FastQCFastQC Report
Thu 26 May 2016
SRR935965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892063
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT101560.5367685959716986No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC97320.5143591941705958No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT93370.49348251088890804No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT86270.4559573333446085No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG75850.40088517137114354No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT65320.3452316334075557No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA55830.2950747411687666No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC33010.17446564939962358No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC30150.1593498736564269No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC29240.15454030864722793No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA28030.14814517275587546No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG26340.1392131234530774No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG24370.12880120799360276No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG21500.11363257988766759No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT21200.11204700900551408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT24700.067.3027041
AGTAGTA21100.064.862882
GAGTCAG55450.064.687481
GTGGGGT64450.056.6691977
TGGGGTA67300.056.3906638
GGGGTAA78250.055.1891759
GAGTAAG17450.054.632341
GAGTCGT8000.054.3698461
GGTATCA54850.053.9889681
AGGGTAA64650.052.905516
GATTGTG62550.051.8287549
GAGTCCG7050.051.5544241
TAGTAGG38650.050.9599882
GAGTCGG10100.050.7343751
AGTCGTA5850.049.841492
TCAGTGG50300.049.3256724
GTAGTAG40500.049.137911
TACGGGA2800.048.8736464
GATAGCG18100.047.9817289
AGTCCGT3600.047.934292
Produced by FastQC (version 0.11.5)