FastQCFastQC Report
Thu 26 May 2016
SRR935970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541810
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7660.14137797382846384No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7120.13141138037319355No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA7100.1310422472822576No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC5970.11018622764437719No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA5710.10538749746221No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGG250.002055734271.4045942
TCAATAC300.004237361359.4928473
TATTAGG1309.131327E-1041.1949582
GTATGAG1053.2272874E-739.7168771
TAACAAC1508.367351E-1139.7168731
CTAGGGC758.188718E-539.6618964
GTCTATG1900.037.626521
GTGGAAT1751.0913936E-1137.4473421
GTATTAG1601.6734703E-1037.234571
ATTAGGG1452.6666385E-936.9265983
GGAAACA4050.036.7748871
TAGGGCC650.001939140136.6109855
TATGAGG1156.645496E-736.2197232
TATATGG1001.06068455E-535.7022972
TATGGGC2300.033.6263924
GAAAACA3550.033.5635571
GTATATG1104.1814183E-632.495631
TACATGG2203.6379788E-1232.4566352
TCGTAGG1102.0394682E-532.4506459
TCTATGG2056.91216E-1131.9288862
Produced by FastQC (version 0.11.5)