FastQCFastQC Report
Thu 26 May 2016
SRR935966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974980
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT130810.43970043496090727No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT121090.4070279464063624No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC120590.40534726283874173No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT115620.3886412681765928No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT101410.3408762411848147No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG83260.2798674276801861No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA61240.20585012336217387No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC51840.17425327229090615No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA50550.16991710868644494No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT43600.1465556070965183No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC38000.12773195113916733No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC33370.11216882130300036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT31950.062.0972441
GGTATCA83150.058.3975071
AGTAGTA27050.056.5225752
GAGTCAG77650.052.7125741
GAGTAAG24150.049.5881461
AGTCGTA7100.049.4367262
GTGGGGT89650.048.70087
AGGGTAA77800.048.6259236
TGGGGTA91800.048.4025548
GGGGTAA104900.048.3119359
GTATCAA138250.048.267171
GTTAACG2000.047.5933233
GATTGTG67900.047.4819
GATAGCG27800.045.3608749
GAGTACG13300.044.348871
AGTACGG7800.044.237382
GAGTAGG30950.043.8907661
GTAGTAG45700.043.8048321
GAGTTAG22750.041.9022671
GAGTCGT13200.041.5253031
Produced by FastQC (version 0.11.5)