FastQCFastQC Report
Thu 26 May 2016
SRR935965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892063
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT110390.58343723226975No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT97520.5154162414253648No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT90560.4786309969594036No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC84710.44771236475741033No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG84430.44623249860073366No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT74740.3950185591071756No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA45020.2379413370485021No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC33830.17879954314417648No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA29070.15364181848067426No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC28490.15057638144184415No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG28350.14983644836350588No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG27180.1436527219231072No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC25760.1361476864142473No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG24180.12779701310157218No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT23510.12425590479809605No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT20580.10877016251573017No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA19310.10205791244794703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT21200.061.4154971
AGTAGTA16050.061.2680472
GGTATCA58400.061.2591971
GATTGTG64750.059.6325579
GATAGCG19150.058.7180339
TCGTAGG9450.058.651112
AGGGTAA60350.058.461156
GAGTCGT6650.056.5817381
GTAGTAG36800.055.9923441
TAGTAGG36450.055.7547652
GAGTCAG47950.054.68061
CGGGATT21100.052.1650056
GAGTACG8500.051.996021
GAGTAAG14850.051.8824651
TACGCGG2200.051.4702222
GAGTTAG13750.051.2550471
GGGATTG77950.050.297667
CGGGATA6500.049.4276926
TACGGGA2100.048.243994
GGATAGC23700.047.947238
Produced by FastQC (version 0.11.5)