FastQCFastQC Report
Thu 26 May 2016
SRR935970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541810
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT73371.3541647440984845No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT39830.7351285505989185TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT23730.4379764123954892RNA PCR Primer, Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT10110.18659677746811612TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG8280.1528210996474779TruSeq Adapter, Index 10 (96% over 28bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA7440.13731750982816854No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC7000.12919658182757793No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT6950.1282737491002381No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT6940.12808918255477014No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG6880.1269817832819623TruSeq Adapter, Index 10 (95% over 24bp)
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6590.12162935346339122No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6270.11572322400841624No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGTG208.4415625E-489.349862
TGTTAGG453.959656E-666.185082
GTGTTAG507.3248284E-659.66581
ATTAGGG1001.8189894E-1259.566573
TATTAGG902.3646862E-1159.566572
CATTATG150.00423322159.511585110-111
CCGATTG150.004237865659.4951142-43
AAGCTAA150.004237865659.4951156-57
TCTACTG453.1519786E-452.9480632
TGGTGTA350.007796575350.9958087
GTCTATG1152.6739144E-1046.6949771
AGTCATT909.717587E-846.2739757
TACGCGG653.4945504E-545.820442
AGTTTAA653.519422E-545.765477
TATATGG705.4123124E-542.547552
CTATACA758.049805E-539.7772031
TCTATGG1401.8681021E-938.2927972
TAGCATT401.1980046E-437.18444414-15
GTATTAG1452.59206E-937.0339471
CTTTACA650.001911695736.717421
Produced by FastQC (version 0.11.5)