FastQCFastQC Report
Thu 26 May 2016
SRR935966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974980
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT136830.4599358651150596No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT129160.4341541791877592No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT123290.4144229541038932No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT112720.37889330348439315No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC102740.3453468594746855No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG95450.3208424930587769No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC57560.19348029230448607No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA52020.1748583183752496No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA49770.16729524232095677No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT45780.15388338745134422No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC33710.11331168612898238No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG32350.10874022682505427No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA32320.10863938581099704No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA31540.10601751944550887No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT31480.1058158374173944No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG30080.10110992342805666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97700.067.421461
AGGGTAA74400.056.6979036
GATTGTG70450.053.625569
GAGTAGT32050.052.5458371
GTAGTAG46050.049.798791
GTATCAA165200.049.1638761
TAGTAGG46550.048.7753452
AGTAGTA25050.048.53082
GATAGCG27150.047.552039
GAGTCAG72300.046.751481
GAGTACG12100.046.3937761
TCGTAGG13700.046.108432
GAGTAAG22750.045.4131771
AGTACGG7800.044.3127252
TAGGGTA76400.043.312565
GAGTTAG20700.042.4096031
GGGATTG93600.041.3794067
GTAGGGT77600.041.3143544
TGGGGTA87700.041.246128
CGGGATT24850.040.9414676
Produced by FastQC (version 0.11.5)