FastQCFastQC Report
Thu 26 May 2016
SRR935969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102795
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT99110.4713250697286231No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT74740.35543169923839457No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT60450.287474527949705No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA59040.2807691667518707No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT57730.2745393630857977No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG53130.252663716624778No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC52130.247908141307165No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT49770.23668498355759832No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT47220.2245582664976852No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT46730.22222803459205487No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC40870.19436036323084274No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG32160.15293930221443364No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT32150.15289174646125753RNA PCR Primer, Index 31 (95% over 22bp)
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA30210.14366593034508832No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA29000.1379116842107766No Hit
GAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTG26980.12830542206919837No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC26620.1265934149548577No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA26320.1251667423595738No Hit
TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA25860.12297917771347183No Hit
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT24470.11636892802198977No Hit
CTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAGTTT22150.10533599328512765No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG21910.10419465520890053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA280300.055.9463581
GTATCAA435750.042.2759481
AGTAGAC3900.038.186492
GTGCGTA3600.038.0166635
CGTACTT3700.035.375918
TATCAAC526200.034.9591452
CCATAGG10350.034.605551
TAGGGTC10400.034.3662074
TCATAGG4250.033.6400532
GTACAGG8450.033.2028961
ATCAACG566050.032.4964683
AGTCGTG3150.032.108877
TCTACGG2056.91216E-1131.9648882
TCAACGC576650.031.9094474
AGTCGAC2255.456968E-1231.7311237
TCGACGG2500.030.9378439
CAACGCA595600.030.8511435
TGGATAT2700.030.84979
TGTACGG2351.0913936E-1130.4191972
ATGGATA2750.030.2887958
Produced by FastQC (version 0.11.5)