FastQCFastQC Report
Thu 26 May 2016
SRR935967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945142
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT76700.2604288689645525No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT71520.24284058289888907No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT61790.20980312664041326No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT57670.1958139879163721No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC50230.17055204808460847No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG46990.15955088073851786No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC37840.12848276925187307No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA31820.1080423286890751No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100400.063.8196951
GTATCAA170600.050.194811
GATAGCG15900.050.1401989
AGGGTAA43700.041.1153376
GAGTAAG22300.040.970881
GATTGTG40900.040.875349
AGTAGTA17200.039.8447342
TATCAAC220050.038.862622
GAGTTAG17950.037.9253041
GTAGTAG32950.036.971191
GAGTAGT25100.035.92461
GTGGTAT46100.035.6221771
ATCAACG239450.035.6144563
TCAACGC244500.034.878864
TGGTATC44600.034.7408182
GGGATAG24200.034.418437
GAGTACG12700.034.3247871
TAGTAGG35250.033.8121222
CAACGCA253600.033.6056445
GGATAGC24150.033.5042658