##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935970_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 541810 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.37820269098023 31.0 31.0 34.0 20.0 34.0 2 29.26436942839741 31.0 31.0 34.0 16.0 34.0 3 29.317332644284896 31.0 30.0 34.0 16.0 34.0 4 32.51429652461195 37.0 35.0 37.0 18.0 37.0 5 32.67427142356176 37.0 35.0 37.0 19.0 37.0 6 32.8618408667245 37.0 35.0 37.0 22.0 37.0 7 32.82655543456193 37.0 35.0 37.0 22.0 37.0 8 32.68523467636256 37.0 35.0 37.0 17.0 37.0 9 33.979882246543994 39.0 35.0 39.0 15.0 39.0 10-11 33.99875417581809 39.0 35.0 39.0 16.0 39.0 12-13 33.9628098410882 39.0 35.0 39.0 17.0 39.0 14-15 35.00073088352005 40.0 36.0 41.0 17.0 41.0 16-17 35.05268913456747 40.0 36.0 41.0 19.0 41.0 18-19 35.19277698824311 40.0 36.0 41.0 22.5 41.0 20-21 35.31994702940145 40.0 36.0 41.0 24.0 41.0 22-23 35.09187630350122 40.0 35.0 41.0 23.0 41.0 24-25 34.82126760303427 40.0 35.0 41.0 21.5 41.0 26-27 34.643669367490446 40.0 35.0 41.0 19.5 41.0 28-29 34.47766006533655 40.0 35.0 41.0 15.5 41.0 30-31 34.27755393957291 40.0 35.0 41.0 8.5 41.0 32-33 33.981584872925936 39.5 34.0 41.0 2.0 41.0 34-35 33.74353371107953 39.0 34.0 41.0 2.0 41.0 36-37 33.58749284804637 39.0 34.0 41.0 2.0 41.0 38-39 33.450014765323644 39.0 34.0 41.0 2.0 41.0 40-41 33.219503146859594 39.0 34.0 41.0 2.0 41.0 42-43 32.95944980712796 39.0 33.5 41.0 2.0 41.0 44-45 32.642748380428564 38.0 33.0 41.0 2.0 41.0 46-47 32.314393422048326 38.0 33.0 40.5 2.0 41.0 48-49 31.94901718314538 37.0 33.0 40.0 2.0 41.0 50-51 31.575531090234584 36.5 32.0 40.0 2.0 41.0 52-53 31.235487532529852 36.0 32.0 39.0 2.0 41.0 54-55 30.8593464498625 35.0 32.0 39.0 2.0 41.0 56-57 30.517606725604917 35.0 31.5 38.5 2.0 41.0 58-59 30.173415957623522 35.0 31.0 37.0 2.0 40.0 60-61 29.861225337295362 35.0 31.0 37.0 2.0 39.5 62-63 29.532566766947824 35.0 31.0 36.0 2.0 39.0 64-65 29.294235063952307 35.0 31.0 35.5 2.0 38.5 66-67 29.142201140621253 35.0 30.5 35.0 2.0 37.0 68-69 29.035997859028072 35.0 31.0 35.0 2.0 37.0 70-71 28.821853601816134 35.0 31.0 35.0 2.0 36.0 72-73 28.63142614569683 35.0 30.5 35.0 2.0 36.0 74-75 28.396001365792436 35.0 30.0 35.0 2.0 35.5 76-77 28.122907476790758 35.0 29.0 35.0 2.0 35.0 78-79 27.818221332201325 35.0 29.0 35.0 2.0 35.0 80-81 27.445838947232424 35.0 27.5 35.0 2.0 35.0 82-83 27.063672689688268 34.5 25.5 35.0 2.0 35.0 84-85 26.687176316420885 34.0 25.0 35.0 2.0 35.0 86-87 26.355219541905832 34.0 23.5 35.0 2.0 35.0 88-89 25.91801092633949 34.0 19.5 35.0 2.0 35.0 90-91 25.457139956811428 34.0 16.0 35.0 2.0 35.0 92-93 24.965856111921156 34.0 8.5 35.0 2.0 35.0 94-95 24.4982853767926 33.0 6.0 35.0 2.0 35.0 96-97 24.04765877337074 33.0 2.0 35.0 2.0 35.0 98-99 23.542486295933998 33.0 2.0 35.0 2.0 35.0 100-101 23.10249072553109 32.5 2.0 35.0 2.0 35.0 102-103 22.236589394806295 30.5 2.0 34.5 2.0 35.0 104-105 22.465456525350213 31.0 2.0 35.0 2.0 35.0 106-107 22.271261143205184 31.0 2.0 35.0 2.0 35.0 108-109 21.932515088315093 31.0 2.0 35.0 2.0 35.0 110-111 21.502838633469295 31.0 2.0 35.0 2.0 35.0 112-113 21.022435909267088 30.0 2.0 35.0 2.0 35.0 114-115 20.45953655340433 29.5 2.0 35.0 2.0 35.0 116-117 19.835885273435338 29.0 2.0 35.0 2.0 35.0 118-119 19.018415127074064 26.0 2.0 35.0 2.0 35.0 120-121 18.12361067532899 22.5 2.0 35.0 2.0 35.0 122-123 17.20895424595338 6.0 2.0 35.0 2.0 35.0 124-125 14.892557354054006 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5331008828495705 1101 2 0.568621033546723 1101 3 0.5360315442687096 1101 4 0.6100830491417 1101 5 0.5938505979761928 1101 6 0.7405388020137949 1101 7 0.47386456039627234 1101 8 0.45254321218828153 1101 9 0.44905723875591264 1101 10-11 0.7449557731059357 1101 12-13 0.607716493745329 1101 14-15 0.7044648393288142 1101 16-17 0.5988455226801577 1101 18-19 0.5464904179191379 1101 20-21 0.48769080108334606 1101 22-23 0.7325092220867049 1101 24-25 0.7248661578117392 1101 26-27 0.6831919216971158 1101 28-29 0.5953287200979318 1101 30-31 0.33439983956339603 1101 32-33 0.46637532351594047 1101 34-35 0.1720711397769179 1101 36-37 0.23929784404271714 1101 38-39 0.42058935722942437 1101 40-41 0.4338889737433149 1101 42-43 0.41510091402307125 1101 44-45 0.6716488371081653 1101 46-47 0.6152227075737642 1101 48-49 0.5657481734983847 1101 50-51 0.6711367356614275 1101 52-53 0.5521474770046808 1101 54-55 0.4951746733910305 1101 56-57 0.23218010998938965 1101 58-59 0.36788646062590047 1101 60-61 0.6215688997377598 1101 62-63 0.5642227336969228 1101 64-65 0.26998438327854046 1101 66-67 0.34342937644597527 1101 68-69 0.40680196412258596 1101 70-71 0.29627931443560485 1101 72-73 0.43202389515967354 1101 74-75 0.6089530552141262 1101 76-77 0.5534173591856835 1101 78-79 0.6134012735118013 1101 80-81 0.5794276685747306 1101 82-83 0.6539991248825778 1101 84-85 0.7556608743464004 1101 86-87 0.4423889880432945 1101 88-89 0.5404748408645581 1101 90-91 0.2711728675713303 1101 92-93 0.10506283238696668 1101 94-95 0.14457371535254282 1101 96-97 0.2086311120873745 1101 98-99 0.4326567832003576 1101 100-101 0.6221053190405108 1101 102-103 0.6547389298153661 1101 104-105 0.6611034125260957 1101 106-107 0.7524257596551784 1101 108-109 0.7361122786712606 1101 110-111 0.6610620560483511 1101 112-113 0.5973873027226055 1101 114-115 0.22536742293219802 1101 116-117 0.17066962069865 1101 118-119 0.19406155726571583 1101 120-121 -0.04369154478006365 1101 122-123 0.07353447003915292 1101 124-125 0.3223276872449876 1102 1 0.969030560710042 1102 2 1.1920679029226413 1102 3 1.1703002270253258 1102 4 0.9896049088903638 1102 5 1.1605556426544865 1102 6 1.1421915451902365 1102 7 1.1115263571159346 1102 8 1.087177337444679 1102 9 1.2168185301432644 1102 10-11 1.3037762976141636 1102 12-13 1.2622770589630647 1102 14-15 1.327656291903807 1102 16-17 1.5145363661843163 1102 18-19 1.342982169921207 1102 20-21 1.2172024441599802 1102 22-23 1.237273727796257 1102 24-25 1.2641875743140218 1102 26-27 1.1094128140055375 1102 28-29 1.2092662255009756 1102 30-31 1.1568900747826234 1102 32-33 1.371422163027738 1102 34-35 1.495152763127514 1102 36-37 1.3625688687749218 1102 38-39 1.211223223139534 1102 40-41 1.200551972886693 1102 42-43 1.1716169895635744 1102 44-45 1.0569696406101272 1102 46-47 1.0763639884300744 1102 48-49 1.1477232133826973 1102 50-51 1.1813769846338644 1102 52-53 1.1276339477732122 1102 54-55 1.2292003172378436 1102 56-57 1.028444933987565 1102 58-59 0.9555199650230009 1102 60-61 0.7896852204783933 1102 62-63 0.9648655981374077 1102 64-65 0.7221143629237865 1102 66-67 0.8010341169817998 1102 68-69 1.034111521426329 1102 70-71 0.932934179536236 1102 72-73 0.7733265601392816 1102 74-75 0.8652608678246452 1102 76-77 0.8193799268711359 1102 78-79 1.0057087088913512 1102 80-81 1.0685007413567291 1102 82-83 1.0907264890597794 1102 84-85 0.9878710941620277 1102 86-87 1.2104724975487287 1102 88-89 1.1540753925648488 1102 90-91 1.17489546920757 1102 92-93 1.391127684677464 1102 94-95 1.4189591432346234 1102 96-97 1.4469683880082513 1102 98-99 1.5506279093133841 1102 100-101 1.51095480388447 1102 102-103 1.5959989598366633 1102 104-105 1.8413500665660045 1102 106-107 1.8978453505167607 1102 108-109 1.9289951877507399 1102 110-111 1.8032732189413991 1102 112-113 1.531943624091955 1102 114-115 1.4985769511577196 1102 116-117 1.522684073360093 1102 118-119 1.293557207321122 1102 120-121 1.3202272955490706 1102 122-123 1.2995026961891192 1102 124-125 0.8264782102600066 1103 1 0.1564898810763502 1103 2 0.07730592608164955 1103 3 0.17554127750591064 1103 4 0.09892149104430814 1103 5 0.14681538684581596 1103 6 0.2515308322560017 1103 7 0.15038202955973645 1103 8 0.2778418345308822 1103 9 0.4370675273429896 1103 10-11 0.4205476591520423 1103 12-13 0.28435380156504664 1103 14-15 0.2007888129058557 1103 16-17 0.17206304445571163 1103 18-19 0.0807253016853835 1103 20-21 0.02614439526873724 1103 22-23 -0.15632727121986534 1103 24-25 0.002110453296843673 1103 26-27 0.06034560746651607 1103 28-29 0.10255313990346338 1103 30-31 0.20305862091143467 1103 32-33 -0.08865692574847372 1103 34-35 0.032579918202067404 1103 36-37 0.09687788262491637 1103 38-39 0.051855554933972314 1103 40-41 0.15552813272926613 1103 42-43 0.12292062991777186 1103 44-45 0.11592483625078387 1103 46-47 0.13455667744846522 1103 48-49 0.07673630654852559 1103 50-51 0.1270516989303907 1103 52-53 0.18754274971069762 1103 54-55 0.07822968225713822 1103 56-57 0.13461877792914123 1103 58-59 0.3336437824755052 1103 60-61 0.27654853690917847 1103 62-63 -0.020202138182884966 1103 64-65 -0.011017779663060168 1103 66-67 0.2581558909922421 1103 68-69 0.39908162662575464 1103 70-71 0.3223934242195021 1103 72-73 0.17525836972075126 1103 74-75 0.2855874401397891 1103 76-77 0.38414379578811264 1103 78-79 0.3939835140075232 1103 80-81 0.4538503969989023 1103 82-83 0.33525460530892914 1103 84-85 0.39325026618957537 1103 86-87 0.21329547491950152 1103 88-89 0.19042028017553392 1103 90-91 0.10535221635053205 1103 92-93 0.02864376732334506 1103 94-95 0.1657861318064846 1103 96-97 -0.035007696151289736 1103 98-99 0.12604227395426904 1103 100-101 0.014422979166390348 1103 102-103 0.08394643580729877 1103 104-105 -0.07666817304649953 1103 106-107 -0.16625250765322264 1103 108-109 0.011909741599222201 1103 110-111 -0.037245588293899345 1103 112-113 0.08487634186989723 1103 114-115 -0.15354974225028428 1103 116-117 0.0932187740336694 1103 118-119 -0.3887537742879026 1103 120-121 -0.43776294133884264 1103 122-123 -0.27071035156687984 1103 124-125 -0.15095121530572442 1104 1 0.4960072538423823 1104 2 0.633996482191602 1104 3 0.6850033114349685 1104 4 0.7690385190766023 1104 5 0.8731512300826836 1104 6 0.7502154008852315 1104 7 0.7073991755272573 1104 8 0.6595255866854686 1104 9 0.7912632998187235 1104 10-11 0.9003625915660862 1104 12-13 0.7839319563323528 1104 14-15 1.1443205193378532 1104 16-17 1.156110037107446 1104 18-19 1.0167409054230205 1104 20-21 1.0927777981536124 1104 22-23 1.2438541675898946 1104 24-25 0.9758199697624121 1104 26-27 1.2135207866373463 1104 28-29 0.9990196190833558 1104 30-31 1.2317894564371556 1104 32-33 1.3168769579492547 1104 34-35 1.2373109351665903 1104 36-37 1.3224834346524972 1104 38-39 1.2576933486380497 1104 40-41 1.3769560561620935 1104 42-43 1.1346415770283613 1104 44-45 1.0436486863823262 1104 46-47 1.112765876396125 1104 48-49 1.1878040739439726 1104 50-51 1.130961705280754 1104 52-53 0.9333152254432591 1104 54-55 0.967397785314386 1104 56-57 1.027057315532332 1104 58-59 1.026368086204073 1104 60-61 0.8557375054295484 1104 62-63 0.96948306548218 1104 64-65 0.7771297300892783 1104 66-67 0.8633223611002023 1104 68-69 0.8599997070031442 1104 70-71 1.06040939083476 1104 72-73 0.9397099709182477 1104 74-75 0.9949496827234761 1104 76-77 1.0848559431164233 1104 78-79 1.2677746779374885 1104 80-81 0.9896845619267793 1104 82-83 1.1667024870108271 1104 84-85 1.179202366343798 1104 86-87 1.301506488255253 1104 88-89 1.343586387405871 1104 90-91 1.282894627672313 1104 92-93 1.1700435677406524 1104 94-95 1.034233922788978 1104 96-97 0.8586419455070597 1104 98-99 1.0482277044178474 1104 100-101 0.797816792102978 1104 102-103 1.0770699049173444 1104 104-105 0.6803671168020031 1104 106-107 0.6738862930362437 1104 108-109 0.9916566344596056 1104 110-111 0.7096880958211322 1104 112-113 0.29052362482372374 1104 114-115 0.12216920903339457 1104 116-117 -0.05133352570323879 1104 118-119 -0.16366078689789632 1104 120-121 -0.3652736008689139 1104 122-123 -0.24120840109967645 1104 124-125 0.05126233817336612 1105 1 0.28168976210995567 1105 2 0.17763658267116256 1105 3 0.1406561325593607 1105 4 0.21252214885847565 1105 5 0.10504418720971387 1105 6 0.3710640641180021 1105 7 0.2502053294775095 1105 8 0.3396081355508471 1105 9 0.21556785100155196 1105 10-11 0.2849049782094113 1105 12-13 0.3726102441200254 1105 14-15 0.4601206766543413 1105 16-17 0.37728094539869517 1105 18-19 0.5998141590831665 1105 20-21 0.518422076823704 1105 22-23 0.5994714656369737 1105 24-25 0.5061710561765267 1105 26-27 0.5405910631252908 1105 28-29 0.4860579248017274 1105 30-31 0.45190482185966374 1105 32-33 0.4548392865715485 1105 34-35 0.4619444977083518 1105 36-37 0.5317236636917713 1105 38-39 0.4990126080664652 1105 40-41 0.5610933105665268 1105 42-43 0.51285747275967 1105 44-45 0.4581404103510991 1105 46-47 0.4144566057187262 1105 48-49 0.34814763284884265 1105 50-51 0.21611728790150764 1105 52-53 0.2166658974214677 1105 54-55 0.2426517330277953 1105 56-57 0.2329803657805698 1105 58-59 0.16811954307313925 1105 60-61 0.3104819507197796 1105 62-63 0.3109985476233774 1105 64-65 0.33471517577703125 1105 66-67 0.4408244121350222 1105 68-69 0.45094047650066216 1105 70-71 0.35417931970402705 1105 72-73 0.44386786253902955 1105 74-75 0.3986719937533927 1105 76-77 0.5444501342474979 1105 78-79 0.4613272394051009 1105 80-81 0.5194393681145613 1105 82-83 0.5003318459884376 1105 84-85 0.8070879820348473 1105 86-87 0.8333089461138847 1105 88-89 1.0945552370502192 1105 90-91 0.9564561544880092 1105 92-93 1.035086322301563 1105 94-95 0.887954119350006 1105 96-97 0.6386010093893972 1105 98-99 0.7653143046056101 1105 100-101 0.7745508680377853 1105 102-103 0.44506192068282147 1105 104-105 0.4834221014509552 1105 106-107 0.6420567699758486 1105 108-109 0.9138035815894234 1105 110-111 0.7559881981614289 1105 112-113 1.052231439054946 1105 114-115 1.2615406197224566 1105 116-117 1.1238823615701925 1105 118-119 1.0284158752860968 1105 120-121 0.8822452813382498 1105 122-123 1.0120237760420672 1105 124-125 0.8768431827768168 1106 1 -0.0031122813127772986 1106 2 0.12655101434421567 1106 3 0.1815245872209168 1106 4 -0.6163411972462498 1106 5 -0.10696092134711677 1106 6 0.17055320843473254 1106 7 0.05735730904328307 1106 8 -0.17124754771863593 1106 9 0.13383094167843979 1106 10-11 0.19231238561681607 1106 12-13 0.45626286225540014 1106 14-15 0.4320236140745237 1106 16-17 0.4315593617460749 1106 18-19 0.42740036597971454 1106 20-21 0.39198529521450354 1106 22-23 0.2763552459690217 1106 24-25 0.5834379782710357 1106 26-27 0.49078263400652133 1106 28-29 0.20636443821168626 1106 30-31 0.16135565200015378 1106 32-33 0.35586099793808756 1106 34-35 0.3457248297645421 1106 36-37 0.41103400851935135 1106 38-39 0.6471607562146104 1106 40-41 0.6128174484975659 1106 42-43 0.41387918412620905 1106 44-45 0.36682495696668127 1106 46-47 0.3869981127429938 1106 48-49 0.6080454617121873 1106 50-51 0.6784416812603808 1106 52-53 0.5097693456973289 1106 54-55 0.44443972791924224 1106 56-57 0.38495993155324015 1106 58-59 0.47335581382665026 1106 60-61 0.337940443695512 1106 62-63 0.5166179601393388 1106 64-65 0.5646002332482958 1106 66-67 0.42677588084510987 1106 68-69 0.34749220063859454 1106 70-71 0.15941559045753806 1106 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2216 74-75 -2.217408764568745 2216 76-77 -2.3610809566284203 2216 78-79 -2.5382809110651223 2216 80-81 -2.4975076057289627 2216 82-83 -2.4294565967310398 2216 84-85 -2.2505201186767003 2216 86-87 -2.0467146063234587 2216 88-89 -2.0235303810353997 2216 90-91 -2.4176938015584994 2216 92-93 -2.258160182151837 2216 94-95 -2.152696641128344 2216 96-97 -2.170186786467365 2216 98-99 -2.134888461976466 2216 100-101 -2.0963046493694577 2216 102-103 -2.0300234983681165 2216 104-105 -2.37467861009803 2216 106-107 -2.2129568307274106 2216 108-109 -1.997136691419815 2216 110-111 -2.0437227868943886 2216 112-113 -2.360160128509037 2216 114-115 -2.367313603959353 2216 116-117 -2.291697907458353 2216 118-119 -2.4520061611704236 2216 120-121 -1.9051649457271473 2216 122-123 -1.5612665989724448 2216 124-125 -1.27375557989091 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32597.0 3 3528.0 4 2065.0 5 3786.0 6 8639.0 7 8182.0 8 5293.0 9 4994.0 10 4007.0 11 2966.0 12 2349.0 13 2069.0 14 1982.0 15 2074.0 16 1979.0 17 1993.0 18 2333.0 19 2897.0 20 3776.0 21 4808.0 22 6237.0 23 7599.0 24 9070.0 25 9914.0 26 10610.0 27 11305.0 28 13046.0 29 15660.0 30 20319.0 31 26538.0 32 32828.0 33 38427.0 34 45727.0 35 56112.0 36 88976.0 37 42742.0 38 4382.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.58995763307134 18.12535583044356 10.852551258068571 15.432135278416526 2 19.349949788817675 24.5204093688159 34.565599432908996 21.564041409457424 3 30.97026771607035 23.632255873362766 24.116965195434208 21.280511215132677 4 16.306424548520543 22.17164249361754 28.63994063342803 32.88199232443388 5 16.581886909464078 34.06632754763786 25.61314590731567 23.738639635582395 6 31.903988483047563 36.00247319170927 10.234030379653385 21.859507945589783 7 29.358500873924203 39.42588624404541 12.021764127210881 19.193848754819502 8 35.94834332457254 28.08910167439388 11.297729084841862 24.664825916191713 9 36.64716413502889 16.65030176630184 14.838781122533728 31.863752976135544 10-11 30.436038463668076 27.316586995441206 15.300843469112788 26.94653107177793 12-13 32.61410826673557 28.217364020597625 17.624997692918182 21.54353001974862 14-15 35.27509643602001 22.849707462025435 20.64441409350141 21.230782008453147 16-17 30.320222956386928 24.230634355216772 21.705118030305826 23.744024658090474 18-19 29.126169690481902 24.494010815599566 21.851756150680128 24.528063343238404 20-21 37.37574057326369 22.54831029327624 18.033074324947858 22.04287480851221 22-23 33.85476458537126 25.129934848009448 17.491925213635774 23.523375352983518 24-25 27.373618057990807 27.06520736051383 18.97390229047083 26.587272291024526 26-27 24.82080812834817 26.48089958149343 21.21057755752735 27.48771473263105 28-29 26.639933181669257 25.094897719039995 22.801017041443057 25.464152057847695 30-31 27.453443088905704 26.275077979365456 20.626418855318285 25.645060076410548 32-33 24.462357653051807 26.467211753197617 22.1221461397907 26.948284453959875 34-35 23.920101142466915 26.700042450305457 21.99202672523578 27.387829681991843 36-37 24.61693213488123 27.639670733282884 20.210590428378953 27.53280670345693 38-39 25.192410623650357 25.2221258374707 21.57592144847825 28.009542090400696 40-41 23.811022314095347 25.32428342038722 23.157656743138737 27.70703752237869 42-43 23.44862590206899 25.496945423672507 24.28803455085731 26.766394123401195 44-45 23.69068492645023 25.057861612004206 24.998892600727192 26.25256086081837 46-47 21.83883649249737 25.33120466584227 24.69075875306842 28.13920008859194 48-49 20.95402447352393 25.702091138960153 25.311917461840867 28.03196692567505 50-51 21.8617227441354 25.93787490079548 24.41381665159373 27.786585703475385 52-53 21.679740130303983 25.454864251305807 24.138258799210057 28.727136819180153 54-55 20.42311880548532 24.95367379708754 24.088702681751904 30.534504715675236 56-57 20.970674167464917 24.54545790121611 24.61531646761128 29.86855146370769 58-59 22.41837544526679 23.31426145696831 24.390930399955703 29.876432697809197 60-61 22.43037227072221 23.22308558350713 23.995127443199646 30.351414702571013 62-63 22.201602037614663 22.791107583839356 24.481091157416806 30.52619922112918 64-65 22.13999699153477 22.433737630455266 24.97939755022337 30.44686782778659 66-67 22.86511876857201 23.226315498052823 23.855410568280394 30.053155165094775 68-69 22.855579222485964 23.092853530412828 24.229020004503692 29.82254724259752 70-71 22.60435434326727 22.6649031219541 25.40935035590867 29.321392178869953 72-73 23.38746367496325 21.975343732437324 26.20902734263611 28.428165249963318 74-75 23.695610843776013 21.72133038741747 26.28150108810614 28.301557680700373 76-77 23.981122516308776 21.208838161752425 26.38030525489125 28.42973406704755 78-79 24.367902223485487 21.524915698763582 26.95461668084761 27.152565396903327 80-81 24.84470903303861 21.933701300558965 27.07705994125198 26.144529725150445 82-83 24.37478082722726 22.041213709602996 27.018788874328642 26.565216588841107 84-85 24.129012964429506 21.89969596446827 27.338369210264474 26.63292186083775 86-87 24.839492911109943 21.46185343813244 27.059229584430373 26.639424066327244 88-89 25.252506606290904 21.237413896700964 26.83174010117801 26.67833939583012 90-91 25.872907476790758 21.05507465716764 26.51446078883741 26.557557077204187 92-93 26.45787051180408 21.33401508655897 26.13003827478285 26.078076126854093 94-95 25.7805109235073 21.88836250298803 25.973129399560126 26.357997173944543 96-97 25.656376232854015 22.193817987509725 25.18053864888336 26.969267130752893 98-99 25.345030135631102 22.84595529166178 24.91876622483737 26.890248347869743 100-101 25.242083212210982 24.07441757450755 24.79561467864509 25.88788453463638 102-103 24.52197264723796 25.384452114209775 25.283863346929735 24.809711891622523 104-105 23.629311490417273 26.74000753034285 25.12476744529427 24.505913533945602 106-107 22.97282706496311 27.95826931151747 24.72547906775008 24.343424555769346 108-109 22.731677156198668 29.62099259888153 24.578265443605694 23.069064801314113 110-111 22.39751942562891 31.60360643029844 23.76543437736476 22.233439766707885 112-113 22.080720551118247 33.000927451170384 23.069448097340846 21.84890390037052 114-115 21.59700323099541 33.98110130707192 23.182507403787675 21.239388058144996 116-117 21.29058156918477 34.7689226850741 22.865487901662945 21.075007844078183 118-119 21.189182200591958 35.62218045980727 22.62013492235731 20.568502417243465 120-121 21.45899220292153 35.82318747225711 22.019433172974434 20.698387151846926 122-123 22.00449623191402 36.046545967326644 21.762334644426684 20.186623156332654 124-125 22.208705868204756 37.680192785350535 21.09103187341292 19.02006947303179 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6.0 1 5.0 2 8.0 3 12.5 4 12.0 5 12.5 6 13.5 7 12.5 8 14.5 9 14.0 10 11.5 11 17.5 12 25.0 13 34.5 14 48.5 15 61.0 16 69.5 17 92.5 18 125.5 19 170.0 20 249.0 21 340.0 22 477.5 23 682.5 24 912.0 25 1173.0 26 1453.5 27 1761.5 28 2107.0 29 2412.0 30 2650.0 31 2845.0 32 2971.0 33 3025.0 34 3070.0 35 3030.5 36 2872.0 37 2646.5 38 2423.0 39 2256.0 40 2110.5 41 2000.5 42 1972.0 43 2137.5 44 2610.0 45 3679.5 46 5408.0 47 7551.5 48 10760.5 49 16289.0 50 24354.0 51 34420.5 52 44453.0 53 51201.0 54 52498.5 55 48932.5 56 42333.5 57 33981.5 58 26255.5 59 19795.5 60 14524.5 61 10989.5 62 8463.5 63 6578.5 64 5535.0 65 4685.0 66 3796.0 67 3160.0 68 2729.5 69 2321.5 70 1696.5 71 1149.5 72 779.5 73 559.5 74 408.0 75 311.5 76 258.0 77 194.0 78 159.5 79 141.5 80 108.0 81 90.0 82 75.0 83 55.0 84 43.0 85 28.5 86 20.5 87 20.0 88 19.0 89 14.5 90 9.5 91 7.0 92 4.0 93 2.5 94 2.0 95 3.0 96 1.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15171370037467008 2 0.01808752145586091 3 0.0077517949096546765 4 0.01679555563758513 5 0.0025839316365515587 6 0.0 7 1.8456654546796848E-4 8 3.6913309093593695E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.614163636699212E-4 28-29 0.00784407818238866 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.8456654546796848E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0010151160000738267 66-67 0.0 68-69 0.005906129454974991 70-71 0.01808752145586091 72-73 9.228327273398424E-5 74-75 0.007936361455122644 76-77 9.228327273398424E-5 78-79 5.536996364039054E-4 80-81 8.30549454605858E-4 82-83 0.0 84-85 0.01790295491039294 86-87 0.0021225152728816376 88-89 0.016887838910319114 90-91 0.0 92-93 0.012827374910023809 94-95 0.012089108728151936 96-97 0.0052601465458371015 98-99 0.0017533821819457006 100-101 4.614163636699212E-4 102-103 0.0 104-105 3.6913309093593695E-4 106-107 4.614163636699212E-4 108-109 0.0 110-111 0.0 112-113 4.614163636699212E-4 114-115 0.004891013454901165 116-117 0.0 118-119 0.07151953636883779 120-121 8.30549454605858E-4 122-123 0.004245030545763275 124-125 0.24224359092670864 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 541810.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.26831790460169 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39148308465201 51.92647379251325 2 8.006729594897832 9.971311675825618 3 2.924694949415524 5.463475046525665 4 1.5285508458395758 3.807211600083461 5 0.9449080587768164 2.9418917697267433 6 0.6620558568993009 2.4735062740805493 7 0.4664029437819324 2.0329488742538806 8 0.35355995432889753 1.7612466907590596 9 0.27719836959058797 1.5534608580273606 >10 1.3867279384974027 14.243099752104934 >50 0.04200899311926801 1.73971066439868 >100 0.014200220559234679 1.467443161239094 >500 0.0014791896415868833 0.6182198404617457 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 766 0.14137797382846384 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 712 0.13141138037319355 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 710 0.1310422472822576 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 597 0.11018622764437719 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 571 0.10538749746221 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.8456654546796848E-4 0.0 0.0 0.0 0.0 3 1.8456654546796848E-4 0.0 0.0 0.0 0.0 4 3.6913309093593695E-4 0.0 0.0 1.8456654546796848E-4 0.0 5 7.382661818718739E-4 0.0 0.0 1.8456654546796848E-4 0.0 6 9.228327273398424E-4 0.0 0.0 1.8456654546796848E-4 0.0 7 0.00239936509108359 0.0 0.0 1.8456654546796848E-4 0.0 8 0.0033221978184234326 0.0 0.0 1.8456654546796848E-4 0.0 9 0.003875897454827338 0.0 0.0 1.8456654546796848E-4 0.0 10-11 0.006459829091378896 0.0 0.0 3.6913309093593695E-4 0.0 12-13 0.01199682545541795 0.0 0.0 0.0013842490910097637 0.0 14-15 0.018548937819530834 0.0 0.0 0.002491648363817574 0.0 16-17 0.020117753456008566 0.0 0.0 0.002768498182019527 0.0 18-19 0.02131743600155036 0.0 0.0 0.0030453480002214798 0.0 20-21 0.022332552001624185 0.0 0.0 0.0044295970912312434 0.0 22-23 0.023532234547165982 0.0 0.0 0.017072405455787084 0.0 24-25 0.02510105018364371 0.0 0.0 0.03995865709381517 0.0 26-27 0.027038998911057384 0.0 0.0 0.09643602000701354 0.0 28-29 0.028884664365737066 0.0 0.0 0.3009357523855226 0.0 30-31 0.030545763274948783 0.0 0.0 0.7631826655100497 0.0 32-33 0.03229914545689448 0.0 0.0 1.4875140731990921 0.0 34-35 0.035990476366253854 0.0 0.0 2.553016740185674 0.0 36-37 0.03857440800280541 0.0 0.0 4.037577748657278 0.0 38-39 0.04032779018475111 0.0 0.0 6.02425204407449 0.0 40-41 0.04309628836677064 0.0 0.0 8.298573300603532 0.0 42-43 0.04595706982152415 0.0 0.0 10.14820693601078 0.0 44-45 0.04771045200346985 0.0 0.0 11.834960595042542 0.0 46-47 0.0479873018216718 0.0 0.0 13.781860799911408 0.0 48-49 0.0479873018216718 0.0 0.0 15.843930529152287 0.0 50-51 0.04807958509440578 0.0 0.0 17.853860209298464 0.0 52-53 0.04817186836713977 0.0 0.0 19.62754471124564 0.0 54-55 0.048264151639873756 0.0 0.0 21.345120983370556 0.0 56-57 0.04844871818534173 0.0 0.0 23.136985290046326 0.0 58-59 0.04854100145807571 0.0 0.0 24.863605322899172 0.0 60-61 0.04863328473080969 0.0 0.0 26.66765102157583 0.0 62-63 0.048725568003543676 0.0 0.0 28.452317232978352 0.0 64-65 0.048725568003543676 0.0 0.0 30.11046307746258 0.0 66-67 0.048725568003543676 0.0 0.0 31.848433952861704 0.0 68-69 0.048725568003543676 0.0 0.0 33.72326092172533 0.0 70-71 0.048725568003543676 0.0 0.0 35.74961702441816 0.0 72-73 0.04881785127627766 0.0 0.0 37.7070375223787 0.0 74-75 0.04891013454901165 0.0 0.0 39.475923294143705 0.0 76-77 0.04891013454901165 0.0 0.0 41.287997637548216 0.0 78-79 0.04891013454901165 0.0 0.0 43.102655912589285 0.0 80-81 0.049002417821745636 0.0 0.0 44.90559421199313 0.0 82-83 0.049094701094479616 0.0 0.0 46.748214318672595 0.0 84-85 0.049094701094479616 0.0 0.0 48.64601982244698 0.0 86-87 0.049094701094479616 0.0 0.0 50.26845204038317 0.0 88-89 0.049094701094479616 1.8456654546796848E-4 0.0 51.75218249940016 0.0 90-91 0.049094701094479616 1.8456654546796848E-4 0.0 53.42084863697606 0.0 92-93 0.049094701094479616 1.8456654546796848E-4 0.0 55.01125855927354 0.0 94-95 0.049094701094479616 1.8456654546796848E-4 0.0 56.44229526955944 0.0 96-97 0.049094701094479616 1.8456654546796848E-4 0.0 57.80061276093095 0.0 98-99 0.049094701094479616 1.8456654546796848E-4 0.0 59.100699507207324 0.0 100-101 0.049094701094479616 1.8456654546796848E-4 0.0 60.449511821487235 0.0 102-103 0.049094701094479616 1.8456654546796848E-4 0.0 61.750798250309145 0.0 104-105 0.049094701094479616 1.8456654546796848E-4 0.0 62.98314907439878 0.0 106-107 0.049094701094479616 1.8456654546796848E-4 0.0 64.14195013011941 0.0 108-109 0.049094701094479616 1.8456654546796848E-4 0.0 65.23098503165316 0.0 110-111 0.049094701094479616 1.8456654546796848E-4 0.0 66.3077462579133 0.0 112-113 0.04927926763994758 1.8456654546796848E-4 0.0 67.38312323508241 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAAGG 25 0.0020557342 71.404594 2 TCAATAC 30 0.0042373613 59.492847 3 TATTAGG 130 9.131327E-10 41.194958 2 GTATGAG 105 3.2272874E-7 39.716877 1 TAACAAC 150 8.367351E-11 39.716873 1 CTAGGGC 75 8.188718E-5 39.661896 4 GTCTATG 190 0.0 37.62652 1 GTGGAAT 175 1.0913936E-11 37.447342 1 GTATTAG 160 1.6734703E-10 37.23457 1 ATTAGGG 145 2.6666385E-9 36.926598 3 GGAAACA 405 0.0 36.774887 1 TAGGGCC 65 0.0019391401 36.610985 5 TATGAGG 115 6.645496E-7 36.219723 2 TATATGG 100 1.06068455E-5 35.702297 2 TATGGGC 230 0.0 33.626392 4 GAAAACA 355 0.0 33.563557 1 GTATATG 110 4.1814183E-6 32.49563 1 TACATGG 220 3.6379788E-12 32.456635 2 TCGTAGG 110 2.0394682E-5 32.450645 9 TCTATGG 205 6.91216E-11 31.928886 2 >>END_MODULE