##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935972_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1167697 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.547421120376264 33.0 31.0 34.0 31.0 34.0 2 32.603380842804256 34.0 31.0 34.0 31.0 34.0 3 32.810430274292045 34.0 31.0 34.0 31.0 34.0 4 36.06639907441742 37.0 35.0 37.0 35.0 37.0 5 36.10364075612081 37.0 35.0 37.0 35.0 37.0 6 36.210349088847536 37.0 37.0 37.0 35.0 37.0 7 36.24075766230452 37.0 37.0 37.0 35.0 37.0 8 36.23880510098082 37.0 37.0 37.0 35.0 37.0 9 38.0682325980113 39.0 39.0 39.0 35.0 39.0 10-11 37.8556633270446 39.0 38.0 39.0 35.0 39.0 12-13 37.74892159524261 39.0 38.0 39.0 35.0 39.0 14-15 39.2807483448189 41.0 39.0 41.0 36.0 41.0 16-17 39.32565639887745 41.0 39.0 41.0 35.5 41.0 18-19 39.32767233280551 41.0 39.0 41.0 35.0 41.0 20-21 39.30792277448687 41.0 39.0 41.0 35.0 41.0 22-23 39.1192565365844 41.0 38.5 41.0 35.0 41.0 24-25 38.85958472103636 41.0 38.0 41.0 35.0 41.0 26-27 38.73744730011296 40.0 38.0 41.0 35.0 41.0 28-29 38.62106479677519 40.0 38.0 41.0 35.0 41.0 30-31 38.48241795602797 40.0 37.5 41.0 35.0 41.0 32-33 38.28344296508426 40.0 37.0 41.0 34.5 41.0 34-35 37.8466074675194 40.0 37.0 41.0 33.5 41.0 36-37 37.456731926176055 40.0 37.0 41.0 33.0 41.0 38-39 36.86978085924687 40.0 37.0 41.0 31.5 41.0 40-41 36.52468791133316 40.0 36.5 41.0 30.5 41.0 42-43 36.33722832207327 40.0 36.0 41.0 30.0 41.0 44-45 36.162386732174525 40.0 35.0 41.0 30.0 41.0 46-47 35.96825931727152 40.0 35.0 41.0 29.5 41.0 48-49 35.736479583316566 40.0 35.0 41.0 28.0 41.0 50-51 35.45979821820215 39.5 35.0 41.0 27.0 41.0 52-53 35.199007105439165 39.0 35.0 41.0 26.0 41.0 54-55 34.96010437639217 39.0 35.0 41.0 26.0 41.0 56-57 34.7172425723454 38.5 35.0 41.0 26.0 41.0 58-59 34.42013339076833 37.0 35.0 40.5 25.5 41.0 60-61 34.12674520873138 37.0 35.0 40.0 25.5 41.0 62-63 33.74186753926746 36.5 35.0 39.0 24.0 41.0 64-65 33.34177445004997 36.0 34.0 39.0 23.0 41.0 66-67 32.932462787863635 35.0 34.0 38.5 23.0 41.0 68-69 32.591754110869516 35.0 34.0 37.0 22.0 40.0 70-71 32.36528825542928 35.0 34.0 37.0 22.0 39.0 72-73 32.07181914486378 35.0 34.0 36.0 20.0 39.0 74-75 31.770484123878028 35.0 33.5 36.0 20.0 38.5 76-77 31.58162605538937 35.0 33.0 35.0 20.0 37.0 78-79 31.550788432273098 35.0 34.0 35.0 20.0 37.0 80-81 31.447510355854302 35.0 33.5 35.0 20.0 36.5 82-83 31.327672760998787 35.0 33.0 35.0 20.0 36.0 84-85 31.154133306842443 35.0 33.0 35.0 19.0 36.0 86-87 31.05063428269491 35.0 33.0 35.0 18.0 35.5 88-89 30.96384164727665 35.0 33.0 35.0 17.0 35.5 90-91 30.92236641868567 35.0 33.0 35.0 16.0 35.0 92-93 30.891316839899392 35.0 33.0 35.0 15.5 36.0 94-95 30.796659150447418 35.0 33.0 35.0 15.0 35.0 96-97 30.75922777912421 35.0 33.0 35.0 14.0 35.0 98-99 30.680557113703298 35.0 33.0 35.0 10.0 35.0 100-101 30.639229611791414 35.0 33.0 35.0 9.0 35.0 102-103 29.97820967254348 34.0 31.5 35.0 7.0 35.0 104-105 30.527758057098715 35.0 33.0 35.0 7.0 35.0 106-107 30.613603100804404 35.0 33.0 35.0 4.5 35.0 108-109 30.551060763194563 35.0 33.0 35.0 2.0 35.0 110-111 30.470372879265767 35.0 33.0 35.0 2.0 35.0 112-113 30.412332137532253 35.0 33.0 35.0 2.0 35.0 114-115 30.282651235722966 35.0 33.0 35.0 2.0 35.0 116-117 30.139689063173066 35.0 33.0 35.0 2.0 35.0 118-119 29.948529027650153 35.0 33.0 35.0 2.0 35.0 120-121 29.680942487648764 35.0 33.0 35.0 2.0 35.0 122-123 29.381598137187986 35.0 32.5 35.0 2.0 35.0 124-125 27.194207058851738 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01069237506447962 1101 2 0.055055708202360165 1101 3 0.04622685619614231 1101 4 0.1690175631202706 1101 5 0.17298246442518916 1101 6 0.1686636569136013 1101 7 0.20195941508141146 1101 8 0.10027407946547129 1101 9 0.1698195131821194 1101 10-11 0.09838809584920227 1101 12-13 0.1392022359017986 1101 14-15 0.17073976649267308 1101 16-17 0.10021307863746642 1101 18-19 0.11976146673540455 1101 20-21 0.13825409625662388 1101 22-23 0.16797442018480524 1101 24-25 0.24153746428658707 1101 26-27 0.1740960550775128 1101 28-29 0.22897934383600926 1101 30-31 0.3075809130041165 1101 32-33 0.37253408322767 1101 34-35 0.4568117756650665 1101 36-37 0.2142352192096837 1101 38-39 0.34052586473760726 1101 40-41 0.5098509448770372 1101 42-43 0.47677157759841293 1101 44-45 0.546443041210857 1101 46-47 0.5730706338555009 1101 48-49 0.4741492805306251 1101 50-51 0.5242741429049005 1101 52-53 0.6297691823376539 1101 54-55 0.5511312836143532 1101 56-57 0.5327417817229474 1101 58-59 0.4694371193995295 1101 60-61 0.38454787502249843 1101 62-63 0.4105877853165154 1101 64-65 0.3580020961068442 1101 66-67 0.31049744340434415 1101 68-69 0.49075670315835396 1101 70-71 0.5403215693551218 1101 72-73 0.541933748325178 1101 74-75 0.5540855872099293 1101 76-77 0.5898224583167746 1101 78-79 0.5688175711633754 1101 80-81 0.5895192946524368 1101 82-83 0.6420216770224627 1101 84-85 0.6241231328604613 1101 86-87 0.4848612553471554 1101 88-89 0.31093861891531915 1101 90-91 0.3926511164884978 1101 92-93 0.40588326544018116 1101 94-95 0.32027317160972757 1101 96-97 0.4459849332879422 1101 98-99 0.4291792304809334 1101 100-101 0.32093983996135833 1101 102-103 0.3787631984921056 1101 104-105 0.4267606449312886 1101 106-107 0.35185652324887684 1101 108-109 0.3767374397843568 1101 110-111 0.2610256607781025 1101 112-113 0.4107887475468388 1101 114-115 0.43698244138295195 1101 116-117 0.3716757194725204 1101 118-119 0.15483482257054249 1101 120-121 0.2845797749042127 1101 122-123 0.21536457205332127 1101 124-125 0.24016912598035844 1102 1 0.117440962425988 1102 2 0.11557050903472543 1102 3 0.167506085681012 1102 4 0.23542884448824708 1102 5 0.20803605010058135 1102 6 0.1643948978147307 1102 7 0.1941563866476912 1102 8 0.2033966890837462 1102 9 0.19627307089261592 1102 10-11 0.2603745493246663 1102 12-13 0.2039084811210401 1102 14-15 0.2793677593533985 1102 16-17 0.22478126774596774 1102 18-19 0.160220502275493 1102 20-21 0.14236287970141603 1102 22-23 0.18064005050533893 1102 24-25 0.2895529848037697 1102 26-27 0.30218679293950856 1102 28-29 0.2607437642635446 1102 30-31 0.24952517238674687 1102 32-33 0.22874948695028507 1102 34-35 0.1461446072752679 1102 36-37 0.2332988475726694 1102 38-39 0.35095306964627326 1102 40-41 0.2431890075677572 1102 42-43 0.16961462050807796 1102 44-45 0.10792859405216859 1102 46-47 0.09744676992939816 1102 48-49 0.19739489456570425 1102 50-51 0.04520175494954515 1102 52-53 0.07560426347697558 1102 54-55 0.09743950611621699 1102 56-57 -0.013885158341700787 1102 58-59 0.0074547784087499736 1102 60-61 -0.13019517693464167 1102 62-63 -0.05064073723223572 1102 64-65 -0.099735749444676 1102 66-67 -0.15593131602352628 1102 68-69 -0.03498847906584501 1102 70-71 -0.0050228923617581245 1102 72-73 -0.12187823710017298 1102 74-75 -0.14418922546639124 1102 76-77 -0.10314437038178426 1102 78-79 -0.0336441621666701 1102 80-81 -0.005758544812611177 1102 82-83 -0.019059412950475974 1102 84-85 -0.019916437467138337 1102 86-87 0.03702257167125467 1102 88-89 -0.009839504311170089 1102 90-91 -0.05945098005619087 1102 92-93 -0.01758606776155247 1102 94-95 0.07501013708953153 1102 96-97 0.06765669943561292 1102 98-99 -0.06789823228051617 1102 100-101 -0.07551684323749441 1102 102-103 -0.08050202386994343 1102 104-105 -0.019151956544266824 1102 106-107 0.07807505245985524 1102 108-109 -0.008357270737846534 1102 110-111 0.006748676709868562 1102 112-113 -0.08971359245694188 1102 114-115 -0.028062466949762666 1102 116-117 -0.03903709749723561 1102 118-119 0.021626303147279202 1102 120-121 0.058662896444584334 1102 122-123 0.07468305109198425 1102 124-125 0.16336518457504212 1103 1 0.047854664582544615 1103 2 0.11447564734343274 1103 3 0.13966074822039332 1103 4 0.20038418929269142 1103 5 0.1647907900265082 1103 6 0.22070545476096726 1103 7 0.20844052161614712 1103 8 0.24814861782495257 1103 9 0.24784370190882754 1103 10-11 0.3053410677987287 1103 12-13 0.25530459757989377 1103 14-15 0.2591785571455105 1103 16-17 0.26591359873927445 1103 18-19 0.30486740641882903 1103 20-21 0.2438865260291081 1103 22-23 0.3409541767085784 1103 24-25 0.3062686761104203 1103 26-27 0.24102141473251493 1103 28-29 0.3979615600104438 1103 30-31 0.3352866402042096 1103 32-33 0.39486266872180664 1103 34-35 0.4227724885421935 1103 36-37 0.3761072290093779 1103 38-39 0.4385874103207996 1103 40-41 0.4114172899812658 1103 42-43 0.4847488229923371 1103 44-45 0.4014164767404864 1103 46-47 0.24740708958321278 1103 48-49 0.28611916156508244 1103 50-51 0.3793355389147308 1103 52-53 0.35751811961696944 1103 54-55 0.38486814071748654 1103 56-57 0.3686148219168288 1103 58-59 0.4321266752477513 1103 60-61 0.4922222445140747 1103 62-63 0.5622024681500761 1103 64-65 0.44031040610228445 1103 66-67 0.4170084646093031 1103 68-69 0.4447932987716001 1103 70-71 0.47562987894520603 1103 72-73 0.4168760369271638 1103 74-75 0.3142601218807535 1103 76-77 0.2715502291428429 1103 78-79 0.33452469843489396 1103 80-81 0.48027298625650516 1103 82-83 0.33966907872257224 1103 84-85 0.4071552319501741 1103 86-87 0.39598144624977394 1103 88-89 0.33220843586429183 1103 90-91 0.2889497638022398 1103 92-93 0.2880672957893857 1103 94-95 0.28717976365996734 1103 96-97 0.34343253904015114 1103 98-99 0.2505511545495587 1103 100-101 0.21207840933481847 1103 102-103 0.37355346544202916 1103 104-105 0.26771917906380693 1103 106-107 0.2752656444669306 1103 108-109 0.2692898624064952 1103 110-111 0.35151166067189976 1103 112-113 0.2814992047508227 1103 114-115 0.3640119267898214 1103 116-117 0.4414415540816883 1103 118-119 0.2816618772685828 1103 120-121 0.25476198280954776 1103 122-123 0.15977192717085487 1103 124-125 0.13486482528036348 1104 1 0.01640024332763801 1104 2 0.016809350213279117 1104 3 0.05909013723900358 1104 4 0.12864104325788617 1104 5 0.14424922020804587 1104 6 0.21643856176267917 1104 7 0.11971490589714051 1104 8 0.1286923303979819 1104 9 0.11157780886267687 1104 10-11 0.16971211234599082 1104 12-13 0.2026256984694399 1104 14-15 0.2711322022217715 1104 16-17 0.23256546115011645 1104 18-19 0.2544024970641843 1104 20-21 0.22518947277253432 1104 22-23 0.32411512721019164 1104 24-25 0.26672307684959407 1104 26-27 0.21764031519548155 1104 28-29 0.26569604536562963 1104 30-31 0.24715503100313185 1104 32-33 0.31161038520651374 1104 34-35 0.4258787228182115 1104 36-37 0.4352835374383801 1104 38-39 0.2912466295223126 1104 40-41 0.34354477931878336 1104 42-43 0.21372646312051558 1104 44-45 0.24347608938820287 1104 46-47 0.2704054186905509 1104 48-49 0.22671888969360054 1104 50-51 0.22413837130193315 1104 52-53 0.25165654612086996 1104 54-55 0.37096284135111546 1104 56-57 0.2977878554266269 1104 58-59 0.3244844119214463 1104 60-61 0.17795442450849208 1104 62-63 0.19595765312222824 1104 64-65 0.30202846215279067 1104 66-67 0.21624717886538747 1104 68-69 0.2433595528187027 1104 70-71 0.21507339342741716 1104 72-73 0.0933320652479992 1104 74-75 0.1121907680580243 1104 76-77 0.127708710917652 1104 78-79 0.1548277538357361 1104 80-81 0.17928169632585522 1104 82-83 0.15008981845919322 1104 84-85 0.1835984949246665 1104 86-87 0.08718638856636218 1104 88-89 0.19875490818362707 1104 90-91 0.1679534665125182 1104 92-93 0.1364854566221112 1104 94-95 0.11147654884426217 1104 96-97 0.13188795492829897 1104 98-99 0.1054828713442717 1104 100-101 0.17143654796495866 1104 102-103 0.17244398208142897 1104 104-105 0.16757617679111902 1104 106-107 0.14027343664709235 1104 108-109 0.2069220274320287 1104 110-111 0.19144162603070214 1104 112-113 0.2165765908638022 1104 114-115 0.21786523776354016 1104 116-117 0.23582259815024642 1104 118-119 0.2120990979791415 1104 120-121 0.2036743828998162 1104 122-123 0.36595411601743777 1104 124-125 0.21134387681512834 1105 1 0.0016822985555009495 1105 2 -0.0014683105364952098 1105 3 0.16247070600961422 1105 4 0.15880281289548037 1105 5 0.1745445517828088 1105 6 0.092752949141655 1105 7 0.08566677423991109 1105 8 0.1505195448040908 1105 9 0.11940715154921833 1105 10-11 0.13615757915221138 1105 12-13 0.14358878165965194 1105 14-15 0.1336947912328199 1105 16-17 0.11949657077398257 1105 18-19 0.1399235846067839 1105 20-21 0.14692979933146688 1105 22-23 0.06627997748290682 1105 24-25 0.1043411593937762 1105 26-27 0.1180593657733553 1105 28-29 0.07479756550371519 1105 30-31 0.10260109157928099 1105 32-33 0.18997102856855008 1105 34-35 0.26248601733244215 1105 36-37 0.15045798820120382 1105 38-39 -0.05810457885028342 1105 40-41 -0.06243741629428001 1105 42-43 -0.11007412890729285 1105 44-45 -0.12157118718227622 1105 46-47 -0.15031540927731868 1105 48-49 -0.11437506967743616 1105 50-51 -0.15772065116985345 1105 52-53 -0.09659930919090698 1105 54-55 -0.1110433170156 1105 56-57 -0.11313758362194193 1105 58-59 -0.12151845675668227 1105 60-61 -0.1092581814155622 1105 62-63 -0.09266824134352447 1105 64-65 -0.07745221419325077 1105 66-67 0.007420549518627695 1105 68-69 -0.007501340971970194 1105 70-71 -0.055764809351536826 1105 72-73 0.007988165036181272 1105 74-75 -0.09419073419438106 1105 76-77 -0.07850910537371192 1105 78-79 0.05082800934552978 1105 80-81 0.0829505120815206 1105 82-83 0.16351025256452445 1105 84-85 0.11037316287081822 1105 86-87 0.19083625885158995 1105 88-89 0.20949974826498163 1105 90-91 0.1734781399027696 1105 92-93 0.09363094767273594 1105 94-95 0.14190072413559207 1105 96-97 0.17125388414918064 1105 98-99 0.16436553863422532 1105 100-101 0.17816218996960842 1105 102-103 0.2025832494361346 1105 104-105 0.2775582620704178 1105 106-107 0.3031866983312206 1105 108-109 0.2741399758335703 1105 110-111 0.23814983239550713 1105 112-113 0.24239251638269366 1105 114-115 0.24691641085835414 1105 116-117 0.24059997798442723 1105 118-119 0.3008673375311872 1105 120-121 0.3602380084676362 1105 122-123 0.3762053576353388 1105 124-125 0.5172094234972064 1106 1 0.022628092115354548 1106 2 0.07836073246319586 1106 3 0.11654503920818371 1106 4 0.14718794513858313 1106 5 0.17614910653981042 1106 6 0.15605989599794157 1106 7 0.16264874499344728 1106 8 0.10868800629582154 1106 9 0.1716888940482022 1106 10-11 0.17946292703965838 1106 12-13 0.14526944454046742 1106 14-15 0.2144652358648571 1106 16-17 0.17138887009815562 1106 18-19 0.20051141163605735 1106 20-21 0.1784492107433593 1106 22-23 0.12074371894430413 1106 24-25 0.04323977708870075 1106 26-27 0.04952871340105247 1106 28-29 0.1606173302947056 1106 30-31 0.1267928267956293 1106 32-33 0.10280994537539101 1106 34-35 0.007678462658539331 1106 36-37 -0.1397412405232643 1106 38-39 -0.4087018879742459 1106 40-41 -0.5539358246569606 1106 42-43 -0.5864579177372704 1106 44-45 -0.5546868093871282 1106 46-47 -0.5713575948233327 1106 48-49 -0.5981382044383992 1106 50-51 -0.5443453815620671 1106 52-53 -0.5401771800207626 1106 54-55 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24-25 -0.2983345587662143 2216 26-27 -0.2829963538837035 2216 28-29 -0.5900236790190334 2216 30-31 -0.5119207396783167 2216 32-33 -0.4231110279399459 2216 34-35 -0.4545295809470815 2216 36-37 -0.30512376601505053 2216 38-39 -0.3811694766755025 2216 40-41 -0.2759924881822542 2216 42-43 -0.2548243203590417 2216 44-45 -0.26115506032317626 2216 46-47 -0.24952167622726762 2216 48-49 -0.32857128900917587 2216 50-51 -0.4449040360368173 2216 52-53 -0.4573850962735264 2216 54-55 -0.6325947383649151 2216 56-57 -0.4956377419655027 2216 58-59 -0.3674657254743252 2216 60-61 -0.34654233036214066 2216 62-63 -0.1881338577605831 2216 64-65 -0.3057942708169037 2216 66-67 -0.5537467015796977 2216 68-69 -0.44700920920710274 2216 70-71 -0.44377877813195 2216 72-73 -0.37425547675039184 2216 74-75 -0.24322915697742786 2216 76-77 -0.43348742670172413 2216 78-79 -0.4031023655544246 2216 80-81 -0.423502995372143 2216 82-83 -0.38197753195004935 2216 84-85 -0.41019192208769795 2216 86-87 -0.5318195368103709 2216 88-89 -0.4966721955604818 2216 90-91 -0.378400102541363 2216 92-93 -0.5088369085858631 2216 94-95 -0.5502497540619409 2216 96-97 -0.5663141316045639 2216 98-99 -0.7354378274618298 2216 100-101 -0.5772718728956612 2216 102-103 -0.6331177518709197 2216 104-105 -0.6100221332702525 2216 106-107 -0.6542018997813237 2216 108-109 -0.5346123921223018 2216 110-111 -0.5326447067671722 2216 112-113 -0.3334960788508745 2216 114-115 -0.5930031506163687 2216 116-117 -0.5923438302068291 2216 118-119 -0.3975368473449876 2216 120-121 -0.5417162388822696 2216 122-123 -0.4821903054786745 2216 124-125 -0.9616332866534805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 19.0 4 79.0 5 162.0 6 474.0 7 956.0 8 1335.0 9 2479.0 10 6676.0 11 21108.0 12 29363.0 13 9144.0 14 6875.0 15 8588.0 16 5377.0 17 3334.0 18 2751.0 19 2553.0 20 2551.0 21 2706.0 22 2769.0 23 3120.0 24 3815.0 25 4792.0 26 6317.0 27 8020.0 28 10480.0 29 13108.0 30 17184.0 31 22348.0 32 29805.0 33 40694.0 34 71883.0 35 110464.0 36 219273.0 37 406619.0 38 89977.0 39 491.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 67.09170362640229 10.891221662295578 7.514984140497343 14.502090570804786 2 11.603613293962086 30.12953802728126 44.14509697795987 14.12175170079679 3 36.081363572913176 20.339779925785543 20.75512740034444 22.82372910095684 4 14.78767143843222 16.026358324008456 46.040120558892504 23.14584967866682 5 16.68030164279332 32.884735480035395 29.69175245279657 20.743210424374713 6 31.21481240418954 37.237918247450054 16.147627326213744 15.399642022146667 7 41.9245746113932 23.625306907528238 18.831940135154927 15.618178345923642 8 40.76948043884672 23.933606063901852 18.129446251895825 17.167467245355603 9 48.574844330335694 9.964143095340658 17.677017239917546 23.7839953344061 10-11 33.961978150153676 23.874729488899945 26.15494430490101 16.00834805604536 12-13 27.373839275085913 25.837781547781656 28.797710364932 17.990668812200425 14-15 23.872931077154433 25.50224929926171 28.160858510384113 22.463961113199744 16-17 21.986525614093384 30.111535783683607 27.948517466431788 19.95342113579122 18-19 22.511148011855816 24.39956598329875 30.308247773180884 22.78103823166455 20-21 22.85216113426685 24.692064807908213 29.070683576304468 23.38509048152046 22-23 23.29251509595383 22.564372435657535 29.381209337696333 24.761903130692296 24-25 20.233759271454836 21.28064900397963 38.01028862795742 20.475303096608112 26-27 17.795969331085033 21.741170868812716 35.87034992810635 24.5925098719959 28-29 20.813789878709972 22.50643788585566 29.260801389401532 27.41897084603283 30-31 20.938608217714012 25.552176634863326 28.69639983660145 24.812815310821215 32-33 27.187575201443526 22.29152768226689 26.98778022038251 23.53311689590707 34-35 25.45613288378749 18.546592138200236 32.003250843326654 23.994024134685624 36-37 23.079060749492378 24.337991790678576 32.43170103203143 20.151246427797624 38-39 22.24528280881085 27.935371932958635 27.832048896246203 21.987296361984317 40-41 20.967982276224053 26.54173128816808 28.94864849357325 23.541637942034622 42-43 21.951285307746787 22.463361642617905 26.138758599191398 29.446594450443907 44-45 22.729997593553808 23.256033029116285 25.792050506252906 28.221918871077 46-47 23.379481149647553 22.134209473861798 25.959559714549236 28.52674966194141 48-49 18.850652181173714 22.503997184201037 27.423081501451147 31.222269133174102 50-51 23.08492699732893 23.514490488542833 27.26777580142794 26.132806712700297 52-53 23.817565686989006 21.803001977396534 29.98804484382507 24.39138749178939 54-55 21.17248738328522 23.3059603647179 29.574281684375315 25.947270567621565 56-57 21.25816885716072 25.253083633853645 28.27968214356978 25.209065365415857 58-59 23.24421489478863 22.71775126595341 31.38601024067031 22.652023598587647 60-61 24.86261418844101 27.529744445690962 27.828837446700643 19.778803919167387 62-63 25.772653350997732 23.83293782548041 27.142143895205688 23.252264928316162 64-65 28.681413072055506 20.1053012896325 28.79749626829563 22.415789370016366 66-67 28.250136807750643 23.830668401134883 25.61417902075624 22.305015770358235 68-69 22.210770431027914 27.55543604205543 26.543486880586315 23.690306646330342 70-71 23.499075530724152 26.39246311329052 25.012267737264033 25.096193618721298 72-73 22.685636770497826 27.092302198258622 25.440803564623355 24.781257466620193 74-75 20.95804819229646 26.46105967558365 27.127414046623393 25.453478085496496 76-77 22.24125779204708 27.41053543855983 24.45964149946433 25.88856526992876 78-79 22.380463425015222 27.230137612753996 22.717066156717024 27.67233280551376 80-81 21.658315470537307 22.489181696964195 26.542287939422643 29.31021489307586 82-83 23.903418438173603 19.981895988428505 28.245897694350504 27.868787879047392 84-85 24.645434560506708 21.35125807465464 29.930367209986837 24.072940154851814 86-87 25.337352069929096 23.74828401545949 25.16076516425066 25.75359875036075 88-89 21.885985833653766 25.0680613207022 27.30061822544718 25.745334620196846 90-91 20.211150666654106 25.943759382785085 26.857823562105583 26.98726638845522 92-93 23.671894335602474 22.97903480098005 26.74559410532013 26.60347675809735 94-95 22.032599210240328 22.281293863048376 28.45515574673909 27.2309511799722 96-97 21.85472772474366 21.285744503925248 28.36031093682693 28.499216834504153 98-99 20.358748887767973 23.24896784011606 29.27172031785643 27.120562954259537 100-101 19.947469249300116 26.247776606431287 27.829308459300655 25.975445684967934 102-103 21.036493199862637 25.797702657453087 26.911518998507315 26.254285144176958 104-105 20.327319501548775 23.538469311816336 26.030254423878795 30.10395676275609 106-107 19.89532382772407 25.31847958780385 24.910496862840645 29.87569972163143 108-109 21.885086627781007 24.676136018162246 26.481313217384304 26.957464136672442 110-111 23.039505139877374 26.652684567350654 24.124602257375393 26.183208035396575 112-113 20.52888878802528 26.815684081482 25.157971676642887 27.497455453849835 114-115 21.619140395011506 23.906439008724508 26.30369854948349 28.170722046780494 116-117 23.72056495418729 23.945592563426313 25.021785498801208 27.312056983585187 118-119 21.37890901492246 25.039790796967807 25.698924224705706 27.882375963404026 120-121 19.91440383491373 25.02760786765351 27.08606932047607 27.97191897695669 122-123 20.06534380432828 26.372562453432906 27.853760052069504 25.70833369016931 124-125 20.515513461790512 26.81097468520338 24.74829548520955 27.92521636779656 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 40.0 2 239.0 3 594.5 4 854.0 5 838.0 6 756.5 7 819.5 8 972.0 9 1164.0 10 1361.0 11 1490.5 12 1632.5 13 1725.0 14 1780.0 15 1924.5 16 2077.0 17 2181.0 18 2310.0 19 2430.0 20 2483.0 21 2742.0 22 2933.0 23 3050.5 24 3256.5 25 3509.0 26 3840.5 27 4148.0 28 4391.5 29 4800.5 30 5523.0 31 6165.0 32 6982.0 33 7848.0 34 8917.0 35 9927.0 36 10845.0 37 12195.5 38 13100.5 39 13896.0 40 14434.0 41 15018.5 42 15946.0 43 17188.0 44 18896.0 45 22055.5 46 33999.5 47 59947.5 48 84407.0 49 100042.5 50 102605.5 51 89143.0 52 72191.5 53 56988.0 54 46655.0 55 41063.5 56 36332.5 57 31624.0 58 28336.0 59 24177.0 60 20248.0 61 17257.0 62 13499.5 63 10132.0 64 7936.0 65 6716.5 66 5481.0 67 4372.0 68 3478.0 69 2665.0 70 2045.5 71 1513.5 72 1238.5 73 1112.5 74 885.0 75 645.0 76 434.5 77 319.5 78 251.0 79 151.0 80 140.5 81 157.5 82 133.5 83 62.5 84 11.0 85 7.0 86 5.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21109928346137738 2 7.70747890934035E-4 3 0.0 4 0.12520371294950658 5 0.030487361019168498 6 0.002312243672802105 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 4.281932727411306E-5 108-109 0.0 110-111 0.0011561218364010526 112-113 2.997352909187914E-4 114-115 8.135672182081481E-4 116-117 0.005352415909264133 118-119 0.0023550630000762182 120-121 3.853739454670175E-4 122-123 0.002312243672802105 124-125 0.030487361019168498 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1167697.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.75031345305446 #Duplication Level Percentage of deduplicated Percentage of total 1 82.34533525540986 39.320155698426774 2 9.807949287108773 9.36665305582212 3 2.7241885715799463 3.9024257459451337 4 1.2654453743455545 2.4170165313087226 5 0.7382191540124208 1.7625098000570887 6 0.503812343610332 1.4434318397346801 7 0.3822839443072778 1.277792471811977 8 0.2669407042743606 1.0197201841983867 9 0.220661560353835 0.9482992806542143 >10 1.4988491740469316 13.919903399098422 >50 0.13806720995982358 4.5901910158548915 >100 0.09442478234328869 8.909705665282805 >500 0.007719135868142633 2.4078431647188108 >1k 0.005026414053674273 4.424722546088298 >5k 8.975739381561202E-4 3.3909529281990545 >10k+ 1.7951478763122405E-4 0.8986766727985765 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10484 0.8978356542836026 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 9760 0.835833268390687 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9072 0.7769138740615074 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 8666 0.7421445803149276 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6735 0.5767763383823029 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 5326 0.45611147412385233 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4565 0.39094045801265226 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3222 0.2759277449543846 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3184 0.27267347608155196 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 3056 0.26171172829937905 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 2938 0.25160636706268835 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2652 0.2271137118618957 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 2113 0.1809544770604018 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1943 0.16639590578720337 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1897 0.16245652767798496 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1737 0.1487543429502688 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1704 0.1459282673501773 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1691 0.1448149648410504 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1631 0.13967664556815682 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1622 0.13890589767722278 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1608 0.1377069565135476 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1559 0.13351066244068452 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1504 0.12880053644053208 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1499 0.12837234316779098 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1349 0.11552654498555705 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1237 0.10593501567615572 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.281932727411306E-5 0.0 14-15 0.0 0.0 0.0 8.563865454822612E-5 0.0 16-17 0.0 0.0 0.0 8.563865454822612E-5 0.0 18-19 8.563865454822612E-5 0.0 0.0 8.563865454822612E-5 0.0 20-21 1.7127730909645224E-4 0.0 0.0 6.85109236385809E-4 0.0 22-23 1.7127730909645224E-4 0.0 0.0 0.002183785690979766 4.281932727411306E-5 24-25 1.7127730909645224E-4 0.0 0.0 0.0032970882001067056 8.563865454822612E-5 26-27 1.7127730909645224E-4 0.0 0.0 0.005480873891086472 8.563865454822612E-5 28-29 3.8537394546701755E-4 0.0 0.0 0.013530907418619727 8.563865454822612E-5 30-31 5.994705818375828E-4 0.0 0.0 0.04230549534682371 8.563865454822612E-5 32-33 5.994705818375828E-4 0.0 0.0 0.09976903254868344 8.563865454822612E-5 34-35 5.994705818375828E-4 0.0 0.0 0.1655395192417211 8.563865454822612E-5 36-37 6.85109236385809E-4 0.0 0.0 0.24967949733535327 1.7127730909645224E-4 38-39 6.85109236385809E-4 0.0 0.0 0.3997612394311195 1.7127730909645224E-4 40-41 6.85109236385809E-4 0.0 0.0 0.6266608546566447 1.7127730909645224E-4 42-43 7.27928563659922E-4 0.0 0.0 1.072538509561984 1.7127730909645224E-4 44-45 7.707478909340351E-4 0.0 0.0 1.671281162835907 1.7127730909645224E-4 46-47 7.707478909340351E-4 0.0 0.0 2.257606211200337 1.7127730909645224E-4 48-49 7.707478909340351E-4 0.0 0.0 2.519574855463361 1.7127730909645224E-4 50-51 7.707478909340351E-4 0.0 0.0 2.8469286124739552 1.7127730909645224E-4 52-53 7.707478909340351E-4 0.0 0.0 3.1145922272644357 1.7127730909645224E-4 54-55 7.707478909340351E-4 0.0 0.0 3.3875654386369067 1.7127730909645224E-4 56-57 9.420252000304874E-4 0.0 0.0 3.779619199158686 1.7127730909645224E-4 58-59 9.420252000304874E-4 0.0 0.0 4.235216841355249 1.7127730909645224E-4 60-61 9.420252000304874E-4 0.0 0.0 4.886712905830879 1.7127730909645224E-4 62-63 9.420252000304874E-4 0.0 0.0 5.6751023596018495 1.7127730909645224E-4 64-65 9.420252000304874E-4 0.0 0.0 6.384361696570258 1.7127730909645224E-4 66-67 9.420252000304874E-4 0.0 0.0 6.973555639862052 1.7127730909645224E-4 68-69 0.0010276638545787134 0.0 0.0 7.466834290059836 1.7127730909645224E-4 70-71 0.0010276638545787134 0.0 0.0 8.046693620005875 1.7127730909645224E-4 72-73 0.0010276638545787134 0.0 0.0 8.629935676806568 1.7127730909645224E-4 74-75 0.0010276638545787134 0.0 0.0 9.248717775244778 1.7127730909645224E-4 76-77 0.0010276638545787134 0.0 0.0 9.978102196032019 1.7127730909645224E-4 78-79 0.0010276638545787134 0.0 0.0 10.857996552187767 1.7127730909645224E-4 80-81 0.0011133025091269397 0.0 0.0 12.594363092480325 1.7127730909645224E-4 82-83 0.0011133025091269397 0.0 0.0 13.815570306338032 1.7127730909645224E-4 84-85 0.0011989411636751657 0.0 0.0 14.44176014839466 1.7127730909645224E-4 86-87 0.001284579818223392 0.0 0.0 15.017722919558755 1.7127730909645224E-4 88-89 0.001284579818223392 0.0 0.0 15.686817727544046 1.7127730909645224E-4 90-91 0.0015414957818680702 0.0 0.0 16.474864626696824 1.7127730909645224E-4 92-93 0.0015414957818680702 0.0 0.0 17.512719481166776 1.7127730909645224E-4 94-95 0.0015414957818680702 0.0 0.0 18.89685423530248 1.7127730909645224E-4 96-97 0.0015414957818680702 0.0 0.0 20.461429634571296 1.7127730909645224E-4 98-99 0.0015843151091421833 0.0 0.0 21.972181139456552 1.7127730909645224E-4 100-101 0.0017127730909645223 0.0 0.0 23.221178096715157 1.7127730909645224E-4 102-103 0.0017984117455127485 0.0 0.0 24.292731761749838 1.7127730909645224E-4 104-105 0.0019268697273350879 0.0 0.0 25.429028249622974 1.7127730909645224E-4 106-107 0.001969689054609201 0.0 0.0 26.5284572967131 1.7127730909645224E-4 108-109 0.001969689054609201 0.0 0.0 27.476948215161983 1.7127730909645224E-4 110-111 0.001969689054609201 0.0 0.0 28.467016700394026 1.7127730909645224E-4 112-113 0.001969689054609201 0.0 0.0 31.048593941750298 1.7127730909645224E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTCCG 540 0.0 87.33791 1 GAGTAGT 1965 0.0 86.283005 1 AGTAGTA 1555 0.0 84.67863 2 GAGTCGT 690 0.0 79.599106 1 GAGTCAG 4115 0.0 77.90653 1 GAGTTAG 1165 0.0 76.86609 1 GAGTACG 640 0.0 73.69135 1 GAGTAAC 875 0.0 73.68603 1 AGTCGTA 405 0.0 73.55749 2 AGTTTCG 100 0.0 71.49788 2 GAGTAAT 720 0.0 70.47837 1 GAGTCGA 370 0.0 69.380295 1 TCGTAGG 1135 0.0 69.2931 2 GAGTCGG 700 0.0 69.08066 1 GAGTAAG 1065 0.0 68.38796 1 GATTGTG 6565 0.0 67.152565 9 AGTACGG 370 0.0 66.02281 2 AGTGTCG 145 0.0 65.74518 2 AGTCCGT 265 0.0 65.20247 2 TACGCGG 120 0.0 64.5467 4 >>END_MODULE