##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935970_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 541810 Sequences flagged as poor quality 0 Sequence length 125 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.334362599435224 33.0 31.0 34.0 31.0 34.0 2 32.43515623558074 34.0 31.0 34.0 31.0 34.0 3 32.45317362174932 34.0 31.0 34.0 30.0 34.0 4 35.94353924807589 37.0 35.0 37.0 35.0 37.0 5 35.9218323766634 37.0 35.0 37.0 35.0 37.0 6 35.99855115261808 37.0 35.0 37.0 35.0 37.0 7 35.98319337036968 37.0 35.0 37.0 35.0 37.0 8 36.002613462283826 37.0 35.0 37.0 35.0 37.0 9 37.662413023015446 39.0 37.0 39.0 35.0 39.0 10-11 37.628194385485685 39.0 37.0 39.0 35.0 39.0 12-13 37.57529484505638 39.0 37.0 39.0 35.0 39.0 14-15 38.73241542238054 40.0 38.0 41.0 34.0 41.0 16-17 38.66443956368468 40.0 38.0 41.0 34.5 41.0 18-19 38.74153485539211 40.0 38.0 41.0 34.0 41.0 20-21 38.82892157767483 40.0 38.0 41.0 35.0 41.0 22-23 38.66015023716801 40.0 38.0 41.0 34.0 41.0 24-25 38.3697393920378 40.0 38.0 41.0 34.0 41.0 26-27 38.201256898174634 40.0 37.5 41.0 33.0 41.0 28-29 38.08558996696259 40.0 37.0 41.0 33.0 41.0 30-31 37.86522766283383 40.0 37.0 41.0 33.0 41.0 32-33 37.507605987338735 40.0 35.5 41.0 33.0 41.0 34-35 37.280442406009485 40.0 35.0 41.0 32.0 41.0 36-37 37.16361362839372 40.0 35.0 41.0 32.0 41.0 38-39 36.75222956386925 39.5 35.0 41.0 31.0 41.0 40-41 36.52106273416881 39.0 35.0 41.0 31.0 41.0 42-43 36.212821837913665 39.0 35.0 41.0 30.5 41.0 44-45 35.76334323840461 39.0 35.0 41.0 29.5 41.0 46-47 35.202755578523835 38.0 35.0 41.0 28.0 41.0 48-49 34.47404717520902 37.0 35.0 40.0 25.5 41.0 50-51 33.7579391299533 37.0 34.0 40.0 22.5 41.0 52-53 33.26399660397556 36.0 34.0 39.0 20.5 41.0 54-55 32.83209612225688 35.5 34.0 39.0 18.5 41.0 56-57 32.48590373008989 35.0 33.0 38.5 18.0 41.0 58-59 32.169796607666896 35.0 33.0 37.0 17.0 40.0 60-61 31.779885015042176 35.0 33.0 37.0 12.0 39.5 62-63 31.40285063029475 35.0 33.0 36.0 7.5 39.0 64-65 31.08105516694044 35.0 33.0 35.5 2.0 38.5 66-67 30.670111293626917 35.0 33.0 35.0 2.0 37.0 68-69 30.222987763238034 35.0 32.0 35.0 2.0 37.0 70-71 29.760038574408 35.0 31.5 35.0 2.0 36.0 72-73 29.451542053487387 35.0 31.0 35.0 2.0 36.0 74-75 29.28954891936288 35.0 31.0 35.0 2.0 35.0 76-77 29.142483527435818 35.0 31.0 35.0 2.0 35.0 78-79 29.188350159650064 35.0 31.0 35.0 2.0 35.0 80-81 29.137730938890016 35.0 31.0 35.0 2.0 35.0 82-83 29.01276739078275 35.0 31.0 35.0 2.0 35.0 84-85 28.927401672172902 35.0 31.0 35.0 2.0 35.0 86-87 28.91530610361566 35.0 31.0 35.0 2.0 35.0 88-89 28.841259851239364 35.0 31.0 35.0 2.0 35.0 90-91 28.728594894889355 35.0 30.0 35.0 2.0 35.0 92-93 28.639996493235635 35.0 30.0 35.0 2.0 35.0 94-95 28.60481534117126 35.0 30.0 35.0 2.0 35.0 96-97 28.572654620623467 35.0 30.0 35.0 2.0 35.0 98-99 28.48630054816264 35.0 30.0 35.0 2.0 35.0 100-101 28.39105590520662 35.0 30.0 35.0 2.0 35.0 102-103 27.61026374559347 33.5 28.0 35.0 2.0 35.0 104-105 28.07842509366752 34.0 29.0 35.0 2.0 35.0 106-107 27.970400140270577 35.0 29.0 35.0 2.0 35.0 108-109 27.681619017736843 35.0 29.0 35.0 2.0 35.0 110-111 27.27343718277625 34.0 27.0 35.0 2.0 35.0 112-113 26.734106051937026 34.0 25.0 35.0 2.0 35.0 114-115 26.057098429338698 34.0 21.0 35.0 2.0 35.0 116-117 25.38034089440948 34.0 7.5 35.0 2.0 35.0 118-119 24.787596205311825 34.0 2.0 35.0 2.0 35.0 120-121 24.11461582473561 33.5 2.0 35.0 2.0 35.0 122-123 23.363906166368285 33.0 2.0 35.0 2.0 35.0 124-125 21.020313393994208 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08581612319310494 1101 2 0.16978508608904264 1101 3 0.23291109357999318 1101 4 0.16357669934738794 1101 5 0.2243411344702011 1101 6 0.15153288156878375 1101 7 0.2610328595152325 1101 8 0.2561273327682869 1101 9 0.25186579008734356 1101 10-11 0.2364592234062357 1101 12-13 0.2618849220355486 1101 14-15 0.4601876972502481 1101 16-17 0.11988690769619126 1101 18-19 0.29508512499057105 1101 20-21 0.35383805737859575 1101 22-23 0.34015169056115724 1101 24-25 0.43051465818692947 1101 26-27 0.4126698296622777 1101 28-29 0.5650190278393055 1101 30-31 0.6155177103437595 1101 32-33 0.7159673979025811 1101 34-35 0.6681659631392165 1101 36-37 0.6730193415984402 1101 38-39 0.9353849873086162 1101 40-41 1.0152961621558987 1101 42-43 1.0196292914225609 1101 44-45 1.0400507947583506 1101 46-47 1.106072086366062 1101 48-49 1.3209386688452014 1101 50-51 1.1741428270787608 1101 52-53 1.0281142848280354 1101 54-55 0.8646882984129007 1101 56-57 0.7931953454932312 1101 58-59 0.8817291907641689 1101 60-61 0.8662824013791806 1101 62-63 0.8167098618594935 1101 64-65 0.6061684035729549 1101 66-67 0.37242501681762974 1101 68-69 0.4947324220144438 1101 70-71 0.7776336713760053 1101 72-73 0.6646286907991481 1101 74-75 0.5248035587654591 1101 76-77 0.5159523681928775 1101 78-79 0.4689904775245921 1101 80-81 0.5508645636600278 1101 82-83 0.5186258936556278 1101 84-85 0.4854840370182387 1101 86-87 0.3828944521341491 1101 88-89 0.1386226600959155 1101 90-91 0.30644322300316773 1101 92-93 0.2989522930287549 1101 94-95 0.5253110315646801 1101 96-97 0.5440713564619237 1101 98-99 0.5227458399408924 1101 100-101 0.3512987182999616 1101 102-103 0.2724473877076825 1101 104-105 0.6274235328519175 1101 106-107 0.449799346915956 1101 108-109 0.43290020409460084 1101 110-111 0.3865208875038384 1101 112-113 0.33980321908200395 1101 114-115 0.6473044476385752 1101 116-117 0.33440542270269447 1101 118-119 0.0018033290967629512 1101 120-121 0.17504801860016528 1101 122-123 0.11630532292799245 1101 124-125 0.2733376076304843 1102 1 0.020663226766586718 1102 2 0.06620178484654105 1102 3 0.14322050156314248 1102 4 0.18602390665937918 1102 5 0.1846945527328998 1102 6 0.19350179144987578 1102 7 0.24820504187496795 1102 8 0.2194475154109483 1102 9 0.22537710308688474 1102 10-11 0.24228567358424158 1102 12-13 0.17884990242739462 1102 14-15 0.2736469838121849 1102 16-17 0.3422871576117146 1102 18-19 0.3160391263894624 1102 20-21 0.23141089046174557 1102 22-23 0.02180215463384627 1102 24-25 -0.04223826338832026 1102 26-27 0.17153181028203335 1102 28-29 0.11786279920590204 1102 30-31 0.2563299750655901 1102 32-33 0.21359202165398727 1102 34-35 0.12785431534719294 1102 36-37 0.22652916139205814 1102 38-39 0.33952018531557826 1102 40-41 0.19290514654010593 1102 42-43 0.10189904300025177 1102 44-45 -0.02225420433786951 1102 46-47 0.10776667860296385 1102 48-49 0.3387333608911334 1102 50-51 0.5614715995849124 1102 52-53 0.5113893962290561 1102 54-55 0.7013708664127094 1102 56-57 0.6899588216765977 1102 58-59 0.5987234458508865 1102 60-61 0.5807696423275956 1102 62-63 0.665050242076866 1102 64-65 0.8222117222623915 1102 66-67 0.8488303986461005 1102 68-69 0.9643515759168224 1102 70-71 1.2045107883883368 1102 72-73 1.333360958978929 1102 74-75 1.2136007814024623 1102 76-77 1.112160018122541 1102 78-79 1.2347064697599208 1102 80-81 1.3993807885211176 1102 82-83 1.3689801371110626 1102 84-85 1.4328760350063412 1102 86-87 1.4636071435985158 1102 88-89 1.4008993668695702 1102 90-91 1.3813785829050858 1102 92-93 1.222708413208256 1102 94-95 1.2900279511594697 1102 96-97 1.284617005338479 1102 98-99 1.301967486711245 1102 100-101 1.3447895193656372 1102 102-103 1.2781598826975866 1102 104-105 1.2820951061408188 1102 106-107 1.4600985382270686 1102 108-109 1.312145034851497 1102 110-111 1.148660062153919 1102 112-113 1.091512546903573 1102 114-115 0.8424330598215661 1102 116-117 1.049932995928728 1102 118-119 0.8241157549276537 1102 120-121 0.9486259364424043 1102 122-123 1.2368630353365369 1102 124-125 1.1203665208123503 1103 1 0.024458731194044958 1103 2 -0.012005283949342527 1103 3 0.2244132662645697 1103 4 0.06010624980628165 1103 5 0.24384463220008712 1103 6 0.18352298270091438 1103 7 0.19966572453598275 1103 8 0.25319079854119764 1103 9 0.31397923734859745 1103 10-11 0.22549349921051487 1103 12-13 0.22175650955673376 1103 14-15 0.29120544896014167 1103 16-17 0.48312761776458757 1103 18-19 0.361921969044424 1103 20-21 0.2465512824998939 1103 22-23 0.325794434963818 1103 24-25 0.293438721399383 1103 26-27 0.3814065548517789 1103 28-29 0.5688996568461064 1103 30-31 0.480690453382401 1103 32-33 0.517243728306525 1103 34-35 0.6670562376964 1103 36-37 0.7410565208108153 1103 38-39 0.834474018661524 1103 40-41 0.8725956029088309 1103 42-43 0.5778621096770138 1103 44-45 0.7012094299616862 1103 46-47 0.8765303961538606 1103 48-49 0.8359974190157047 1103 50-51 0.8148148978517682 1103 52-53 0.762560591264247 1103 54-55 0.8435187510717554 1103 56-57 0.8006925687438766 1103 58-59 0.8712467183855139 1103 60-61 0.5969279712460889 1103 62-63 0.570777556085801 1103 64-65 0.6608138565241042 1103 66-67 0.678248396072874 1103 68-69 0.6785951239377113 1103 70-71 0.6725517329126696 1103 72-73 0.5589456104585295 1103 74-75 0.49226005635894765 1103 76-77 0.5573200877509379 1103 78-79 0.5059582672321064 1103 80-81 0.7252151940308451 1103 82-83 0.6365140052712093 1103 84-85 0.7340566904214612 1103 86-87 0.8304333753399824 1103 88-89 0.8084846994965282 1103 90-91 0.5654018932812299 1103 92-93 0.6183642067449249 1103 94-95 0.6499481055528697 1103 96-97 0.7796673345597007 1103 98-99 0.7755654584057865 1103 100-101 0.8010220176627669 1103 102-103 0.8022365496165307 1103 104-105 0.5813061104547543 1103 106-107 0.6416016033901677 1103 108-109 0.6699176830010458 1103 110-111 0.9397691970967763 1103 112-113 0.8812196535409775 1103 114-115 0.8823255899055731 1103 116-117 0.6123658272312298 1103 118-119 0.5665171777687945 1103 120-121 0.6173442441696437 1103 122-123 0.31543527479767164 1103 124-125 0.3422691924236432 1104 1 0.0676593117982307 1104 2 0.1245183484556307 1104 3 0.23160731507161358 1104 4 0.11482819483032358 1104 5 0.18835157223455923 1104 6 0.14894981750087766 1104 7 0.07547993119478491 1104 8 0.13326881722939277 1104 9 0.08300763252232457 1104 10-11 0.18842101440601766 1104 12-13 0.23822661751282936 1104 14-15 0.1452469984475755 1104 16-17 0.29928927974408026 1104 18-19 0.28145385691524183 1104 20-21 0.2667590299370701 1104 22-23 0.46801524599530353 1104 24-25 0.39404654141208795 1104 26-27 0.42273831571542075 1104 28-29 0.32525001518775554 1104 30-31 0.47791443959305724 1104 32-33 0.7071103707813577 1104 34-35 0.48307274862808924 1104 36-37 0.47437775822350403 1104 38-39 0.481199048145335 1104 40-41 0.5083345124615875 1104 42-43 0.6203414710123951 1104 44-45 0.8643864235206067 1104 46-47 0.6689417179355885 1104 48-49 0.8396352671514222 1104 50-51 0.6394316083661451 1104 52-53 0.8981452778287036 1104 54-55 0.9030941842569149 1104 56-57 0.9574874521694454 1104 58-59 0.8920350700139252 1104 60-61 0.8153530823773565 1104 62-63 0.8628668239801982 1104 64-65 0.9248753642806129 1104 66-67 1.1195211893233505 1104 68-69 0.8793254151470009 1104 70-71 0.7838190829988498 1104 72-73 0.7983677278494419 1104 74-75 0.7633931417259028 1104 76-77 0.9910722423368057 1104 78-79 1.0037855374852143 1104 80-81 0.7394762844780907 1104 82-83 0.8314065027764244 1104 84-85 1.0334760862302268 1104 86-87 1.0841982306425315 1104 88-89 1.0233400020457424 1104 90-91 1.0867753850711246 1104 92-93 1.0714259866596478 1104 94-95 1.0255672654911798 1104 96-97 1.1231355374082916 1104 98-99 1.038175455684204 1104 100-101 1.0626794611899406 1104 102-103 0.9708430995575625 1104 104-105 1.1221933462344857 1104 106-107 0.964825771039628 1104 108-109 1.017309500110727 1104 110-111 1.1296313036013608 1104 112-113 0.922764605006094 1104 114-115 0.7162228955392465 1104 116-117 1.0022188617953276 1104 118-119 0.7786508981715912 1104 120-121 0.9913622066118108 1104 122-123 1.3450506245209795 1104 124-125 0.9094741275592675 1105 1 0.12293491167305604 1105 2 0.1768066266859023 1105 3 0.18067737165479514 1105 4 0.09528881073281781 1105 5 0.1067026248171743 1105 6 0.09818734001996887 1105 7 0.21231989973149012 1105 8 0.23164913406141352 1105 9 0.2632855583129228 1105 10-11 0.05747533934068372 1105 12-13 0.07156756293415611 1105 14-15 0.09958208256114887 1105 16-17 0.36293394747644214 1105 18-19 0.22476071709809986 1105 20-21 0.25133355650748257 1105 22-23 0.19782333679904696 1105 24-25 0.179687606089459 1105 26-27 0.11146481339373793 1105 28-29 0.10799848642675158 1105 30-31 0.20071179735273148 1105 32-33 0.17001582019056372 1105 34-35 -0.03489008746674216 1105 36-37 -0.10431516168888066 1105 38-39 -0.19524746002074522 1105 40-41 -0.12132049079469454 1105 42-43 -0.03938889191503847 1105 44-45 -0.3396065395269332 1105 46-47 -0.35101294785146564 1105 48-49 -0.09311785591912525 1105 50-51 0.02347582613952426 1105 52-53 -0.027788260185218405 1105 54-55 -0.05377826252190232 1105 56-57 0.03932078127383676 1105 58-59 0.029348679581474357 1105 60-61 0.042868243119279725 1105 62-63 0.15146476195014458 1105 64-65 0.05424533713083335 1105 66-67 0.20356286973406412 1105 68-69 0.5233063680112622 1105 70-71 0.40013094178615916 1105 72-73 0.3468626488951756 1105 74-75 0.43164143112057474 1105 76-77 0.4083592941751242 1105 78-79 0.29735332312881724 1105 80-81 0.38994865682760604 1105 82-83 0.41095197654432525 1105 84-85 0.5024483697720434 1105 86-87 0.6196747896404418 1105 88-89 0.6213549386539654 1105 90-91 0.3417625713896655 1105 92-93 0.3301786191734166 1105 94-95 0.47239513926584564 1105 96-97 0.5262767923878648 1105 98-99 0.5421472740428683 1105 100-101 0.6707651902165246 1105 102-103 0.7330304753325123 1105 104-105 0.7445297497766425 1105 106-107 0.8890761918189369 1105 108-109 1.1265911727401985 1105 110-111 0.9903097943780459 1105 112-113 1.0113050294222887 1105 114-115 0.9568799033120357 1105 116-117 0.8030155936722139 1105 118-119 1.0388303742907752 1105 120-121 1.0478591237161723 1105 122-123 0.9663450177952768 1105 124-125 1.1779118733662806 1106 1 0.12038063325670834 1106 2 0.029935617745927345 1106 3 0.09638618391533527 1106 4 0.016106179826053335 1106 5 0.07349700540466131 1106 6 0.07392169506466928 1106 7 0.007977626423702588 1106 8 0.13714083265655574 1106 9 0.1713315353244127 1106 10-11 0.2343591196727388 1106 12-13 0.18587152206570323 1106 14-15 0.04785794463011683 1106 16-17 0.06344480315971879 1106 18-19 0.19027795847741658 1106 20-21 0.10446254756750761 1106 22-23 0.11736356668410508 1106 24-25 0.1477591258851163 1106 26-27 0.21299738044355365 1106 28-29 0.2401823944727255 1106 30-31 0.14771052021352205 1106 32-33 0.2236556669338654 1106 34-35 0.17392217306965563 1106 36-37 0.10641280765977967 1106 38-39 0.0831688873611185 1106 40-41 0.10409457944795975 1106 42-43 0.29649871983464493 1106 44-45 0.4937267938063954 1106 46-47 0.4171862858650144 1106 48-49 0.503306154297988 1106 50-51 0.37852052601180475 1106 52-53 0.23019385986586371 1106 54-55 0.3900276123184554 1106 56-57 0.43395679659951014 1106 58-59 0.4297318213439283 1106 60-61 0.30851319841941205 1106 62-63 0.13486195224388453 1106 64-65 0.2081406117157627 1106 66-67 0.22846708429345242 1106 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-2.058719724613219 2216 102-103 -2.0722742135928627 2216 104-105 -2.0065400616379954 2216 106-107 -2.12477745134505 2216 108-109 -2.3098234662606494 2216 110-111 -2.1062220262917073 2216 112-113 -1.96219640528604 2216 114-115 -1.9219411479228086 2216 116-117 -1.7669513168119408 2216 118-119 -1.7140141630365164 2216 120-121 -2.0975730303366724 2216 122-123 -2.083925752303081 2216 124-125 -1.8008473941860927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 28.0 5 107.0 6 300.0 7 903.0 8 1338.0 9 1769.0 10 2658.0 11 4678.0 12 6486.0 13 6883.0 14 9144.0 15 11878.0 16 5909.0 17 2874.0 18 3104.0 19 4445.0 20 5657.0 21 6712.0 22 3884.0 23 2606.0 24 3383.0 25 4262.0 26 5541.0 27 6889.0 28 8428.0 29 10625.0 30 13521.0 31 18193.0 32 24262.0 33 32910.0 34 45501.0 35 69033.0 36 141868.0 37 69546.0 38 6473.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.67827926448616 19.766749508128818 11.721327238568618 16.8336439888164 2 19.039691401888135 21.26133941178099 36.98267826985724 22.71629091647364 3 28.414388807884684 22.50106125763644 27.850722578025504 21.233827356453368 4 15.70107135070013 22.366247463903353 27.85770511432299 34.07497607107353 5 15.776919938486648 33.53677785962328 28.289312147041283 22.396990054848793 6 31.68858111051229 35.30335693350363 10.434031494784092 22.57403046119999 7 28.13403222531884 38.476772300252854 12.85192226057105 20.537273213857258 8 35.36590317639025 27.54397297945774 13.293036304239495 23.797087539912514 9 33.322382384968904 14.113250032299145 17.291855078348497 35.27251250438346 10-11 29.407172255956887 25.058230745095145 18.657462948265994 26.877134050681974 12-13 32.8118713202045 25.110555360735315 20.18069064801314 21.896882671047045 14-15 34.24290803049039 20.06505970727746 23.349975083516362 22.342057178715784 16-17 30.195086838559643 22.078219301969327 24.64932356361086 23.077370295860174 18-19 29.10088407375279 21.749598567763606 25.186689060740854 23.96282829774275 20-21 34.506469057418656 19.240877798490246 23.61787342426312 22.634779719827982 22-23 30.989368966981047 22.04472047396689 23.69225374208671 23.273656816965357 24-25 26.551281814658275 22.981764825307767 25.179121832376662 25.287831527657296 26-27 24.66547313633931 22.119469924881415 27.146047507428804 26.069009431350477 28-29 25.399402004392684 19.919344419630498 29.10854358538971 25.572709990587107 30-31 27.247559107436185 21.16092357099352 27.14429412524686 24.447223196323435 32-33 24.9481368007235 20.115261807644746 29.19962717557815 25.7369742160536 34-35 22.86954836566324 20.615990845499343 29.74059172034477 26.773869068492644 36-37 24.882154260718703 21.70853251139698 26.96711024159761 26.442202986286706 38-39 25.038297558184603 18.626548051900112 28.7470700060907 27.58808438382459 40-41 23.486000627526256 18.742455842454 30.144515605101418 27.62702792491833 42-43 24.529170742511212 18.491537623890295 30.831749137151398 26.147542496447095 44-45 23.89047821191931 18.94704785810524 30.785792067329876 26.37668186264558 46-47 22.996437865672466 18.677119285358337 29.707369742160537 28.619073106808663 48-49 22.0184197412377 19.39572913013787 29.702571011978367 28.883280116646056 50-51 22.111164430335357 20.060814676731695 29.03720861556634 28.790812277366605 52-53 21.812997176131855 20.294937339657814 28.7055425333604 29.18652295084993 54-55 20.188627009468263 20.75183182296377 27.12519148779092 31.934349679777043 56-57 20.777671139329286 21.29104298554844 26.23355050663517 31.697735368487105 58-59 20.63020246950038 21.401598346283755 26.514553072110147 31.45364611210572 60-61 21.361455122644468 22.72410992783448 23.81129916391355 32.1031357856075 62-63 19.96041047599712 23.542754840257654 23.942710544286744 32.55412413945848 64-65 19.61527103597202 23.275041066056367 24.07818238866023 33.03150550931138 66-67 20.44517450766874 23.810468614458944 23.15488824495672 32.5894686329156 68-69 20.929384839703953 23.342223288606707 23.180912127867703 32.54747974382163 70-71 21.00801018807331 22.785109171111646 23.765803510455694 32.44107713035935 72-73 21.78697329322087 21.718683671397724 24.032594451929643 32.461748583451765 74-75 22.157398349975082 21.285413705911665 24.09017921411565 32.4670087299976 76-77 22.083848581606098 21.05525922371311 24.574943245787267 32.28594894889352 78-79 22.030047433602185 21.031450139347744 25.25405584983666 31.684446577213414 80-81 21.821579520496115 21.538731289566453 24.916299071630277 31.723390118307154 82-83 21.288920470275556 21.6398737564829 24.88944463926469 32.181761133976856 84-85 21.313190971004595 20.7557077204186 25.373008988390765 32.55809232018604 86-87 21.716376589579372 20.4722135065798 25.31219431165907 32.49921559218176 88-89 21.572968383750762 20.571048891677897 25.204592015651244 32.651390708920104 90-91 21.616618371753933 19.916299071630274 25.22304867019804 33.244033886417746 92-93 21.685923109577157 19.685775456340785 25.21548144183385 33.4128199922482 94-95 21.207618906996917 19.412063269411785 25.63020246950038 33.750115354090916 96-97 21.467396319743084 19.512467470146362 25.47691995348923 33.54321625662132 98-99 21.761133976855355 19.965947472361158 25.38306786511877 32.88985068566472 100-101 22.09409202487957 20.559236632767945 24.92174378472158 32.42492755763091 102-103 21.87012052195419 21.539469555748326 24.717244052343073 31.873165869954413 104-105 21.48336132592606 22.995699599490596 24.420553330503314 31.10038574408003 106-107 21.338199737915506 24.79033240434839 23.74319410863587 30.128273749100238 108-109 21.07011683062328 25.985308502980747 23.645373839537847 29.299200826858122 110-111 20.734562495212767 27.1466421068077 23.457495355805726 28.661300042173803 112-113 20.675774444083515 28.28585033812716 23.00298260638455 28.03539261140478 114-115 20.401415261494847 28.981391932197383 22.911437261908276 27.70575554439949 116-117 19.989405479434357 29.29680254047005 23.11238959042211 27.60140238967348 118-119 19.930731088784363 29.76938971191479 23.254088443830444 27.045790755470406 120-121 20.11926712180861 30.13977250285617 23.122078075484165 26.618882299851055 122-123 20.03444062591131 30.213528189264714 23.103735312900284 26.648295871923693 124-125 19.213646085353574 31.42295646105661 23.142738724801053 26.22065872878876 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 0.5 3 1.5 4 2.0 5 2.5 6 3.0 7 4.0 8 9.5 9 11.0 10 10.5 11 13.0 12 18.0 13 26.0 14 28.5 15 32.5 16 37.0 17 49.5 18 64.0 19 90.5 20 139.5 21 178.5 22 237.0 23 329.0 24 462.5 25 618.0 26 810.0 27 1054.0 28 1344.5 29 1675.5 30 2026.5 31 2361.0 32 2723.5 33 3082.0 34 3257.0 35 3362.0 36 3445.5 37 3352.5 38 3091.5 39 2872.0 40 2760.0 41 2699.5 42 2745.5 43 3050.5 44 3621.0 45 4695.5 46 6335.0 47 7947.0 48 9854.5 49 12840.5 50 16849.5 51 22242.0 52 29949.5 53 39962.0 54 48873.0 55 53946.0 56 53614.5 57 47658.0 58 38505.0 59 27466.5 60 18032.0 61 12303.5 62 8747.5 63 6450.5 64 5214.5 65 4310.0 66 3460.0 67 2856.5 68 2356.0 69 1894.5 70 1331.0 71 831.0 72 488.0 73 312.5 74 214.5 75 147.0 76 126.0 77 95.5 78 60.5 79 44.0 80 34.5 81 26.0 82 18.0 83 8.5 84 3.5 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1878887432863919 2 9.228327273398424E-4 3 0.0 4 0.11516952437201233 5 0.025654749820047618 6 0.0011073992728078109 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 8.30549454605858E-4 112-113 3.6913309093593695E-4 114-115 3.6913309093593695E-4 116-117 0.0037836141820933537 118-119 0.001568815636477732 120-121 1.8456654546796848E-4 122-123 0.0014765323637437478 124-125 0.026854432365589414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 541810.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.20079256353148 #Duplication Level Percentage of deduplicated Percentage of total 1 80.3778433168943 44.36920655639917 2 9.220312204270881 10.179370827179088 3 3.4407697888210973 5.697996581147382 4 1.8099233192636015 3.9963680681027367 5 1.2115206206702134 3.343844923402868 6 0.7857797737366082 2.6025399774391924 7 0.5925512961956758 2.2896510829184438 8 0.4258796324369479 1.880711459774799 9 0.3323974289540831 1.6513741371910977 >10 1.7213924518349584 16.04942429751192 >50 0.05620437775352086 2.030307906189141 >100 0.02107664102094707 2.0576396338563514 >500 0.0030109487172781473 1.1361155113520247 >1k 0.0010036495724260492 1.3604946313605926 >5k 3.345498574753497E-4 1.354954406175196 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 7337 1.3541647440984845 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 3983 0.7351285505989185 TruSeq Adapter, Index 10 (95% over 21bp) ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 2373 0.4379764123954892 RNA PCR Primer, Index 10 (95% over 23bp) CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 1011 0.18659677746811612 TruSeq Adapter, Index 10 (96% over 26bp) TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 828 0.1528210996474779 TruSeq Adapter, Index 10 (96% over 28bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 744 0.13731750982816854 No Hit TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 700 0.12919658182757793 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 695 0.1282737491002381 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 694 0.12808918255477014 No Hit CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG 688 0.1269817832819623 TruSeq Adapter, Index 10 (95% over 24bp) GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 659 0.12162935346339122 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 627 0.11572322400841624 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.6913309093593695E-4 0.0 0.0 0.0 0.0 3 3.6913309093593695E-4 0.0 0.0 0.0 0.0 4 3.6913309093593695E-4 0.0 0.0 1.8456654546796848E-4 0.0 5 3.6913309093593695E-4 0.0 0.0 1.8456654546796848E-4 0.0 6 5.536996364039054E-4 0.0 0.0 1.8456654546796848E-4 0.0 7 0.0011073992728078109 0.0 0.0 1.8456654546796848E-4 0.0 8 0.0012919658182757793 0.0 0.0 1.8456654546796848E-4 0.0 9 0.0014765323637437478 0.0 0.0 1.8456654546796848E-4 0.0 10-11 0.0021225152728816376 0.0 0.0 3.6913309093593695E-4 0.0 12-13 0.004614163636699212 0.0 0.0 0.0010151160000738267 0.0 14-15 0.007382661818718739 0.0 0.0 0.0014765323637437478 0.0 16-17 0.00830549454605858 0.0 0.0 0.0016610989092117163 0.0 18-19 0.008766910909728504 0.0 0.0 0.001937948727413669 0.0 20-21 0.009043760727930455 0.0 0.0 0.0033221978184234326 0.0 22-23 0.00959746036433436 0.0 0.0 0.015872722910245288 0.0 24-25 0.010612576364408186 0.0 0.0 0.03903582436647533 0.0 26-27 0.012273675273619904 0.0 0.0 0.09532862073420573 0.0 28-29 0.013381074546427715 0.0 0.0 0.29890552038537493 0.0 30-31 0.014673040364703493 0.0 0.0 0.7619829829645078 0.0 32-33 0.016057289455713254 0.0 0.0 1.4893597386537718 0.0 34-35 0.017072405455787084 0.0 0.0 2.556523504549565 0.0 36-37 0.018179804728594894 0.0 0.0 4.046067809748805 0.0 38-39 0.020210036728742546 0.0 0.0 6.040586183348406 0.0 40-41 0.021225152728816376 0.0 0.0 8.32561229951459 0.0 42-43 0.02325538472896403 0.0 0.0 10.185950794558977 0.0 44-45 0.0239936509108359 0.0 0.0 11.87888743286392 0.0 46-47 0.0239936509108359 0.0 0.0 13.832616599915099 0.0 48-49 0.0239936509108359 0.0 0.0 15.904745205883982 0.0 50-51 0.0239936509108359 0.0 0.0 17.929993909304002 0.0 52-53 0.0239936509108359 0.0 0.0 19.71226075561544 0.0 54-55 0.024085934183569885 0.0 0.0 21.442295269559438 0.0 56-57 0.02417821745630387 0.0 0.0 23.247817500599844 0.0 58-59 0.024547350547239808 0.0 0.0 24.991140805817537 0.0 60-61 0.024731917092707775 0.0 0.0 26.82582455104188 0.0 62-63 0.024824200365441758 0.0 0.0 28.64454329008324 0.0 64-65 0.024916483638175745 0.0 0.0 30.323452870932613 0.0 66-67 0.025008766910909728 0.0 0.0 32.08634022996992 0.0 68-69 0.025193333456377698 0.0 0.0 33.99448146029051 0.0 70-71 0.02528561672911168 0.0 0.0 36.05018364371274 0.0 72-73 0.025654749820047618 0.0 0.0 38.033258891493325 0.0 74-75 0.025654749820047618 0.0 0.0 39.82964507853306 0.0 76-77 0.025654749820047618 0.0 0.0 41.66617448921208 0.0 78-79 0.025654749820047618 0.0 0.0 43.50454956534578 0.0 80-81 0.025654749820047618 0.0 0.0 45.33480371347889 0.0 82-83 0.025654749820047618 0.0 0.0 47.209538399069785 0.0 84-85 0.025839316365515584 0.0 0.0 49.14148871375575 0.0 86-87 0.026023882910983554 0.0 0.0 50.79695834333069 0.0 88-89 0.026023882910983554 0.0 0.0 52.31234196489544 0.0 90-91 0.026023882910983554 0.0 0.0 54.01219984865543 0.0 92-93 0.026116166183717537 0.0 0.0 55.62955648659124 0.0 94-95 0.026208449456451524 0.0 0.0 57.08320259869696 0.0 96-97 0.026208449456451524 0.0 0.0 58.45969989479707 0.0 98-99 0.026208449456451524 0.0 0.0 59.78184234325686 0.0 100-101 0.026208449456451524 0.0 0.0 61.156493973902286 0.0 102-103 0.026208449456451524 0.0 0.0 62.475867924180065 0.0 104-105 0.026208449456451524 0.0 0.0 63.73230468245326 0.0 106-107 0.026208449456451524 0.0 0.0 64.91491482253926 0.0 108-109 0.026208449456451524 0.0 0.0 66.02803565825658 0.0 110-111 0.026208449456451524 0.0 0.0 67.1319281667005 0.0 112-113 0.026300732729185507 0.0 0.0 68.23831232350824 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATGTG 20 8.4415625E-4 89.34986 2 TGTTAGG 45 3.959656E-6 66.18508 2 GTGTTAG 50 7.3248284E-6 59.6658 1 ATTAGGG 100 1.8189894E-12 59.56657 3 TATTAGG 90 2.3646862E-11 59.56657 2 CATTATG 15 0.004233221 59.511585 110-111 CCGATTG 15 0.0042378656 59.49511 42-43 AAGCTAA 15 0.0042378656 59.49511 56-57 TCTACTG 45 3.1519786E-4 52.948063 2 TGGTGTA 35 0.0077965753 50.995808 7 GTCTATG 115 2.6739144E-10 46.694977 1 AGTCATT 90 9.717587E-8 46.273975 7 TACGCGG 65 3.4945504E-5 45.82044 2 AGTTTAA 65 3.519422E-5 45.76547 7 TATATGG 70 5.4123124E-5 42.54755 2 CTATACA 75 8.049805E-5 39.777203 1 TCTATGG 140 1.8681021E-9 38.292797 2 TAGCATT 40 1.1980046E-4 37.184444 14-15 GTATTAG 145 2.59206E-9 37.033947 1 CTTTACA 65 0.0019116957 36.71742 1 >>END_MODULE