FastQCFastQC Report
Thu 26 May 2016
SRR935967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945142
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT76020.25811998199068165No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT64770.21992148426120034No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC59670.2026048319571688No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT58720.19937918103779037No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT53820.18274161313783852No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG42180.14321890082040187No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC34680.11775323566741433No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA31440.10675206832132372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88200.059.787081
GTATCAA149800.050.1177371
AGTAGTA15700.045.099822
GATAGCG18150.043.5897679
GAGTAGT23750.040.642841
GAGTAAG22050.039.7229421
TATCAAC189700.039.3330572
GATTGTG39350.039.0017859
AGGGTAA41500.038.987946
GAGTACG11600.037.4970861
GAGTTAG17550.037.346361
GGGGTAA68100.036.7743079
AGTAAGG16350.036.0283972
ATCAACG207350.035.7523543
TAAGGGG19700.035.6374324
GTAAGGG18400.035.5684933
TCAACGC208800.035.4185874
AGTAACG7100.035.1980362
GAGTCAG61850.034.4886361
GTATGGG14950.034.2759861