FastQCFastQC Report
Thu 26 May 2016
SRR935969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102795
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT89610.42614710421129975No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT71630.34064186000061825No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA57740.27458691883897385No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC56300.26773889038161114No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT53370.2538050547010051No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT51740.2460534669332959No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT49830.23697031807665514No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG47570.22622271785884976No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT44700.21257421669730048No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC36280.17253227252299916No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG31180.14827883840317294No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA29700.1412405869331057No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA29220.13895791078065148No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC25550.12150494936501181No Hit
GAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTG25110.1194124962252621No Hit
TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA23550.11199379872978583No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA22470.1068577773867638No Hit
GTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA21380.10167420029056566No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG304.2705426E-579.3126759
GGTATCA261550.048.2796061
TGTTAGA1109.644282E-943.2696882
GTATCAA390000.038.685941
AGTAAGG12200.038.5259862
ACTAGGG3800.036.008923
CCATAGG9150.035.8033941
GAGTAGG26900.034.985441
GAGTAAG21250.034.1966441
GCAGAGA18600.033.9046133
ATTACGA452.3977323E-433.0469510-11
CTTAAGG4150.032.9720233
AGTCGTA3800.032.8730167
TAGGGCA2005.2750693E-1132.717265
GTAAGGG16750.032.676753
TATCAAC461850.032.5401042
TAGGGGA13600.031.933744
TACTAGG4100.031.924592
TATATGG2255.456968E-1231.731112
TACGAAC1352.312343E-630.8849451
Produced by FastQC (version 0.11.5)