FastQCFastQC Report
Thu 26 May 2016
SRR935964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849197
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC196961.0651109643807555No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT180120.9740444095464139No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT166610.9009856710777706No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG160140.8659975113522247No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT148500.8030512703622168No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA112350.6075610116174751No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT95280.5152506736707879No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC64120.34674510071128173No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG54910.29693969869083714No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC54790.29629076837135254No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG52410.2834203170349076No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA47510.25692232898928563No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG47290.2557326234035638No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG45780.24756691688338234No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC39130.21160536167860966No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT37860.20473751579739746No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT37090.20057354624737114No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA36720.1985726777622936No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA35290.19083959145510188No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC34970.18910911060314287No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC34970.18910911060314287No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA33960.18364728041414732No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG29600.16006947880620614No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG29410.1590420058003555No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT29240.1581226878477523No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT27330.1477938802626221No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC27080.14644194209702915No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT25950.140331181588549No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG25910.1401148714820541No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT24840.13432857613331622No Hit
GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA22740.12297229554233541No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA22480.12156627985011872No Hit
GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA22130.1196735664182886No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA19690.1064786499221013No Hit
GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG19450.10518078928313208No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG19220.10393700617078655No Hit
GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA18850.10193613768570899No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA18790.10161167252596669No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC18660.10090866467985835No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA18540.10025973436037373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAG2600.086.9522252
GAGTAGT39000.086.604661
AGTAGTA32650.085.641672
GAGTAAG15500.081.091171
GAGTCAG84300.080.8390661
AGTCCGT4450.080.216132
GAGTTAG18400.077.37541
GAGTACG10350.074.245851
AGTCGTA7150.072.3908542
GAGTCCG9500.071.483211
GAGTCGT11600.069.839921
AGTTAGT13450.068.561432
AGTCGGT5600.067.992722
GAGTTCG6500.067.8174061
GAGTCGG13250.067.436991
GTGGGGT107800.067.3231667
GAGTAGG18600.066.935451
GAGTAAC17050.066.7316361
TGGGGTA113950.066.143298
GAGTTAA4800.065.774481
Produced by FastQC (version 0.11.5)