 77.141914 1 GAGTACG 545 0.0 72.142265 1 GAGTCGT 620 0.0 72.06293 1 GAGTCGG 805 0.0 69.56236 1 GTGGGGT 5890 0.0 68.883606 7 TGGGGTA 6195 0.0 68.75431 8 GAGTTAC 655 0.0 68.212234 1 TAAGCGG 170 0.0 66.492096 4 TCGTAGG 855 0.0 65.41024 2 GAGTCGC 410 0.0 65.38393 1 GAGTAAC 1100 0.0 64.987656 1 AGTAAGG 720 0.0 64.45344 2 AGTTAGT 860 0.0 64.338135 2 >>END_MODULE